SSGCID: Target Page

BupsA.10611.a

glutathione-dependent formaldehyde dehydrogenase

CENTER ID:	BupsA.10611.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.10611.a.A1.GE27675	Full length( BupsA.10611.a )		1	368

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.1035
RefSeq:	YP_332437.1
UniProt:	Q3JVG6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKTKAAIAWK AGEPLTIEEV DLEGPRAGEV LIEVKATGIC HTDYYTLSGA DPEGIFPAIL GHEGAGVVVD VGPGVGTLKK GDHVIPLYTP ECRECKFCLS RKTNLCQKIR ATQGRGLMPD ATSRFSVGGK PIFHYMGTST FSNYIVVPEI AVAKVREDAP FDKICYIGCG VTTGVGAVVY SAKVEAGANV VVFGLGGIGL NVIQGARMVG ADKIIGVDLN PKRVELARKF GMTHFINPNE VENVVDHIVQ LTDGGADYSF ECIGNVKVMR QALECTHKGW GQSFIIGVAA AGEEISTRPF QLVTGREWKG SAFGGARGRT DVPKIVDWYM EGKINIDDLI THTLPLEKIN EGFELMKKGE SIRSVVLY

NT Sequence

ATGAAGACGA AAGCCGCGAT CGCCTGGAAG GCGGGCGAAC CGCTGACGAT CGAAGAGGTC GATCTCGAAG GACCGCGCGC AGGCGAGGTC CTGATCGAAG TGAAGGCCAC CGGCATCTGC CACACCGATT ATTACACCCT CTCGGGCGCA GACCCCGAGG GCATCTTCCC GGCGATCCTC GGGCACGAGG GCGCGGGCGT CGTCGTCGAC GTCGGGCCGG GCGTCGGCAC GCTGAAGAAG GGCGATCACG TGATTCCGCT CTACACGCCC GAATGCCGCG AGTGCAAGTT CTGCCTGTCG CGCAAGACGA ACCTGTGCCA GAAGATCCGC GCGACCCAAG GCCGCGGCCT GATGCCCGAC GCGACGTCGC GCTTCTCGGT CGGCGGCAAG CCGATCTTCC ACTACATGGG CACGTCCACG TTCTCGAACT ACATCGTGGT GCCGGAGATC GCGGTCGCGA AGGTGCGCGA GGACGCGCCG TTCGACAAGA TCTGCTACAT CGGCTGCGGC GTGACGACGG GCGTCGGCGC GGTCGTCTAC TCGGCGAAGG TCGAGGCGGG CGCGAACGTC GTCGTGTTCG GCCTCGGCGG CATCGGCCTG AATGTGATCC AGGGCGCGAG GATGGTGGGC GCGGACAAGA TCATCGGCGT CGATCTCAAC CCGAAGCGCG TCGAGCTCGC GAGGAAGTTC GGCATGACGC ACTTCATCAA CCCGAACGAA GTCGAGAACG TCGTCGACCA CATCGTGCAG TTGACGGACG GCGGCGCCGA TTATTCGTTC GAATGCATCG GCAACGTGAA GGTGATGCGC CAGGCGCTCG AGTGCACGCA CAAGGGGTGG GGGCAGTCGT TCATCATCGG CGTCGCGGCG GCCGGCGAGG AAATCAGCAC GCGGCCGTTC CAGCTCGTGA CCGGCCGCGA GTGGAAGGGC TCCGCGTTCG GCGGCGCGCG CGGGCGCACC GACGTGCCGA AGATCGTCGA CTGGTACATG GAAGGCAAGA TCAACATCGA CGACCTGATC ACGCACACGC TGCCGCTCGA GAAGATCAAC GAAGGCTTCG AGCTGATGAA GAAGGGCGAA TCGATCCGCT CGGTCGTGCT GTAC

Details for BupsA.10611.a.A1.GE27675

HARVESTED ON:	10/13/2009
SEQUENCED ON:	10/21/2009
EXPECTED MW:	41kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

ttagtacagc acgaccgagc ggatcgattc gcccttcttc atcagctcga agccttcgtt gatcttctcg agcggcagcg tgtgcgtgat caggtcgtcg atgttgatct tgccttccat gtaccagtcg acgatcttcg gcacgtcggt gcgcccgcgc gcgccgccga acgcggagcc cttccactcg cggccggtca cgagctggaa cggccgcgtg ctgatttcct cgccggccgc cgcgacgccg atgatgaacg actgccccca ccccttgtgc gtgcactcga gcgcctggcg catcaccttc acgttgccga tgcattcgaa cgaataatcg gcgccgccgt ccgtcaactg cacgatgtgg tcgacgacgt tctcgacttc gttcgggttg atgaagtgcg tcatgccgaa cttcctcgcg agctcgacgc gcttcgggtt gagatcgacg ccgatgatct tgtccgcgcc caccatcctc gcgccctgga tcacattcag gccgatgccg ccgaggccga acacgacgac gttcgcgccc gcctcgacct tcgccgagta gacgaccgcg ccgacgcccg tcgtcacgcc gcagccgatg tagcagatct tgtcgaacgg cgcgtcctcg cgcaccttcg cgaccgcgat ctccggcacc acgatgtagt tcgagaacgt ggacgtgccc atgtagtgga agatcggctt gccgccgacc gagaagcgcg acgtcgcgtc gggcatcagg ccgcggcctt gggtcgcgcg gatcttctgg cacaggttcg tcttgcgcga caggcagaac ttgcactcgc ggcattcggg cgtgtagagc ggaatcacgt gatcgccctt cttcagcgtg ccgacgcccg gcccgacgtc gacgacgacg cccgcgccct cgtgcccgag gatcgccggg aagatgccct cggggtctgc gcccgagagg gtgtaataat cggtgtggca gatgccggtg gccttcactt cgatcaggac ctcgcctgcg cgcggtcctt cgagatcgac ctcttcgatc gtcagcggtt cgcccgcctt ccaggcgatc gcggctttcg tcttcatcga accaggaccc tgggtctgag cttccagggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKTKAAIAW KAGEPLTIEE VDLEGPRAGE VLIEVKATGI CHTDYYTLSG ADPEGIFPAI LGHEGAGVVV DVGPGVGTLK KGDHVIPLYT PECRECKFCL SRKTNLCQKI RATQGRGLMP DATSRFSVGG KPIFHYMGTS TFSNYIVVPE IAVAKVREDA PFDKICYIGC GVTTGVGAVV YSAKVEAGAN VVVFGLGGIG LNVIQGARMV GADKIIGVDL NPKRVELARK FGMTHFINPN EVENVVDHIV QLTDGGADYS FECIGNVKVM RQALECTHKG WGQSFIIGVA AAGEEISTRP FQLVTGREWK GSAFGGARGR TDVPKIVDWY MEGKINIDDL ITHTLPLEKI NEGFELMKKG ESIRSVVLY

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagacgaaa gccgcgatcg cctggaaggc gggcgaaccg ctgacgatcg aagaggtcga tctcgaagga ccgcgcgcag gcgaggtcct gatcgaagtg aaggccaccg gcatctgcca caccgattat tacaccctct cgggcgcaga ccccgagggc atcttcccgg cgatcctcgg gcacgagggc gcgggcgtcg tcgtcgacgt cgggccgggc gtcggcacgc tgaagaaggg cgatcacgtg attccgctct acacgcccga atgccgcgag tgcaagttct gcctgtcgcg caagacgaac ctgtgccaga agatccgcgc gacccaaggc cgcggcctga tgcccgacgc gacgtcgcgc ttctcggtcg gcggcaagcc gatcttccac tacatgggca cgtccacgtt ctcgaactac atcgtggtgc cggagatcgc ggtcgcgaag gtgcgcgagg acgcgccgtt cgacaagatc tgctacatcg gctgcggcgt gacgacgggc gtcggcgcgg tcgtctactc ggcgaaggtc gaggcgggcg cgaacgtcgt cgtgttcggc ctcggcggca tcggcctgaa tgtgatccag ggcgcgagga tggtgggcgc ggacaagatc atcggcgtcg atctcaaccc gaagcgcgtc gagctcgcga ggaagttcgg catgacgcac ttcatcaacc cgaacgaagt cgagaacgtc gtcgaccaca tcgtgcagtt gacggacggc ggcgccgatt attcgttcga atgcatcggc aacgtgaagg tgatgcgcca ggcgctcgag tgcacgcaca aggggtgggg gcagtcgttc atcatcggcg tcgcggcggc cggcgaggaa atcagcacgc ggccgttcca gctcgtgacc ggccgcgagt ggaagggctc cgcgttcggc ggcgcgcgcg ggcgcaccga cgtgccgaag atcgtcgact ggtacatgga aggcaagatc aacatcgacg acctgatcac gcacacgctg ccgctcgaga agatcaacga aggcttcgag ctgatgaaga agggcgaatc gatccgctcg gtcgtgctgt acaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa