BupsA.10647.a

penicillin-binding protein

CENTER ID: BupsA.10647.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10647.a.A2.GE27676 Mature protein( BupsA.10647.a ) 49 594
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.4179
RefSeq: YP_335372.1
UniProt: Q3JM31

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLNKKPKSS DPYVAVAKNP MLSSSLPMWR SKFVVILVFL AFGALIARAF WVQVANQDFY VGQGQKRYQR TIELGAMRGS IVDRNGALLA VSLTTYEIWA NPKQVDEAAH APLAKLLDMP LAEVRRRLTG DRSFVLLKRQ VDPETAARIE KLDIDGVTQI ADSKRFYPEG ESAAHVVGFT NVEDRGQEGV ELAANGRLSG TAGQRQVIRD RLGRIVSDTG PLVPAQHGAT IELTIDRRIQ QLAYAQLKST IIANNAQAGS VVVLDAQNGE ILALANYPTF DPNDRARLTG QQLRNRAVID TFEPGSTIKP VVVALSIDAG KVRPQTIIDT SPGAYKIGPN VIHDTSNHGA ITVAQALQMS SNIALAKLAL NLPAETIWNK YQEYGIGRAP ELTFPGVASG RLRPYKRWRP IEQATMAYGY GLSTSLLQIA QVYTAYAGDG TIHPVSLLKA SADPQSADAH RGHRVTTPAT AAAIRSMLEM AVGQGGTGRA ARVDGYRVGG KTGTARKQVG ASYVKGKYRA LFAGMAPMSN PRLIIAVMID EPAARSYYGG TVAGPVFSSV MSGSLQLLGV PPDAPIAADK PAGAGAKPQA KAKT
NT Sequence
ATGCGCCTGA ACAAAAAACC GAAGTCATCC GATCCTTACG TCGCCGTCGC GAAAAATCCG ATGCTGTCGT CGAGCCTGCC GATGTGGCGC TCGAAATTCG TCGTGATCCT CGTGTTCCTC GCGTTCGGCG CGCTGATCGC CCGCGCGTTC TGGGTGCAGG TGGCGAATCA GGATTTCTAC GTCGGCCAGG GCCAGAAGCG CTATCAGCGC ACCATCGAGC TCGGCGCGAT GCGCGGCAGC ATCGTCGATC GCAACGGCGC GCTGCTCGCC GTGAGCCTCA CCACCTACGA AATCTGGGCG AACCCGAAGC AGGTCGACGA AGCCGCGCAC GCGCCGCTCG CGAAGCTGCT CGACATGCCG CTCGCCGAAG TGCGGCGGCG CCTGACGGGC GATCGCTCGT TCGTGCTGCT CAAGCGCCAG GTCGATCCCG AAACGGCCGC GCGCATCGAA AAACTCGACA TCGACGGCGT CACGCAGATC GCCGATTCGA AGCGCTTCTA TCCGGAAGGC GAATCGGCCG CGCACGTCGT CGGCTTCACG AACGTCGAGG ATCGCGGGCA GGAAGGCGTC GAGCTCGCCG CGAACGGGCG GCTGTCCGGC ACCGCGGGCC AACGCCAGGT GATCCGCGAC CGGCTCGGGC GGATCGTCTC CGATACCGGC CCGCTCGTGC CCGCGCAGCA CGGTGCGACG ATCGAGCTGA CGATCGACCG CCGGATCCAG CAGCTCGCGT ACGCGCAGCT GAAGTCGACG ATCATCGCGA ACAACGCGCA GGCAGGCAGC GTCGTCGTGC TGGACGCGCA GAACGGCGAA ATCCTCGCGC TCGCGAACTA TCCGACATTC GATCCGAACG ATCGCGCGCG GCTGACGGGC CAGCAATTGC GCAACCGCGC GGTGATCGAC ACGTTCGAGC CGGGCTCGAC GATCAAGCCG GTCGTCGTCG CGCTATCGAT CGACGCCGGC AAGGTGCGGC CACAGACGAT CATCGACACG TCGCCGGGCG CGTACAAGAT CGGCCCGAAC GTGATTCACG ACACGTCGAA CCACGGCGCG ATCACCGTCG CGCAGGCGCT GCAGATGTCG AGCAACATCG CGCTCGCGAA GCTCGCGCTG AACCTGCCTG CCGAGACGAT CTGGAACAAG TACCAGGAAT ACGGCATCGG CCGCGCGCCG GAGCTGACAT TCCCCGGCGT CGCGTCCGGG CGGCTGCGCC CGTACAAGCG CTGGCGGCCG ATCGAGCAGG CGACGATGGC GTACGGCTAC GGGCTGTCGA CATCGCTGCT GCAGATCGCG CAGGTCTACA CCGCGTATGC GGGCGACGGC ACGATCCATC CGGTGTCGCT ACTCAAGGCG AGCGCCGATC CGCAGTCGGC CGACGCGCAC CGCGGCCACC GCGTGACGAC GCCCGCCACC GCGGCCGCGA TCCGCTCGAT GCTCGAGATG GCCGTCGGCC AAGGCGGCAC CGGGCGCGCA GCGCGCGTCG ACGGTTACCG CGTCGGCGGC AAGACGGGGA CCGCGCGCAA GCAAGTCGGC GCGTCGTACG TCAAGGGCAA GTATCGCGCG CTGTTCGCCG GGATGGCGCC GATGAGCAAT CCGCGCCTCA TCATCGCGGT GATGATCGAC GAGCCGGCCG CGCGCAGCTA CTACGGCGGC ACGGTCGCGG GGCCGGTGTT CTCGTCGGTG ATGAGCGGCT CCCTGCAACT GCTCGGCGTG CCGCCCGACG CACCCATCGC GGCGGACAAG CCGGCCGGCG CCGGCGCGAA GCCGCAGGCG AAGGCAAAAA CG
Details for BupsA.10647.a.A2.GE27676
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 60kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 63
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAFWVQVAN QDFYVGQGQK RYQRTIELGA MRGSIVDRNG ALLAVSLTTY EIWANPKQVD EAAHAPLAKL LDMPLAEVRR RLTGDRSFVL LKRQVDPETA ARIEKLDIDG VTQIADSKRF YPEGESAAHV VGFTNVEDRG QEGVELAANG RLSGTAGQRQ VIRDRLGRIV SDTGPLVPAQ HGATIELTID RRIQQLAYAQ LKSTIIANNA XAGSVVVLDA QNGEILALAN YPTFDPNDRA RLTGQQLRNR AVIDTFEPGS TIKPVVVALS IDAXXXRPQT IIDXXXGRVX DXPERDXXHV EPRRDHRRAG AADVEXHRAR EARAEPACRD DXEQVPGIRH RPRAGADIPX RRVRAAAPVQ ALAADRAGDD GVRLRAVDIA AADRAGLHRV CGRRHDPSGV ATQGERRSAV GRRAPRPPRD DARHRGRDPL DARDGRRPRR HRARSARRRL PRRRQDGDRA QASRRVVRQG QVSRAVRRDG ADEQSAPHHR GDDRRAGRAQ LLRRHGRGAG VLVGDERLPA TARRAARRTH RGGQAGRRRR EAAGEG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgt tctgggtgca ggtggcgaat caggatttct acgtcggcca gggccagaag cgctatcagc gcaccatcga gctcggcgcg atgcgcggca gcatcgtcga tcgcaacggc gcgctgctcg ccgtgagcct caccacctac gaaatctggg cgaacccgaa gcaggtcgac gaagccgcgc acgcgccgct cgcgaagctg ctcgacatgc cgctcgccga agtgcggcgg cgcctgacgg gcgatcgctc gttcgtgctg ctcaagcgcc aggtcgatcc cgaaacggcc gcgcgcatcg aaaaactcga catcgacggc gtcacgcaga tcgccgattc gaagcgcttc tatccggaag gcgaatcggc cgcgcacgtc gtcggcttca cgaacgtcga ggatcgcggg caggaaggcg tcgagctcgc cgcgaacggg cggctgtccg gcaccgcggg ccaacgccag gtgatccgcg accggctcgg gcggatcgtc tccgataccg gcccgctcgt gcccgcgcag cacggtgcga cgatcgagct gacgatcgac cgccggatcc agcagctcgc gtacgcgcag ctgaagtcga cgatcatcgc gaacaacgcg cangcaggca gcgtcgtcgt gctggacgcg cagaacggcg aaatcctcgc gctcgcgaac tatccgacat tcgatccgaa cgatcgcgcg cggctgacgg gccagcaatt gcgcaaccgc gcggtgatcg acacgttcga gccgggntcg acgatcaagc cggtcgtcgt cgcgctatcg atcgacgccn gnanggngcg gccacagacg atcatcgacn cntnnncngg gcgcgtacnn gatcnncccg aacgtgatnc nngncacgtc gaaccncggc gcgatcaccg tcgcgcnggc gctgcagatg tcgagcanca tcgcgctcgc gaagctcgcg ctgaacctgc ctgccgagac gatntggaac aagtaccagg aatacggcat cggccgcgcg ccggagctga cattcccngg cgtcgcgtcc gggcggctgc gcccgtacaa gcgctggcgg ccgatcgagc aggcgacgat ggcgtacggc tacgggctgt cgacatcgct gctgcagatc gcgcaggtct acaccgcgta tgcgggcgac ggcacgatcc atccggtgtc gctactcaag gcgagcgccg atccgcagtc ggccgacgcg caccgcggcc accgcgtgac gacgcccgcc accgcggccg cgatccgctc gatgctcgag atggccgtcg gccaaggcgg caccgggcgc gcagcgcgcg tcgacggtta ccgcgtcggc ggcaagacgg ggaccgcgcg caagcaagtc ggcgcgtcgt acgtcaaggg caagtatcgc gcgctgttcg ccgggatggc gccgatgagc aatccgcgcc tcatcatcgc ggtgatgatc gacgagccgg ccgcgcgcag ctactacggc ggcacggtcg cggggccggt gttctcgtcg gtgatgagcg gctccctgca actgctcggc gtgccgcccg acgcacccat cgcggcggac aagccggccg gcgccggcgc gaagccgcag gcgaaggcaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFWVQVAN QDFYVGQGQK RYQRTIELGA MRGSIVDRNG ALLAVSLTTY EIWANPKQVD EAAHAPLAKL LDMPLAEVRR RLTGDRSFVL LKRQVDPETA ARIEKLDIDG VTQIADSKRF YPEGESAAHV VGFTNVEDRG QEGVELAANG RLSGTAGQRQ VIRDRLGRIV SDTGPLVPAQ HGATIELTID RRIQQLAYAQ LKSTIIANNA QAGSVVVLDA QNGEILALAN YPTFDPNDRA RLTGQQLRNR AVIDTFEPGS TIKPVVVALS IDAGKVRPQT IIDTSPGAYK IGPNVIHDTS NHGAITVAQA LQMSSNIALA KLALNLPAET IWNKYQEYGI GRAPELTFPG VASGRLRPYK RWRPIEQATM AYGYGLSTSL LQIAQVYTAY AGDGTIHPVS LLKASADPQS ADAHRGHRVT TPATAAAIRS MLEMAVGQGG TGRAARVDGY RVGGKTGTAR KQVGASYVKG KYRALFAGMA PMSNPRLIIA VMIDEPAARS YYGGTVAGPV FSSVMSGSLQ LLGVPPDAPI AADKPAGAGA KPQAKAKT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgttctgg gtgcaggtgg cgaatcagga tttctacgtc ggccagggcc agaagcgcta tcagcgcacc atcgagctcg gcgcgatgcg cggcagcatc gtcgatcgca acggcgcgct gctcgccgtg agcctcacca cctacgaaat ctgggcgaac ccgaagcagg tcgacgaagc cgcgcacgcg ccgctcgcga agctgctcga catgccgctc gccgaagtgc ggcggcgcct gacgggcgat cgctcgttcg tgctgctcaa gcgccaggtc gatcccgaaa cggccgcgcg catcgaaaaa ctcgacatcg acggcgtcac gcagatcgcc gattcgaagc gcttctatcc ggaaggcgaa tcggccgcgc acgtcgtcgg cttcacgaac gtcgaggatc gcgggcagga aggcgtcgag ctcgccgcga acgggcggct gtccggcacc gcgggccaac gccaggtgat ccgcgaccgg ctcgggcgga tcgtctccga taccggcccg ctcgtgcccg cgcagcacgg tgcgacgatc gagctgacga tcgaccgccg gatccagcag ctcgcgtacg cgcagctgaa gtcgacgatc atcgcgaaca acgcgcaggc aggcagcgtc gtcgtgctgg acgcgcagaa cggcgaaatc ctcgcgctcg cgaactatcc gacattcgat ccgaacgatc gcgcgcggct gacgggccag caattgcgca accgcgcggt gatcgacacg ttcgagccgg gctcgacgat caagccggtc gtcgtcgcgc tatcgatcga cgccggcaag gtgcggccac agacgatcat cgacacgtcg ccgggcgcgt acaagatcgg cccgaacgtg attcacgaca cgtcgaacca cggcgcgatc accgtcgcgc aggcgctgca gatgtcgagc aacatcgcgc tcgcgaagct cgcgctgaac ctgcctgccg agacgatctg gaacaagtac caggaatacg gcatcggccg cgcgccggag ctgacattcc ccggcgtcgc gtccgggcgg ctgcgcccgt acaagcgctg gcggccgatc gagcaggcga cgatggcgta cggctacggg ctgtcgacat cgctgctgca gatcgcgcag gtctacaccg cgtatgcggg cgacggcacg atccatccgg tgtcgctact caaggcgagc gccgatccgc agtcggccga cgcgcaccgc ggccaccgcg tgacgacgcc cgccaccgcg gccgcgatcc gctcgatgct cgagatggcc gtcggccaag gcggcaccgg gcgcgcagcg cgcgtcgacg gttaccgcgt cggcggcaag acggggaccg cgcgcaagca agtcggcgcg tcgtacgtca agggcaagta tcgcgcgctg ttcgccggga tggcgccgat gagcaatccg cgcctcatca tcgcggtgat gatcgacgag ccggccgcgc gcagctacta cggcggcacg gtcgcggggc cggtgttctc gtcggtgatg agcggctccc tgcaactgct cggcgtgccg cccgacgcac ccatcgcggc ggacaagccg gccggcgccg gcgcgaagcc gcaggcgaag gcaaaaacga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a