SSGCID: Target Page

BupsA.17287.e

Thioesterase domain protein

CENTER ID:	BupsA.17287.e
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17287.e.A1.GE30976	Full length( BupsA.17287.e )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.660
RefSeq:	YP_332059.1
UniProt:	Q3JWJ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLPGTLTIPD RREHARHRLL MFHHACGSAT NYFRWAAAFA PHIEVWLVEL PGRGRSLRHG AFDALPPLRD YLRELAPELP ADYSIFGHSM GALVAYCFAQ DMMQLGRAPR WLGVSGADSP FAASRRRAFP VHQRPDAAIV EHLATLGGTP PEVFAHDELR AMLLQLAKAD FRIVEAFFPL ASATPLPVPV TVFAGAQDTV LSEGGLQDWQ RASTLPIERE TFDGGHFYLL DTPCAIHDAI GAALARHERQ ASVPAVL

NT Sequence

TTGCTGCCCG GCACCCTCAC CATTCCCGAC AGGCGCGAAC ACGCGCGGCA CCGGCTGCTG ATGTTCCATC ACGCATGCGG CTCCGCGACG AACTATTTTC GATGGGCCGC CGCGTTTGCG CCGCATATCG AAGTCTGGCT CGTCGAGCTG CCGGGGCGGG GCCGCAGCCT CAGGCACGGT GCGTTCGACG CACTGCCGCC GCTGCGCGAC TACCTGCGCG AGCTCGCCCC CGAACTCCCC GCCGATTATT CGATCTTCGG CCATAGCATG GGCGCGCTCG TCGCGTACTG CTTCGCGCAA GACATGATGC AGCTCGGGCG CGCGCCGCGC TGGCTCGGCG TATCGGGCGC CGATTCACCG TTCGCCGCGT CGCGGCGCCG CGCGTTCCCG GTCCATCAGC GCCCCGATGC GGCGATCGTC GAGCACCTCG CGACGCTCGG CGGCACGCCG CCTGAAGTTT TCGCGCACGA CGAGTTGCGC GCGATGCTGC TGCAACTCGC GAAGGCCGAC TTCCGGATTG TCGAGGCGTT CTTTCCGCTC GCGTCGGCCA CGCCGCTGCC CGTGCCCGTC ACGGTCTTCG CCGGCGCGCA AGACACGGTG CTGTCCGAGG GCGGGCTGCA GGACTGGCAG CGCGCGTCGA CGCTGCCGAT CGAGCGCGAA ACGTTCGACG GCGGGCATTT CTACCTGCTC GATACGCCGT GCGCCATCCA TGACGCGATT GGTGCGGCGC TGGCGAGACA CGAACGGCAG GCGAGCGTCC CCGCCGTTCT A

Details for BupsA.17287.e.A1.GE30976

HARVESTED ON:	11/23/2010
SEQUENCED ON:	12/6/2010
EXPECTED MW:	30kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLLPGTLTI PDRREHARHR LLMFHHACGS ATNYFRWAAA FAPHIEVWLV ELPGRGRSLR HGAFDALPPL RDYLRELAPE LPADYSIFGH SMGALVAYCF AQDMMQLGRA PRWLGVSGAD SPFAASRRRA FPVHQRPDAA IVEHLATLGG TPPEVFAHDE LRAMLLQLAK ADFRIVEAFF PLASATPLPV PVTVFAGAQD TVLSEGGLQD WQRASTLPIE RETFDGGHFY LLDTPCAIHD AIGAALARHE RQASVPAVL

Validated NT Sequence

ttattataga acggcgggga cgctcgcctg ccgttcgtgt ctcgccagcg ccgcaccaat cgcgtcatgg atggcgcacg gcgtatcgag caggtagaaa tgcccgccgt cgaacgtttc gcgctcgatc ggcagcgtag acgcgcgctg ccagtcctgc agcccgccct cggacagcac cgtgtcttgc gcgccggcga agaccgtgac gggcacggga agcggcgtgg ccgacgcgag cggaaagaac gcctcgacaa tccggaagtc ggccttcgcg agttgcagca gcatcgcgcg caactcgtcg tgcgcgaaaa cttcaggcgg cgtgccgccg agcgtcgcga ggtgctcgac gatcgccgca tcggggcgct gatggaccgg gaacgcgcgg cgccgcgacg cggcgaacgg tgaatcggcg cccgatacgc cgagccagcg cggcgcgcgc ccgagctgca tcatgtcttg cgcgaagcag tacgcgacga gcgcgcccat gctatggccg aagatcgaat aatcggcggg gagttcgggg gcgagctcgc gcaggtagtc gcgcagcggc ggcagtgcgt cgaacgcacc gtgcctgagg ctgcggcccc gccccggcag ctcgacgagc cagacttcga tatgcggcgc aaacgcggcg gcccatcgaa aatagttcgt cgcggagccg catgcgtgat ggaacatcag cagccggtgc cgcgcgtgtt cgcgcctgtc gggaatggtg agggtgccgg gcagcaacat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgctgccc ggcaccctca ccattcccga caggcgcgaa cacgcgcggc accggctgct gatgttccat cacgcatgcg gctccgcgac gaactatttt cgatgggccg ccgcgtttgc gccgcatatc gaagtctggc tcgtcgagct gccggggcgg ggccgcagcc tcaggcacgg tgcgttcgac gcactgccgc cgctgcgcga ctacctgcgc gagctcgccc ccgaactccc cgccgattat tcgatcttcg gccatagcat gggcgcgctc gtcgcgtact gcttcgcgca agacatgatg cagctcgggc gcgcgccgcg ctggctcggc gtatcgggcg ccgattcacc gttcgccgcg tcgcggcgcc gcgcgttccc ggtccatcag cgccccgatg cggcgatcgt cgagcacctc gcgacgctcg gcggcacgcc gcctgaagtt ttcgcgcacg acgagttgcg cgcgatgctg ctgcaactcg cgaaggccga cttccggatt gtcgaggcgt tctttccgct cgcgtcggcc acgccgctgc ccgtgcccgt cacggtcttc gccggcgcgc aagacacggt gctgtccgag ggcgggctgc aggactggca gcgcgcgtcg acgctgccga tcgagcgcga aacgttcgac ggcgggcatt tctacctgct cgatacgccg tgcgccatcc atgacgcgat tggtgcggcg ctggcgagac acgaacggca ggcgagcgtc cccgccgttc tataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa