SSGCID: Target Page

BupsA.17288.d

Type-1 fimbrial protein, A subunit

CENTER ID:	BupsA.17288.d
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17288.d.A2.GE31032	Mature protein( BupsA.17288.d )		34	323

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.2329
RefSeq:	YP_333625.1
UniProt:	Q3JS28

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRLKRERRAR RSRLPAAAGG LWLVVAALPP GARAATCEGD KTLVTLPAIA VAADAPVGTV LWRQKGIAFS TYCTLGWFDT SNIYVWRADL RSALQPYGLT FWLTYGGQGG NTALQIKEPM VVDLGGKAGY ASGSVDLELR KTGVTPAQGV VGAADIPAFY LDSNTNYNKG SHYIRGLTNI SFVSYTCDID TGSRSMNVPL GDVRVDRFSG IGSTFADRNF SIGMTCTQPA GTYDIALTFS ATADSSGAPG VLAITQGASS ASGVGIQLLM NGSPVTFGAV LDAGSATAGA TLTIPMTARY YQTGSVVTPG AANGIATFAV SYK

NT Sequence

ATGCGATTGA AACGCGAACG GCGTGCGCGG CGCTCCCGCC TGCCCGCGGC GGCAGGCGGC CTGTGGCTTG TCGTTGCCGC GCTGCCGCCC GGCGCACGGG CAGCGACTTG CGAAGGCGAC AAGACACTCG TCACGCTTCC GGCCATCGCG GTCGCGGCCG ACGCGCCGGT GGGAACGGTG CTGTGGCGTC AGAAGGGGAT CGCTTTCAGC ACCTATTGCA CGTTGGGATG GTTCGACACC AGCAACATTT ATGTCTGGCG CGCCGACCTG CGCTCGGCGC TGCAGCCATA TGGGCTGACG TTCTGGCTGA CTTACGGAGG GCAGGGCGGC AACACCGCGC TGCAAATCAA GGAGCCGATG GTCGTCGATC TCGGCGGAAA GGCCGGTTAT GCGAGCGGCT CCGTCGACCT GGAACTGAGG AAGACGGGCG TGACGCCGGC GCAAGGCGTC GTCGGCGCGG CGGACATCCC CGCGTTCTAT CTCGATAGCA ATACGAACTA CAACAAAGGC TCGCACTACA TCCGCGGGCT GACCAACATT TCGTTCGTCT CCTATACCTG CGACATCGAT ACGGGGTCGC GCAGCATGAA CGTGCCGCTC GGCGACGTGC GCGTCGATCG CTTCAGCGGC ATCGGCTCCA CCTTCGCGGA TCGGAATTTC AGCATCGGCA TGACGTGCAC GCAGCCGGCC GGCACGTACG ATATCGCGCT GACGTTTTCC GCGACGGCGG ACAGCTCCGG CGCACCGGGC GTGCTCGCGA TCACGCAGGG GGCGTCTTCC GCGTCCGGAG TCGGCATTCA GTTGCTGATG AACGGCTCGC CGGTGACTTT CGGCGCCGTC CTCGACGCGG GCAGCGCGAC CGCGGGCGCG ACGCTGACGA TCCCGATGAC GGCACGCTAT TATCAGACCG GCAGTGTCGT GACGCCGGGC GCGGCGAACG GGATCGCAAC ATTCGCCGTC AGCTACAAA

Details for BupsA.17288.d.A2.GE31032

HARVESTED ON:	12/14/2010
SEQUENCED ON:	12/20/2010
EXPECTED MW:	32kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	47
PERCENT COVERAGE:	21

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAARVTFAM KPGFNKQHEQ VLQPSFSRIR LLTKPERKLS WLLPPXXX

Validated NT Sequence

attttgttta actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggcc gcccgcgtca cgttcgcgat gaagccgggg tttaataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgcnna nnnnctagca annttatgtc tggcgcgccg acctgcgctc ggcgctgcag ccatatgggc tgacgttctg gctgacttac ggagggcagg gcggcaacac cgcgctgcaa atcaaggagc cgatggtcgt cgatctcggc ggaaaggccg gttatgcgag cggctccgtc gacctggaac tgaggaagac gggcgtgacg ccggcgcaag gcgtcgtcgg cgcggcggac atccccgcgt tctatctcga tagcaatacg aactacaaca aaggctcgca ctacatccgc gggctgacca acatttcgtt cgtctcctat acctgcgaca tcgatacggg gtcgcgcagc atgaacgtgc cgctcggcga cgtgcgcgtc gatcgcttca gcggcatcgg ctccaccttc gcggatcgga atttcagcat cggcatgacg tgcacgcagc cggccggcac gtacgatatc gcgctgacgt tttccgcgac ggcggacagc tccggcgcac cgggcgtgct cgcgatcacg cagggggcgt cttccgcgtc cggagtcggc attcagttgc tgatgaacgg ctcgccggtg actttcggcg ccgtcctcga cgcgggcagc gcgacngcgg gngcgacgct gacgatcccg atgacggnnc gctattatca gaccggcagt gtcgtgacgc cgggcgcgga acgggatcgc aacattcgcc gtcagctaca aanaataaac agnncgaacn agttctgcag nnnagctnnt cgnngatcnn ntgctannaa agnccnaaan nnagctgann nnnctgctgc cncnnntnnn nnnnnnnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAATCEGDK TLVTLPAIAV AADAPVGTVL WRQKGIAFST YCTLGWFDTS NIYVWRADLR SALQPYGLTF WLTYGGQGGN TALQIKEPMV VDLGGKAGYA SGSVDLELRK TGVTPAQGVV GAADIPAFYL DSNTNYNKGS HYIRGLTNIS FVSYTCDIDT GSRSMNVPLG DVRVDRFSGI GSTFADRNFS IGMTCTQPAG TYDIALTFSA TADSSGAPGV LAITQGASSA SGVGIQLLMN GSPVTFGAVL DAGSATAGAT LTIPMTARYY QTGSVVTPGA ANGIATFAVS YK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagcgact tgcgaaggcg acaagacact cgtcacgctt ccggccatcg cggtcgcggc cgacgcgccg gtgggaacgg tgctgtggcg tcagaagggg atcgctttca gcacctattg cacgttggga tggttcgaca ccagcaacat ttatgtctgg cgcgccgacc tgcgctcggc gctgcagcca tatgggctga cgttctggct gacttacgga gggcagggcg gcaacaccgc gctgcaaatc aaggagccga tggtcgtcga tctcggcgga aaggccggtt atgcgagcgg ctccgtcgac ctggaactga ggaagacggg cgtgacgccg gcgcaaggcg tcgtcggcgc ggcggacatc cccgcgttct atctcgatag caatacgaac tacaacaaag gctcgcacta catccgcggg ctgaccaaca tttcgttcgt ctcctatacc tgcgacatcg atacggggtc gcgcagcatg aacgtgccgc tcggcgacgt gcgcgtcgat cgcttcagcg gcatcggctc caccttcgcg gatcggaatt tcagcatcgg catgacgtgc acgcagccgg ccggcacgta cgatatcgcg ctgacgtttt ccgcgacggc ggacagctcc ggcgcaccgg gcgtgctcgc gatcacgcag ggggcgtctt ccgcgtccgg agtcggcatt cagttgctga tgaacggctc gccggtgact ttcggcgccg tcctcgacgc gggcagcgcg accgcgggcg cgacgctgac gatcccgatg acggcacgct attatcagac cggcagtgtc gtgacgccgg gcgcggcgaa cgggatcgca acattcgccg tcagctacaa ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa