BupsA.12245.b

allantoate amidohydrolase

CENTER ID: BupsA.12245.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.12245.b.A1.GE27681 Full length( BupsA.12245.b ) 1 459
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.4235
RefSeq: YP_335411.1
UniProt: Q3JLZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MWEIDGRRLW NSLTELARIG GTARGGVRRL ALTEADRRGR ERFAQWCRDA GMAVRVDAIG NLFARRAGAD DTQPAVLVGS HLDTQPEGGR FDGAYGVLAG LELVRTLNDR GIVTGKPIEI VSWTNEEGVR FTPAMLGSAV FTGALPLDAA LAARDADGIA LAEALEACGY RDALRSAWMR GAHGARGLEP ALAPLHERVD AYFEAHIEQG PVLERHGATI GVVTGGQAIR WLDATVTGAA AHAGTTPMPY RKDALFASAE LALAIEALVA RHAPDALATI GQSAIDNASR NTIAERVTFS IDLRHPDDAR LDAIERELRR VCAQIAGRRG VAVDISQHWS SPATPFDPAC IALVEAAARG GGYAHERIVS GAGHDAVHLA RCVPSAMVFI PCVGGLSHNA AERALPEHVE AGANVLLGAV LARAGVRERA ARSARSPRPA PAMQAAHSGT DAGTFAQPH
NT Sequence
GTGTGGGAAA TTGACGGTAG GCGGCTCTGG AACAGCCTGA CGGAACTCGC GCGAATCGGC GGCACCGCGC GCGGCGGCGT GCGCCGGCTC GCGCTGACCG AGGCCGACCG GCGCGGCCGC GAACGCTTCG CGCAATGGTG TCGCGACGCG GGGATGGCGG TGCGCGTCGA TGCGATCGGC AACCTGTTCG CCCGGCGCGC CGGCGCGGAC GACACGCAGC CGGCCGTGCT CGTCGGCAGT CATCTCGACA CGCAGCCCGA AGGGGGGCGC TTCGACGGCG CGTACGGCGT GCTCGCCGGG CTCGAGCTCG TGCGCACGCT GAACGATCGC GGGATCGTCA CCGGCAAGCC GATCGAGATC GTATCGTGGA CCAACGAGGA GGGCGTGCGC TTCACGCCGG CGATGCTCGG CTCGGCGGTG TTTACCGGCG CATTGCCGCT CGACGCGGCG CTCGCCGCGC GCGATGCGGA CGGCATCGCG CTTGCCGAAG CGCTCGAAGC GTGCGGCTAT CGCGATGCGC TGCGCTCGGC GTGGATGCGC GGCGCGCACG GGGCGCGCGG CCTGGAGCCG GCGCTCGCGC CGCTGCACGA GCGCGTCGAT GCGTACTTCG AGGCGCATAT CGAGCAGGGC CCGGTGCTGG AGCGGCACGG CGCGACGATC GGCGTCGTCA CGGGCGGGCA GGCGATCCGC TGGCTCGACG CGACGGTCAC GGGTGCGGCC GCGCATGCCG GCACGACGCC GATGCCGTAC CGCAAGGACG CGCTGTTCGC GAGCGCCGAG CTCGCGCTCG CGATCGAGGC GCTCGTCGCG CGCCATGCGC CCGACGCGCT CGCGACGATC GGGCAGAGCG CGATCGACAA CGCGTCGCGC AACACGATCG CCGAGCGCGT CACGTTCAGC ATCGATCTTC GCCATCCCGA CGATGCGCGG CTCGACGCGA TCGAGCGCGA GCTGCGCCGC GTGTGCGCGC AAATCGCCGG GCGACGCGGC GTCGCGGTCG ACATCAGCCA GCACTGGTCG AGCCCGGCGA CGCCGTTCGA TCCGGCGTGC ATCGCGCTCG TCGAGGCGGC CGCGCGCGGC GGCGGCTATG CGCACGAGCG GATCGTGAGC GGCGCCGGGC ACGATGCGGT TCATCTCGCG CGCTGCGTGC CGAGCGCGAT GGTGTTCATT CCGTGCGTCG GCGGGCTGTC GCACAACGCA GCCGAGCGTG CGTTGCCCGA GCACGTCGAG GCGGGCGCGA ACGTGCTGCT CGGCGCGGTG CTCGCGCGCG CCGGCGTGCG CGAGCGGGCG GCGCGATCGG CGCGCTCGCC GCGCCCGGCG CCGGCGATGC AGGCCGCGCA TTCGGGCACG GACGCGGGCA CCTTCGCGCA GCCGCAT
Details for BupsA.12245.b.A1.GE27681
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 71
PERCENT COVERAGE: 81
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVWEIDGRR LWNSLTELAR IGGTARGGVR RLALTEADRR GRERFAQWCR DAGMAVRVDA IGNLFARRAG ADDTQPAVLV GSHLDTQPEG GRFDGAYGVL AGLELVRTLN DRGIVTGKPI EIVSWTNEEG VRFTPAMLGS AVFTGALPLD AALAARDADG IALAEALEAC GYRDALRSAW MRGAHGARGL EPALAPLXXR VXAYFXAHIE QGPVLXRXGA TIGVXXGGXX IRXXDXXVTG AAAHAXRXRX XXAXXRXXXA PXSRSXSRRS SRAMRXXXXX XSGRAXXXTR RATRSPSASR SAXIFAIPTM RGSTRSSASC AACARKSPGD AASRSTSAST GRARRRRSIR RASRSSRRPR AAAAMRTSGS
Validated NT Sequence
taatgcggct gcgcgaaggt gcccgcgtcc gtgcccgaat gcgcggcctg catcgccggc gccgggcgcg gcgagcgcgc cgatcgcgcc gcccgctcgc gcacgccggc gcgcgcgagc accgcgccga gcagcacgtt cgcgcccgcc tcgacgtgct cgggcaacgc acgctcggct gcgttgtgcg acagcccgcc gacgcacgga atgaacacca tcgcgctcgg cacgcagcgc gcgagatgaa ccgcatcgtg cccggcgccg ctcacgatcc gctcgtgcgc atagccgccg ccgcgcgcgg ccgcctcgac gagcgcgatg cacgccggat cgaacggcgt cgccgggctc gaccagtgct ggctgatgtc gaccgcgacg ccgcgtcgcc cggcgatttg cgcgcacacg cggcgcagct cgcgctcgat cgcgtcgagc cgcgcatcgt cgggatggcg aagatcnatg ctgaacgtga cgcgctcggc gatcgtgttg cgcgacgcgt tgncnanngc gctctgcccg atnnnnncgn gnnnnnnngg cgcatggcgc gcgacgagcg cctcgatngc gagcgcgagc ncggcgctcn cgnncagcgc nncnntgcgn tangncatcg ncntcgtncg gcatgcgcgg ccgcacccgt gaccgnngng tcganncagc ggatcgnnnn nccgcccgng angacgccga tngtcgcgcc gngncgntcc agcaccgggc cctgctcgat atgcgcctng aagtacgcat ngacgcgntc gngcagnggc gcgagcgccg gctccaggcc gcgcgccccg tgcgcgccgc gcatccacgc cgagcgcagc gcatcgcgat agccgcacgc ttcgagcgct tcggcaagcg cgatgccgtc cgcatcgcgc gcggcgagcg ccgcgtcgag cggcaatgcg ccggtaaaca ccgccgagcc gagcatcgcc ggcgtgaagc gcacgccctc ctcgttggtc cacgatacga tctcgatcgg cttgccggtg acgatcccgc gatcgttcag cgtgcgcacg agctcgagcc cggcgagcac gccgtacgcg ccgtcgaagc gccccccttc gggctgcgtg tcgagatgac tgccgacgag cacggccggc tgcgtgtcgt ccgcgccggc gcgccgggcg aacaggttgc cgatcgcatc gacgcgcacc gccatccccg cgtcgcgaca ccattgcgcg aagcgttcgc ggccgcgccg gtcggcctcg gtcagcgcga gccggcgcac gccgccgcgc gcggtgccgc cgattcgcgc gagttccgtc aggctgttcc agagccgcct accgtcaatt tcccacacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVWEIDGRR LWNSLTELAR IGGTARGGVR RLALTEADRR GRERFAQWCR DAGMAVRVDA IGNLFARRAG ADDTQPAVLV GSHLDTQPEG GRFDGAYGVL AGLELVRTLN DRGIVTGKPI EIVSWTNEEG VRFTPAMLGS AVFTGALPLD AALAARDADG IALAEALEAC GYRDALRSAW MRGAHGARGL EPALAPLHER VDAYFEAHIE QGPVLERHGA TIGVVTGGQA IRWLDATVTG AAAHAGTTPM PYRKDALFAS AELALAIEAL VARHAPDALA TIGQSAIDNA SRNTIAERVT FSIDLRHPDD ARLDAIEREL RRVCAQIAGR RGVAVDISQH WSSPATPFDP ACIALVEAAA RGGGYAHERI VSGAGHDAVH LARCVPSAMV FIPCVGGLSH NAAERALPEH VEAGANVLLG AVLARAGVRE RAARSARSPR PAPAMQAAHS GTDAGTFAQP H
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtgggaa attgacggta ggcggctctg gaacagcctg acggaactcg cgcgaatcgg cggcaccgcg cgcggcggcg tgcgccggct cgcgctgacc gaggccgacc ggcgcggccg cgaacgcttc gcgcaatggt gtcgcgacgc ggggatggcg gtgcgcgtcg atgcgatcgg caacctgttc gcccggcgcg ccggcgcgga cgacacgcag ccggccgtgc tcgtcggcag tcatctcgac acgcagcccg aaggggggcg cttcgacggc gcgtacggcg tgctcgccgg gctcgagctc gtgcgcacgc tgaacgatcg cgggatcgtc accggcaagc cgatcgagat cgtatcgtgg accaacgagg agggcgtgcg cttcacgccg gcgatgctcg gctcggcggt gtttaccggc gcattgccgc tcgacgcggc gctcgccgcg cgcgatgcgg acggcatcgc gcttgccgaa gcgctcgaag cgtgcggcta tcgcgatgcg ctgcgctcgg cgtggatgcg cggcgcgcac ggggcgcgcg gcctggagcc ggcgctcgcg ccgctgcacg agcgcgtcga tgcgtacttc gaggcgcata tcgagcaggg cccggtgctg gagcggcacg gcgcgacgat cggcgtcgtc acgggcgggc aggcgatccg ctggctcgac gcgacggtca cgggtgcggc cgcgcatgcc ggcacgacgc cgatgccgta ccgcaaggac gcgctgttcg cgagcgccga gctcgcgctc gcgatcgagg cgctcgtcgc gcgccatgcg cccgacgcgc tcgcgacgat cgggcagagc gcgatcgaca acgcgtcgcg caacacgatc gccgagcgcg tcacgttcag catcgatctt cgccatcccg acgatgcgcg gctcgacgcg atcgagcgcg agctgcgccg cgtgtgcgcg caaatcgccg ggcgacgcgg cgtcgcggtc gacatcagcc agcactggtc gagcccggcg acgccgttcg atccggcgtg catcgcgctc gtcgaggcgg ccgcgcgcgg cggcggctat gcgcacgagc ggatcgtgag cggcgccggg cacgatgcgg ttcatctcgc gcgctgcgtg ccgagcgcga tggtgttcat tccgtgcgtc ggcgggctgt cgcacaacgc agccgagcgt gcgttgcccg agcacgtcga ggcgggcgcg aacgtgctgc tcggcgcggt gctcgcgcgc gccggcgtgc gcgagcgggc ggcgcgatcg gcgcgctcgc cgcgcccggc gccggcgatg caggccgcgc attcgggcac ggacgcgggc accttcgcgc agccgcataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa