BupsA.17291.b

Luciferase-like monooxygenase

CENTER ID: BupsA.17291.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17291.b.A1.GE31045 Full length( BupsA.17291.b ) 1 424
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.2991
RefSeq: YP_334245.1
UniProt: Q3JQA8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKYSSRICAY FVGFLTVTVY YRTILRAARV TLTPLSPLPS SGISIRPPTR SGPLWHNRRR LLRTRIIDGP HRMRRPAFRP HANRSVVAMI PFSVLDLAPI PAGADAAQAL RHSVDLARHA ERLGYRRYWL AEHHNMPGIA SAATAVVIGH VAGATRTIRV GSGGVMLPNH APLVIAEQFG TLASLYPGRI DLGLGRAPGT DQTTARALRR DLIGSADSFP DDVVELQRYF AAPAAGQRVR AVPGAGLDVP IWLLGSSLFS AQLAAMLGLP FAFASHFAPD YLMRALDVYR AQFRPSAALD KPYAMVGVNV FAADTDDDAR RLFTSLQQQF LKLRRGTPGQ LPPPVESLDA LGATEQELAN VAHALSFAAV GSRDTVHERL RRLIAQTGAD ELIVAAQIFD HGARVRSYEI AAQVRDALRD EAGV
NT Sequence
ATGAAATATT CGAGTCGAAT CTGCGCCTAT TTCGTCGGAT TTCTTACGGT TACAGTCTAT TACAGAACAA TCCTTCGCGC CGCCCGCGTC ACGTTGACGC CCCTTTCGCC CCTGCCATCA TCAGGGATAT CCATCCGGCC GCCGACGCGA TCCGGACCGC TTTGGCACAA TCGACGCCGT CTGTTGCGTA CACGCATCAT CGATGGGCCG CACCGTATGC GCCGGCCCGC GTTCCGTCCC CATGCCAACC GTTCCGTCGT CGCCATGATC CCGTTTTCCG TTCTCGACCT CGCGCCGATT CCCGCCGGCG CCGACGCCGC CCAGGCGTTG CGCCATTCCG TCGACCTCGC GCGGCACGCC GAGCGCCTCG GCTATCGCCG CTACTGGCTC GCCGAGCACC ACAACATGCC CGGCATCGCG AGCGCGGCGA CCGCGGTCGT GATCGGCCAC GTCGCGGGCG CGACGCGGAC GATTCGCGTC GGCTCGGGCG GCGTGATGCT GCCGAACCAT GCGCCGCTCG TGATCGCCGA GCAGTTCGGC ACGCTCGCGT CGCTGTACCC GGGCCGCATC GATCTCGGTC TCGGGCGCGC GCCCGGCACC GATCAGACGA CGGCCCGCGC GCTGCGCCGC GACCTGATCG GCAGCGCCGA TTCGTTCCCC GACGACGTGG TGGAGTTGCA GCGCTACTTC GCCGCACCCG CCGCCGGCCA GCGCGTGCGC GCCGTGCCGG GCGCGGGGCT CGACGTGCCG ATCTGGCTGC TCGGCTCGAG CCTGTTCAGC GCGCAGCTCG CCGCGATGCT CGGGCTGCCG TTCGCGTTCG CTTCGCATTT CGCGCCGGAC TACCTGATGC GCGCGCTCGA CGTGTACCGC GCGCAGTTCC GGCCGTCCGC CGCGCTCGAC AAGCCGTATG CGATGGTCGG CGTGAACGTG TTCGCCGCCG ACACCGACGA CGACGCGCGA CGCCTGTTCA CGTCGCTGCA GCAGCAGTTC CTGAAGCTGC GGCGCGGCAC GCCCGGCCAA CTGCCGCCGC CCGTCGAATC GCTCGACGCG CTCGGCGCGA CCGAGCAGGA ACTCGCGAAC GTCGCGCATG CACTGTCGTT CGCCGCGGTC GGCTCGCGCG ACACCGTGCA CGAGCGGCTG CGGCGGTTGA TCGCGCAGAC GGGCGCGGAC GAGCTGATCG TCGCCGCGCA GATCTTCGAT CACGGCGCAC GGGTGCGCTC GTACGAGATC GCCGCGCAGG TGCGCGACGC GCTTCGCGAT GAAGCCGGGG TT
Details for BupsA.17291.b.A1.GE31045
HARVESTED ON: 12/14/2010
SEQUENCED ON: 1/13/2011
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 78
Validated AA Sequence
MKYSSRICAY FVGFLKVTVY YRTILRAARV TLTPLSPLPS SXISIRPPXX XGPLWHNRRR LLRTRIIDGP HRMRRPAFRP HANRSVVAMI PFSVLDLAPI PAGADAAQAL RHSVDLARHA ERLGYRRYWL AEHHNMPGIA SAATAVVIGH VAGATRTIRV GSGGVMLPNH APLVIAEQFG TLASLYPGRI DLGLGRAPGT DQTTARALRR DLIGSADSFP DDVVELQRYF AAPAAGQRVR AVPGAGXDVP IWLLGSSLFS AQLXXMLGLP FAXASHFAXX YLMXALXXYX XXXXXPXXSX SRXRXXXXTX SXXXRXXXXX XXXXXXAVXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
cnnnnnnnng nnnnnnnnnn angcggnnna gccatgtnnn nnnnnctcct nngnnnnnnn nnnnnnnnna tcanntgggt accctggaag ctcagaccca gggtcctggt tcgatgaaat attcgagtcg aatctgcgcc tatttcgtcg gatttcttaa ggttacagtc tattacagaa caatccttcg cgccgcccgc gtcacgttga cgcccctttc gcccctgcca tcntcnngga tatccatccg gccgccgann nnnnccggac cgctttggca caatcgacgc cgtctgttgc gtacacgcat catcgatggg ccgcaccgta tgcgccggcc cgcgttccgt ccccatgcca accgttccgt cgtcgccatg atcccgtttt ccgttctcga cctcgcgccg attcccgccg gcgccgacgc cgcccaggcg ttgcgccatt ccgtcgacct cgcgcggcac gccgagcgcc tcggctatcg ccgctactgg ctcgccgagc accacaacat gcccggcatc gcgagcgcgg cgaccgcggt cgtgatcggc cacgtcgcgg gcgcgacgcg gacgattcgc gtcggctcgg gcggcgtgat gctgccgaac catgcgccgc tcgtgatcgc cgagcagttc ggcacgctcg cgtcgctgta cccgggccgc atcgatctcg gtctcgggcg cgcgcccggc accgatcaga cgacggcccg cgcgctgcgc cgcgacctga tcggcagcgc cgattcgttc cccgacgacg tggtggagtt gcagcgctac ttcgccgcac ccgccgccgg ncagcgcgtg cgcgccgtgc cgggcgcggg gntcgacgtg ccgatctggc tgctcggctc gagcctgttc agcgcgcagc tcgncncgat gctcgggctg ccnttcgcgn tcgcttcgca tttcgcnncg gantacctga tgcnngcgct cganntgtac cnnnnnnnnt ncnngcngcc nncnnnctcg annagccgnn tgcganggnn gnngngnacn nnntcnncna nnnnncgacn nnncnngann nnngntnnnn nnnnnncann ngcagtncnn nnnnnnnnnn gnnnnnnnnn nnnnnnnnnn nnnnnnncnn nnnnnnnnnn nnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKYSSRICA YFVGFLTVTV YYRTILRAAR VTLTPLSPLP SSGISIRPPT RSGPLWHNRR RLLRTRIIDG PHRMRRPAFR PHANRSVVAM IPFSVLDLAP IPAGADAAQA LRHSVDLARH AERLGYRRYW LAEHHNMPGI ASAATAVVIG HVAGATRTIR VGSGGVMLPN HAPLVIAEQF GTLASLYPGR IDLGLGRAPG TDQTTARALR RDLIGSADSF PDDVVELQRY FAAPAAGQRV RAVPGAGLDV PIWLLGSSLF SAQLAAMLGL PFAFASHFAP DYLMRALDVY RAQFRPSAAL DKPYAMVGVN VFAADTDDDA RRLFTSLQQQ FLKLRRGTPG QLPPPVESLD ALGATEQELA NVAHALSFAA VGSRDTVHER LRRLIAQTGA DELIVAAQIF DHGARVRSYE IAAQVRDALR DEAGV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaatattcg agtcgaatct gcgcctattt cgtcggattt cttacggtta cagtctatta cagaacaatc cttcgcgccg cccgcgtcac gttgacgccc ctttcgcccc tgccatcatc agggatatcc atccggccgc cgacgcgatc cggaccgctt tggcacaatc gacgccgtct gttgcgtaca cgcatcatcg atgggccgca ccgtatgcgc cggcccgcgt tccgtcccca tgccaaccgt tccgtcgtcg ccatgatccc gttttccgtt ctcgacctcg cgccgattcc cgccggcgcc gacgccgccc aggcgttgcg ccattccgtc gacctcgcgc ggcacgccga gcgcctcggc tatcgccgct actggctcgc cgagcaccac aacatgcccg gcatcgcgag cgcggcgacc gcggtcgtga tcggccacgt cgcgggcgcg acgcggacga ttcgcgtcgg ctcgggcggc gtgatgctgc cgaaccatgc gccgctcgtg atcgccgagc agttcggcac gctcgcgtcg ctgtacccgg gccgcatcga tctcggtctc gggcgcgcgc ccggcaccga tcagacgacg gcccgcgcgc tgcgccgcga cctgatcggc agcgccgatt cgttccccga cgacgtggtg gagttgcagc gctacttcgc cgcacccgcc gccggccagc gcgtgcgcgc cgtgccgggc gcggggctcg acgtgccgat ctggctgctc ggctcgagcc tgttcagcgc gcagctcgcc gcgatgctcg ggctgccgtt cgcgttcgct tcgcatttcg cgccggacta cctgatgcgc gcgctcgacg tgtaccgcgc gcagttccgg ccgtccgccg cgctcgacaa gccgtatgcg atggtcggcg tgaacgtgtt cgccgccgac accgacgacg acgcgcgacg cctgttcacg tcgctgcagc agcagttcct gaagctgcgg cgcggcacgc ccggccaact gccgccgccc gtcgaatcgc tcgacgcgct cggcgcgacc gagcaggaac tcgcgaacgt cgcgcatgca ctgtcgttcg ccgcggtcgg ctcgcgcgac accgtgcacg agcggctgcg gcggttgatc gcgcagacgg gcgcggacga gctgatcgtc gccgcgcaga tcttcgatca cggcgcacgg gtgcgctcgt acgagatcgc cgcgcaggtg cgcgacgcgc ttcgcgatga agccggggtt taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa