BupsA.12707.a

squalene/phytoene synthase

CENTER ID: BupsA.12707.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.12707.a.A1.GE27682 Full length( BupsA.12707.a ) 1 275
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.12707.a.A1.PW27545 Full length( BupsA.12707.a ) 1 275

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.5600
RefSeq: YP_336648.1
UniProt: Q3JIF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDDNETDAAA VTSGSSFYLA MRILPAEQRD AMFQVYAFCR AVDDIADEGG ARAERAAQLD RWRADIDDCY AGKPRASLVP LAREIGKFGL HRDDFHAMID GMAMDAAEDI CAPDEATLDL YCDRVASSAG RLSVRIFGMP DEPGRVLSHH LGRALQLTNI LRDIDDDAAI NRCYLPRELL AREGIAIADP QTIARDPLLP RVCATLVERA LHHFAQADAV MDASPRAQVR APRIMSGAYR LILEAAVARG FAPPRAPLRK PRARMLLLAA RYALF
NT Sequence
GTGGACGATA ACGAAACCGA CGCCGCTGCC GTCACATCGG GCAGCTCTTT CTATCTCGCG ATGCGCATCC TGCCCGCGGA GCAGCGCGAT GCGATGTTCC AGGTCTATGC GTTTTGCCGT GCGGTCGACG ACATCGCCGA CGAAGGCGGC GCGCGCGCGG AGCGCGCGGC GCAGCTCGAC CGCTGGCGCG CCGACATCGA TGATTGCTAC GCGGGCAAGC CGCGCGCGTC GCTCGTGCCG CTCGCGCGCG AGATCGGCAA GTTCGGCCTG CATCGCGACG ACTTTCACGC GATGATCGAC GGCATGGCGA TGGACGCCGC CGAGGACATC TGCGCGCCCG ACGAAGCCAC GCTCGATCTG TACTGCGACC GCGTCGCGAG CTCGGCCGGC CGGCTATCGG TGAGGATTTT CGGGATGCCG GACGAGCCCG GCCGCGTACT GTCGCATCAT CTCGGCCGCG CGCTGCAACT GACGAATATC CTGCGCGACA TCGACGATGA CGCGGCGATC AACCGTTGCT ACCTGCCGCG CGAGCTGCTC GCGCGCGAGG GCATCGCGAT CGCCGATCCG CAGACGATCG CGCGCGATCC GTTGCTGCCG CGCGTGTGCG CGACGCTCGT CGAGCGCGCA CTGCACCATT TCGCGCAGGC CGACGCGGTG ATGGACGCGT CGCCGCGCGC GCAGGTTCGT GCGCCGCGCA TCATGTCGGG CGCGTATCGC CTGATTCTCG AAGCCGCGGT CGCGCGCGGC TTCGCGCCGC CGCGCGCGCC GCTGCGCAAG CCGCGCGCGC GAATGCTGCT GCTCGCGGCG CGTTACGCGC TGTTT
Details for BupsA.12707.a.A1.GE27682
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 32kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDDNETDA AAVTSGSSFY LAMRILPAEQ RDAMFQVYAF CRAVDDIADE GGARAERAAQ LDRWRADIDD CYAGKPRASL VPLAREIGKF GLHRDDFHAM IDGMAMDAAE DICAPDEATL DLYCDRVASS AGRLSVRIFG MPDEPGRVLS HHLGRALQLT NILRDIDDDA AINRCYLPRE LLAREGIAIA DPQTIARDPL LPRVCATLVE RALHHFAQAD AVMDASPRAQ VRAPRIMSGA YRLILEAAVA RGFAPPRAPL RKPRARMLLL AARYALF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg acgataacga aaccgacgcc gctgccgtca catcgggcag ctctttctat ctcgcgatgc gcatcctgcc cgcggagcag cgcgatgcga tgttccaggt ctatgcgttt tgccgtgcgg tcgacgacat cgccgacgaa ggcggcgcgc gcgcggagcg cgcggcgcag ctcgaccgct ggcgcgccga catcgatgat tgctacgcgg gcaagccgcg cgcgtcgctc gtgccgctcg cgcgcgagat cggcaagttc ggcctgcatc gcgacgactt tcacgcgatg atcgacggca tggcgatgga cgccgccgag gacatctgcg cgcccgacga agccacgctc gatctgtact gcgaccgcgt cgcgagctcg gccggccggc tatcggtgag gattttcggg atgccggacg agcccggccg cgtactgtcg catcatctcg gccgcgcgct gcaactgacg aatatcctgc gcgacatcga cgatgacgcg gcgatcaacc gttgctacct gccgcgcgag ctgctcgcgc gcgagggcat cgcgatcgcc gatccgcaga cgatcgcgcg cgatccgttg ctgccgcgcg tgtgcgcgac gctcgtcgag cgcgcactgc accatttcgc gcaggccgac gcggtgatgg acgcgtcgcc gcgcgcgcag gttcgtgcgc cgcgcatcat gtcgggcgcg tatcgcctga ttctcgaagc cgcggtcgcg cgcggcttcg cgccgccgcg cgcgccgctg cgcaagccgc gcgcgcgaat gctgctgctc gcggcgcgtt acgcgctgtt ttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDDNETDA AAVTSGSSFY LAMRILPAEQ RDAMFQVYAF CRAVDDIADE GGARAERAAQ LDRWRADIDD CYAGKPRASL VPLAREIGKF GLHRDDFHAM IDGMAMDAAE DICAPDEATL DLYCDRVASS AGRLSVRIFG MPDEPGRVLS HHLGRALQLT NILRDIDDDA AINRCYLPRE LLAREGIAIA DPQTIARDPL LPRVCATLVE RALHHFAQAD AVMDASPRAQ VRAPRIMSGA YRLILEAAVA RGFAPPRAPL RKPRARMLLL AARYALF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggacgat aacgaaaccg acgccgctgc cgtcacatcg ggcagctctt tctatctcgc gatgcgcatc ctgcccgcgg agcagcgcga tgcgatgttc caggtctatg cgttttgccg tgcggtcgac gacatcgccg acgaaggcgg cgcgcgcgcg gagcgcgcgg cgcagctcga ccgctggcgc gccgacatcg atgattgcta cgcgggcaag ccgcgcgcgt cgctcgtgcc gctcgcgcgc gagatcggca agttcggcct gcatcgcgac gactttcacg cgatgatcga cggcatggcg atggacgccg ccgaggacat ctgcgcgccc gacgaagcca cgctcgatct gtactgcgac cgcgtcgcga gctcggccgg ccggctatcg gtgaggattt tcgggatgcc ggacgagccc ggccgcgtac tgtcgcatca tctcggccgc gcgctgcaac tgacgaatat cctgcgcgac atcgacgatg acgcggcgat caaccgttgc tacctgccgc gcgagctgct cgcgcgcgag ggcatcgcga tcgccgatcc gcagacgatc gcgcgcgatc cgttgctgcc gcgcgtgtgc gcgacgctcg tcgagcgcgc actgcaccat ttcgcgcagg ccgacgcggt gatggacgcg tcgccgcgcg cgcaggttcg tgcgccgcgc atcatgtcgg gcgcgtatcg cctgattctc gaagccgcgg tcgcgcgcgg cttcgcgccg ccgcgcgcgc cgctgcgcaa gccgcgcgcg cgaatgctgc tgctcgcggc gcgttacgcg ctgtttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsA.12707.a.A1.PW27545
PURIFICATION DATe: 11/9/2009
CONCENTRATION: 27.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDDNETDA AAVTSGSSFY LAMRILPAEQ RDAMFQVYAF CRAVDDIADE GGARAERAAQ LDRWRADIDD CYAGKPRASL VPLAREIGKF GLHRDDFHAM IDGMAMDAAE DICAPDEATL DLYCDRVASS AGRLSVRIFG MPDEPGRVLS HHLGRALQLT NILRDIDDDA AINRCYLPRE LLAREGIAIA DPQTIARDPL LPRVCATLVE RALHHFAQAD AVMDASPRAQ VRAPRIMSGA YRLILEAAVA RGFAPPRAPL RKPRARMLLL AARYALF
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg acgataacga aaccgacgcc gctgccgtca catcgggcag ctctttctat ctcgcgatgc gcatcctgcc cgcggagcag cgcgatgcga tgttccaggt ctatgcgttt tgccgtgcgg tcgacgacat cgccgacgaa ggcggcgcgc gcgcggagcg cgcggcgcag ctcgaccgct ggcgcgccga catcgatgat tgctacgcgg gcaagccgcg cgcgtcgctc gtgccgctcg cgcgcgagat cggcaagttc ggcctgcatc gcgacgactt tcacgcgatg atcgacggca tggcgatgga cgccgccgag gacatctgcg cgcccgacga agccacgctc gatctgtact gcgaccgcgt cgcgagctcg gccggccggc tatcggtgag gattttcggg atgccggacg agcccggccg cgtactgtcg catcatctcg gccgcgcgct gcaactgacg aatatcctgc gcgacatcga cgatgacgcg gcgatcaacc gttgctacct gccgcgcgag ctgctcgcgc gcgagggcat cgcgatcgcc gatccgcaga cgatcgcgcg cgatccgttg ctgccgcgcg tgtgcgcgac gctcgtcgag cgcgcactgc accatttcgc gcaggccgac gcggtgatgg acgcgtcgcc gcgcgcgcag gttcgtgcgc cgcgcatcat gtcgggcgcg tatcgcctga ttctcgaagc cgcggtcgcg cgcggcttcg cgccgccgcg cgcgccgctg cgcaagccgc gcgcgcgaat gctgctgctc gcggcgcgtt acgcgctgtt ttaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDDNETDA AAVTSGSSFY LAMRILPAEQ RDAMFQVYAF CRAVDDIADE GGARAERAAQ LDRWRADIDD CYAGKPRASL VPLAREIGKF GLHRDDFHAM IDGMAMDAAE DICAPDEATL DLYCDRVASS AGRLSVRIFG MPDEPGRVLS HHLGRALQLT NILRDIDDDA AINRCYLPRE LLAREGIAIA DPQTIARDPL LPRVCATLVE RALHHFAQAD AVMDASPRAQ VRAPRIMSGA YRLILEAAVA RGFAPPRAPL RKPRARMLLL AARYALF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGACGAT AACGAAACCG ACGCCGCTGC CGTCACATCG GGCAGCTCTT TCTATCTCGC GATGCGCATC CTGCCCGCGG AGCAGCGCGA TGCGATGTTC CAGGTCTATG CGTTTTGCCG TGCGGTCGAC GACATCGCCG ACGAAGGCGG CGCGCGCGCG GAGCGCGCGG CGCAGCTCGA CCGCTGGCGC GCCGACATCG ATGATTGCTA CGCGGGCAAG CCGCGCGCGT CGCTCGTGCC GCTCGCGCGC GAGATCGGCA AGTTCGGCCT GCATCGCGAC GACTTTCACG CGATGATCGA CGGCATGGCG ATGGACGCCG CCGAGGACAT CTGCGCGCCC GACGAAGCCA CGCTCGATCT GTACTGCGAC CGCGTCGCGA GCTCGGCCGG CCGGCTATCG GTGAGGATTT TCGGGATGCC GGACGAGCCC GGCCGCGTAC TGTCGCATCA TCTCGGCCGC GCGCTGCAAC TGACGAATAT CCTGCGCGAC ATCGACGATG ACGCGGCGAT CAACCGTTGC TACCTGCCGC GCGAGCTGCT CGCGCGCGAG GGCATCGCGA TCGCCGATCC GCAGACGATC GCGCGCGATC CGTTGCTGCC GCGCGTGTGC GCGACGCTCG TCGAGCGCGC ACTGCACCAT TTCGCGCAGG CCGACGCGGT GATGGACGCG TCGCCGCGCG CGCAGGTTCG TGCGCCGCGC ATCATGTCGG GCGCGTATCG CCTGATTCTC GAAGCCGCGG TCGCGCGCGG CTTCGCGCCG CCGCGCGCGC CGCTGCGCAA GCCGCGCGCG CGAATGCTGC TGCTCGCGGC GCGTTACGCG CTGTTTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA