BupsA.16001.a

metallo-beta-lactamase family protein

CENTER ID:	BupsA.16001.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.16001.a.A1.GE27695	Full length( BupsA.16001.a )		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_140535
PATRIC ID:	fig\|320372.6.peg.2825
RefSeq:	YP_334082.1
UniProt:	Q3JQS1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRFASLGSGS EGNALVVEAS NGATTTRVLL DCGFSAKELE RRLARIDLRV DDLDAILVTH EHGDHVGCAL TLARRASLPL YTSWGTARAV GADEADVDLH VLWSDETAAV GDLCVLPYTV PHDAREPLQF VFSDGATRLG VLTDVGMSTP HIGAVLSGCD GLVLEANHDV AMLAASRYPA SLKARIGGSH GHLSNDAAAA ILAELDRSRL RHLVAAHLSQ QNNAPALAQA ALAAALGGQA DDVLVASQDD GFGWLSLS

NT Sequence

GTGCGATTCG CGAGCCTCGG AAGCGGCAGC GAAGGCAATG CGCTCGTCGT CGAAGCATCG AACGGCGCGA CGACGACACG TGTGCTGCTC GATTGCGGTT TCTCGGCGAA GGAACTCGAA CGCCGGCTCG CGCGCATCGA TTTGCGCGTC GACGATCTCG ACGCGATCCT CGTTACCCAC GAACACGGCG ATCATGTCGG CTGCGCGCTG ACGCTCGCGC GTCGCGCGTC GCTGCCGCTG TACACGAGCT GGGGCACCGC GCGCGCGGTC GGCGCGGACG AGGCCGACGT CGACCTCCAT GTGCTCTGGA GCGACGAGAC GGCCGCGGTG GGCGATCTGT GCGTGCTGCC CTATACCGTC CCGCACGATG CGCGCGAGCC GCTCCAGTTC GTCTTCAGCG ACGGCGCGAC GCGTCTCGGC GTGCTGACCG ACGTCGGCAT GTCGACGCCG CACATCGGCG CCGTGCTGAG CGGCTGCGAC GGCTTGGTGC TGGAGGCCAA CCACGATGTC GCGATGCTCG CGGCGAGCCG CTATCCGGCG TCGCTGAAGG CGCGCATCGG CGGCTCGCAC GGCCATCTGA GCAACGACGC GGCGGCGGCC ATTCTCGCCG AGCTCGATCG CAGCCGGCTG CGGCACCTGG TGGCTGCCCA TCTGAGCCAG CAGAACAATG CGCCCGCGCT CGCGCAGGCG GCGCTCGCGG CGGCCCTGGG CGGGCAGGCG GACGACGTGC TCGTCGCATC GCAGGACGAC GGATTCGGCT GGCTCAGCCT GAGC

Details for BupsA.16001.a.A1.GE27695

HARVESTED ON:	10/13/2009
SEQUENCED ON:	10/21/2009
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVRFASLGS GSEGNALVVE ASNGATTTRV LLDCGFSAKE LERRLARIDL RVDDLDAILV THEHGDHVGC ALTLARRASL PLYTSWGTAR AVGADEADVD LHVLWSDETA AVGDLCVLPY TVPHDAREPL QFVFSDGATR LGVLTDVGMS TPHIGAVLSG CDGLVLEANH DVAMLAASRY PASLKARIGG SHGHLSNDAA AAILAELDRS RLRHLVAAHL SQQNNAPALA QAALAAALGG QADDVLVASQ DDGFGWLSLS

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc gattcgcgag cctcggaagc ggcagcgaag gcaatgcgct cgtcgtcgaa gcatcgaacg gcgcgacgac gacacgtgtg ctgctcgatt gcggtttctc ggcgaaggaa ctcgaacgcc ggctcgcgcg catcgatttg cgcgtcgacg atctcgacgc gatcctcgtt acccacgaac acggcgatca tgtcggctgc gcgctgacgc tcgcgcgtcg cgcgtcgctg ccgctgtaca cgagctgggg caccgcgcgc gcggtcggcg cggacgaggc cgacgtcgac ctccatgtgc tctggagcga cgagacggcc gcggtgggcg atctgtgcgt gctgccctat accgtcccgc acgatgcgcg cgagccgctc cagttcgtct tcagcgacgg cgcgacgcgt ctcggcgtgc tgaccgacgt cggcatgtcg acgccgcaca tcggcgccgt gctgagcggc tgcgacggct tggtgctgga ggccaaccac gatgtcgcga tgctcgcggc gagccgctat ccggcgtcgc tgaaggcgcg catcggcggc tcgcacggcc atctgagcaa cgacgcggcg gcggccattc tcgccgagct cgatcgcagc cggctgcggc acctggtggc tgcccatctg agccagcaga acaatgcgcc cgcgctcgcg caggcggcgc tcgcggcggc cctgggcggg caggcggacg acgtgctcgt cgcatcgcag gacgacggat tcggctggct cagcctgagc taataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgattc gcgagcctcg gaagcggcag cgaaggcaat gcgctcgtcg tcgaagcatc gaacggcgcg acgacgacac gtgtgctgct cgattgcggt ttctcggcga aggaactcga acgccggctc gcgcgcatcg atttgcgcgt cgacgatctc gacgcgatcc tcgttaccca cgaacacggc gatcatgtcg gctgcgcgct gacgctcgcg cgtcgcgcgt cgctgccgct gtacacgagc tggggcaccg cgcgcgcggt cggcgcggac gaggccgacg tcgacctcca tgtgctctgg agcgacgaga cggccgcggt gggcgatctg tgcgtgctgc cctataccgt cccgcacgat gcgcgcgagc cgctccagtt cgtcttcagc gacggcgcga cgcgtctcgg cgtgctgacc gacgtcggca tgtcgacgcc gcacatcggc gccgtgctga gcggctgcga cggcttggtg ctggaggcca accacgatgt cgcgatgctc gcggcgagcc gctatccggc gtcgctgaag gcgcgcatcg gcggctcgca cggccatctg agcaacgacg cggcggcggc cattctcgcc gagctcgatc gcagccggct gcggcacctg gtggctgccc atctgagcca gcagaacaat gcgcccgcgc tcgcgcaggc ggcgctcgcg gcggccctgg gcgggcaggc ggacgacgtg ctcgtcgcat cgcaggacga cggattcggc tggctcagcc tgagcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa