BupsA.17208.e

RNA polymerase sigma-70 factor, ECF subfamily

CENTER ID: BupsA.17208.e
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.17208.e.A1.GE30952 Full length( BupsA.17208.e ) 1 173
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3960
RefSeq: YP_335174.1
UniProt: Q3JMM9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSFESDVVGW LPQLRRYARA LTGDPAWADD LVQDTVERAF SRMRAFRPDS NLRAWLLTIL RHLYIDQLRA RREFAVDDDE GPWRTLEAPA GQIDALVLRD VQRMLYRLPL EQREVLLLVC VEELSYQDAS AVLGVPVGTV MSRLSRARER MRALLTDAPL RPPLSLTAAR HHR
NT Sequence
GTGAGCTTCG AATCGGACGT CGTCGGCTGG CTGCCGCAGT TGCGCCGCTA TGCGCGCGCG CTGACGGGCG ACCCCGCGTG GGCCGACGAT CTCGTACAGG ACACCGTCGA GCGCGCGTTC TCGCGCATGC GCGCGTTCCG TCCCGACAGC AATCTGCGCG CGTGGCTGTT GACGATCCTG CGCCATCTGT ACATCGATCA GTTGCGCGCG CGCCGCGAAT TCGCCGTCGA CGACGACGAA GGCCCGTGGC GCACGCTCGA GGCGCCCGCC GGCCAGATCG ACGCGCTCGT GCTGCGCGAC GTGCAGCGAA TGCTGTACCG GCTGCCGCTC GAGCAGCGCG AAGTGCTGCT GCTCGTCTGC GTCGAAGAGT TGAGCTATCA GGACGCGTCG GCGGTGCTCG GCGTGCCGGT CGGCACCGTG ATGTCGCGGC TGTCGCGCGC GCGGGAGCGC ATGCGCGCGC TGCTGACCGA CGCGCCGCTG CGGCCGCCGC TGTCCCTTAC CGCTGCGAGA CATCATCGA
Details for BupsA.17208.e.A1.GE30952
HARVESTED ON: 11/23/2010
SEQUENCED ON: 12/6/2010
EXPECTED MW: 22kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSFESDVV GWLPQLRRYA RALTGDPAWA DDLVQDTVER AFSRMRAFRP DSNLRAWLLT ILRHLYIDQL RARREFAVDD DEGPWRTLEA PAGQIDALVL RDVQRMLYRL PLEQREVLLL VCVEELSYQD ASAVLGVPVG TVMSRLSRAR ERMRALLTDA PLRPPLSLTA AR
Validated NT Sequence
atgtctcgca gcggtaaggg acagcggcgg ccgcagcggc gcgtcggtca gcagcgcgcg catgcgctcc cgcgcgcgcg acagccgcga catcacggtg ccgaccggca cgccgagcac cgccgacgcg tcctgatagc tcaactcttc gacgcagacg agcagcagca cttcgcgctg ctcgagcggc agccggtaca gcattcgctg cacgtcgcgc agcacgagcg cgtcgatctg gccggcgggc gcctcgagcg tgcgccacgg gccttcgtcg tcgtcgacgg cgaattcgcg gcgcgcgcgc aactgatcga tgtacagatg gcgcaggatc gtcaacagcc acgcgcgcag attgctgtcg ggacggaacg cgcgcatgcg cgagaacgcg cgctcgacgg tgtcctgtac gagatcgtcg gcccacgcgg ggtcgcccgt cagcgcgcgc gcatagcggc gcaactgcgg cagccagccg acgacgtccg attcgaagct caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSFESDVV GWLPQLRRYA RALTGDPAWA DDLVQDTVER AFSRMRAFRP DSNLRAWLLT ILRHLYIDQL RARREFAVDD DEGPWRTLEA PAGQIDALVL RDVQRMLYRL PLEQREVLLL VCVEELSYQD ASAVLGVPVG TVMSRLSRAR ERMRALLTDA PLRPPLSLTA ARHHR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcttc gaatcggacg tcgtcggctg gctgccgcag ttgcgccgct atgcgcgcgc gctgacgggc gaccccgcgt gggccgacga tctcgtacag gacaccgtcg agcgcgcgtt ctcgcgcatg cgcgcgttcc gtcccgacag caatctgcgc gcgtggctgt tgacgatcct gcgccatctg tacatcgatc agttgcgcgc gcgccgcgaa ttcgccgtcg acgacgacga aggcccgtgg cgcacgctcg aggcgcccgc cggccagatc gacgcgctcg tgctgcgcga cgtgcagcga atgctgtacc ggctgccgct cgagcagcgc gaagtgctgc tgctcgtctg cgtcgaagag ttgagctatc aggacgcgtc ggcggtgctc ggcgtgccgg tcggcaccgt gatgtcgcgg ctgtcgcgcg cgcgggagcg catgcgcgcg ctgctgaccg acgcgccgct gcggccgccg ctgtccctta ccgctgcgag acatcatcga taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa