BupsA.16002.a

metallo-beta-lactamase family protein

CENTER ID:	BupsA.16002.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.16002.a.A1.GE27696	Full length( BupsA.16002.a )		1	395

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_144432
PATRIC ID:	fig\|320372.6.peg.3326
RefSeq:	YP_334562.1
UniProt:	Q3JPE1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFGAGPAHAT GAGAPLQIDV YNPGEKSLFP VSSEIVTGKT GTILIDAQFQ RNDAEALVNK IKATGKPLKL VYVSHSDPDY YFGLDTIKAA FPDAKIVATP QTVAAIKANK DDKLAYWGPI LKDNAPRTLV VPQPLHGDRL TLDGHELRIV GLDGPSPDRT FVSIPSARAV VGGIPVAANI HVWIADTQTP ESRRNWIKTL DRIAALHPSR VVPGHYLANP DGSEPYTLAS VKFTRDYLVA FDAEAAKARN SGELITAMKA RYPNLADASS LEMSAKVIKG EMQWPVVGTA SAFPAAGKKA LVQFGDIGFR LDFKDDHTMT FVGTAGQYQG ATDTVEYKAT QIRPQVFMVY WHEPKSGDNV VHVEDFENGI VYTNIAHRNG EFQHLKGTIR IEDAK

NT Sequence

ATGTTCGGCG CGGGCCCCGC ACATGCGACC GGCGCCGGCG CACCGCTCCA GATCGACGTC TACAACCCCG GCGAAAAAAG CCTCTTTCCC GTCTCGTCCG AAATCGTCAC GGGCAAGACC GGCACGATCC TCATCGACGC CCAGTTCCAG CGCAACGACG CCGAGGCGCT CGTCAACAAG ATCAAGGCGA CCGGCAAGCC GCTCAAGCTC GTCTATGTAA GCCACAGCGA TCCGGACTAT TACTTCGGCC TCGACACGAT CAAGGCGGCG TTCCCCGACG CGAAGATCGT CGCGACGCCG CAGACGGTTG CCGCGATCAA GGCGAACAAG GACGACAAGC TCGCGTACTG GGGGCCGATC CTGAAGGACA ATGCGCCGCG CACGCTCGTC GTGCCGCAGC CGCTCCACGG CGACAGGCTC ACGCTCGACG GCCATGAATT GCGCATCGTC GGCCTCGACG GCCCGTCGCC CGATCGCACG TTCGTGTCGA TCCCGTCCGC GCGCGCGGTC GTCGGCGGCA TTCCGGTGGC GGCGAACATC CACGTGTGGA TCGCCGATAC GCAAACGCCG GAGTCGCGCC GCAACTGGAT CAAGACGCTC GACCGGATCG CGGCGCTGCA CCCGTCGCGC GTCGTGCCCG GCCACTACCT CGCGAACCCG GACGGCAGCG AGCCGTACAC GCTCGCATCC GTGAAGTTCA CGCGCGACTA TCTGGTCGCG TTCGACGCGG AAGCCGCGAA AGCGAGAAAT TCCGGAGAAC TGATCACTGC AATGAAGGCG CGCTATCCGA ATCTCGCGGA TGCATCGTCG CTGGAAATGA GCGCGAAGGT CATCAAGGGC GAGATGCAAT GGCCCGTCGT CGGCACGGCC TCCGCGTTTC CGGCAGCAGG CAAGAAGGCG CTCGTGCAGT TCGGCGATAT CGGCTTTCGC CTCGACTTCA AGGACGACCA CACGATGACG TTCGTCGGCA CAGCCGGCCA GTACCAGGGC GCGACCGATA CCGTCGAATA CAAGGCAACG CAGATCCGGC CGCAGGTGTT CATGGTCTAT TGGCACGAAC CGAAGAGCGG CGACAACGTG GTGCACGTCG AAGACTTCGA GAACGGCATC GTCTATACGA ACATCGCGCA TCGCAACGGC GAGTTCCAGC ATCTGAAAGG AACGATTCGC ATCGAAGACG CGAAG

Details for BupsA.16002.a.A1.GE27696

HARVESTED ON:	10/13/2009
SEQUENCED ON:	10/21/2009
EXPECTED MW:	45kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMFGAGPAHA TGAGAPLQID VYNPGEKSLF PVSSEIVTGK TGTILIDAQF QRNDAEALVN KIKATGKPLK LVYVSHSDPD YYFGLDTIKA AFPDAKIVAT PQTVAAIKAN KDDKLAYWGP ILKDNAPRTL VVPQPLHGDR LTLDGHELRI VGLDGPSPDR TFVSIPSARA VVGGIPVAAN IHVWIADTQT PESRRNWIKT LDRIAALHPS RVVPGHYLAN PDGSEPYTLA SVKFTRDYLV AFDAEAAKAR NSGELITAMK ARYPNLADAS SLEMSAKVIK GEMQWPVVGT ASAFPAAGKK ALVQFGDIGF RLDFKDDHTM TFVGTAGQYQ GATDTVEYKA TQIRPQVFMV YWHEPKSGDN VVHVEDFENG IVYTNIAHRN GEFQHLKGTI RIE

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttcg gcgcgggccc cgcacatgcg accggcgccg gcgcaccgct ccagatcgac gtctacaacc ccggcgaaaa aagcctcttt cccgtctcgt ccgaaatcgt cacgggcaag accggcacga tcctcatcga cgcccagttc cagcgcaacg acgccgaggc gctcgtcaac aagatcaagg cgaccggcaa gccgctcaag ctcgtctatg taagccacag cgatccggac tattacttcg gcctcgacac gatcaaggcg gcgttccccg acgcgaagat cgtcgcgacg ccgcagacgg ttgccgcgat caaggcgaac aaggacgaca agctcgcgta ctgggggccg atcctgaagg acaatgcgcc gcgcacgctc gtcgtgccgc agccgctcca cggcgacagg ctcacgctcg acggccatga attgcgcatc gtcggcctcg acggcccgtc gcccgatcgc acgttcgtgt cgatcccgtc cgcgcgcgcg gtcgtcggcg gcattccggt ggcggcgaac atccacgtgt ggatcgccga tacgcaaacg ccggagtcgc gccgcaactg gatcaagacg ctcgaccgga tcgcggcgct gcacccgtcg cgcgtcgtgc ccggccacta cctcgcgaac ccggacggca gcgagccgta cacgctcgca tccgtgaagt tcacgcgcga ctatctggtc gcgttcgacg cggaagccgc gaaagcgaga aattccggag aactgatcac tgcaatgaag gcgcgctatc cgaatctcgc ggatgcatcg tcgctggaaa tgagcgcgaa ggtcatcaag ggcgagatgc aatggcccgt cgtcggcacg gcctccgcgt ttccggcagc aggcaagaag gcgctcgtgc agttcggcga tatcggcttt cgcctcgact tcaaggacga ccacacgatg acgttcgtcg gcacagccgg ccagtaccag ggcgcgaccg ataccgtcga atacaaggca acgcagatcc ggccgcaggt gttcatggtc tattggcacg aaccgaagag cggcgacaac gtggtgcacg tcgaagactt cgagaacggc atcgtctata cgaacatcgc gcatcgcaac ggcgagttcc agcatctgaa aggaacgatt cgcatcgaag

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttcggcgcg ggccccgcac atgcgaccgg cgccggcgca ccgctccaga tcgacgtcta caaccccggc gaaaaaagcc tctttcccgt ctcgtccgaa atcgtcacgg gcaagaccgg cacgatcctc atcgacgccc agttccagcg caacgacgcc gaggcgctcg tcaacaagat caaggcgacc ggcaagccgc tcaagctcgt ctatgtaagc cacagcgatc cggactatta cttcggcctc gacacgatca aggcggcgtt ccccgacgcg aagatcgtcg cgacgccgca gacggttgcc gcgatcaagg cgaacaagga cgacaagctc gcgtactggg ggccgatcct gaaggacaat gcgccgcgca cgctcgtcgt gccgcagccg ctccacggcg acaggctcac gctcgacggc catgaattgc gcatcgtcgg cctcgacggc ccgtcgcccg atcgcacgtt cgtgtcgatc ccgtccgcgc gcgcggtcgt cggcggcatt ccggtggcgg cgaacatcca cgtgtggatc gccgatacgc aaacgccgga gtcgcgccgc aactggatca agacgctcga ccggatcgcg gcgctgcacc cgtcgcgcgt cgtgcccggc cactacctcg cgaacccgga cggcagcgag ccgtacacgc tcgcatccgt gaagttcacg cgcgactatc tggtcgcgtt cgacgcggaa gccgcgaaag cgagaaattc cggagaactg atcactgcaa tgaaggcgcg ctatccgaat ctcgcggatg catcgtcgct ggaaatgagc gcgaaggtca tcaagggcga gatgcaatgg cccgtcgtcg gcacggcctc cgcgtttccg gcagcaggca agaaggcgct cgtgcagttc ggcgatatcg gctttcgcct cgacttcaag gacgaccaca cgatgacgtt cgtcggcaca gccggccagt accagggcgc gaccgatacc gtcgaataca aggcaacgca gatccggccg caggtgttca tggtctattg gcacgaaccg aagagcggcg acaacgtggt gcacgtcgaa gacttcgaga acggcatcgt ctatacgaac atcgcgcatc gcaacggcga gttccagcat ctgaaaggaa cgattcgcat cgaagacgcg aagaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa