SSGCID: Target Page

BupsA.17285.d

Glutamate/aspartate periplasmic binding protein

CENTER ID:	BupsA.17285.d
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17285.d.A2.GE32558	Mature protein( BupsA.17285.d )		22	297

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17285.d.A2.PS01153	Mature protein( BupsA.17285.d )		22	297

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.3576
RefSeq:	YP_334805.1
UniProt:	Q3JNP8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKFPKAMLMV AALSTFAGGA IAQETGTLKK IKDTGVIALG HRESSIPFSY YDQNQQVVGY SRDFQMKVVD AVKKKLNLPN LQVKNIPVTS QNRIPLVQNG TVDIECGSTT NNLDRQKQAA FSDTIFVIGT RLMTKKDSGI KDFADLKGKT VVTTAGTTSE RLLREMNNKN QMGMSIISAK DHGESFQTLE TGRAVAFMMD DALLAGERAK AKQPGEWVIV GKPQSQEAYG CMMRKDDPAF KKVVDDAIVQ VEKSGEAAKI YSKWFENPIP PKGLNLNFPL SDEMKKLYAN PNDKALD

NT Sequence

ATGAAATTCC CGAAAGCAAT GCTGATGGTC GCGGCGCTGA GCACTTTTGC CGGCGGCGCC ATCGCCCAGG AAACGGGCAC GCTGAAAAAA ATCAAGGACA CGGGCGTGAT TGCGCTGGGG CACCGCGAAT CGTCGATTCC GTTCTCGTAC TATGACCAGA ATCAGCAGGT CGTCGGCTAT TCGCGCGACT TCCAGATGAA AGTCGTCGAC GCGGTCAAGA AGAAGCTGAA TCTGCCCAAC CTGCAGGTGA AGAATATTCC GGTGACGTCG CAGAACCGGA TTCCGCTCGT GCAGAACGGC ACGGTCGACA TCGAGTGCGG CTCGACCACG AACAACCTCG ATCGCCAGAA GCAGGCGGCG TTCTCCGACA CGATCTTCGT GATCGGCACG CGCCTCATGA CGAAGAAGGA TTCGGGCATC AAGGATTTCG CCGATCTGAA GGGCAAGACC GTCGTCACGA CGGCGGGCAC GACGTCCGAG CGCCTGCTGC GCGAGATGAA CAACAAGAAC CAGATGGGCA TGAGCATCAT CAGCGCGAAG GACCACGGCG AGTCGTTCCA GACGCTCGAG ACGGGCCGCG CGGTCGCGTT CATGATGGAC GACGCGCTGC TCGCGGGCGA GCGCGCGAAG GCGAAGCAGC CGGGCGAGTG GGTGATCGTC GGCAAGCCGC AGTCTCAGGA AGCGTACGGC TGCATGATGC GCAAGGACGA TCCGGCGTTC AAGAAGGTGG TCGACGACGC GATCGTGCAG GTCGAGAAGT CGGGCGAAGC CGCGAAGATC TATTCGAAGT GGTTCGAGAA TCCGATTCCG CCGAAGGGCC TGAATCTGAA CTTCCCGCTC TCCGACGAGA TGAAGAAGCT CTACGCGAAC CCGAACGACA AGGCGCTCGA C

Details for BupsA.17285.d.A2.GE32558

HARVESTED ON:	5/17/2011
SEQUENCED ON:	5/23/2011
EXPECTED MW:	32kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAQETGTLK KIKDTGVIAL GHRESSIPFS YYDQNQQVVG YSRDFQMKVV DAVKKKLNLP NLQVKNIPVT SQNRIPLVQN GTVDIECGST TNNLDRQKQA AFSDTIFVIG TRLMTKKDSG IKDFADLKGK TVVTTAGTTS ERLLREMNNK NQMGMSIISA KDHGESFQTL ETGRAVAFMM DDALLAGERA KAKQPGEWVI VGKPQSQEAY GCMMRKDDPA FKKVVDDAIV QVEKSGEAAK IYSKWFENPI PPKGLNLNFP LSDEMKKLYA NPNDKALD

Validated NT Sequence

ttattagtcg agcgccttgt cgttcgggtt cgcgtagagc ttcttcatct cgtcggagag cgggaagttc agattcaggc ccttcggcgg aatcggattc tcgaaccact tcgaatagat cttcgcggct tcgcccgact tctcgacctg cacgatcgcg tcgtcgacca ccttcttgaa cgccggatcg tccttgcgca tcatgcagcc gtacgcttcc tgagactgcg gcttgccgac gatcacccac tcgcccggct gcttcgcctt cgcgcgctcg cccgcgagca gcgcgtcgtc catcatgaac gcgaccgcgc ggcccgtctc gagcgtctgg aacgactcgc cgtggtcctt cgcgctgatg atgctcatgc ccatctggtt cttgttgttc atctcgcgca gcaggcgctc ggacgtcgtg cccgccgtcg tgacgacggt cttgcccttc agatcggcga aatccttgat gcccgaatcc ttcttcgtca tgaggcgcgt gccgatcacg aagatcgtgt cggagaacgc cgcctgcttc tggcgatcga ggttgttcgt ggtcgagccg cactcgatgt cgaccgtgcc gttctgcacg agcggaatcc ggttctgcga cgtcaccgga atattcttca cctgcaggtt gggcagattc agcttcttct tgaccgcgtc gacgactttc atctggaagt cgcgcgaata gccgacgacc tgctgattct ggtcatagta cgagaacgga atcgacgatt cgcggtgccc cagcgcaatc acgcccgtgt ccttgatttt tttcagcgtg cccgtttcct gggccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat ggtatatc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcccaggaa acgggcacgc tgaaaaaaat caaggacacg ggcgtgattg cgctggggca ccgcgaatcg tcgattccgt tctcgtacta tgaccagaat cagcaggtcg tcggctattc gcgcgacttc cagatgaaag tcgtcgacgc ggtcaagaag aagctgaatc tgcccaacct gcaggtgaag aatattccgg tgacgtcgca gaaccggatt ccgctcgtgc agaacggcac ggtcgacatc gagtgcggct cgaccacgaa caacctcgat cgccagaagc aggcggcgtt ctccgacacg atcttcgtga tcggcacgcg cctcatgacg aagaaggatt cgggcatcaa ggatttcgcc gatctgaagg gcaagaccgt cgtcacgacg gcgggcacga cgtccgagcg cctgctgcgc gagatgaaca acaagaacca gatgggcatg agcatcatca gcgcgaagga ccacggcgag tcgttccaga cgctcgagac gggccgcgcg gtcgcgttca tgatggacga cgcgctgctc gcgggcgagc gcgcgaaggc gaagcagccg ggcgagtggg tgatcgtcgg caagccgcag tctcaggaag cgtacggctg catgatgcgc aaggacgatc cggcgttcaa gaaggtggtc gacgacgcga tcgtgcaggt cgagaagtcg ggcgaagccg cgaagatcta ttcgaagtgg ttcgagaatc cgattccgcc gaagggcctg aatctgaact tcccgctctc cgacgagatg aagaagctct acgcgaaccc gaacgacaag gcgctcgact aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for BupsA.17285.d.A2.PS01153

PURIFICATION DATe:	7/12/2011
CONCENTRATION:	79.55mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCCAGGAA ACGGGCACGC TGAAAAAAAT CAAGGACACG GGCGTGATTG CGCTGGGGCA CCGCGAATCG TCGATTCCGT TCTCGTACTA TGACCAGAAT CAGCAGGTCG TCGGCTATTC GCGCGACTTC CAGATGAAAG TCGTCGACGC GGTCAAGAAG AAGCTGAATC TGCCCAACCT GCAGGTGAAG AATATTCCGG TGACGTCGCA GAACCGGATT CCGCTCGTGC AGAACGGCAC GGTCGACATC GAGTGCGGCT CGACCACGAA CAACCTCGAT CGCCAGAAGC AGGCGGCGTT CTCCGACACG ATCTTCGTGA TCGGCACGCG CCTCATGACG AAGAAGGATT CGGGCATCAA GGATTTCGCC GATCTGAAGG GCAAGACCGT CGTCACGACG GCGGGCACGA CGTCCGAGCG CCTGCTGCGC GAGATGAACA ACAAGAACCA GATGGGCATG AGCATCATCA GCGCGAAGGA CCACGGCGAG TCGTTCCAGA CGCTCGAGAC GGGCCGCGCG GTCGCGTTCA TGATGGACGA CGCGCTGCTC GCGGGCGAGC GCGCGAAGGC GAAGCAGCCG GGCGAGTGGG TGATCGTCGG CAAGCCGCAG TCTCAGGAAG CGTACGGCTG CATGATGCGC AAGGACGATC CGGCGTTCAA GAAGGTGGTC GACGACGCGA TCGTGCAGGT CGAGAAGTCG GGCGAAGCCG CGAAGATCTA TTCGAAGTGG TTCGAGAATC CGATTCCGCC GAAGGGCCTG AATCTGAACT TCCCGCTCTC CGACGAGATG AAGAAGCTCT ACGCGAACCC GAACGACAAG GCGCTCGACT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA