BupsA.17070.a

hypothetical protein

CENTER ID:	BupsA.17070.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.17070.a.A1.GE30687	full length		1	243

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	NO_GROUP
PATRIC ID:	fig\|320372.6.peg.5395
RefSeq:	YP_336456.1
UniProt:	Q3JIZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVQLRAHIAS LPNALRPGLP PKSRESSTHT RIGGGAKLQG KRRCKGNGRG QRTAAEPARH RSTRGPRRRR RGLSSTGVPR DATRPRPEPS AFRMSDNLCR TDLSSDAAAA VERVYREWDR AWAADDLDAM IALYAPDAVL ESPLVTHLLG TESGVVEGRE RIRALLEQAA PRKPEARMFH RIGYFTDGKR LIWEYPRATP TGEQMDFVEM MEIEHGLIRR HRVYWGWRGV SVIKADAYRK GRR

NT Sequence

atggttcaac tgcgcgctca tatcgcctcc ttgccgaatg cgcttcggcc tggtctcccg ccgaaatcgc gggaatcatc cacgcataca agaataggcg gtggcgcgaa actgcaaggg aaacggaggt gcaagggaaa cggccgcgga caacgaacgg cggctgaacc ggcgcggcac cgctcgactc gcggcccgcg ccgccggcgc cgcggacttt cttcaaccgg cgtgccccgc gacgcgacgc ggccgcgccc cgaaccatcg gcgttccgga tgagcgacaa cctctgccga accgacctgt cgagcgacgc cgccgcggca gtcgaacgcg tctaccgcga atgggaccgc gcatgggcgg ccgacgatct cgacgcgatg atcgcgctct acgcgcccga cgcggtgctc gaaagcccgc tcgtcacgca tctgctcggc accgaatcgg gcgtcgtcga agggcgcgaa cgcattcgcg cactgctcga gcaagccgcg ccgcgcaagc ccgaagcgcg catgttccat cgcatcgggt acttcaccga cggcaagcgg ctgatctggg aatacccgcg cgccacgccg accggcgaac aaatggactt cgtcgaaatg atggagatcg agcacggcct gatccggcgg catcgcgtgt actggggctg gcgcggcgtg tcggtcatca aggccgacgc gtatcgcaaa ggccgtcgat ag

Details for BupsA.17070.a.A1.GE30687

HARVESTED ON:	10/27/2010
SEQUENCED ON:	11/3/2010
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	86
PERCENT COVERAGE:	37

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVQLRAHIA SLPNALRPGL PPKSRESSTH TRIGGGAKLQ GKRRCKGNGR GQRTAAEPAR QSNASTANGT AHGRPTISTR

Validated NT Sequence

ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggttcaa ctgcgcgctc atatcgcctc cttgccgaat gcgcttcggc ctggtctccc gccgaaatcg cgggaatcat ccacgcatac aagaataggc ggtggcgcga aactgcaagg gaaacggagg tgcaagggaa acggccgcgg acaacgaacg gcggctgaac cggcgcggca gtcgaacgcg tctaccgcga atgggaccgc gcatgggcgg ccgacgatct cgacgcgatg atcgcgctct acgcgcccga cgcggtgctc gaaagcccgc tcgtcacgca tctgctcggc accgaatcgg gcgtcgtcga agggcgcgaa cgcattcgcg cactgctcga gcaagccgcg ccgcgcaagc ccgaagcgcg catgttccat cgcatcgggt acttcaccga cggcaagcgg ctgatctggg aatacccgcg cgccacgccg accggcgaac aaatggactt cgtcgaaatg atggagatcg agcacggcct gatccggcgg catcgcgtgt actggggctg gcgcggcgtg tcggtcatca aggccgacgc gtatcgcaaa ggccgtcgat aataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVQLRAHIA SLPNALRPGL PPKSRESSTH TRIGGGAKLQ GKRRCKGNGR GQRTAAEPAR HRSTRGPRRR RRGLSSTGVP RDATRPRPEP SAFRMSDNLC RTDLSSDAAA AVERVYREWD RAWAADDLDA MIALYAPDAV LESPLVTHLL GTESGVVEGR ERIRALLEQA APRKPEARMF HRIGYFTDGK RLIWEYPRAT PTGEQMDFVE MMEIEHGLIR RHRVYWGWRG VSVIKADAYR KGRR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttcaactg cgcgctcata tcgcctcctt gccgaatgcg cttcggcctg gtctcccgcc gaaatcgcgg gaatcatcca cgcatacaag aataggcggt ggcgcgaaac tgcaagggaa acggaggtgc aagggaaacg gccgcggaca acgaacggcg gctgaaccgg cgcggcaccg ctcgactcgc ggcccgcgcc gccggcgccg cggactttct tcaaccggcg tgccccgcga cgcgacgcgg ccgcgccccg aaccatcggc gttccggatg agcgacaacc tctgccgaac cgacctgtcg agcgacgccg ccgcggcagt cgaacgcgtc taccgcgaat gggaccgcgc atgggcggcc gacgatctcg acgcgatgat cgcgctctac gcgcccgacg cggtgctcga aagcccgctc gtcacgcatc tgctcggcac cgaatcgggc gtcgtcgaag ggcgcgaacg cattcgcgca ctgctcgagc aagccgcgcc gcgcaagccc gaagcgcgca tgttccatcg catcgggtac ttcaccgacg gcaagcggct gatctgggaa tacccgcgcg ccacgccgac cggcgaacaa atggacttcg tcgaaatgat ggagatcgag cacggcctga tccggcggca tcgcgtgtac tggggctggc gcggcgtgtc ggtcatcaag gccgacgcgt atcgcaaagg ccgtcgaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa