BupsA.17285.e

Extracellular solute-binding protein

CENTER ID: BupsA.17285.e
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystal structure
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17285.e.A2.GE32559 Mature protein( BupsA.17285.e ) 24 271
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17285.e.A2.PS01145 Mature protein( BupsA.17285.e ) 24 271
BupsA.17285.e.A2.PS01146 Mature protein( BupsA.17285.e ) 24 271
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127777
PATRIC ID: fig|320372.6.peg.2126
RefSeq: YP_333446.1
UniProt: Q3JSK7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFKPFATWL LGLSLVAAHA AAHADRLDDI RKAGVLRVAT FDSNPPFGFV DPRTNRIVGL DVDYAQALAD RLGVKLKLQP TNPANRIPFL TSGKVDLVLA NFTITDERAK QVDFSVPYFA SGQQFLAKKG VLKSADQLNR LRIGADKGTT NEVTLREKYP QATIVAYDDT PFAFAALRAG NVQAITQDGP KLIGLLANVP DKQNYEVPAF SISNDYMGVG APKGEPRLVA FVNETLTDLE ASGRAGRIYD AWFGPNTKTP LARIFKIGDK R
NT Sequence
ATGAAATTCA AGCCATTCGC TACCTGGCTC CTCGGTTTGT CGCTCGTCGC CGCGCATGCG GCCGCGCACG CGGATCGTCT CGACGATATC CGGAAGGCCG GCGTGCTGCG CGTCGCGACG TTCGACAGCA ACCCGCCGTT CGGCTTCGTC GATCCGAGGA CGAACCGGAT CGTCGGCCTC GACGTCGACT ATGCGCAGGC GCTCGCCGAC CGGCTCGGCG TCAAGCTGAA GCTGCAGCCG ACCAATCCCG CGAACCGCAT TCCGTTCCTG ACCTCGGGCA AGGTCGATCT CGTGCTCGCG AACTTCACGA TCACCGACGA GCGTGCGAAG CAGGTCGACT TCAGCGTGCC GTATTTCGCG TCGGGGCAGC AGTTTCTCGC GAAGAAGGGC GTGCTGAAAT CGGCCGATCA GTTGAACCGC CTGCGCATCG GCGCCGACAA GGGTACGACG AACGAGGTCA CGCTGCGCGA GAAATATCCG CAGGCGACGA TCGTCGCCTA CGACGACACG CCGTTCGCAT TCGCCGCGCT GCGCGCGGGC AACGTGCAGG CGATCACGCA GGACGGCCCG AAGCTGATCG GCCTGCTCGC GAACGTGCCG GACAAGCAGA ACTACGAAGT GCCGGCGTTC TCGATCTCGA ACGATTACAT GGGCGTCGGC GCGCCGAAGG GCGAGCCGCG GCTCGTCGCG TTCGTCAACG AAACGCTGAC GGATCTCGAG GCGAGCGGCC GCGCGGGCCG GATCTACGAC GCATGGTTCG GGCCGAATAC GAAGACGCCG CTCGCGCGGA TCTTCAAGAT CGGCGACAAG CGC
Details for BupsA.17285.e.A2.GE32559
HARVESTED ON: 5/17/2011
SEQUENCED ON: 5/23/2011
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADRLDDIR KAGVLRVATF DSNPPFGFVD PRTNRIVGLD VDYAQALADR LGVKLKLQPT NPANRIPFLT SGKVDLVLAN FTITDERAKQ VDFSVPYFAS GQQFLAKKGV LKSADQLNRL RIGADKGTTN EVTLREKYPQ ATIVAYDDTP FAFAALRAGN VQAITQDGPK LIGLLANVPD KQNYEVPAFS ISNDYMGVGA PKGEPRLVAF VNETLTDLEA SGRAGRIYDA WFGPNTKTPL ARIFKIG
Validated NT Sequence
tcgccgatct tgaagatccg cgcgagcggc gtcttcgtat tcggcccgaa ccatgcgtcg tagatccggc ccgcgcggcc gctcgcctcg agatccgtca gcgtttcgtt gacgaacgcg acgagccgcg gctcgccctt cggcgcgccg acgcccatgt aatcgttcga gatcgagaac gccggcactt cgtagttctg cttgtccggc acgttcgcga gcaggccgat cagcttcggg ccgtcctgcg tgatcgcctg cacgttgccc gcgcgcagcg cggcgaatgc gaacggcgtg tcgtcgtagg cgacgatcgt cgcctgcgga tatttctcgc gcagcgtgac ctcgttcgtc gtacccttgt cggcgccgat gcgcaggcgg ttcaactgat cggccgattt cagcacgccc ttcttcgcga gaaactgctg ccccgacgcg aaatacggca cgctgaagtc gacctgcttc gcacgctcgt cggtgatcgt gaagttcgcg agcacgagat cgaccttgcc cgaggtcagg aacggaatgc ggttcgcggg attggtcggc tgcagcttca gcttgacgcc gagccggtcg gcgagcgcct gcgcatagtc gacgtcgagg ccgacgatcc ggttcgtcct cggatcgacg aagccgaacg gcgggttgct gtcgaacgtc gcgacgcgca gcacgccggc cttccggata tcgtcgagac gatccgccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADRLDDIR KAGVLRVATF DSNPPFGFVD PRTNRIVGLD VDYAQALADR LGVKLKLQPT NPANRIPFLT SGKVDLVLAN FTITDERAKQ VDFSVPYFAS GQQFLAKKGV LKSADQLNRL RIGADKGTTN EVTLREKYPQ ATIVAYDDTP FAFAALRAGN VQAITQDGPK LIGLLANVPD KQNYEVPAFS ISNDYMGVGA PKGEPRLVAF VNETLTDLEA SGRAGRIYDA WFGPNTKTPL ARIFKIGDKR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggatcgt ctcgacgata tccggaaggc cggcgtgctg cgcgtcgcga cgttcgacag caacccgccg ttcggcttcg tcgatccgag gacgaaccgg atcgtcggcc tcgacgtcga ctatgcgcag gcgctcgccg accggctcgg cgtcaagctg aagctgcagc cgaccaatcc cgcgaaccgc attccgttcc tgacctcggg caaggtcgat ctcgtgctcg cgaacttcac gatcaccgac gagcgtgcga agcaggtcga cttcagcgtg ccgtatttcg cgtcggggca gcagtttctc gcgaagaagg gcgtgctgaa atcggccgat cagttgaacc gcctgcgcat cggcgccgac aagggtacga cgaacgaggt cacgctgcgc gagaaatatc cgcaggcgac gatcgtcgcc tacgacgaca cgccgttcgc attcgccgcg ctgcgcgcgg gcaacgtgca ggcgatcacg caggacggcc cgaagctgat cggcctgctc gcgaacgtgc cggacaagca gaactacgaa gtgccggcgt tctcgatctc gaacgattac atgggcgtcg gcgcgccgaa gggcgagccg cggctcgtcg cgttcgtcaa cgaaacgctg acggatctcg aggcgagcgg ccgcgcgggc cggatctacg acgcatggtt cgggccgaat acgaagacgc cgctcgcgcg gatcttcaag atcggcgaca agcgctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsA.17285.e.A2.PS01145
PURIFICATION DATe: 7/6/2011
CONCENTRATION: 31.68mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADRLDDIR KAGVLRVATF DSNPPFGFVD PRTNRIVGLD VDYAQALADR LGVKLKLQPT NPANRIPFLT SGKVDLVLAN FTITDERAKQ VDFSVPYFAS GQQFLAKKGV LKSADQLNRL RIGADKGTTN EVTLREKYPQ ATIVAYDDTP FAFAALRAGN VQAITQDGPK LIGLLANVPD KQNYEVPAFS ISNDYMGVGA PKGEPRLVAF VNETLTDLEA SGRAGRIYDA WFGPNTKTPL ARIFKIG
Validated NT Sequence
tcgccgatct tgaagatccg cgcgagcggc gtcttcgtat tcggcccgaa ccatgcgtcg tagatccggc ccgcgcggcc gctcgcctcg agatccgtca gcgtttcgtt gacgaacgcg acgagccgcg gctcgccctt cggcgcgccg acgcccatgt aatcgttcga gatcgagaac gccggcactt cgtagttctg cttgtccggc acgttcgcga gcaggccgat cagcttcggg ccgtcctgcg tgatcgcctg cacgttgccc gcgcgcagcg cggcgaatgc gaacggcgtg tcgtcgtagg cgacgatcgt cgcctgcgga tatttctcgc gcagcgtgac ctcgttcgtc gtacccttgt cggcgccgat gcgcaggcgg ttcaactgat cggccgattt cagcacgccc ttcttcgcga gaaactgctg ccccgacgcg aaatacggca cgctgaagtc gacctgcttc gcacgctcgt cggtgatcgt gaagttcgcg agcacgagat cgaccttgcc cgaggtcagg aacggaatgc ggttcgcggg attggtcggc tgcagcttca gcttgacgcc gagccggtcg gcgagcgcct gcgcatagtc gacgtcgagg ccgacgatcc ggttcgtcct cggatcgacg aagccgaacg gcgggttgct gtcgaacgtc gcgacgcgca gcacgccggc cttccggata tcgtcgagac gatccgccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADRLDDIR KAGVLRVATF DSNPPFGFVD PRTNRIVGLD VDYAQALADR LGVKLKLQPT NPANRIPFLT SGKVDLVLAN FTITDERAKQ VDFSVPYFAS GQQFLAKKGV LKSADQLNRL RIGADKGTTN EVTLREKYPQ ATIVAYDDTP FAFAALRAGN VQAITQDGPK LIGLLANVPD KQNYEVPAFS ISNDYMGVGA PKGEPRLVAF VNETLTDLEA SGRAGRIYDA WFGPNTKTPL ARIFKIGDKR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGATCGT CTCGACGATA TCCGGAAGGC CGGCGTGCTG CGCGTCGCGA CGTTCGACAG CAACCCGCCG TTCGGCTTCG TCGATCCGAG GACGAACCGG ATCGTCGGCC TCGACGTCGA CTATGCGCAG GCGCTCGCCG ACCGGCTCGG CGTCAAGCTG AAGCTGCAGC CGACCAATCC CGCGAACCGC ATTCCGTTCC TGACCTCGGG CAAGGTCGAT CTCGTGCTCG CGAACTTCAC GATCACCGAC GAGCGTGCGA AGCAGGTCGA CTTCAGCGTG CCGTATTTCG CGTCGGGGCA GCAGTTTCTC GCGAAGAAGG GCGTGCTGAA ATCGGCCGAT CAGTTGAACC GCCTGCGCAT CGGCGCCGAC AAGGGTACGA CGAACGAGGT CACGCTGCGC GAGAAATATC CGCAGGCGAC GATCGTCGCC TACGACGACA CGCCGTTCGC ATTCGCCGCG CTGCGCGCGG GCAACGTGCA GGCGATCACG CAGGACGGCC CGAAGCTGAT CGGCCTGCTC GCGAACGTGC CGGACAAGCA GAACTACGAA GTGCCGGCGT TCTCGATCTC GAACGATTAC ATGGGCGTCG GCGCGCCGAA GGGCGAGCCG CGGCTCGTCG CGTTCGTCAA CGAAACGCTG ACGGATCTCG AGGCGAGCGG CCGCGCGGGC CGGATCTACG ACGCATGGTT CGGGCCGAAT ACGAAGACGC CGCTCGCGCG GATCTTCAAG ATCGGCGACA AGCGCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA
Details for BupsA.17285.e.A2.PS01146
PURIFICATION DATe: 7/6/2011
CONCENTRATION: 67.55mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADRLDDIR KAGVLRVATF DSNPPFGFVD PRTNRIVGLD VDYAQALADR LGVKLKLQPT NPANRIPFLT SGKVDLVLAN FTITDERAKQ VDFSVPYFAS GQQFLAKKGV LKSADQLNRL RIGADKGTTN EVTLREKYPQ ATIVAYDDTP FAFAALRAGN VQAITQDGPK LIGLLANVPD KQNYEVPAFS ISNDYMGVGA PKGEPRLVAF VNETLTDLEA SGRAGRIYDA WFGPNTKTPL ARIFKIG
Validated NT Sequence
tcgccgatct tgaagatccg cgcgagcggc gtcttcgtat tcggcccgaa ccatgcgtcg tagatccggc ccgcgcggcc gctcgcctcg agatccgtca gcgtttcgtt gacgaacgcg acgagccgcg gctcgccctt cggcgcgccg acgcccatgt aatcgttcga gatcgagaac gccggcactt cgtagttctg cttgtccggc acgttcgcga gcaggccgat cagcttcggg ccgtcctgcg tgatcgcctg cacgttgccc gcgcgcagcg cggcgaatgc gaacggcgtg tcgtcgtagg cgacgatcgt cgcctgcgga tatttctcgc gcagcgtgac ctcgttcgtc gtacccttgt cggcgccgat gcgcaggcgg ttcaactgat cggccgattt cagcacgccc ttcttcgcga gaaactgctg ccccgacgcg aaatacggca cgctgaagtc gacctgcttc gcacgctcgt cggtgatcgt gaagttcgcg agcacgagat cgaccttgcc cgaggtcagg aacggaatgc ggttcgcggg attggtcggc tgcagcttca gcttgacgcc gagccggtcg gcgagcgcct gcgcatagtc gacgtcgagg ccgacgatcc ggttcgtcct cggatcgacg aagccgaacg gcgggttgct gtcgaacgtc gcgacgcgca gcacgccggc cttccggata tcgtcgagac gatccgccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADRLDDIR KAGVLRVATF DSNPPFGFVD PRTNRIVGLD VDYAQALADR LGVKLKLQPT NPANRIPFLT SGKVDLVLAN FTITDERAKQ VDFSVPYFAS GQQFLAKKGV LKSADQLNRL RIGADKGTTN EVTLREKYPQ ATIVAYDDTP FAFAALRAGN VQAITQDGPK LIGLLANVPD KQNYEVPAFS ISNDYMGVGA PKGEPRLVAF VNETLTDLEA SGRAGRIYDA WFGPNTKTPL ARIFKIGDKR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGATCGT CTCGACGATA TCCGGAAGGC CGGCGTGCTG CGCGTCGCGA CGTTCGACAG CAACCCGCCG TTCGGCTTCG TCGATCCGAG GACGAACCGG ATCGTCGGCC TCGACGTCGA CTATGCGCAG GCGCTCGCCG ACCGGCTCGG CGTCAAGCTG AAGCTGCAGC CGACCAATCC CGCGAACCGC ATTCCGTTCC TGACCTCGGG CAAGGTCGAT CTCGTGCTCG CGAACTTCAC GATCACCGAC GAGCGTGCGA AGCAGGTCGA CTTCAGCGTG CCGTATTTCG CGTCGGGGCA GCAGTTTCTC GCGAAGAAGG GCGTGCTGAA ATCGGCCGAT CAGTTGAACC GCCTGCGCAT CGGCGCCGAC AAGGGTACGA CGAACGAGGT CACGCTGCGC GAGAAATATC CGCAGGCGAC GATCGTCGCC TACGACGACA CGCCGTTCGC ATTCGCCGCG CTGCGCGCGG GCAACGTGCA GGCGATCACG CAGGACGGCC CGAAGCTGAT CGGCCTGCTC GCGAACGTGC CGGACAAGCA GAACTACGAA GTGCCGGCGT TCTCGATCTC GAACGATTAC ATGGGCGTCG GCGCGCCGAA GGGCGAGCCG CGGCTCGTCG CGTTCGTCAA CGAAACGCTG ACGGATCTCG AGGCGAGCGG CCGCGCGGGC CGGATCTACG ACGCATGGTT CGGGCCGAAT ACGAAGACGC CGCTCGCGCG GATCTTCAAG ATCGGCGACA AGCGCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA