SSGCID: Target Page

BupsA.12800.a

transcriptional regulator, LysR family

CENTER ID:	BupsA.12800.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.12800.a.A2.GE27685	Mature protein( BupsA.12800.a )		21	299

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.198
RefSeq:	YP_331619.1
UniProt:	Q3JXT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLTLLRAFV AVAREGNLTR AAAQLHLTQP AVSLQIKNLQ DLLGIALFVR TSRGLVLTRD GQALLPHAER ALDAAADVKR AAAALRHEVR GRLRIGTILD PEFLRLGGFL KRLVEAHPQI ETALRHGMSG WVLDEVRARE LDVGYYIGRP EEDDPRDAER FHTVTLTQFR YRVLAPAGWK ERVQRARTWR ELAALPWIWT PAASAHQRLL SRRFADAGAQ PVKVAEVDQE TSMLDLVKSG VGLTLARDSI ALREAHAHAL TIVEHVAVPA ELTFVTLADR RDEPAIAAAL RLIDEQWAI

NT Sequence

ATGGACCTGA CTCTGCTCCG CGCGTTCGTC GCGGTCGCGC GCGAGGGCAA CCTCACGCGC GCGGCGGCGC AACTGCATCT GACGCAGCCC GCCGTCAGCC TGCAGATCAA GAATCTGCAG GATCTGCTCG GCATCGCGCT GTTCGTGCGC ACGTCGCGCG GGCTCGTGCT CACGCGCGAC GGCCAGGCGC TGCTGCCGCA CGCGGAACGC GCGCTCGACG CGGCGGCCGA CGTGAAGCGC GCGGCCGCCG CGCTCAGGCA CGAAGTGCGC GGCAGGCTGC GGATCGGCAC GATTCTCGAC CCCGAGTTCC TGCGGCTGGG CGGCTTCCTG AAGCGGCTCG TCGAAGCGCA TCCGCAGATC GAGACGGCGC TGCGGCACGG CATGTCCGGC TGGGTGCTCG ACGAGGTCCG CGCGCGCGAG CTCGACGTCG GCTATTACAT CGGCCGGCCG GAGGAGGACG ATCCGCGCGA CGCCGAGCGC TTTCACACGG TGACGCTCAC GCAGTTCCGC TACCGGGTGC TCGCGCCCGC CGGCTGGAAG GAGCGCGTGC AGCGCGCGCG CACGTGGCGC GAGCTCGCGG CACTGCCGTG GATCTGGACG CCCGCCGCAT CCGCGCACCA GCGCCTCCTG TCGCGCCGCT TCGCCGATGC GGGCGCGCAG CCCGTGAAGG TCGCCGAGGT CGATCAGGAG ACGTCGATGC TCGATCTCGT CAAATCGGGA GTCGGCCTGA CACTCGCGCG CGATTCGATC GCGCTGCGGG AGGCGCACGC GCACGCGCTC ACGATCGTCG AGCATGTCGC GGTGCCCGCC GAGCTCACGT TCGTCACGCT CGCCGATCGC CGCGACGAAC CGGCGATCGC CGCCGCGCTT CGCCTCATCG ACGAGCAATG GGCGATA

Details for BupsA.12800.a.A2.GE27685

HARVESTED ON:	10/13/2009
SEQUENCED ON:	10/21/2009
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

MAAAQLHLTQ PAVSLQIKNL QDLLGIALFV RTSRGLVLTR DGQALLPHAE RALDAAADVK RAAAALRHEV RGRLRIGTIL DPEFLRLGGF LKRLVEAHPQ IETALRHGMS GWVLDEVRAR ELDVGYYIGR PEEDDPRDAE RFHTVTLTQF RYRVLAPAGW KERVQRARTW RELAALPWIW TPAASAHQRL LSRRFADAGA QPVKVAEVDQ ETSMLDLVKS GVGLTLARDS IALREAHAHA LTIVEHVAVP AELTFVTLAD RRDEPAIAAA LRLIDEQWAI

Validated NT Sequence

tatatcgccc attgctcgtc gatgaggcga agcgcggcgg cgatcgccgg ttcgtcgcgg cgatcggcga gcgtgacgaa cgtgagctcg gcgggcaccg cgacatgctc gacgatcgtg agcgcgtgcg cgtgcgcctc ccgcagcgcg atcgaatcgc gcgcgagtgt caggccgact cccgatttga cgagatcgag catcgacgtc tcctgatcga cctcggcgac cttcacgggc tgcgcgcccg catcggcgaa gcggcgcgac aggaggcgct ggtgcgcgga tgcggcgggc gtccagatcc acggcagtgc cgcgagctcg cgccacgtgc gcgcgcgctg cacgcgctcc ttccagccgg cgggcgcgag cacccggtag cggaactgcg tgagcgtcac cgtgtgaaag cgctcggcgt cgcgcggatc gtcctcctcc ggccggccga tgtaatagcc gacgtcgagc tcgcgcgcgc ggacctcgtc gagcacccag ccggacatgc cgtgccgcag cgccgtctcg atctgcggat gcgcttcgac gagccgcttc aggaagccgc ccagccgcag gaactcgggg tcgagaatcg tgccgatccg cagcctgccg cgcacttcgt gcctgagcgc ggcggccgcg cgcttcacgt cggccgccgc gtcgagcgcg cgttccgcgt gcggcagcag cgcctggccg tcgcgcgtga gcacgagccc gcgcgacgtg cgcacgaaca gcgcgatgcc gagcagatcc tgcagattct tgatctgcag gctgacggcg ggctgcgtca gatgcagttg cgccgccgcc atcgaaccag gaccctggg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAAAQLHLT QPAVSLQIKN LQDLLGIALF VRTSRGLVLT RDGQALLPHA ERALDAAADV KRAAAALRHE VRGRLRIGTI LDPEFLRLGG FLKRLVEAHP QIETALRHGM SGWVLDEVRA RELDVGYYIG RPEEDDPRDA ERFHTVTLTQ FRYRVLAPAG WKERVQRART WRELAALPWI WTPAASAHQR LLSRRFADAG AQPVKVAEVD QETSMLDLVK SGVGLTLARD SIALREAHAH ALTIVEHVAV PAELTFVTLA DRRDEPAIAA ALRLIDEQWA I

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggcggcg caactgcatc tgacgcagcc cgccgtcagc ctgcagatca agaatctgca ggatctgctc ggcatcgcgc tgttcgtgcg cacgtcgcgc gggctcgtgc tcacgcgcga cggccaggcg ctgctgccgc acgcggaacg cgcgctcgac gcggcggccg acgtgaagcg cgcggccgcc gcgctcaggc acgaagtgcg cggcaggctg cggatcggca cgattctcga ccccgagttc ctgcggctgg gcggcttcct gaagcggctc gtcgaagcgc atccgcagat cgagacggcg ctgcggcacg gcatgtccgg ctgggtgctc gacgaggtcc gcgcgcgcga gctcgacgtc ggctattaca tcggccggcc ggaggaggac gatccgcgcg acgccgagcg ctttcacacg gtgacgctca cgcagttccg ctaccgggtg ctcgcgcccg ccggctggaa ggagcgcgtg cagcgcgcgc gcacgtggcg cgagctcgcg gcactgccgt ggatctggac gcccgccgca tccgcgcacc agcgcctcct gtcgcgccgc ttcgccgatg cgggcgcgca gcccgtgaag gtcgccgagg tcgatcagga gacgtcgatg ctcgatctcg tcaaatcggg agtcggcctg acactcgcgc gcgattcgat cgcgctgcgg gaggcgcacg cgcacgcgct cacgatcgtc gagcatgtcg cggtgcccgc cgagctcacg ttcgtcacgc tcgccgatcg ccgcgacgaa ccggcgatcg ccgccgcgct tcgcctcatc gacgagcaat gggcgataaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa