BupsA.10825.a

cell division protein FtsQ

CENTER ID: BupsA.10825.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10825.a.A2.GE27677 Mature protein( BupsA.10825.a ) 26 250
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3686
RefSeq: YP_334911.1
UniProt: Q3JNE2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MWNNVRQLNL AASALYALLL LVLAAAGCYW LIQRPAFALR EIRIDGDTEH INAPTVRAGV VGRLKGNFFT VDLDLARVAF EQMPWVRHAS VRRVWPNALA VTLEEYKPLG TWGNDQLVSV DGELFTANQG ELDAELPSFD GPEGSAKEVV ARYRDFAKWF APIHATPEEV TLSPRYAWTV KLSNGMQVEL GRERNSDTLP DRIQRLVAAW PSVTQRWGGD IEYADLRYPN GFAIRAAGMR FLTDTDKGKK
NT Sequence
ATGTGGAATA ACGTTCGCCA ACTCAACCTT GCCGCCAGCG CGTTGTACGC GCTGCTGCTG CTCGTGTTGG CGGCGGCCGG CTGCTACTGG CTGATCCAGC GCCCGGCGTT CGCGCTGCGG GAAATCCGCA TCGACGGGGA CACCGAGCAC ATCAACGCGC CGACCGTGCG CGCGGGCGTC GTCGGGCGGC TGAAGGGCAA CTTCTTCACC GTCGATCTCG ACCTCGCGCG CGTCGCGTTC GAGCAGATGC CGTGGGTGCG CCACGCGAGC GTGCGCCGGG TGTGGCCGAA CGCGCTCGCC GTGACGCTCG AAGAGTACAA GCCGCTCGGC ACGTGGGGCA ACGATCAGCT CGTGAGCGTC GACGGCGAGC TCTTCACCGC GAACCAGGGC GAGCTCGATG CGGAGCTGCC GTCGTTCGAC GGCCCGGAGG GCAGCGCGAA GGAGGTCGTC GCGCGCTATC GCGACTTCGC GAAATGGTTT GCGCCGATCC ACGCGACGCC TGAGGAGGTG ACGCTGTCGC CGCGCTACGC GTGGACGGTG AAGCTGTCGA ACGGCATGCA GGTCGAGCTC GGCCGCGAGC GCAACAGCGA CACGCTGCCC GACCGGATCC AGCGCCTCGT CGCCGCATGG CCGTCGGTCA CGCAGCGCTG GGGCGGCGAC ATCGAGTACG CGGATCTTCG CTATCCGAAC GGATTCGCGA TTCGCGCGGC GGGCATGCGG TTCCTGACCG ATACCGACAA GGGCAAGAAG
Details for BupsA.10825.a.A2.GE27677
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 27kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAGCYWLIQ RPAFALREIR IDGDTEHINA PTVRAGVVGR LKGNFFTVDL DLARVAFEQM PWVRHASVRR VWPNALAVTL EEYKPLGTWG NDQLVSVDGE LFTANQGELD AELPSFDGPE GSAKEVVARY RDFAKWFAPI HATPEEVTLS PRYAWTVKLS NGMQVELGRE RNSDTLPDRI QRLVAAWPSV TQRWGGDIEY ADLRYPNGFA IRAAGMRFLT DTDKG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg gctgctactg gctgatccag cgcccggcgt tcgcgctgcg ggaaatccgc atcgacgggg acaccgagca catcaacgcg ccgaccgtgc gcgcgggcgt cgtcgggcgg ctgaagggca acttcttcac cgtcgatctc gacctcgcgc gcgtcgcgtt cgagcagatg ccgtgggtgc gccacgcgag cgtgcgccgg gtgtggccga acgcgctcgc cgtgacgctc gaagagtaca agccgctcgg cacgtggggc aacgatcagc tcgtgagcgt cgacggcgag ctcttcaccg cgaaccaggg cgagctcgat gcggagctgc cgtcgttcga cggcccggag ggcagcgcga aggaggtcgt cgcgcgctat cgcgacttcg cgaaatggtt tgcgccgatc cacgcgacgc ctgaggaggt gacgctgtcg ccgcgctacg cgtggacggt gaagctgtcg aacggcatgc aggtcgagct cggccgcgag cgcaacagcg acacgctgcc cgaccggatc cagcgcctcg tcgccgcatg gccgtcggtc acgcagcgct ggggcggcga catcgagtac gcggatcttc gctatccgaa cggattcgcg attcgcgcgg cgggcatgcg gttcctgacc gataccgaca agggca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGCYWLIQ RPAFALREIR IDGDTEHINA PTVRAGVVGR LKGNFFTVDL DLARVAFEQM PWVRHASVRR VWPNALAVTL EEYKPLGTWG NDQLVSVDGE LFTANQGELD AELPSFDGPE GSAKEVVARY RDFAKWFAPI HATPEEVTLS PRYAWTVKLS NGMQVELGRE RNSDTLPDRI QRLVAAWPSV TQRWGGDIEY ADLRYPNGFA IRAAGMRFLT DTDKGKK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccggctgc tactggctga tccagcgccc ggcgttcgcg ctgcgggaaa tccgcatcga cggggacacc gagcacatca acgcgccgac cgtgcgcgcg ggcgtcgtcg ggcggctgaa gggcaacttc ttcaccgtcg atctcgacct cgcgcgcgtc gcgttcgagc agatgccgtg ggtgcgccac gcgagcgtgc gccgggtgtg gccgaacgcg ctcgccgtga cgctcgaaga gtacaagccg ctcggcacgt ggggcaacga tcagctcgtg agcgtcgacg gcgagctctt caccgcgaac cagggcgagc tcgatgcgga gctgccgtcg ttcgacggcc cggagggcag cgcgaaggag gtcgtcgcgc gctatcgcga cttcgcgaaa tggtttgcgc cgatccacgc gacgcctgag gaggtgacgc tgtcgccgcg ctacgcgtgg acggtgaagc tgtcgaacgg catgcaggtc gagctcggcc gcgagcgcaa cagcgacacg ctgcccgacc ggatccagcg cctcgtcgcc gcatggccgt cggtcacgca gcgctggggc ggcgacatcg agtacgcgga tcttcgctat ccgaacggat tcgcgattcg cgcggcgggc atgcggttcc tgaccgatac cgacaagggc aagaagaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa