SSGCID: Target Page

BupsA.17229.a

4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1 (IspH/LytB) (IspG/H)

CENTER ID:	BupsA.17229.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17229.a.B1.GE41156	full length		1	326

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|272560.6.peg.1001
RefSeq:	YP_107546.1
UniProt:	Q63WH0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSTDTLSGQ VAAADAEILL AQPRGFCAGV DRAIEIVERA IAMHGAPIYV RHEIVHNKYV VEDLKKKGAI FVEELEEVPS GNTVIFSAHG VSKAVRDEAA VRGLRIYDAT CPLVTKVHVE VAKMRQEGVD IVMIGHKGHP EVEGTMGQVE RGMHLVESVE DVRRLELPDP ERVALVTQTT LSVDDAAEII GALKAKFPAI REPKKQDICY ATQNRQDAVK FMAPQCDVVI VVGSPNSSNS SRLREVAEKR GVAAYMVDAP EQIDPAWVAG KRRIGVTAGA SAPEVLAQAV IARLRELGVT NVRALEGIEE NVSFPLPRGL NLSSAA

NT Sequence

atgagctcca ccgatacgtt gtccggccag gttgccgcgg ccgacgccga gatcctgctc gcccagccgc gcggcttctg cgcgggtgtc gaccgcgcga tcgagatcgt cgagcgtgcg attgcgatgc acggcgcgcc gatctacgtg cgccacgaga tcgtgcacaa caagtacgtc gtcgaggatc tgaagaagaa gggcgcgatt tttgtcgagg aactcgagga agtgccgtcc ggcaacacgg tgatcttcag cgcgcacggc gtgtcgaagg cggtgcgcga cgaggcggcc gtgcgtgggc tgcgcatcta cgatgcgacg tgcccgctcg tcacgaaagt gcacgtcgag gtcgcgaaga tgcgccagga aggcgtcgac atcgtgatga tcggccacaa ggggcatccg gaggtcgagg gcacgatggg gcaggtcgag cgcggcatgc acctcgtcga gagcgtcgag gacgtgcgcc ggctcgagct gcccgatccc gagcgcgtcg cgctcgtcac gcagacgacg ctgtccgtcg acgacgcggc cgagatcatc ggcgcgttga aggcgaagtt tcccgcgatc cgcgagccga agaagcagga catctgctac gcgacgcaga accgccagga cgcggtgaag ttcatggcgc cgcagtgcga cgtcgtgatc gtcgtcggca gcccgaacag ctcgaattcg agccgcctgc gcgaggtcgc cgagaagcgc ggcgtcgccg cctacatggt cgacgcgccc gagcagatcg atccggcctg ggtcgccggc aagcgccgga tcggcgtgac cgccggcgcg tccgcgcccg aggtgctcgc gcaggccgtg atcgcgcgct tgcgcgagct cggcgtgacc aacgtgcggg cgctcgaggg catcgaggag aacgtgtcgt tcccattgcc gcgtggcctg aatctgtctt ccgccgcctg a

Details for BupsA.17229.a.B1.GE41156

HARVESTED ON:	3/1/2017
SEQUENCED ON:	3/2/2017
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS STDTLSGQVA AADAEILLAQ PRGFCAGVDR AIEIVERAIA MHGAPIYVRH EIVHNKYVVE DLKKKGAIFV EELEEVPSGN TVIFSAHGVS KAVRDEAAVR GLRIYDATCP LVTKVHVEVA KMRQEGVDIV MIGHKGHPEV EGTMGQVERG MHLVESVEDV RRLELPDPER VALVTQTTLS VDDAAEIIGA LKAKFPAIRE PKKQDICYAT QNRQDAVKFM APQCDVVIVV GSPNSSNSSR LREVAEKRGV AAYMVDAPEQ IDPAWVAGKR RIGVTAGASA PEVLAQAVIA RLRELGVTNV RALEGIEENV SFPLPRGLNL SSAA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagct ccaccgatac gttgtccggc caggttgccg cggccgacgc cgagatcctg ctcgcccagc cgcgcggctt ctgcgcgggt gtcgaccgcg cgatcgagat cgtcgagcgt gcgattgcga tgcacggcgc gccgatctac gtgcgccacg agatcgtgca caacaagtac gtcgtcgagg atctgaagaa gaagggcgcg atttttgtcg aggaactcga ggaagtgccg tccggcaaca cggtgatctt cagcgcgcac ggcgtgtcga aggcggtgcg cgacgaggcg gccgtgcgtg ggctgcgcat ctacgatgcg acgtgcccgc tcgtcacgaa agtgcacgtc gaggtcgcga agatgcgcca ggaaggcgtc gacatcgtga tgatcggcca caaggggcat ccggaggtcg agggcacgat ggggcaggtc gagcgcggca tgcacctcgt cgagagcgtc gaggacgtgc gccggctcga gctgcccgat cccgagcgcg tcgcgctcgt cacgcagacg acgctgtccg tcgacgacgc ggccgagatc atcggcgcgt tgaaggcgaa gtttcccgcg atccgcgagc cgaagaagca ggacatctgc tacgcgacgc agaaccgcca ggacgcggtg aagttcatgg cgccgcagtg cgacgtcgtg atcgtcgtcg gcagcccgaa cagctcgaat tcgagccgcc tgcgcgaggt cgccgagaag cgcggcgtcg ccgcctacat ggtcgacgcg cccgagcaga tcgatccggc ctgggtcgcc ggcaagcgcc ggatcggcgt gaccgccggc gcgtccgcgc ccgaggtgct cgcgcaggcc gtgatcgcgc gcttgcgcga gctcggcgtg accaacgtgc gggcgctcga gggcatcgag gagaacgtgt cgttcccatt gccgcgtggc ctgaatctgt cttccgccgc ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat