SSGCID: Target Page

BupsA.17287.a

Pyochelin biosynthetic protein

CENTER ID:	BupsA.17287.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17287.a.A1.GE32563	Full length( BupsA.17287.a )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.6311
RefSeq:	YP_337298.1
UniProt:	Q3JGK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKPPPPHAGW VRELRLSPCP RAQLVCMPHA GGAASFFRGW AGALPWDLDL LALQYPGRED RFGEPCARSI DALAGPAAEA LVEYARQPLV LFGHSLGAAL AYEVALRLEQ RGAPPLYVAV SSHPPPHRQR ASSLHLQSDA ALLNDVARLS GEHAALLDDP ALRAIYLPMI RDDYRAIETY RRERPPMLDA PLGVMLPLAD PELDRDEAQA WQDVASRPIR VTTFDGDHFY LRHAYPALIA HIAGQIDRSL RPLKEHS

NT Sequence

ATGAAGCCGC CGCCTCCGCA CGCCGGCTGG GTTCGCGAAC TCAGGCTGTC GCCCTGCCCG CGCGCGCAGC TCGTCTGCAT GCCGCACGCG GGCGGCGCCG CAAGTTTCTT TCGCGGCTGG GCAGGCGCGC TGCCGTGGGA TCTCGATCTG CTCGCGCTTC AATATCCGGG CCGCGAGGAC CGCTTCGGCG AACCGTGCGC GCGCTCGATC GACGCGCTCG CCGGCCCCGC CGCCGAAGCG CTCGTCGAGT ACGCGCGCCA GCCGCTCGTG CTGTTCGGCC ACAGCCTGGG CGCCGCGCTC GCCTACGAAG TCGCGTTGCG CCTCGAGCAG CGCGGCGCGC CGCCGCTGTA TGTCGCGGTC TCGTCGCATC CGCCGCCGCA CCGGCAGCGC GCGTCGAGCC TGCACCTGCA ATCCGACGCC GCGCTGCTGA ACGACGTCGC GCGGCTGTCC GGCGAACACG CGGCGCTGCT CGACGATCCG GCGCTGCGCG CGATCTATCT GCCGATGATC CGCGACGACT ATCGCGCGAT CGAGACCTAC CGGCGCGAGC GGCCGCCGAT GCTCGACGCG CCGCTCGGCG TGATGCTGCC GCTCGCCGAT CCCGAGCTCG ACCGCGACGA AGCGCAGGCC TGGCAGGACG TCGCGTCCCG GCCGATCCGC GTGACGACGT TCGACGGCGA CCATTTCTAT CTGCGCCACG CGTACCCGGC GCTCATCGCG CACATCGCCG GGCAGATCGA CCGCTCGCTG CGCCCACTCA AGGAACACTC A

Details for BupsA.17287.a.A1.GE32563

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMKPPPPH AGWVRELRLS PCPRAQLVCM PHAGGAASFF RGWAGALPWD LDLLALQYPG REDRFGEPCA RSIDALAGPA AEALVEYARQ PLVLFGHSLG AALAYEVALR LEQRGAPPLY VAVSSHPPPH RQRASSLHLQ SDAALLNDVA RLSGEHAALL DDPALRAIYL PMIRDDYRAI ETYRRERPPM LDAPLGVMLP LADPELDRDE AQAWQDVASR PIRVTTFDGD HFYLRHAYPA LIAHIAGQID RSLRPLK

Validated NT Sequence

ttccttgagt gggcgcagcg agcggtcgat ctgcccggcg atgtgcgcga tgagcgccgg gtacgcgtgg cgcagataga aatggtcgcc gtcgaacgtc gtcacgcgga tcggccggga cgcgacgtcc tgccaggcct gcgcttcgtc gcggtcgagc tcgggatcgg cgagcggcag catcacgccg agcggcgcgt cgagcatcgg cggccgctcg cgccggtagg tctcgatcgc gcgatagtcg tcgcggatca tcggcagata gatcgcgcgc agcgccggat cgtcgagcag cgccgcgtgt tcgccggaca gccgcgcgac gtcgttcagc agcgcggcgt cggattgcag gtgcaggctc gacgcgcgct gccggtgcgg cggcggatgc gacgagaccg cgacatacag cggcggcgcg ccgcgctgct cgaggcgcaa cgcgacttcg taggcgagcg cggcgcccag gctgtggccg aacagcacga gcggctggcg cgcgtactcg acgagcgctt cggcggcggg gccggcgagc gcgtcgatcg agcgcgcgca cggttcgccg aagcggtcct cgcggcccgg atattgaagc gcgagcagat cgagatccca cggcagcgcg cctgcccagc cgcgaaagaa acttgcggcg ccgcccgcgt gcggcatgca gacgagctgc gcgcgcgggc agggcgacag cctgagttcg cgaacccagc cggcgtgcgg aggcggcggc ttcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagcca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKPPPPHAG WVRELRLSPC PRAQLVCMPH AGGAASFFRG WAGALPWDLD LLALQYPGRE DRFGEPCARS IDALAGPAAE ALVEYARQPL VLFGHSLGAA LAYEVALRLE QRGAPPLYVA VSSHPPPHRQ RASSLHLQSD AALLNDVARL SGEHAALLDD PALRAIYLPM IRDDYRAIET YRRERPPMLD APLGVMLPLA DPELDRDEAQ AWQDVASRPI RVTTFDGDHF YLRHAYPALI AHIAGQIDRS LRPLKEHS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagccgccg cctccgcacg ccggctgggt tcgcgaactc aggctgtcgc cctgcccgcg cgcgcagctc gtctgcatgc cgcacgcggg cggcgccgca agtttctttc gcggctgggc aggcgcgctg ccgtgggatc tcgatctgct cgcgcttcaa tatccgggcc gcgaggaccg cttcggcgaa ccgtgcgcgc gctcgatcga cgcgctcgcc ggccccgccg ccgaagcgct cgtcgagtac gcgcgccagc cgctcgtgct gttcggccac agcctgggcg ccgcgctcgc ctacgaagtc gcgttgcgcc tcgagcagcg cggcgcgccg ccgctgtatg tcgcggtctc gtcgcatccg ccgccgcacc ggcagcgcgc gtcgagcctg cacctgcaat ccgacgccgc gctgctgaac gacgtcgcgc ggctgtccgg cgaacacgcg gcgctgctcg acgatccggc gctgcgcgcg atctatctgc cgatgatccg cgacgactat cgcgcgatcg agacctaccg gcgcgagcgg ccgccgatgc tcgacgcgcc gctcggcgtg atgctgccgc tcgccgatcc cgagctcgac cgcgacgaag cgcaggcctg gcaggacgtc gcgtcccggc cgatccgcgt gacgacgttc gacggcgacc atttctatct gcgccacgcg tacccggcgc tcatcgcgca catcgccggg cagatcgacc gctcgctgcg cccactcaag gaacactcat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa