BupsA.17285.b
Glutamine-binding periplasmic protein
| CENTER ID: | BupsA.17285.b |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.17285.b.A2.GE32557 | Mature protein( BupsA.17285.b ) | 25 | 250 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.17285.b.A2.PS01148 | Mature protein( BupsA.17285.b ) | 25 | 250 |
Structures
4F3P
DEPOSITED: 5/9/2012
DETERMINATION: XRay
CLONE: BupsA.17285.b.A2.GE32557
PROTEIN: BupsA.17285.b.A2.PS01148
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127777 |
| PATRIC ID: | fig|320372.6.peg.5595 |
| RefSeq: | YP_336644.1 |
| UniProt: | Q3JIF9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGRRSFIKTI VALALVGASA AGAHAKELVV GTDTSFMPFE FKQGDKYVGF DLDLWAEIAK GAGWTYKIQP MDFAGLIPAL QTQNIDVALS GMTIKEERRK AIDFSDPYYD SGLAAMVQAN NTTIKSIDDL NGKVIAAKTG TATIDWIKAH LKPKEIRQFP NIDQAYLALE AGRVDAAMHD TPNVLFFVNN EGKGRVKVAG APVSGDKYGI GFPKGSPLVA KVNAELARMK ADGRYAKIYK KWFGSEPPKS
NT Sequence
ATGGGACGCC GTTCGTTCAT CAAGACAATC GTCGCGCTCG CGCTCGTCGG CGCGAGCGCC GCGGGCGCGC ACGCGAAGGA GCTCGTCGTC GGCACCGATA CTTCGTTCAT GCCGTTCGAG TTCAAGCAGG GCGACAAGTA CGTCGGCTTC GACCTCGATC TGTGGGCCGA GATCGCGAAG GGCGCCGGCT GGACCTACAA GATCCAGCCG ATGGATTTCG CCGGCCTGAT TCCGGCGCTG CAGACGCAGA ACATCGACGT CGCGCTGTCC GGGATGACGA TCAAGGAGGA GCGCAGGAAG GCGATCGATT TCTCCGATCC ATATTACGAC AGCGGCCTCG CCGCGATGGT CCAGGCGAAC AACACGACGA TCAAGTCGAT CGACGATCTG AACGGCAAGG TGATCGCCGC GAAGACAGGC ACCGCGACGA TCGACTGGAT CAAGGCGCAT CTGAAGCCGA AGGAGATCCG CCAGTTCCCG AACATCGACC AGGCGTATCT CGCGCTGGAG GCGGGCCGCG TCGACGCCGC GATGCACGAC ACGCCGAACG TGCTGTTCTT CGTGAACAAC GAGGGCAAGG GGCGCGTGAA GGTGGCGGGC GCGCCGGTGA GCGGCGACAA GTACGGCATC GGCTTCCCGA AGGGCAGCCC GCTCGTCGCG AAGGTCAACG CGGAGCTCGC GAGGATGAAG GCCGACGGCC GCTACGCGAA GATCTACAAG AAGTGGTTCG GCTCCGAGCC GCCGAAGTCT
Details for BupsA.17285.b.A2.GE32557
| HARVESTED ON: | 5/17/2011 |
| SEQUENCED ON: | 5/23/2011 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Validated AA Sequence
MGTLEAQTQG PGSMAKELVV GTDTSFMPFE FKQGDKYVGF DLDLWAEIAK GAGWTYKIQP MDFAGLIPAL QTQNIDVALS GMTIKEERRK AIDFSDPYYD SGLAAMVQAN NTTIKSIDDL NGKVIAAKTG TATIDWIKAH LKPKEIRQFP NIDQAYLALE AGRVDAAMHD TPNVLFFVNN EGKGRVKVAG APVSGDKYGI GFPKGSPLVA KVNAELARMK ADGRYAKIYK KWFGSEPP
Validated NT Sequence
tcggcggctc ggagccgaac cacttcttgt agatcttcgc gtagcggccg tcggccttca tcctcgcgag ctccgcgttg accttcgcga cgagcgggct gcccttcggg aagccgatgc cgtacttgtc gccgctcacc ggcgcgcccg ccaccttcac gcgccccttg ccctcgttgt tcacgaagaa cagcacgttc ggcgtgtcgt gcatcgcggc gtcgacgcgg cccgcctcca gcgcgagata cgcctggtcg atgttcggga actggcggat ctccttcggc ttcagatgcg ccttgatcca gtcgatcgtc gcggtgcctg tcttcgcggc gatcaccttg ccgttcagat cgtcgatcga cttgatcgtc gtgttgttcg cctggaccat cgcggcgagg ccgctgtcgt aatatggatc ggagaaatcg atcgccttcc tgcgctcctc cttgatcgtc atcccggaca gcgcgacgtc gatgttctgc gtctgcagcg ccggaatcag gccggcgaaa tccatcggct ggatcttgta ggtccagccg gcgcccttcg cgatctcggc ccacagatcg aggtcgaagc cgacgtactt gtcgccctgc ttgaactcga acggcatgaa cgaagtatcg gtgccgacga cgagctcctt cgccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKELVVGT DTSFMPFEFK QGDKYVGFDL DLWAEIAKGA GWTYKIQPMD FAGLIPALQT QNIDVALSGM TIKEERRKAI DFSDPYYDSG LAAMVQANNT TIKSIDDLNG KVIAAKTGTA TIDWIKAHLK PKEIRQFPNI DQAYLALEAG RVDAAMHDTP NVLFFVNNEG KGRVKVAGAP VSGDKYGIGF PKGSPLVAKV NAELARMKAD GRYAKIYKKW FGSEPPKS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgaaggag ctcgtcgtcg gcaccgatac ttcgttcatg ccgttcgagt tcaagcaggg cgacaagtac gtcggcttcg acctcgatct gtgggccgag atcgcgaagg gcgccggctg gacctacaag atccagccga tggatttcgc cggcctgatt ccggcgctgc agacgcagaa catcgacgtc gcgctgtccg ggatgacgat caaggaggag cgcaggaagg cgatcgattt ctccgatcca tattacgaca gcggcctcgc cgcgatggtc caggcgaaca acacgacgat caagtcgatc gacgatctga acggcaaggt gatcgccgcg aagacaggca ccgcgacgat cgactggatc aaggcgcatc tgaagccgaa ggagatccgc cagttcccga acatcgacca ggcgtatctc gcgctggagg cgggccgcgt cgacgccgcg atgcacgaca cgccgaacgt gctgttcttc gtgaacaacg agggcaaggg gcgcgtgaag gtggcgggcg cgccggtgag cggcgacaag tacggcatcg gcttcccgaa gggcagcccg ctcgtcgcga aggtcaacgc ggagctcgcg aggatgaagg ccgacggccg ctacgcgaag atctacaaga agtggttcgg ctccgagccg ccgaagtctt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BupsA.17285.b.A2.PS01148
| PURIFICATION DATe: | 7/11/2011 |
| CONCENTRATION: | 56.11mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 95 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMAKELVV GTDTSFMPFE FKQGDKYVGF DLDLWAEIAK GAGWTYKIQP MDFAGLIPAL QTQNIDVALS GMTIKEERRK AIDFSDPYYD SGLAAMVQAN NTTIKSIDDL NGKVIAAKTG TATIDWIKAH LKPKEIRQFP NIDQAYLALE AGRVDAAMHD TPNVLFFVNN EGKGRVKVAG APVSGDKYGI GFPKGSPLVA KVNAELARMK ADGRYAKIYK KWFGSEPP
Validated NT Sequence
tcggcggctc ggagccgaac cacttcttgt agatcttcgc gtagcggccg tcggccttca tcctcgcgag ctccgcgttg accttcgcga cgagcgggct gcccttcggg aagccgatgc cgtacttgtc gccgctcacc ggcgcgcccg ccaccttcac gcgccccttg ccctcgttgt tcacgaagaa cagcacgttc ggcgtgtcgt gcatcgcggc gtcgacgcgg cccgcctcca gcgcgagata cgcctggtcg atgttcggga actggcggat ctccttcggc ttcagatgcg ccttgatcca gtcgatcgtc gcggtgcctg tcttcgcggc gatcaccttg ccgttcagat cgtcgatcga cttgatcgtc gtgttgttcg cctggaccat cgcggcgagg ccgctgtcgt aatatggatc ggagaaatcg atcgccttcc tgcgctcctc cttgatcgtc atcccggaca gcgcgacgtc gatgttctgc gtctgcagcg ccggaatcag gccggcgaaa tccatcggct ggatcttgta ggtccagccg gcgcccttcg cgatctcggc ccacagatcg aggtcgaagc cgacgtactt gtcgccctgc ttgaactcga acggcatgaa cgaagtatcg gtgccgacga cgagctcctt cgccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAKELVVGT DTSFMPFEFK QGDKYVGFDL DLWAEIAKGA GWTYKIQPMD FAGLIPALQT QNIDVALSGM TIKEERRKAI DFSDPYYDSG LAAMVQANNT TIKSIDDLNG KVIAAKTGTA TIDWIKAHLK PKEIRQFPNI DQAYLALEAG RVDAAMHDTP NVLFFVNNEG KGRVKVAGAP VSGDKYGIGF PKGSPLVAKV NAELARMKAD GRYAKIYKKW FGSEPPKS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGAAGGAG CTCGTCGTCG GCACCGATAC TTCGTTCATG CCGTTCGAGT TCAAGCAGGG CGACAAGTAC GTCGGCTTCG ACCTCGATCT GTGGGCCGAG ATCGCGAAGG GCGCCGGCTG GACCTACAAG ATCCAGCCGA TGGATTTCGC CGGCCTGATT CCGGCGCTGC AGACGCAGAA CATCGACGTC GCGCTGTCCG GGATGACGAT CAAGGAGGAG CGCAGGAAGG CGATCGATTT CTCCGATCCA TATTACGACA GCGGCCTCGC CGCGATGGTC CAGGCGAACA ACACGACGAT CAAGTCGATC GACGATCTGA ACGGCAAGGT GATCGCCGCG AAGACAGGCA CCGCGACGAT CGACTGGATC AAGGCGCATC TGAAGCCGAA GGAGATCCGC CAGTTCCCGA ACATCGACCA GGCGTATCTC GCGCTGGAGG CGGGCCGCGT CGACGCCGCG ATGCACGACA CGCCGAACGT GCTGTTCTTC GTGAACAACG AGGGCAAGGG GCGCGTGAAG GTGGCGGGCG CGCCGGTGAG CGGCGACAAG TACGGCATCG GCTTCCCGAA GGGCAGCCCG CTCGTCGCGA AGGTCAACGC GGAGCTCGCG AGGATGAAGG CCGACGGCCG CTACGCGAAG ATCTACAAGA AGTGGTTCGG CTCCGAGCCG CCGAAGTCTT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA