SSGCID: Target Page

BupsA.12800.d

Oxidative stress regulatory protein

CENTER ID:	BupsA.12800.d
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.12800.d.A1.GE30943	Full length( BupsA.12800.d )		1	319

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.12800.d.A1.PW30831	Full length( BupsA.12800.d )		1	319

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.3504
RefSeq:	YP_334738.1
UniProt:	Q3JNW5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTLTELKYIV AVARERHFGR AAEACFVSQP TLSVAIKKLE DELNVQIFER GASEVSVTPV GDQIVTQAQR VLEQTFAIKE IAKQGKDPLI GPFRLGVIYT IGPYLLPTLV KQMIRRVPQM PLMLQENYTL KLLELLKQGE IDAAIMALPF PETGLMVRAL YDEPFVVALP AGHPWEKRRK IDAADLKQET MLLLGNGHCF RDHVLGVCPE LMHFSQTADG IQKTFEGSSL ETIRHMVASG VGITVLPRMS VAEVGPHASG PDADLLSYVP FEDPVPDRRV VLVWRKSFTR MPAIDAISEA IAACGLPGVT KLDIPATVN

NT Sequence

ATGACGCTTA CCGAACTGAA GTACATCGTC GCGGTCGCGC GCGAACGCCA TTTCGGCCGG GCGGCCGAAG CCTGCTTCGT CAGCCAGCCG ACGCTGTCGG TCGCGATCAA GAAGCTGGAA GACGAGTTGA ACGTGCAGAT CTTCGAGCGC GGCGCGAGCG AAGTGAGCGT GACGCCGGTC GGCGACCAGA TCGTCACGCA AGCGCAGCGC GTGCTCGAGC AGACGTTCGC GATCAAGGAG ATCGCGAAGC AGGGCAAGGA TCCGCTCATC GGGCCGTTCC GGCTCGGCGT GATCTACACG ATCGGGCCCT ATCTGCTGCC GACGCTCGTC AAGCAGATGA TCCGCCGCGT GCCGCAAATG CCGCTGATGC TGCAGGAGAA CTACACGCTG AAGCTGCTCG AGCTGCTCAA GCAAGGCGAG ATCGACGCGG CGATCATGGC GTTGCCGTTT CCCGAAACCG GCCTGATGGT GCGGGCGCTG TACGACGAGC CGTTCGTCGT CGCGCTGCCC GCCGGGCATC CGTGGGAGAA GCGCCGCAAG ATCGACGCGG CGGACCTGAA GCAGGAGACG ATGCTGCTGC TCGGCAATGG CCACTGCTTC CGCGATCATG TGCTCGGCGT GTGCCCGGAG CTGATGCACT TCTCGCAGAC GGCGGACGGC ATCCAGAAGA CGTTCGAGGG CTCGTCGCTC GAGACGATCC GCCATATGGT CGCGAGCGGC GTCGGGATCA CGGTGCTGCC CCGGATGTCG GTCGCCGAGG TGGGGCCGCA TGCGAGCGGG CCCGATGCTG ACTTGCTGTC GTACGTGCCG TTCGAGGATC CGGTGCCGGA TCGGCGCGTC GTGCTCGTGT GGCGCAAGAG CTTCACGCGG ATGCCGGCGA TCGACGCGAT CAGCGAGGCG ATCGCCGCGT GCGGGCTGCC CGGGGTGACG AAGCTCGATA TCCCGGCGAC GGTGAAT

Details for BupsA.12800.d.A1.GE30943

HARVESTED ON:	11/23/2010
SEQUENCED ON:	12/6/2010
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMTLTELK YIVAVARERH FGRAAEACFV SQPTLSVAIK KLEDELNVQI FERGASEVSV TPVGDQIVTQ AQRVLEQTFA IKEIAKQGKD PLIGPFRLGV IYTIGPYLLP TLVKQMIRRV PQMPLMLQEN YTLKLLELLK QGEIDAAIMA LPFPETGLMV RALYDEPFVV ALPAGHPWEK RRKIDAADLK QETMLLLGNG HCFRDHVLGV CPELMHFSQT ADGIQKTFEG SSLETIRHMV ASGVGITVLP RMSVAEVGPH ASGPDADLLS YVPFEDPVPD RRVVLVWRKS FTRMPAIDAI SEAIAACGLP GVTKLDIPAT VN

Validated NT Sequence

aattcaccgt cgccgggata tcgagcttcg tcaccccggg cagcccgcac gcggcgatcg cctcgctgat cgcgtcgatc gccggcatcc gcgtgaagct cttgcgccac acgagcacga cgcgccgatc cggcaccgga tcctcgaacg gcacgtacga cagcaagtca gcatcgggcc cgctcgcatg cggccccacc tcggcgaccg acatccgggg cagcaccgtg atcccgacgc cgctcgcgac catatggcgg atcgtctcga gcgacgagcc ctcgaacgtc ttctggatgc cgtccgccgt ctgcgagaag tgcatcagct ccgggcacac gccgagcaca tgatcgcgga agcagtggcc attgccgagc agcagcatcg tctcctgctt caggtccgcc gcgtcgatct tgcggcgctt ctcccacgga tgcccggcgg gcagcgcgac gacgaacggc tcgtcgtaca gcgcccgcac catcaggccg gtttcgggaa acggcaacgc catgatcgcc gcgtcgatct cgccttgctt gagcagctcg agcagcttca gcgtgtagtt ctcctgcagc atcagcggca tttgcggcac gcggcggatc atctgcttga cgagcgtcgg cagcagatag ggcccgatcg tgtagatcac gccgagccgg aacggcccga tgagcggatc cttgccctgc ttcgcgatct ccttgatcgc gaacgtctgc tcgagcacgc gctgcgcttg cgtgacgatc tggtcgccga ccggcgtcac gctcacttcg ctcgcgccgc gctcgaagat ctgcacgttc aactcgtctt ccagcttctt gatcgcgacc gacagcgtcg gctggctgac gaagcaggct tcggccgccc ggccgaaatg gcgttcgcgc gcgaccgcga cgatgtactt cagttcggta agcgtcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTLTELKYI VAVARERHFG RAAEACFVSQ PTLSVAIKKL EDELNVQIFE RGASEVSVTP VGDQIVTQAQ RVLEQTFAIK EIAKQGKDPL IGPFRLGVIY TIGPYLLPTL VKQMIRRVPQ MPLMLQENYT LKLLELLKQG EIDAAIMALP FPETGLMVRA LYDEPFVVAL PAGHPWEKRR KIDAADLKQE TMLLLGNGHC FRDHVLGVCP ELMHFSQTAD GIQKTFEGSS LETIRHMVAS GVGITVLPRM SVAEVGPHAS GPDADLLSYV PFEDPVPDRR VVLVWRKSFT RMPAIDAISE AIAACGLPGV TKLDIPATVN

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcttacc gaactgaagt acatcgtcgc ggtcgcgcgc gaacgccatt tcggccgggc ggccgaagcc tgcttcgtca gccagccgac gctgtcggtc gcgatcaaga agctggaaga cgagttgaac gtgcagatct tcgagcgcgg cgcgagcgaa gtgagcgtga cgccggtcgg cgaccagatc gtcacgcaag cgcagcgcgt gctcgagcag acgttcgcga tcaaggagat cgcgaagcag ggcaaggatc cgctcatcgg gccgttccgg ctcggcgtga tctacacgat cgggccctat ctgctgccga cgctcgtcaa gcagatgatc cgccgcgtgc cgcaaatgcc gctgatgctg caggagaact acacgctgaa gctgctcgag ctgctcaagc aaggcgagat cgacgcggcg atcatggcgt tgccgtttcc cgaaaccggc ctgatggtgc gggcgctgta cgacgagccg ttcgtcgtcg cgctgcccgc cgggcatccg tgggagaagc gccgcaagat cgacgcggcg gacctgaagc aggagacgat gctgctgctc ggcaatggcc actgcttccg cgatcatgtg ctcggcgtgt gcccggagct gatgcacttc tcgcagacgg cggacggcat ccagaagacg ttcgagggct cgtcgctcga gacgatccgc catatggtcg cgagcggcgt cgggatcacg gtgctgcccc ggatgtcggt cgccgaggtg gggccgcatg cgagcgggcc cgatgctgac ttgctgtcgt acgtgccgtt cgaggatccg gtgccggatc ggcgcgtcgt gctcgtgtgg cgcaagagct tcacgcggat gccggcgatc gacgcgatca gcgaggcgat cgccgcgtgc gggctgcccg gggtgacgaa gctcgatatc ccggcgacgg tgaattaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BupsA.12800.d.A1.PW30831

PURIFICATION DATe:	2/25/2011
CONCENTRATION:	20.61mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGCTTACC GAACTGAAGT ACATCGTCGC GGTCGCGCGC GAACGCCATT TCGGCCGGGC GGCCGAAGCC TGCTTCGTCA GCCAGCCGAC GCTGTCGGTC GCGATCAAGA AGCTGGAAGA CGAGTTGAAC GTGCAGATCT TCGAGCGCGG CGCGAGCGAA GTGAGCGTGA CGCCGGTCGG CGACCAGATC GTCACGCAAG CGCAGCGCGT GCTCGAGCAG ACGTTCGCGA TCAAGGAGAT CGCGAAGCAG GGCAAGGATC CGCTCATCGG GCCGTTCCGG CTCGGCGTGA TCTACACGAT CGGGCCCTAT CTGCTGCCGA CGCTCGTCAA GCAGATGATC CGCCGCGTGC CGCAAATGCC GCTGATGCTG CAGGAGAACT ACACGCTGAA GCTGCTCGAG CTGCTCAAGC AAGGCGAGAT CGACGCGGCG ATCATGGCGT TGCCGTTTCC CGAAACCGGC CTGATGGTGC GGGCGCTGTA CGACGAGCCG TTCGTCGTCG CGCTGCCCGC CGGGCATCCG TGGGAGAAGC GCCGCAAGAT CGACGCGGCG GACCTGAAGC AGGAGACGAT GCTGCTGCTC GGCAATGGCC ACTGCTTCCG CGATCATGTG CTCGGCGTGT GCCCGGAGCT GATGCACTTC TCGCAGACGG CGGACGGCAT CCAGAAGACG TTCGAGGGCT CGTCGCTCGA GACGATCCGC CATATGGTCG CGAGCGGCGT CGGGATCACG GTGCTGCCCC GGATGTCGGT CGCCGAGGTG GGGCCGCATG CGAGCGGGCC CGATGCTGAC TTGCTGTCGT ACGTGCCGTT CGAGGATCCG GTGCCGGATC GGCGCGTCGT GCTCGTGTGG CGCAAGAGCT TCACGCGGAT GCCGGCGATC GACGCGATCA GCGAGGCGAT CGCCGCGTGC GGGCTGCCCG GGGTGACGAA GCTCGATATC CCGGCGACGG TGAATTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA