BupsA.17253.b

Phospholipase C (3.1.4.3)

CENTER ID:	BupsA.17253.b
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17253.b.A1.GE34756	full length		1	749
BupsA.17253.b.A2.GE34754	mature protein		52	749

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17253.b.A1.PS01461	full length		1	749
BupsA.17253.b.A1.PS01462	full length		1	749

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_215259
PATRIC ID:	fig\|320372.6.peg.5697
RefSeq:	YP_336734.1
UniProt:	Q3JI69

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRASPVHRSV NPYQGLRNMN ARRRFLQGTA TTGIAAATLA AFPLSIRRAL AIPANNRTGT IRDVEHIVIL MQENRSFDNY FGTLRGVRGF GDRFGIPLPN ALTVWQQRNA TGALVLPYHL DGSKGNAQRV SGTPHSWDDG QNAWDGGRMY QWPRYKNTAS MGYFRESELP FQFALANAFT ICDAYHCSMH AGTNSNRMFM WTGTNGPTGA GVASVVNEWD DIGPSTFGYE WKTYPERLQE AGVSWKVYQN MPDNFTDNAL AGFRQYRRAN EASGKPVSNS DKVVSPAYDP ASDDVRNPLY KGIANTMPDG GFLGAFKEDI RAGKLPQVSW VVAPAAYSEH PGPSSPVQGA WYIQEVLDAL TATPDVWSKT VLLVNFDEND GYFDHVPSPS APSLNPDGSP AGKTTLPEAD IAFERFTHPK PPGTKSQPQP DARVYGPGVR VPMYVISPWS RGGWVNSQVF DHTSTLRFIE ARFGVREPNI SAFRRAVCGD LTSAFNFVRP NNEPLPTLAG RKTRAQADAL RAAQQQMPQI VPPANGLLPR QETGTRPSRA LPYELHVNAV ARTLDGTLRL VFANTGQAAA VFHVYDKLNL GRLPRRYMVE AGKQLDDVWA AMTDNGGKYD LWVLGPNGLH RHFTGDMNRL RAGGAPVPEV RVEYDRQHGN VQLLLRNEGA RECVFTVRAK AYRDDGPWTF KVKGGAERKH HWKLDGSGNW YDFVVTCDAD TGYSRRFAGR VETGEHSVSD PAMGFGDRF

NT Sequence

atgcgcgcat cgccggttca tcgctcggtc aacccatacc aaggactccg gaacatgaac gctcgtcgca gatttctgca aggcaccgca accaccggca tcgcggccgc cacgttggcc gcgtttccgc tcagcattcg ccgcgcactc gcgattcccg ccaacaaccg caccggaacc atccgggatg tcgagcacat cgtcatcctg atgcaggaaa accgctcgtt cgacaactac ttcggcacgc tgcgcggcgt gcgcggcttt ggcgaccgct tcggcattcc gctgccgaac gcgctgacgg tctggcagca gcgcaacgcg acgggcgcgc tggtgctgcc ctatcacctg gacggcagca agggcaacgc acagcgcgtc agcggcacgc cgcactcgtg ggacgacggc cagaacgcgt gggacggcgg gcgcatgtat cagtggccgc gctacaagaa cacggcgtcg atgggctact tccgggagag cgagctgccg ttccagttcg cgctcgcgaa cgcgttcacg atctgcgatg cgtaccactg ctcgatgcac gcgggcacca actccaaccg gatgttcatg tggaccggca ccaacggccc gaccggcgcg ggcgtggcct cggtggtgaa cgaatgggac gacatcggcc cctccacgtt cggctatgag tggaagacct atcccgagcg cttgcaggaa gccggcgtga gctggaaggt ctaccagaac atgccggaca acttcaccga caacgcgctt gccggcttca ggcagtaccg ccgcgcgaac gaggcgtcgg gcaagccggt gagcaacagc gacaaggtgg tttcgcccgc atacgatccg gcgagcgacg acgttcgcaa cccgctgtac aagggcatcg ccaacacgat gcccgacggc ggcttcctcg gcgccttcaa ggaagacatc cgtgcgggca agctgccgca ggtgtcgtgg gtggtggcgc cggccgcgta ttccgagcat ccggggccgt cgagcccggt gcagggcgcg tggtacatcc aggaggtgct cgatgcgctc accgcgacgc cggacgtctg gagcaagacc gtgctgctcg tgaatttcga cgagaacgac ggctacttcg accacgtgcc ttcgccgtcg gcgccgtcgc tgaatccgga cggctcgccg gccggcaaga ccacgctgcc cgaggccgac atcgcgttcg agcgcttcac ccatccgaag ccgccgggca ccaagagcca gccgcagccc gacgcccgcg tatatggccc cggcgtgcgc gtgccgatgt acgtgatctc gccgtggagc cgcggcggct gggtcaactc gcaggtgttc gatcacacgt cgacgctgcg tttcatcgag gcgcgcttcg gtgtgcgcga gcccaatatc tccgcgttcc gccgcgcggt gtgcggcgat ctgacgagcg cgttcaactt cgtgcgaccg aacaacgagc cgctgcccac gctggcgggg cgcaagaccc gagctcaggc cgatgcgctg cgcgccgcgc agcagcagat gccgcagatc gtgccgcctg cgaacggcct attgccgcgc caggagacag gcacgcggcc gtcgcgcgcg ttgccgtacg aactgcatgt caacgcggtc gcgcgcacgc tcgacggcac gctgcgcctc gtattcgcca acacggggca ggccgcggcc gtgttccacg tctacgacaa gctgaatctg ggccgcctgc cgcgccgata catggtcgag gccggcaagc agctcgacga cgtctgggcc gcgatgacgg acaacggcgg caagtacgac ctgtgggtgc tcgggcccaa cggcttgcac cgccatttca ccggcgatat gaaccgcctg cgcgcgggcg gcgcgcccgt gcccgaagtg cgggtggagt acgaccgcca gcacggcaac gtgcaattgc tgttgcgtaa cgaaggggcg cgcgagtgcg tgttcaccgt gcgcgcgaag gcgtaccgcg acgatggtcc gtggaccttc aaggtcaagg gcggcgccga gcgcaagcat cactggaagc tggacggcag cggcaattgg tacgacttcg tcgtgacctg cgacgccgac accggttact cgcgccgctt cgccggccgg gtggaaaccg gcgagcactc cgtcagcgat ccggcgatgg ggttcggcga tcggttctga

Details for BupsA.17253.b.A1.GE34756

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	85kDa
OBSERVED MW:	85kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	20

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRASPVHRS VNPYQGLRNM NARRRFLQGT ATTGIAAATL AAFPLSIRRA LAIPANNRTG TIRDVEHIVI LMQENRSFDN YFGTLRGVRG FGDRFGIPLP NALTVWQQRN ATGALVLPYH LDGSKGNAQR VSGTPHSWX

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgcg catcgccggt tcatcgctcg gtcaacccat accaaggact ccggaacatg aacgctcgtc gcagatttct gcaaggcacc gcaaccaccg gcatcgcggc cgccacgttg gccgcgtttc cgctcagcat tcgccgcgca ctcgcgattc ccgccaacaa ccgcaccgga accatccggg atgtcgagca catcgtcatc ctgatgcagg aaaaccgctc gttcgacaac tacttcggca cgctgcgcgg cgtgcgcggc tttggcgacc gcttcggcat tccgctgccg aacgcgctga cggtctggca gcagcgcaac gcgacgggcg cgctggtgct gccctatcac ctggacggca gcaagggcaa cgcacagcgc gtcagcggca cgccgcactc ntggganga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRASPVHRS VNPYQGLRNM NARRRFLQGT ATTGIAAATL AAFPLSIRRA LAIPANNRTG TIRDVEHIVI LMQENRSFDN YFGTLRGVRG FGDRFGIPLP NALTVWQQRN ATGALVLPYH LDGSKGNAQR VSGTPHSWDD GQNAWDGGRM YQWPRYKNTA SMGYFRESEL PFQFALANAF TICDAYHCSM HAGTNSNRMF MWTGTNGPTG AGVASVVNEW DDIGPSTFGY EWKTYPERLQ EAGVSWKVYQ NMPDNFTDNA LAGFRQYRRA NEASGKPVSN SDKVVSPAYD PASDDVRNPL YKGIANTMPD GGFLGAFKED IRAGKLPQVS WVVAPAAYSE HPGPSSPVQG AWYIQEVLDA LTATPDVWSK TVLLVNFDEN DGYFDHVPSP SAPSLNPDGS PAGKTTLPEA DIAFERFTHP KPPGTKSQPQ PDARVYGPGV RVPMYVISPW SRGGWVNSQV FDHTSTLRFI EARFGVREPN ISAFRRAVCG DLTSAFNFVR PNNEPLPTLA GRKTRAQADA LRAAQQQMPQ IVPPANGLLP RQETGTRPSR ALPYELHVNA VARTLDGTLR LVFANTGQAA AVFHVYDKLN LGRLPRRYMV EAGKQLDDVW AAMTDNGGKY DLWVLGPNGL HRHFTGDMNR LRAGGAPVPE VRVEYDRQHG NVQLLLRNEG ARECVFTVRA KAYRDDGPWT FKVKGGAERK HHWKLDGSGN WYDFVVTCDA DTGYSRRFAG RVETGEHSVS DPAMGFGDRF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcgcatcg ccggttcatc gctcggtcaa cccataccaa ggactccgga acatgaacgc tcgtcgcaga tttctgcaag gcaccgcaac caccggcatc gcggccgcca cgttggccgc gtttccgctc agcattcgcc gcgcactcgc gattcccgcc aacaaccgca ccggaaccat ccgggatgtc gagcacatcg tcatcctgat gcaggaaaac cgctcgttcg acaactactt cggcacgctg cgcggcgtgc gcggctttgg cgaccgcttc ggcattccgc tgccgaacgc gctgacggtc tggcagcagc gcaacgcgac gggcgcgctg gtgctgccct atcacctgga cggcagcaag ggcaacgcac agcgcgtcag cggcacgccg cactcgtggg acgacggcca gaacgcgtgg gacggcgggc gcatgtatca gtggccgcgc tacaagaaca cggcgtcgat gggctacttc cgggagagcg agctgccgtt ccagttcgcg ctcgcgaacg cgttcacgat ctgcgatgcg taccactgct cgatgcacgc gggcaccaac tccaaccgga tgttcatgtg gaccggcacc aacggcccga ccggcgcggg cgtggcctcg gtggtgaacg aatgggacga catcggcccc tccacgttcg gctatgagtg gaagacctat cccgagcgct tgcaggaagc cggcgtgagc tggaaggtct accagaacat gccggacaac ttcaccgaca acgcgcttgc cggcttcagg cagtaccgcc gcgcgaacga ggcgtcgggc aagccggtga gcaacagcga caaggtggtt tcgcccgcat acgatccggc gagcgacgac gttcgcaacc cgctgtacaa gggcatcgcc aacacgatgc ccgacggcgg cttcctcggc gccttcaagg aagacatccg tgcgggcaag ctgccgcagg tgtcgtgggt ggtggcgccg gccgcgtatt ccgagcatcc ggggccgtcg agcccggtgc agggcgcgtg gtacatccag gaggtgctcg atgcgctcac cgcgacgccg gacgtctgga gcaagaccgt gctgctcgtg aatttcgacg agaacgacgg ctacttcgac cacgtgcctt cgccgtcggc gccgtcgctg aatccggacg gctcgccggc cggcaagacc acgctgcccg aggccgacat cgcgttcgag cgcttcaccc atccgaagcc gccgggcacc aagagccagc cgcagcccga cgcccgcgta tatggccccg gcgtgcgcgt gccgatgtac gtgatctcgc cgtggagccg cggcggctgg gtcaactcgc aggtgttcga tcacacgtcg acgctgcgtt tcatcgaggc gcgcttcggt gtgcgcgagc ccaatatctc cgcgttccgc cgcgcggtgt gcggcgatct gacgagcgcg ttcaacttcg tgcgaccgaa caacgagccg ctgcccacgc tggcggggcg caagacccga gctcaggccg atgcgctgcg cgccgcgcag cagcagatgc cgcagatcgt gccgcctgcg aacggcctat tgccgcgcca ggagacaggc acgcggccgt cgcgcgcgtt gccgtacgaa ctgcatgtca acgcggtcgc gcgcacgctc gacggcacgc tgcgcctcgt attcgccaac acggggcagg ccgcggccgt gttccacgtc tacgacaagc tgaatctggg ccgcctgccg cgccgataca tggtcgaggc cggcaagcag ctcgacgacg tctgggccgc gatgacggac aacggcggca agtacgacct gtgggtgctc gggcccaacg gcttgcaccg ccatttcacc ggcgatatga accgcctgcg cgcgggcggc gcgcccgtgc ccgaagtgcg ggtggagtac gaccgccagc acggcaacgt gcaattgctg ttgcgtaacg aaggggcgcg cgagtgcgtg ttcaccgtgc gcgcgaaggc gtaccgcgac gatggtccgt ggaccttcaa ggtcaagggc ggcgccgagc gcaagcatca ctggaagctg gacggcagcg gcaattggta cgacttcgtc gtgacctgcg acgccgacac cggttactcg cgccgcttcg ccggccgggt ggaaaccggc gagcactccg tcagcgatcc ggcgatgggg ttcggcgatc ggttcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BupsA.17253.b.A2.GE34754

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	79kDa
OBSERVED MW:	80kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	42

Validated AA Sequence

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgattc ccgccaacaa ccgcaccgga accatccggg atgtcgagca catcgtcatc ctgatgcagg aaaaccgctc gttcgacaac tacttcggca cgctgcgcgg cgtgcgcggc tttggcgacc gcttcggcat tccgctgccg aacgcgctga cggtctggca gcagcgcaac gcgacgggcg cgctggtgct gccctatcac ctggacggca gcaagggcaa cgcacagcgc gtcagcggca cgccgcactc gtgggacgac ggccagaacg cgtgggacgg cgggcgcatg tatcagtggc cgcgctacaa gaacacggcg tcgatgggct acttccggga gagcgagctg ccgttccagt tcgcgctcgc gaacgcgttc acgatctgcg atgcgtacca ctgctcgatg cacgcgggca ccaactccaa ccggatgttc atgtggaccg gcaccaacgg cccgaccggc gcgggcgtgg cctcggtggt gaacgaatgg gacgacatcg gcccctccac gttcggctat gagtggaaga cctatcccga gcgcttgcag gaagccggcg tgagctggaa ggtctaccag aacatgccgg acaacttcac cgacaacgcg cttgccggct tcaggcagta ccgccgcgcg aacgaggcgt cgggcaagcc ggtgagcaac agcgacaagg tggtttcgcc cgcatacgat ccggcgagcg acgacgttcg caacccgctg tacnngggca tcgccaacac gatgcccgac ggcggcttcc tcggcgcctt caaggaagac atccgtgcgg gcaagctgcc gcnggngtcg tgggtggngg cgc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIPANNRTG TIRDVEHIVI LMQENRSFDN YFGTLRGVRG FGDRFGIPLP NALTVWQQRN ATGALVLPYH LDGSKGNAQR VSGTPHSWDD GQNAWDGGRM YQWPRYKNTA SMGYFRESEL PFQFALANAF TICDAYHCSM HAGTNSNRMF MWTGTNGPTG AGVASVVNEW DDIGPSTFGY EWKTYPERLQ EAGVSWKVYQ NMPDNFTDNA LAGFRQYRRA NEASGKPVSN SDKVVSPAYD PASDDVRNPL YKGIANTMPD GGFLGAFKED IRAGKLPQVS WVVAPAAYSE HPGPSSPVQG AWYIQEVLDA LTATPDVWSK TVLLVNFDEN DGYFDHVPSP SAPSLNPDGS PAGKTTLPEA DIAFERFTHP KPPGTKSQPQ PDARVYGPGV RVPMYVISPW SRGGWVNSQV FDHTSTLRFI EARFGVREPN ISAFRRAVCG DLTSAFNFVR PNNEPLPTLA GRKTRAQADA LRAAQQQMPQ IVPPANGLLP RQETGTRPSR ALPYELHVNA VARTLDGTLR LVFANTGQAA AVFHVYDKLN LGRLPRRYMV EAGKQLDDVW AAMTDNGGKY DLWVLGPNGL HRHFTGDMNR LRAGGAPVPE VRVEYDRQHG NVQLLLRNEG ARECVFTVRA KAYRDDGPWT FKVKGGAERK HHWKLDGSGN WYDFVVTCDA DTGYSRRFAG RVETGEHSVS DPAMGFGDRF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattcccgcc aacaaccgca ccggaaccat ccgggatgtc gagcacatcg tcatcctgat gcaggaaaac cgctcgttcg acaactactt cggcacgctg cgcggcgtgc gcggctttgg cgaccgcttc ggcattccgc tgccgaacgc gctgacggtc tggcagcagc gcaacgcgac gggcgcgctg gtgctgccct atcacctgga cggcagcaag ggcaacgcac agcgcgtcag cggcacgccg cactcgtggg acgacggcca gaacgcgtgg gacggcgggc gcatgtatca gtggccgcgc tacaagaaca cggcgtcgat gggctacttc cgggagagcg agctgccgtt ccagttcgcg ctcgcgaacg cgttcacgat ctgcgatgcg taccactgct cgatgcacgc gggcaccaac tccaaccgga tgttcatgtg gaccggcacc aacggcccga ccggcgcggg cgtggcctcg gtggtgaacg aatgggacga catcggcccc tccacgttcg gctatgagtg gaagacctat cccgagcgct tgcaggaagc cggcgtgagc tggaaggtct accagaacat gccggacaac ttcaccgaca acgcgcttgc cggcttcagg cagtaccgcc gcgcgaacga ggcgtcgggc aagccggtga gcaacagcga caaggtggtt tcgcccgcat acgatccggc gagcgacgac gttcgcaacc cgctgtacaa gggcatcgcc aacacgatgc ccgacggcgg cttcctcggc gccttcaagg aagacatccg tgcgggcaag ctgccgcagg tgtcgtgggt ggtggcgccg gccgcgtatt ccgagcatcc ggggccgtcg agcccggtgc agggcgcgtg gtacatccag gaggtgctcg atgcgctcac cgcgacgccg gacgtctgga gcaagaccgt gctgctcgtg aatttcgacg agaacgacgg ctacttcgac cacgtgcctt cgccgtcggc gccgtcgctg aatccggacg gctcgccggc cggcaagacc acgctgcccg aggccgacat cgcgttcgag cgcttcaccc atccgaagcc gccgggcacc aagagccagc cgcagcccga cgcccgcgta tatggccccg gcgtgcgcgt gccgatgtac gtgatctcgc cgtggagccg cggcggctgg gtcaactcgc aggtgttcga tcacacgtcg acgctgcgtt tcatcgaggc gcgcttcggt gtgcgcgagc ccaatatctc cgcgttccgc cgcgcggtgt gcggcgatct gacgagcgcg ttcaacttcg tgcgaccgaa caacgagccg ctgcccacgc tggcggggcg caagacccga gctcaggccg atgcgctgcg cgccgcgcag cagcagatgc cgcagatcgt gccgcctgcg aacggcctat tgccgcgcca ggagacaggc acgcggccgt cgcgcgcgtt gccgtacgaa ctgcatgtca acgcggtcgc gcgcacgctc gacggcacgc tgcgcctcgt attcgccaac acggggcagg ccgcggccgt gttccacgtc tacgacaagc tgaatctggg ccgcctgccg cgccgataca tggtcgaggc cggcaagcag ctcgacgacg tctgggccgc gatgacggac aacggcggca agtacgacct gtgggtgctc gggcccaacg gcttgcaccg ccatttcacc ggcgatatga accgcctgcg cgcgggcggc gcgcccgtgc ccgaagtgcg ggtggagtac gaccgccagc acggcaacgt gcaattgctg ttgcgtaacg aaggggcgcg cgagtgcgtg ttcaccgtgc gcgcgaaggc gtaccgcgac gatggtccgt ggaccttcaa ggtcaagggc ggcgccgagc gcaagcatca ctggaagctg gacggcagcg gcaattggta cgacttcgtc gtgacctgcg acgccgacac cggttactcg cgccgcttcg ccggccgggt ggaaaccggc gagcactccg tcagcgatcc ggcgatgggg ttcggcgatc ggttcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BupsA.17253.b.A1.PS01461

PURIFICATION DATe:	4/12/2012
CONCENTRATION:	14.6mg/ml
OBSERVED MW:	83kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	20

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRASPVHRS VNPYQGLRNM NARRRFLQGT ATTGIAAATL AAFPLSIRRA LAIPANNRTG TIRDVEHIVI LMQENRSFDN YFGTLRGVRG FGDRFGIPLP NALTVWQQRN ATGALVLPYH LDGSKGNAQR VSGTPHSWX

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCGCATCG CCGGTTCATC GCTCGGTCAA CCCATACCAA GGACTCCGGA ACATGAACGC TCGTCGCAGA TTTCTGCAAG GCACCGCAAC CACCGGCATC GCGGCCGCCA CGTTGGCCGC GTTTCCGCTC AGCATTCGCC GCGCACTCGC GATTCCCGCC AACAACCGCA CCGGAACCAT CCGGGATGTC GAGCACATCG TCATCCTGAT GCAGGAAAAC CGCTCGTTCG ACAACTACTT CGGCACGCTG CGCGGCGTGC GCGGCTTTGG CGACCGCTTC GGCATTCCGC TGCCGAACGC GCTGACGGTC TGGCAGCAGC GCAACGCGAC GGGCGCGCTG GTGCTGCCCT ATCACCTGGA CGGCAGCAAG GGCAACGCAC AGCGCGTCAG CGGCACGCCG CACTCGTGGG ACGACGGCCA GAACGCGTGG GACGGCGGGC GCATGTATCA GTGGCCGCGC TACAAGAACA CGGCGTCGAT GGGCTACTTC CGGGAGAGCG AGCTGCCGTT CCAGTTCGCG CTCGCGAACG CGTTCACGAT CTGCGATGCG TACCACTGCT CGATGCACGC GGGCACCAAC TCCAACCGGA TGTTCATGTG GACCGGCACC AACGGCCCGA CCGGCGCGGG CGTGGCCTCG GTGGTGAACG AATGGGACGA CATCGGCCCC TCCACGTTCG GCTATGAGTG GAAGACCTAT CCCGAGCGCT TGCAGGAAGC CGGCGTGAGC TGGAAGGTCT ACCAGAACAT GCCGGACAAC TTCACCGACA ACGCGCTTGC CGGCTTCAGG CAGTACCGCC GCGCGAACGA GGCGTCGGGC AAGCCGGTGA GCAACAGCGA CAAGGTGGTT TCGCCCGCAT ACGATCCGGC GAGCGACGAC GTTCGCAACC CGCTGTACAA GGGCATCGCC AACACGATGC CCGACGGCGG CTTCCTCGGC GCCTTCAAGG AAGACATCCG TGCGGGCAAG CTGCCGCAGG TGTCGTGGGT GGTGGCGCCG GCCGCGTATT CCGAGCATCC GGGGCCGTCG AGCCCGGTGC AGGGCGCGTG GTACATCCAG GAGGTGCTCG ATGCGCTCAC CGCGACGCCG GACGTCTGGA GCAAGACCGT GCTGCTCGTG AATTTCGACG AGAACGACGG CTACTTCGAC CACGTGCCTT CGCCGTCGGC GCCGTCGCTG AATCCGGACG GCTCGCCGGC CGGCAAGACC ACGCTGCCCG AGGCCGACAT CGCGTTCGAG CGCTTCACCC ATCCGAAGCC GCCGGGCACC AAGAGCCAGC CGCAGCCCGA CGCCCGCGTA TATGGCCCCG GCGTGCGCGT GCCGATGTAC GTGATCTCGC CGTGGAGCCG CGGCGGCTGG GTCAACTCGC AGGTGTTCGA TCACACGTCG ACGCTGCGTT TCATCGAGGC GCGCTTCGGT GTGCGCGAGC CCAATATCTC CGCGTTCCGC CGCGCGGTGT GCGGCGATCT GACGAGCGCG TTCAACTTCG TGCGACCGAA CAACGAGCCG CTGCCCACGC TGGCGGGGCG CAAGACCCGA GCTCAGGCCG ATGCGCTGCG CGCCGCGCAG CAGCAGATGC CGCAGATCGT GCCGCCTGCG AACGGCCTAT TGCCGCGCCA GGAGACAGGC ACGCGGCCGT CGCGCGCGTT GCCGTACGAA CTGCATGTCA ACGCGGTCGC GCGCACGCTC GACGGCACGC TGCGCCTCGT ATTCGCCAAC ACGGGGCAGG CCGCGGCCGT GTTCCACGTC TACGACAAGC TGAATCTGGG CCGCCTGCCG CGCCGATACA TGGTCGAGGC CGGCAAGCAG CTCGACGACG TCTGGGCCGC GATGACGGAC AACGGCGGCA AGTACGACCT GTGGGTGCTC GGGCCCAACG GCTTGCACCG CCATTTCACC GGCGATATGA ACCGCCTGCG CGCGGGCGGC GCGCCCGTGC CCGAAGTGCG GGTGGAGTAC GACCGCCAGC ACGGCAACGT GCAATTGCTG TTGCGTAACG AAGGGGCGCG CGAGTGCGTG TTCACCGTGC GCGCGAAGGC GTACCGCGAC GATGGTCCGT GGACCTTCAA GGTCAAGGGC GGCGCCGAGC GCAAGCATCA CTGGAAGCTG GACGGCAGCG GCAATTGGTA CGACTTCGTC GTGACCTGCG ACGCCGACAC CGGTTACTCG CGCCGCTTCG CCGGCCGGGT GGAAACCGGC GAGCACTCCG TCAGCGATCC GGCGATGGGG TTCGGCGATC GGTTCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA

Details for BupsA.17253.b.A1.PS01462

PURIFICATION DATe:	4/12/2012
CONCENTRATION:	12.9mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	20

Protocol Notes

notes unavailable

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRASPVHRS VNPYQGLRNM NARRRFLQGT ATTGIAAATL AAFPLSIRRA LAIPANNRTG TIRDVEHIVI LMQENRSFDN YFGTLRGVRG FGDRFGIPLP NALTVWQQRN ATGALVLPYH LDGSKGNAQR VSGTPHSWX

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)