SSGCID: Target Page

BupsA.17254.a

virulence factor BPSL1958 ortholog

CENTER ID:	BupsA.17254.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17254.a.A1.GE30013	full length		1	201

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.1959
RefSeq:	YP_333273.1
UniProt:	Q3JT30

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKLAASNQF GIPNQTDVFA VDGNGSLRVS WVVSAGAWNG PAQIGPAGLF PSRAAVASSN QFGIPNQTDV FAVGRDGALN VAWVVSADRW NGPTPISAAG LFPAGAAIAA SNQFGIPNQT DVFAVSDSGA LNVAWVVSAE RWNGPIPISA AGHFPAGAPL ATSNQFGIPN QTDVFAADSD GVLHVAWVVS AGNWNGPISI A

NT Sequence

atggcaaaat tagcagcttc aaatcaattc ggcattccca atcagaccga cgtatttgcc gttgacggca acggctcgct gcgcgtttcc tgggtggtca gcgccggcgc ctggaacggc cccgctcaga tcgggccggc ggggctcttc ccgtcccgcg cggcggttgc gtcgtcgaac caattcggca tcccgaatca gaccgacgtg ttcgccgtcg gccgcgacgg cgcgctgaac gtcgcgtggg tggtcagcgc cgatcgctgg aacggaccga ccccgatcag cgccgcgggc cttttcccgg ccggcgcggc gatcgcggcg tcgaaccagt tcggcatccc gaatcagacc gacgtgtttg ccgtgagcga cagcggcgcg ttgaacgtgg cgtgggtcgt cagcgccgag cgctggaacg gaccgattcc gatcagcgcg gcgggccact tcccggcggg cgcgccgctc gcgacatcga accagttcgg catcccgaat cagaccgacg tgttcgccgc cgatagcgac ggcgtcctac acgtcgcgtg ggtggtcagc gccggcaatt ggaacgggcc gatttccatt gcgtga

Details for BupsA.17254.a.A1.GE30013

HARVESTED ON:	8/10/2010
SEQUENCED ON:	8/16/2010
EXPECTED MW:	22kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAKLAASNQ FGIPNQTDVF AVDGNGSLRV SWVVSAGAWN GPAQIGPAGL FPSRAAVASS NQFGIPNQTD VFAVGRDGAL NVAWVVSADR WNGPTPISAA GLFPAGAAIA ASNQFGIPNQ TDVFAVSDSG ALNVAWVVSA ERWNGPIPIS AAGHFPAGAP LATSNQFGIP NQTDVFAADS DGVLHVAWVV SAGNWNGPIS IA

Validated NT Sequence

cnnnnnnnna ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcaaa attagcagct tcaaatcaat tcggcattcc caatcagacc gacgtatttg ccgttgacgg caacggctcg ctgcgcgttt cctgggtggt cagcgccggc gcctggaacg gccccgctca gatcgggccg gcggggctct tcccgtcccg cgcggcggtt gcgtcgtcga accaattcgg catcccgaat cagaccgacg tgttcgccgt cggccgcgac ggcgcgctga acgtcgcgtg ggtggtcagc gccgatcgct ggaacggacc gaccccgatc agcgccgcgg gccttttccc ggccggcgcg gcgatcgcgg cgtcgaacca gttcggcatc ccgaatcaga ccgacgtgtt tgccgtgagc gacagcggcg cgttgaacgt ggcgtgggtc gtcagcgccg agcgctggaa cggaccgatt ccgatcagcg cggcgggcca cttcccggcg ggcgcgccgc tcgcgacatc gaaccagttc ggcatcccga atcagaccga cgtgttcgcc gccgatagcg acggcgtcct acacgtcgcg tgggtggtca gcgccggcaa ttggaacggg ccgatttcca ttgcgtaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acanagcccg aaaggaagct gagttggctg ctgccannnn nnnnnnnnnn nannnnncnn ccnnnncngt cggccncnna angaccctgc tcncccancg nggntnnngn cnnnnanncn ncncnnnnnn cnnnnnnnnn nntnnnnncc atcnncgncn nnancccnnc tnncnngann nngnnnnnng cnnnacnaan nnnncnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaaaatta gcagcttcaa atcaattcgg cattcccaat cagaccgacg tatttgccgt tgacggcaac ggctcgctgc gcgtttcctg ggtggtcagc gccggcgcct ggaacggccc cgctcagatc gggccggcgg ggctcttccc gtcccgcgcg gcggttgcgt cgtcgaacca attcggcatc ccgaatcaga ccgacgtgtt cgccgtcggc cgcgacggcg cgctgaacgt cgcgtgggtg gtcagcgccg atcgctggaa cggaccgacc ccgatcagcg ccgcgggcct tttcccggcc ggcgcggcga tcgcggcgtc gaaccagttc ggcatcccga atcagaccga cgtgtttgcc gtgagcgaca gcggcgcgtt gaacgtggcg tgggtcgtca gcgccgagcg ctggaacgga ccgattccga tcagcgcggc gggccacttc ccggcgggcg cgccgctcgc gacatcgaac cagttcggca tcccgaatca gaccgacgtg ttcgccgccg atagcgacgg cgtcctacac gtcgcgtggg tggtcagcgc cggcaattgg aacgggccga tttccattgc gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa