BupsA.10537.a

cell division protein FtsA

CENTER ID: BupsA.10537.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10537.a.A1.GE27674 Full length( BupsA.10537.a ) 1 410
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10537.a.A1.PS00449 Full length( BupsA.10537.a ) 1 410

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3685
RefSeq: YP_334910.1
UniProt: Q3JNE3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKDYKDLLV ALDIGTSKVV AVVAELKGEG HYEVIGLGQS ESKGLKKGVV VNIEATVQSI QRALEEAELM ADCKITNVFT GIAGSHIRSF NSSGMVAIKE KEVTQTDVAR VIETAKAINI PTDQQVLHIL TQEFIIDGQE DVREPIGMSG IRLEVKVHIV TGAVSAAQNI VKCVRRCGLE VNDLILQPLA SSLAVLTEDE KDLGVVLVDI GGGTTDIAIF AEGAIRHTAV IPIAGDQITS DIAMALRTPT PDAEDIKVGY GIAKQALADP DEMVEVPGLG ERGPRTLSRQ ALAAVIEPRV EELFSLVQQV VRESGYEELL SSGVVLTGGA AMMPGMVELG EDIFLKPVRI GAPEYAGGLS DVVRNPRYST AMGLLVEGSA QRMRGRKVAV QSGSAGQIFS RMKEWFLSNF X
NT Sequence
ATGAGCAAAG ACTACAAGGA TCTGCTGGTT GCCCTCGACA TCGGCACGTC GAAGGTGGTG GCCGTCGTCG CCGAGCTGAA GGGCGAGGGC CACTACGAGG TCATCGGCCT CGGCCAGAGC GAGTCGAAGG GGCTGAAGAA GGGCGTCGTG GTGAACATCG AGGCCACCGT CCAATCGATT CAGCGCGCGC TCGAGGAAGC CGAGCTGATG GCCGACTGCA AGATCACCAA CGTCTTCACC GGTATCGCCG GCAGCCACAT CCGCAGTTTC AACTCGAGCG GGATGGTCGC GATCAAGGAA AAGGAAGTCA CGCAGACCGA CGTCGCGCGC GTGATCGAGA CCGCGAAGGC GATCAACATT CCGACCGACC AGCAGGTGCT GCACATCCTC ACGCAGGAAT TCATCATCGA CGGCCAGGAG GACGTGCGCG AGCCGATCGG CATGAGCGGC ATCCGCCTCG AGGTGAAGGT GCACATCGTG ACGGGCGCGG TGAGCGCCGC GCAGAACATC GTCAAGTGCG TGCGCCGCTG CGGGCTCGAA GTGAACGACC TGATCCTGCA GCCGCTCGCG TCGTCGCTCG CGGTGCTGAC GGAAGACGAA AAAGACCTGG GCGTGGTGCT TGTCGACATC GGCGGCGGCA CGACCGACAT CGCGATCTTC GCCGAGGGCG CGATTCGCCA CACGGCGGTG ATTCCGATCG CGGGCGACCA GATCACGAGC GACATCGCGA TGGCGCTGCG CACGCCGACG CCCGACGCGG AGGACATCAA GGTCGGCTAC GGGATCGCGA AGCAGGCGCT CGCCGATCCG GACGAGATGG TCGAAGTGCC GGGCCTGGGC GAGCGCGGTC CGCGCACGCT GTCGCGGCAG GCGCTCGCGG CCGTCATCGA GCCGCGCGTC GAGGAGCTGT TCTCGCTCGT GCAGCAGGTC GTGCGCGAGT CCGGTTACGA AGAGTTACTC AGTTCCGGTG TCGTGCTCAC GGGCGGCGCT GCGATGATGC CCGGCATGGT CGAGCTGGGC GAGGACATTT TTCTGAAGCC GGTGCGCATC GGCGCGCCGG AATATGCGGG CGGGCTCTCG GACGTCGTGC GCAATCCGCG CTATTCGACG GCGATGGGGC TGCTCGTCGA GGGCAGCGCT CAACGGATGC GCGGCCGCAA GGTCGCCGTG CAATCCGGCT CCGCGGGGCA GATCTTCTCG CGGATGAAGG AGTGGTTCCT CAGCAATTTC
Details for BupsA.10537.a.A1.GE27674
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 46kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKDYKDLL VALDIGTSKV VAVVAELKGE GHYEVIGLGQ SESKGLKKGV VVNIEATVQS IQRALEEAEL MADCKITNVF TGIAGSHIRS FNSSGMVAIK EKEVTQTDVA RVIETAKAIN IPTDQQVLHI LTQEFIIDGQ EDVREPIGMS GIRLEVKVHI VTGAVSAAQN IVKCVRRCGL EVNDLILQPL ASSLAVLTED EKDLGVVLVD IGGGTTDIAI FAEGAIRHTA VIPIAGDQIT SDIAMALRTP TPDAEDIKVG YGIAKQALAD PDEMVEVPGL GERGPRTLSR QALAAVIEPR VEELFSLVQQ VVRESGYEEL LSSGVVLTGG AAMMPGMVEL GEDIFLKPVR IGAPEYAGGL SDVVRNPRYS TAMGLLVEGS AQRMRGRKVA VQSGSAGQIF SRMKEWFLS
Validated NT Sequence
tgctgaggaa ccactccttc atccgcgaga agatctgccc cgcggagccg gattgcacgg cgaccttgcg gccgcgcatc cgttgagcgc tgccctcgac gagcagcccc atcgccgtcg aatagcgcgg attgcgcacg acgtccgaga gcccgcccgc atattccggc gcgccgatgc gcaccggctt cagaaaaatg tcctcgccca gctcgaccat gccgggcatc atcgcagcgc cgcccgtgag cacgacaccg gaactgagta actcttcgta accggactcg cgcacgacct gctgcacgag cgagaacagc tcctcgacgc gcggctcgat gacggccgcg agcgcctgcc gcgacagcgt gcgcggaccg cgctcgccca ggcccggcac ttcgaccatc tcgtccggat cggcgagcgc ctgcttcgcg atcccgtagc cgaccttgat gtcctccgcg tcgggcgtcg gcgtgcgcag cgccatcgcg atgtcgctcg tgatctggtc gcccgcgatc ggaatcaccg ccgtgtggcg aatcgcgccc tcggcgaaga tcgcgatgtc ggtcgtgccg ccgccgatgt cgacaagcac cacgcccagg tctttttcgt cttccgtcag caccgcgagc gacgacgcga gcggctgcag gatcaggtcg ttcacttcga gcccgcagcg gcgcacgcac ttgacgatgt tctgcgcggc gctcaccgcg cccgtcacga tgtgcacctt cacctcgagg cggatgccgc tcatgccgat cggctcgcgc acgtcctcct ggccgtcgat gatgaattcc tgcgtgagga tgtgcagcac ctgctggtcg gtcggaatgt tgatcgcctt cgcggtctcg atcacgcgcg cgacgtcggt ctgcgtgact tccttttcct tgatcgcgac catcccgctc gagttgaaac tgcggatgtg gctgccggcg ataccggtga agacgttggt gatcttgcag tcggccatca gctcggcttc ctcgagcgcg cgctgaatcg attggacggt ggcctcgatg ttcaccacga cgcccttctt cagccccttc gactcgctct ggccgaggcc gatgacctcg tagtggccct cgcccttcag ctcggcgacg acggccacca ccttcgacgt gccgatgtcg agggcaacca gcagatcctt gtagtctttg ctcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKDYKDLL VALDIGTSKV VAVVAELKGE GHYEVIGLGQ SESKGLKKGV VVNIEATVQS IQRALEEAEL MADCKITNVF TGIAGSHIRS FNSSGMVAIK EKEVTQTDVA RVIETAKAIN IPTDQQVLHI LTQEFIIDGQ EDVREPIGMS GIRLEVKVHI VTGAVSAAQN IVKCVRRCGL EVNDLILQPL ASSLAVLTED EKDLGVVLVD IGGGTTDIAI FAEGAIRHTA VIPIAGDQIT SDIAMALRTP TPDAEDIKVG YGIAKQALAD PDEMVEVPGL GERGPRTLSR QALAAVIEPR VEELFSLVQQ VVRESGYEEL LSSGVVLTGG AAMMPGMVEL GEDIFLKPVR IGAPEYAGGL SDVVRNPRYS TAMGLLVEGS AQRMRGRKVA VQSGSAGQIF SRMKEWFLSN F
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcaaagac tacaaggatc tgctggttgc cctcgacatc ggcacgtcga aggtggtggc cgtcgtcgcc gagctgaagg gcgagggcca ctacgaggtc atcggcctcg gccagagcga gtcgaagggg ctgaagaagg gcgtcgtggt gaacatcgag gccaccgtcc aatcgattca gcgcgcgctc gaggaagccg agctgatggc cgactgcaag atcaccaacg tcttcaccgg tatcgccggc agccacatcc gcagtttcaa ctcgagcggg atggtcgcga tcaaggaaaa ggaagtcacg cagaccgacg tcgcgcgcgt gatcgagacc gcgaaggcga tcaacattcc gaccgaccag caggtgctgc acatcctcac gcaggaattc atcatcgacg gccaggagga cgtgcgcgag ccgatcggca tgagcggcat ccgcctcgag gtgaaggtgc acatcgtgac gggcgcggtg agcgccgcgc agaacatcgt caagtgcgtg cgccgctgcg ggctcgaagt gaacgacctg atcctgcagc cgctcgcgtc gtcgctcgcg gtgctgacgg aagacgaaaa agacctgggc gtggtgcttg tcgacatcgg cggcggcacg accgacatcg cgatcttcgc cgagggcgcg attcgccaca cggcggtgat tccgatcgcg ggcgaccaga tcacgagcga catcgcgatg gcgctgcgca cgccgacgcc cgacgcggag gacatcaagg tcggctacgg gatcgcgaag caggcgctcg ccgatccgga cgagatggtc gaagtgccgg gcctgggcga gcgcggtccg cgcacgctgt cgcggcaggc gctcgcggcc gtcatcgagc cgcgcgtcga ggagctgttc tcgctcgtgc agcaggtcgt gcgcgagtcc ggttacgaag agttactcag ttccggtgtc gtgctcacgg gcggcgctgc gatgatgccc ggcatggtcg agctgggcga ggacattttt ctgaagccgg tgcgcatcgg cgcgccggaa tatgcgggcg ggctctcgga cgtcgtgcgc aatccgcgct attcgacggc gatggggctg ctcgtcgagg gcagcgctca acggatgcgc ggccgcaagg tcgccgtgca atccggctcc gcggggcaga tcttctcgcg gatgaaggag tggttcctca gcaatttcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BupsA.10537.a.A1.PS00449
PURIFICATION DATe: 12/19/2009
CONCENTRATION: 41.9mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSKDYKDLL VALDIGTSKV VAVVAELKGE GHYEVIGLGQ SESKGLKKGV VVNIEATVQS IQRALEEAEL MADCKITNVF TGIAGSHIRS FNSSGMVAIK EKEVTQTDVA RVIETAKAIN IPTDQQVLHI LTQEFIIDGQ EDVREPIGMS GIRLEVKVHI VTGAVSAAQN IVKCVRRCGL EVNDLILQPL ASSLAVLTED EKDLGVVLVD IGGGTTDIAI FAEGAIRHTA VIPIAGDQIT SDIAMALRTP TPDAEDIKVG YGIAKQALAD PDEMVEVPGL GERGPRTLSR QALAAVIEPR VEELFSLVQQ VVRESGYEEL LSSGVVLTGG AAMMPGMVEL GEDIFLKPVR IGAPEYAGGL SDVVRNPRYS TAMGLLVEGS AQRMRGRKVA VQSGSAGQIF SRMKEWFLS
Validated NT Sequence
tgctgaggaa ccactccttc atccgcgaga agatctgccc cgcggagccg gattgcacgg cgaccttgcg gccgcgcatc cgttgagcgc tgccctcgac gagcagcccc atcgccgtcg aatagcgcgg attgcgcacg acgtccgaga gcccgcccgc atattccggc gcgccgatgc gcaccggctt cagaaaaatg tcctcgccca gctcgaccat gccgggcatc atcgcagcgc cgcccgtgag cacgacaccg gaactgagta actcttcgta accggactcg cgcacgacct gctgcacgag cgagaacagc tcctcgacgc gcggctcgat gacggccgcg agcgcctgcc gcgacagcgt gcgcggaccg cgctcgccca ggcccggcac ttcgaccatc tcgtccggat cggcgagcgc ctgcttcgcg atcccgtagc cgaccttgat gtcctccgcg tcgggcgtcg gcgtgcgcag cgccatcgcg atgtcgctcg tgatctggtc gcccgcgatc ggaatcaccg ccgtgtggcg aatcgcgccc tcggcgaaga tcgcgatgtc ggtcgtgccg ccgccgatgt cgacaagcac cacgcccagg tctttttcgt cttccgtcag caccgcgagc gacgacgcga gcggctgcag gatcaggtcg ttcacttcga gcccgcagcg gcgcacgcac ttgacgatgt tctgcgcggc gctcaccgcg cccgtcacga tgtgcacctt cacctcgagg cggatgccgc tcatgccgat cggctcgcgc acgtcctcct ggccgtcgat gatgaattcc tgcgtgagga tgtgcagcac ctgctggtcg gtcggaatgt tgatcgcctt cgcggtctcg atcacgcgcg cgacgtcggt ctgcgtgact tccttttcct tgatcgcgac catcccgctc gagttgaaac tgcggatgtg gctgccggcg ataccggtga agacgttggt gatcttgcag tcggccatca gctcggcttc ctcgagcgcg cgctgaatcg attggacggt ggcctcgatg ttcaccacga cgcccttctt cagccccttc gactcgctct ggccgaggcc gatgacctcg tagtggccct cgcccttcag ctcggcgacg acggccacca ccttcgacgt gccgatgtcg agggcaacca gcagatcctt gtagtctttg ctcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSKDYKDLL VALDIGTSKV VAVVAELKGE GHYEVIGLGQ SESKGLKKGV VVNIEATVQS IQRALEEAEL MADCKITNVF TGIAGSHIRS FNSSGMVAIK EKEVTQTDVA RVIETAKAIN IPTDQQVLHI LTQEFIIDGQ EDVREPIGMS GIRLEVKVHI VTGAVSAAQN IVKCVRRCGL EVNDLILQPL ASSLAVLTED EKDLGVVLVD IGGGTTDIAI FAEGAIRHTA VIPIAGDQIT SDIAMALRTP TPDAEDIKVG YGIAKQALAD PDEMVEVPGL GERGPRTLSR QALAAVIEPR VEELFSLVQQ VVRESGYEEL LSSGVVLTGG AAMMPGMVEL GEDIFLKPVR IGAPEYAGGL SDVVRNPRYS TAMGLLVEGS AQRMRGRKVA VQSGSAGQIF SRMKEWFLSN F
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAAAGAC TACAAGGATC TGCTGGTTGC CCTCGACATC GGCACGTCGA AGGTGGTGGC CGTCGTCGCC GAGCTGAAGG GCGAGGGCCA CTACGAGGTC ATCGGCCTCG GCCAGAGCGA GTCGAAGGGG CTGAAGAAGG GCGTCGTGGT GAACATCGAG GCCACCGTCC AATCGATTCA GCGCGCGCTC GAGGAAGCCG AGCTGATGGC CGACTGCAAG ATCACCAACG TCTTCACCGG TATCGCCGGC AGCCACATCC GCAGTTTCAA CTCGAGCGGG ATGGTCGCGA TCAAGGAAAA GGAAGTCACG CAGACCGACG TCGCGCGCGT GATCGAGACC GCGAAGGCGA TCAACATTCC GACCGACCAG CAGGTGCTGC ACATCCTCAC GCAGGAATTC ATCATCGACG GCCAGGAGGA CGTGCGCGAG CCGATCGGCA TGAGCGGCAT CCGCCTCGAG GTGAAGGTGC ACATCGTGAC GGGCGCGGTG AGCGCCGCGC AGAACATCGT CAAGTGCGTG CGCCGCTGCG GGCTCGAAGT GAACGACCTG ATCCTGCAGC CGCTCGCGTC GTCGCTCGCG GTGCTGACGG AAGACGAAAA AGACCTGGGC GTGGTGCTTG TCGACATCGG CGGCGGCACG ACCGACATCG CGATCTTCGC CGAGGGCGCG ATTCGCCACA CGGCGGTGAT TCCGATCGCG GGCGACCAGA TCACGAGCGA CATCGCGATG GCGCTGCGCA CGCCGACGCC CGACGCGGAG GACATCAAGG TCGGCTACGG GATCGCGAAG CAGGCGCTCG CCGATCCGGA CGAGATGGTC GAAGTGCCGG GCCTGGGCGA GCGCGGTCCG CGCACGCTGT CGCGGCAGGC GCTCGCGGCC GTCATCGAGC CGCGCGTCGA GGAGCTGTTC TCGCTCGTGC AGCAGGTCGT GCGCGAGTCC GGTTACGAAG AGTTACTCAG TTCCGGTGTC GTGCTCACGG GCGGCGCTGC GATGATGCCC GGCATGGTCG AGCTGGGCGA GGACATTTTT CTGAAGCCGG TGCGCATCGG CGCGCCGGAA TATGCGGGCG GGCTCTCGGA CGTCGTGCGC AATCCGCGCT ATTCGACGGC GATGGGGCTG CTCGTCGAGG GCAGCGCTCA ACGGATGCGC GGCCGCAAGG TCGCCGTGCA ATCCGGCTCC GCGGGGCAGA TCTTCTCGCG GATGAAGGAG TGGTTCCTCA GCAATTTCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA