BupsA.17253.a

Non-hemolytic phospholipase C (3.1.4.3)

CENTER ID:	BupsA.17253.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17253.a.A1.GE34755	full length		1	705
BupsA.17253.a.A2.GE34753	mature protein		36	705

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17253.a.A1.PS01478	full length		1	705

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_131170
PATRIC ID:	fig\|320372.6.peg.551
RefSeq:	YP_331963.1
UniProt:	Q3JWU0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSESRRRFL HTVAQSAGAA AALTVFPESI RRALAIPAAS RTGTLRDVEH IVVFMQENRS FDHYFGHLRG VRGYNDRFPI PLPNGKPVWY QPSKANPGKP VLPFRLNTLT TSAQCIGDLD HSWYKTHAAI DGGRYDQWPA NKTDMTMGYH VREDIPFHYA LADAFTVCDN YFCSLPGPTH PNRSYLMTGT VDPTGRYGGP LLDNSDYVDG DVPPKYDLLT WTTYPERLEA NGISWQIYQQ GTTGADPLNG NYGTNILQNF ANFINAKPGS SLYRRAQTAR TLDDLKADVL ANKLPQVSWL LPPAAFSEHP RYTPAYGANY TSQILDALTS NPDVWRKTVL FIMYDENDGF FDHVVPPQPA TTRAQGLSTV TVDGEIHDVV NPGRGGSYTA DGLPYGLGPR VPMTVVSPWT KGGFVCSQVF DHTSVIRFIA ARFGIDEPNI TPWRRAVCGD LTSAFDFRTP DATLPPLPDT SGYRSIADQL CSTKPAPAVP AAPSPVDPQE PGVRPARALP YELHVNAEVH GGARLRIEFA NRGEQGAHFY VYASNRSDGP WRYTVGAHRS LHETFDLAAT NGAYAFAVYG PNGFVRVFEG LAAAGGHGRQ AAARPEVKAG YDVANGNLYL KLANHGAQSV QLTLADNAYG APARRITLRG GDEQIESWAL ASSHQWYDVT VSDGATGQFV RRFAGHVENG KPSYSDPAAI APVTG

NT Sequence

atgacatcag aaagccgccg ccgtttcctg cataccgtcg cgcaatccgc gggcgccgcc gccgcgctca ccgtgtttcc cgaatcgatt cgccgcgcac tcgcgattcc cgccgcgtcg cgcacgggca cgctgcgcga tgtcgagcac atcgtcgtgt tcatgcagga aaaccgctcg ttcgaccatt acttcggaca cctgcgcggt gtgcgcggct acaacgaccg ctttccgatt ccgctgccga acggcaaacc ggtctggtat cagccgtcga aggcgaatcc gggcaagccg gtgctgccgt ttcgcctgaa cacgctgacg acgagcgcgc agtgcatcgg cgatctcgat cactcgtggt acaagacgca tgcggcgatc gacggcggcc gctacgatca gtggcccgcg aacaagaccg acatgacgat gggctaccac gtgcgcgagg acatcccgtt ccactacgcg ctcgccgacg cgttcaccgt ctgcgacaac tacttctgct cgctgccggg cccgacgcat ccgaaccgct cgtatctgat gacgggcacg gtcgatccga ccggccggta cggcggcccg ctgctcgaca acagcgatta cgtggacggc gacgtgccgc cgaaatacga tctgctcacc tggacgacgt accccgaacg cctcgaggcg aacggcattt cgtggcagat ctatcagcag ggcacgacgg gggccgatcc gctgaacggc aactacggga cgaacatcct gcagaacttc gcgaacttca tcaacgcgaa gccgggctcg tcgctgtatc ggcgcgcgca gaccgcgcgc acgctcgacg atctgaaggc cgacgtgctc gcgaacaagc tgccgcaggt gtcgtggctg ctgccgcccg cggcgttctc cgagcatccg agatacacgc ccgcgtacgg cgcgaactac acgtcgcaga tcctcgacgc gctgacgtcg aaccccgacg tgtggcgcaa gaccgtgctc ttcatcatgt acgacgagaa cgacggcttc ttcgatcacg tcgtgccgcc gcagccggcg acgacgcgcg cgcagggcct gtcgaccgtg accgtcgacg gcgagatcca cgacgtcgtg aacccgggcc gcggcggcag ctacacggcc gacggcctgc cgtacggcct cggcccgcgc gtgccgatga cggtcgtgtc gccgtggacg aagggcggct tcgtgtgctc gcaggtgttc gaccatacgt cggtgatccg cttcatcgcc gcgcgcttcg gcatcgacga gccgaacatc acgccgtggc gccgcgcggt gtgcggcgat ctcacgtcgg cgttcgactt ccgcacgccg gacgcgacgc tgccgccgtt gcccgacacg agcggctatc ggtcgatcgc cgatcagctc tgctcgacga agcccgcgcc cgccgtgccc gccgcgccga gccccgtcga tccgcaggag cccggcgtgc gcccggcgcg cgcgctgcct tacgagctgc acgtgaacgc cgaggtgcac ggcggcgcgc gcctgcgcat cgagttcgcg aatcgcggcg agcaaggcgc gcacttctat gtgtacgcat cgaatcgcag cgacggcccg tggcgctaca cggtcggcgc gcaccgctcg ttgcacgaaa cgttcgatct cgccgcgacg aacggcgcgt acgcgttcgc cgtgtatggt ccgaacggct tcgtgcgcgt gttcgaaggc ctcgccgcgg ccggaggcca cggccggcaa gcggccgcgc gccccgaggt gaaggcgggc tacgacgtcg cgaacggcaa cctgtatctg aagctcgcga atcatggcgc acagagcgtg caactgacgc tcgccgacaa cgcgtacggc gcgccggcgc gccggatcac gctgcgcggc ggcgatgagc agatcgaatc gtgggcgctg gcgtcgagcc atcaatggta tgacgtgacg gtgtcggacg gcgcgaccgg ccagttcgtg cgccgcttcg cggggcacgt cgagaacggc aagccgagct attccgatcc ggcggcgatc gcgcccgtca cgggctga

Details for BupsA.17253.a.A1.GE34755

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	79kDa
OBSERVED MW:	79kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	35

Validated AA Sequence

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacat cagaaagccg ccgccgtttc ctgcataccg tcgcgcaatc cgcgggcgcc gccgccgcgc tcaccgtgtt tcccgaatcg attcgccgcg cactcgcgat tcccgccgcg tcgcgcacgg gcacgctgcg cgatgtcgag cacatcgtcg tgttcatgca ggaaaaccgc tcgttcgacc attacttcgg acacctgcgc ggtgtgcgcg gctacaacga ccgctttccg attccgctgc cgaacggcaa accggtctgg tatcagccgt cgaaggcgaa tccgggcaag ccggtgctgc cgtttcgcct gaacacgctg acgacgagcg cgcagtgcat cggcgatctc gatcactcgt ggtacaagac gcatgcggcg atcgacggcg gncgctacga tcagtggncc gcgaacaaga ccgacatgac gatgggctac cacgtgcgcg aggacatccc gttccactac gcgctcgccg acgcgttcac cgtctgcgac nactacttct gctcgctgcc gggcccgacg catccgaacc gctcgtatct gatgacgggn acggtcgatc cgaccggccg gtacggcggn ccgctgctcg acaacngcga ttacgtggac ggngacgtgc cnccnaaata cgatctgctc acctgnacga nntaccccga angcctcgag gngaacggna tttcgtg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTSESRRRF LHTVAQSAGA AAALTVFPES IRRALAIPAA SRTGTLRDVE HIVVFMQENR SFDHYFGHLR GVRGYNDRFP IPLPNGKPVW YQPSKANPGK PVLPFRLNTL TTSAQCIGDL DHSWYKTHAA IDGGRYDQWP ANKTDMTMGY HVREDIPFHY ALADAFTVCD NYFCSLPGPT HPNRSYLMTG TVDPTGRYGG PLLDNSDYVD GDVPPKYDLL TWTTYPERLE ANGISWQIYQ QGTTGADPLN GNYGTNILQN FANFINAKPG SSLYRRAQTA RTLDDLKADV LANKLPQVSW LLPPAAFSEH PRYTPAYGAN YTSQILDALT SNPDVWRKTV LFIMYDENDG FFDHVVPPQP ATTRAQGLST VTVDGEIHDV VNPGRGGSYT ADGLPYGLGP RVPMTVVSPW TKGGFVCSQV FDHTSVIRFI AARFGIDEPN ITPWRRAVCG DLTSAFDFRT PDATLPPLPD TSGYRSIADQ LCSTKPAPAV PAAPSPVDPQ EPGVRPARAL PYELHVNAEV HGGARLRIEF ANRGEQGAHF YVYASNRSDG PWRYTVGAHR SLHETFDLAA TNGAYAFAVY GPNGFVRVFE GLAAAGGHGR QAAARPEVKA GYDVANGNLY LKLANHGAQS VQLTLADNAY GAPARRITLR GGDEQIESWA LASSHQWYDV TVSDGATGQF VRRFAGHVEN GKPSYSDPAA IAPVTG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacatcagaa agccgccgcc gtttcctgca taccgtcgcg caatccgcgg gcgccgccgc cgcgctcacc gtgtttcccg aatcgattcg ccgcgcactc gcgattcccg ccgcgtcgcg cacgggcacg ctgcgcgatg tcgagcacat cgtcgtgttc atgcaggaaa accgctcgtt cgaccattac ttcggacacc tgcgcggtgt gcgcggctac aacgaccgct ttccgattcc gctgccgaac ggcaaaccgg tctggtatca gccgtcgaag gcgaatccgg gcaagccggt gctgccgttt cgcctgaaca cgctgacgac gagcgcgcag tgcatcggcg atctcgatca ctcgtggtac aagacgcatg cggcgatcga cggcggccgc tacgatcagt ggcccgcgaa caagaccgac atgacgatgg gctaccacgt gcgcgaggac atcccgttcc actacgcgct cgccgacgcg ttcaccgtct gcgacaacta cttctgctcg ctgccgggcc cgacgcatcc gaaccgctcg tatctgatga cgggcacggt cgatccgacc ggccggtacg gcggcccgct gctcgacaac agcgattacg tggacggcga cgtgccgccg aaatacgatc tgctcacctg gacgacgtac cccgaacgcc tcgaggcgaa cggcatttcg tggcagatct atcagcaggg cacgacgggg gccgatccgc tgaacggcaa ctacgggacg aacatcctgc agaacttcgc gaacttcatc aacgcgaagc cgggctcgtc gctgtatcgg cgcgcgcaga ccgcgcgcac gctcgacgat ctgaaggccg acgtgctcgc gaacaagctg ccgcaggtgt cgtggctgct gccgcccgcg gcgttctccg agcatccgag atacacgccc gcgtacggcg cgaactacac gtcgcagatc ctcgacgcgc tgacgtcgaa ccccgacgtg tggcgcaaga ccgtgctctt catcatgtac gacgagaacg acggcttctt cgatcacgtc gtgccgccgc agccggcgac gacgcgcgcg cagggcctgt cgaccgtgac cgtcgacggc gagatccacg acgtcgtgaa cccgggccgc ggcggcagct acacggccga cggcctgccg tacggcctcg gcccgcgcgt gccgatgacg gtcgtgtcgc cgtggacgaa gggcggcttc gtgtgctcgc aggtgttcga ccatacgtcg gtgatccgct tcatcgccgc gcgcttcggc atcgacgagc cgaacatcac gccgtggcgc cgcgcggtgt gcggcgatct cacgtcggcg ttcgacttcc gcacgccgga cgcgacgctg ccgccgttgc ccgacacgag cggctatcgg tcgatcgccg atcagctctg ctcgacgaag cccgcgcccg ccgtgcccgc cgcgccgagc cccgtcgatc cgcaggagcc cggcgtgcgc ccggcgcgcg cgctgcctta cgagctgcac gtgaacgccg aggtgcacgg cggcgcgcgc ctgcgcatcg agttcgcgaa tcgcggcgag caaggcgcgc acttctatgt gtacgcatcg aatcgcagcg acggcccgtg gcgctacacg gtcggcgcgc accgctcgtt gcacgaaacg ttcgatctcg ccgcgacgaa cggcgcgtac gcgttcgccg tgtatggtcc gaacggcttc gtgcgcgtgt tcgaaggcct cgccgcggcc ggaggccacg gccggcaagc ggccgcgcgc cccgaggtga aggcgggcta cgacgtcgcg aacggcaacc tgtatctgaa gctcgcgaat catggcgcac agagcgtgca actgacgctc gccgacaacg cgtacggcgc gccggcgcgc cggatcacgc tgcgcggcgg cgatgagcag atcgaatcgt gggcgctggc gtcgagccat caatggtatg acgtgacggt gtcggacggc gcgaccggcc agttcgtgcg ccgcttcgcg gggcacgtcg agaacggcaa gccgagctat tccgatccgg cggcgatcgc gcccgtcacg ggcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BupsA.17253.a.A2.GE34753

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	75kDa
OBSERVED MW:	75kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	22

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMIPAASRTG TLRDVEHIVV FMQENRSFDH YFGHLRGVRG YNDRFPIPLP NGKPVWYQPS KANPGKPVLP FRLNTLTTSA QCIGDLDHSW YKTHAAIDGG RYDQWPANKT DMTMGYHVRE DIPFHYALAX AFT

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgattc ccgccgcgtc gcgcacgggc acgctgcgcg atgtcgagca catcgtcgtg ttcatgcagg aaaaccgctc gttcgaccat tacttcggac acctgcgcgg tgtgcgcggc tacaacgacc gctttccgat tccgctgccg aacggcaaac cggtctggta tcagccgtcg aaggcgaatc cgggcaagcc ggtgctgccg tttcgcctga acacgctgac gacgagcgcg cagtgcatcg gcgatctcga tcactcgtgg tacaagacgc atgcggcgat cgacggcggc cgctacgatc agtggcccgc gaacaagacc gacatgacga tgggctacca cgtgcgcgag gacatcccgt tccactacgc gctcgccgan gcgttcaccg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIPAASRTG TLRDVEHIVV FMQENRSFDH YFGHLRGVRG YNDRFPIPLP NGKPVWYQPS KANPGKPVLP FRLNTLTTSA QCIGDLDHSW YKTHAAIDGG RYDQWPANKT DMTMGYHVRE DIPFHYALAD AFTVCDNYFC SLPGPTHPNR SYLMTGTVDP TGRYGGPLLD NSDYVDGDVP PKYDLLTWTT YPERLEANGI SWQIYQQGTT GADPLNGNYG TNILQNFANF INAKPGSSLY RRAQTARTLD DLKADVLANK LPQVSWLLPP AAFSEHPRYT PAYGANYTSQ ILDALTSNPD VWRKTVLFIM YDENDGFFDH VVPPQPATTR AQGLSTVTVD GEIHDVVNPG RGGSYTADGL PYGLGPRVPM TVVSPWTKGG FVCSQVFDHT SVIRFIAARF GIDEPNITPW RRAVCGDLTS AFDFRTPDAT LPPLPDTSGY RSIADQLCST KPAPAVPAAP SPVDPQEPGV RPARALPYEL HVNAEVHGGA RLRIEFANRG EQGAHFYVYA SNRSDGPWRY TVGAHRSLHE TFDLAATNGA YAFAVYGPNG FVRVFEGLAA AGGHGRQAAA RPEVKAGYDV ANGNLYLKLA NHGAQSVQLT LADNAYGAPA RRITLRGGDE QIESWALASS HQWYDVTVSD GATGQFVRRF AGHVENGKPS YSDPAAIAPV TG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gattcccgcc gcgtcgcgca cgggcacgct gcgcgatgtc gagcacatcg tcgtgttcat gcaggaaaac cgctcgttcg accattactt cggacacctg cgcggtgtgc gcggctacaa cgaccgcttt ccgattccgc tgccgaacgg caaaccggtc tggtatcagc cgtcgaaggc gaatccgggc aagccggtgc tgccgtttcg cctgaacacg ctgacgacga gcgcgcagtg catcggcgat ctcgatcact cgtggtacaa gacgcatgcg gcgatcgacg gcggccgcta cgatcagtgg cccgcgaaca agaccgacat gacgatgggc taccacgtgc gcgaggacat cccgttccac tacgcgctcg ccgacgcgtt caccgtctgc gacaactact tctgctcgct gccgggcccg acgcatccga accgctcgta tctgatgacg ggcacggtcg atccgaccgg ccggtacggc ggcccgctgc tcgacaacag cgattacgtg gacggcgacg tgccgccgaa atacgatctg ctcacctgga cgacgtaccc cgaacgcctc gaggcgaacg gcatttcgtg gcagatctat cagcagggca cgacgggggc cgatccgctg aacggcaact acgggacgaa catcctgcag aacttcgcga acttcatcaa cgcgaagccg ggctcgtcgc tgtatcggcg cgcgcagacc gcgcgcacgc tcgacgatct gaaggccgac gtgctcgcga acaagctgcc gcaggtgtcg tggctgctgc cgcccgcggc gttctccgag catccgagat acacgcccgc gtacggcgcg aactacacgt cgcagatcct cgacgcgctg acgtcgaacc ccgacgtgtg gcgcaagacc gtgctcttca tcatgtacga cgagaacgac ggcttcttcg atcacgtcgt gccgccgcag ccggcgacga cgcgcgcgca gggcctgtcg accgtgaccg tcgacggcga gatccacgac gtcgtgaacc cgggccgcgg cggcagctac acggccgacg gcctgccgta cggcctcggc ccgcgcgtgc cgatgacggt cgtgtcgccg tggacgaagg gcggcttcgt gtgctcgcag gtgttcgacc atacgtcggt gatccgcttc atcgccgcgc gcttcggcat cgacgagccg aacatcacgc cgtggcgccg cgcggtgtgc ggcgatctca cgtcggcgtt cgacttccgc acgccggacg cgacgctgcc gccgttgccc gacacgagcg gctatcggtc gatcgccgat cagctctgct cgacgaagcc cgcgcccgcc gtgcccgccg cgccgagccc cgtcgatccg caggagcccg gcgtgcgccc ggcgcgcgcg ctgccttacg agctgcacgt gaacgccgag gtgcacggcg gcgcgcgcct gcgcatcgag ttcgcgaatc gcggcgagca aggcgcgcac ttctatgtgt acgcatcgaa tcgcagcgac ggcccgtggc gctacacggt cggcgcgcac cgctcgttgc acgaaacgtt cgatctcgcc gcgacgaacg gcgcgtacgc gttcgccgtg tatggtccga acggcttcgt gcgcgtgttc gaaggcctcg ccgcggccgg aggccacggc cggcaagcgg ccgcgcgccc cgaggtgaag gcgggctacg acgtcgcgaa cggcaacctg tatctgaagc tcgcgaatca tggcgcacag agcgtgcaac tgacgctcgc cgacaacgcg tacggcgcgc cggcgcgccg gatcacgctg cgcggcggcg atgagcagat cgaatcgtgg gcgctggcgt cgagccatca atggtatgac gtgacggtgt cggacggcgc gaccggccag ttcgtgcgcc gcttcgcggg gcacgtcgag aacggcaagc cgagctattc cgatccggcg gcgatcgcgc ccgtcacggg caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for BupsA.17253.a.A1.PS01478

PURIFICATION DATe:	4/27/2012
CONCENTRATION:	42.77mg/ml
OBSERVED MW:	76kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	11
VIAL VOLUME:	110µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	35

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACATCAGAA AGCCGCCGCC GTTTCCTGCA TACCGTCGCG CAATCCGCGG GCGCCGCCGC CGCGCTCACC GTGTTTCCCG AATCGATTCG CCGCGCACTC GCGATTCCCG CCGCGTCGCG CACGGGCACG CTGCGCGATG TCGAGCACAT CGTCGTGTTC ATGCAGGAAA ACCGCTCGTT CGACCATTAC TTCGGACACC TGCGCGGTGT GCGCGGCTAC AACGACCGCT TTCCGATTCC GCTGCCGAAC GGCAAACCGG TCTGGTATCA GCCGTCGAAG GCGAATCCGG GCAAGCCGGT GCTGCCGTTT CGCCTGAACA CGCTGACGAC GAGCGCGCAG TGCATCGGCG ATCTCGATCA CTCGTGGTAC AAGACGCATG CGGCGATCGA CGGCGGCCGC TACGATCAGT GGCCCGCGAA CAAGACCGAC ATGACGATGG GCTACCACGT GCGCGAGGAC ATCCCGTTCC ACTACGCGCT CGCCGACGCG TTCACCGTCT GCGACAACTA CTTCTGCTCG CTGCCGGGCC CGACGCATCC GAACCGCTCG TATCTGATGA CGGGCACGGT CGATCCGACC GGCCGGTACG GCGGCCCGCT GCTCGACAAC AGCGATTACG TGGACGGCGA CGTGCCGCCG AAATACGATC TGCTCACCTG GACGACGTAC CCCGAACGCC TCGAGGCGAA CGGCATTTCG TGGCAGATCT ATCAGCAGGG CACGACGGGG GCCGATCCGC TGAACGGCAA CTACGGGACG AACATCCTGC AGAACTTCGC GAACTTCATC AACGCGAAGC CGGGCTCGTC GCTGTATCGG CGCGCGCAGA CCGCGCGCAC GCTCGACGAT CTGAAGGCCG ACGTGCTCGC GAACAAGCTG CCGCAGGTGT CGTGGCTGCT GCCGCCCGCG GCGTTCTCCG AGCATCCGAG ATACACGCCC GCGTACGGCG CGAACTACAC GTCGCAGATC CTCGACGCGC TGACGTCGAA CCCCGACGTG TGGCGCAAGA CCGTGCTCTT CATCATGTAC GACGAGAACG ACGGCTTCTT CGATCACGTC GTGCCGCCGC AGCCGGCGAC GACGCGCGCG CAGGGCCTGT CGACCGTGAC CGTCGACGGC GAGATCCACG ACGTCGTGAA CCCGGGCCGC GGCGGCAGCT ACACGGCCGA CGGCCTGCCG TACGGCCTCG GCCCGCGCGT GCCGATGACG GTCGTGTCGC CGTGGACGAA GGGCGGCTTC GTGTGCTCGC AGGTGTTCGA CCATACGTCG GTGATCCGCT TCATCGCCGC GCGCTTCGGC ATCGACGAGC CGAACATCAC GCCGTGGCGC CGCGCGGTGT GCGGCGATCT CACGTCGGCG TTCGACTTCC GCACGCCGGA CGCGACGCTG CCGCCGTTGC CCGACACGAG CGGCTATCGG TCGATCGCCG ATCAGCTCTG CTCGACGAAG CCCGCGCCCG CCGTGCCCGC CGCGCCGAGC CCCGTCGATC CGCAGGAGCC CGGCGTGCGC CCGGCGCGCG CGCTGCCTTA CGAGCTGCAC GTGAACGCCG AGGTGCACGG CGGCGCGCGC CTGCGCATCG AGTTCGCGAA TCGCGGCGAG CAAGGCGCGC ACTTCTATGT GTACGCATCG AATCGCAGCG ACGGCCCGTG GCGCTACACG GTCGGCGCGC ACCGCTCGTT GCACGAAACG TTCGATCTCG CCGCGACGAA CGGCGCGTAC GCGTTCGCCG TGTATGGTCC GAACGGCTTC GTGCGCGTGT TCGAAGGCCT CGCCGCGGCC GGAGGCCACG GCCGGCAAGC GGCCGCGCGC CCCGAGGTGA AGGCGGGCTA CGACGTCGCG AACGGCAACC TGTATCTGAA GCTCGCGAAT CATGGCGCAC AGAGCGTGCA ACTGACGCTC GCCGACAACG CGTACGGCGC GCCGGCGCGC CGGATCACGC TGCGCGGCGG CGATGAGCAG ATCGAATCGT GGGCGCTGGC GTCGAGCCAT CAATGGTATG ACGTGACGGT GTCGGACGGC GCGACCGGCC AGTTCGTGCG CCGCTTCGCG GGGCACGTCG AGAACGGCAA GCCGAGCTAT TCCGATCCGG CGGCGATCGC GCCCGTCACG GGCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA