SSGCID: Target Page

BupsA.17287.d

Putative thioesterase

CENTER ID:	BupsA.17287.d
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17287.d.A1.GE30975	Full length( BupsA.17287.d )		1	291

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.4091
RefSeq:	YP_335292.1
UniProt:	Q3JMB1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSIPVRLDR FAPAADPAAP ADSAAATDGA PAAAFPATAA KAAIRTLVAP SPGGAHVLCI PWAGASIERF FAWKRHLPAG VGLSGVQLPG RGARAHEPSP TDLVALLDEI AAEYLALRDP PRILFGHSFG ALIAFEIATR VSRAAGQAGG DPFVRLVVSG LSAPSVIAEE ERIAHLDDDA FSAQVHALNG MPPEIARSPE SLRYFMTVLR CDFRLYESYA FDANRPPLRC PIAVCSGRDD PSVSEHGLNA WAALTTGECR RHDFDGDHFF IADHAAAMLG LALDAESTLA G

NT Sequence

ATGACCAGCA TCCCCGTTAG ACTCGATCGG TTCGCGCCGG CGGCCGACCC GGCCGCCCCG GCCGATTCGG CCGCCGCCAC GGACGGCGCC CCGGCGGCGG CGTTTCCCGC CACGGCCGCG AAGGCCGCGA TCCGCACGCT GGTTGCGCCG TCGCCCGGCG GCGCGCACGT GCTGTGCATC CCATGGGCGG GCGCGAGCAT CGAGCGCTTC TTCGCGTGGA AGCGGCACCT GCCGGCGGGC GTCGGCTTGT CGGGCGTCCA GTTGCCGGGG CGCGGCGCGC GTGCGCACGA GCCGTCGCCG ACGGATCTCG TCGCGCTGCT CGACGAGATC GCGGCCGAGT ACCTGGCGCT GCGCGACCCG CCGCGCATCC TGTTCGGGCA CAGCTTCGGC GCGCTGATCG CGTTCGAGAT CGCGACGCGC GTGAGCCGGG CGGCCGGCCA GGCGGGCGGC GATCCGTTCG TGCGGCTCGT CGTGTCGGGG CTGTCCGCGC CGAGCGTGAT CGCCGAGGAA GAGCGCATCG CCCATCTCGA CGACGACGCG TTCAGCGCGC AAGTGCACGC GCTCAACGGC ATGCCGCCCG AAATCGCGCG CAGCCCCGAA TCGCTGCGCT ATTTCATGAC CGTCCTGCGC TGCGATTTCC GGCTGTACGA ATCCTATGCG TTCGACGCGA ATCGCCCGCC GCTGCGCTGC CCGATCGCCG TGTGCTCCGG CCGCGACGAT CCGAGCGTGT CGGAGCACGG GCTGAACGCG TGGGCGGCGC TCACGACGGG CGAGTGCCGG CGTCACGATT TCGACGGCGA TCATTTCTTC ATCGCGGACC ACGCGGCCGC GATGCTGGGC CTCGCGCTGG ACGCCGAAAG CACGCTCGCC GGC

Details for BupsA.17287.d.A1.GE30975

HARVESTED ON:	11/23/2010
SEQUENCED ON:	12/6/2010
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	24
PERCENT COVERAGE:	51

Validated AA Sequence

MAHHHHHHMG TXXAQTQGPG SMTSIXVRLD RFAPXXXXXX XXXXSAXXXX XXXXXXXXXX XXXPXXXXXX XXXXXXXXXX XCXXXXXGSG XXXSXXGXEL XXXXXXXXXX XXXAXXXXXX XXHAXXXAXX XXSXXAXXXX XXXLSXXLXS XXRXXXXMXX LX

Validated NT Sequence

attttgnnnn actttaagaa ggagatatac catggctcat caccatcacc atcatatggg tacccngnnn gctcagaccc agggtcctgg ttcgatgacc agcatcnccg ttagactcga tcggttcgcg ccggnggnng nngnngnngn ntgnnnnnnn nnntcagcna nnnctannnn nnnnnnnnnn tnannnnnnn nagnnnnnnn nnnnannnng ccannnnnan ctagnaannn cnnnnnnnnn ngcannnncn gngngnnnnn nntctgcngc cnngnnncan naggatccgg nagnnaanna agccnganng gancngagtt ggntgnngnc ncannnnann nnnnnnnann annnnnnngn gcgnnnnnnn tgnnnnnnnt ngnanancat gcnntancan nngcnnnnan nnnnnnaagt gntnctgcnn ntntgcnnnn nnnanttnan ctatcncnnt tnctgngctc nnnntnncgg tnnnnnnnnn tgatgncnnn nctgnnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTSIPVRLD RFAPAADPAA PADSAAATDG APAAAFPATA AKAAIRTLVA PSPGGAHVLC IPWAGASIER FFAWKRHLPA GVGLSGVQLP GRGARAHEPS PTDLVALLDE IAAEYLALRD PPRILFGHSF GALIAFEIAT RVSRAAGQAG GDPFVRLVVS GLSAPSVIAE EERIAHLDDD AFSAQVHALN GMPPEIARSP ESLRYFMTVL RCDFRLYESY AFDANRPPLR CPIAVCSGRD DPSVSEHGLN AWAALTTGEC RRHDFDGDHF FIADHAAAML GLALDAESTL AG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccagcatc cccgttagac tcgatcggtt cgcgccggcg gccgacccgg ccgccccggc cgattcggcc gccgccacgg acggcgcccc ggcggcggcg tttcccgcca cggccgcgaa ggccgcgatc cgcacgctgg ttgcgccgtc gcccggcggc gcgcacgtgc tgtgcatccc atgggcgggc gcgagcatcg agcgcttctt cgcgtggaag cggcacctgc cggcgggcgt cggcttgtcg ggcgtccagt tgccggggcg cggcgcgcgt gcgcacgagc cgtcgccgac ggatctcgtc gcgctgctcg acgagatcgc ggccgagtac ctggcgctgc gcgacccgcc gcgcatcctg ttcgggcaca gcttcggcgc gctgatcgcg ttcgagatcg cgacgcgcgt gagccgggcg gccggccagg cgggcggcga tccgttcgtg cggctcgtcg tgtcggggct gtccgcgccg agcgtgatcg ccgaggaaga gcgcatcgcc catctcgacg acgacgcgtt cagcgcgcaa gtgcacgcgc tcaacggcat gccgcccgaa atcgcgcgca gccccgaatc gctgcgctat ttcatgaccg tcctgcgctg cgatttccgg ctgtacgaat cctatgcgtt cgacgcgaat cgcccgccgc tgcgctgccc gatcgccgtg tgctccggcc gcgacgatcc gagcgtgtcg gagcacgggc tgaacgcgtg ggcggcgctc acgacgggcg agtgccggcg tcacgatttc gacggcgatc atttcttcat cgcggaccac gcggccgcga tgctgggcct cgcgctggac gccgaaagca cgctcgccgg ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa