BupsA.15998.a

DNA polymerase III, alpha subunit, form 1

CENTER ID: BupsA.15998.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.15998.a.A1.GE27692 Full length( BupsA.15998.a ) 1 389
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.1391
RefSeq: YP_332766.1
UniProt: Q3JUI7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDDTDKVKIL FDDCLVNGLA VLPPDINRSN YRFEPVAEAD GKRSRTIRYG LGAIKGSGQN AIEEILRARE EKPFADLFDF CERIDRRVVN RRTIEAMIRA GAFDSLHENR AQLLASVPLA MEAAEQAAAN ALQAGLFDIG GVPAHQHALV DEPAWDDKRR LQEEKGALGF YLSGHLFDAY RDEVRRFVRQ KLGELKEGRD KVVAGVIASL RTQMTQRGKM VIALLDDGTG QCEVTVFNEQ FDANRALFKE DELLIVQGQA RNDAFTGGIR FTAESVMDLE RARSRYAQAV RMTMNGNADA AALRRVLEAH VAKPDETPPA AAPAPRGGRD GGRRAQAAIP NGLAVRIAYS NARAQGEMRL GDAWRVKPSD ALLADLRAAF GGSVVEIVY
NT Sequence
ATGGACGACA CCGACAAGGT GAAGATCCTG TTCGACGATT GCCTCGTCAA CGGCCTCGCC GTGCTGCCGC CCGACATCAA CCGTTCGAAC TATCGCTTCG AGCCCGTCGC CGAGGCCGAC GGCAAACGCT CGCGCACGAT CCGCTACGGC CTCGGCGCGA TCAAGGGCAG CGGCCAGAAC GCGATCGAGG AGATCCTGCG CGCACGCGAG GAAAAGCCGT TCGCCGATCT GTTCGATTTC TGCGAGCGGA TCGACCGCCG CGTCGTGAAC CGCCGCACGA TCGAAGCGAT GATTCGCGCG GGCGCATTCG ATTCGCTGCA CGAGAATCGC GCGCAGTTGC TCGCATCGGT GCCGCTCGCG ATGGAGGCCG CCGAGCAGGC GGCCGCGAAC GCGCTGCAGG CGGGCCTGTT CGACATCGGC GGCGTGCCCG CGCACCAGCA TGCGCTCGTC GACGAGCCGG CGTGGGACGA CAAGCGTCGC CTGCAGGAAG AGAAGGGCGC GCTCGGCTTC TACCTGTCCG GCCACCTGTT CGACGCGTAT CGCGACGAGG TGCGCCGTTT CGTGCGCCAG AAGCTCGGCG AGCTGAAGGA AGGGCGCGAC AAGGTGGTGG CCGGCGTGAT CGCGTCGTTG CGCACGCAGA TGACGCAGCG CGGCAAGATG GTGATCGCGT TGCTCGACGA CGGCACCGGC CAGTGCGAAG TCACCGTGTT CAACGAGCAG TTCGACGCGA ACCGCGCGCT CTTCAAGGAG GACGAGCTGC TGATCGTCCA GGGGCAGGCG CGCAACGACG CGTTCACGGG CGGAATTCGC TTCACCGCCG AGTCGGTGAT GGACCTCGAG CGTGCGCGCA GCCGCTACGC GCAGGCGGTG CGGATGACGA TGAACGGCAA CGCGGACGCG GCGGCGCTGC GCCGCGTGCT GGAAGCGCAC GTCGCGAAAC CCGACGAGAC GCCGCCCGCC GCGGCGCCGG CGCCGCGCGG CGGCCGCGAC GGCGGGCGGC GGGCGCAGGC GGCGATACCG AATGGTCTCG CCGTGCGGAT CGCATACAGC AACGCGCGTG CGCAAGGCGA GATGCGCCTG GGCGACGCAT GGCGCGTGAA GCCGAGCGAC GCGTTGCTCG CCGATCTGCG CGCGGCGTTC GGCGGCAGCG TCGTCGAGAT CGTCTAC
Details for BupsA.15998.a.A1.GE27692
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 75
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDDTDKVKI LFDDCLVNGL AVLPPDINRS NYRFEPVAEA DGKRSRTIRY GLGAIKGSGQ NAIEEILRAR EEKPFADLFD FCERIDRRVV NRRTIEAMIR AGAFDSLHEN RAQLLASVPL AMEAAEQAAA NALQAGLFDI GGVPAHQHAL VDEPAWDDKR RLQEEKGALG FYLSGHLFDA YRDEVRRFXR QXLGELKEXR DKVVAGVIAS LRTXMTXAXR XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXEXPP AXAXAPRGGX XGGRRAQAAI PNGLAVRIAY SNARAQGEMR LGDAWRVKPS DALLADLRAA FGGSVVEIVY
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDDTDKVKI LFDDCLVNGL AVLPPDINRS NYRFEPVAEA DGKRSRTIRY GLGAIKGSGQ NAIEEILRAR EEKPFADLFD FCERIDRRVV NRRTIEAMIR AGAFDSLHEN RAQLLASVPL AMEAAEQAAA NALQAGLFDI GGVPAHQHAL VDEPAWDDKR RLQEEKGALG FYLSGHLFDA YRDEVRRFVR QKLGELKEGR DKVVAGVIAS LRTQMTQRGK MVIALLDDGT GQCEVTVFNE QFDANRALFK EDELLIVQGQ ARNDAFTGGI RFTAESVMDL ERARSRYAQA VRMTMNGNAD AAALRRVLEA HVAKPDETPP AAAPAPRGGR DGGRRAQAAI PNGLAVRIAY SNARAQGEMR LGDAWRVKPS DALLADLRAA FGGSVVEIVY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacgacacc gacaaggtga agatcctgtt cgacgattgc ctcgtcaacg gcctcgccgt gctgccgccc gacatcaacc gttcgaacta tcgcttcgag cccgtcgccg aggccgacgg caaacgctcg cgcacgatcc gctacggcct cggcgcgatc aagggcagcg gccagaacgc gatcgaggag atcctgcgcg cacgcgagga aaagccgttc gccgatctgt tcgatttctg cgagcggatc gaccgccgcg tcgtgaaccg ccgcacgatc gaagcgatga ttcgcgcggg cgcattcgat tcgctgcacg agaatcgcgc gcagttgctc gcatcggtgc cgctcgcgat ggaggccgcc gagcaggcgg ccgcgaacgc gctgcaggcg ggcctgttcg acatcggcgg cgtgcccgcg caccagcatg cgctcgtcga cgagccggcg tgggacgaca agcgtcgcct gcaggaagag aagggcgcgc tcggcttcta cctgtccggc cacctgttcg acgcgtatcg cgacgaggtg cgccgtttcg tgcgccagaa gctcggcgag ctgaaggaag ggcgcgacaa ggtggtggcc ggcgtgatcg cgtcgttgcg cacgcagatg acgcagcgcg gcaagatggt gatcgcgttg ctcgacgacg gcaccggcca gtgcgaagtc accgtgttca acgagcagtt cgacgcgaac cgcgcgctct tcaaggagga cgagctgctg atcgtccagg ggcaggcgcg caacgacgcg ttcacgggcg gaattcgctt caccgccgag tcggtgatgg acctcgagcg tgcgcgcagc cgctacgcgc aggcggtgcg gatgacgatg aacggcaacg cggacgcggc ggcgctgcgc cgcgtgctgg aagcgcacgt cgcgaaaccc gacgagacgc cgcccgccgc ggcgccggcg ccgcgcggcg gccgcgacgg cgggcggcgg gcgcaggcgg cgataccgaa tggtctcgcc gtgcggatcg catacagcaa cgcgcgtgcg caaggcgaga tgcgcctggg cgacgcatgg cgcgtgaagc cgagcgacgc gttgctcgcc gatctgcgcg cggcgttcgg cggcagcgtc gtcgagatcg tctacaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa