BupsA.10520.a

metallo-beta-lactamase superfamily protein

CENTER ID: BupsA.10520.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10520.a.A1.GE27673 Full length( BupsA.10520.a ) 1 214
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10520.a.A1.PS00442 Full length( BupsA.10520.a ) 1 214

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_127147
PATRIC ID: fig|320372.6.peg.42
RefSeq: YP_331461.1
UniProt: Q3JY92

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKVTLIPVTP FQQNCSLLVC EKTGRAAVVD PGGDLELIEQ EVARQHVQVE KVLLTHGHVD HCAGAKRLAA HYGVPIEGPH EDERFWIEKL PMQSERFGFP AAEAFEPDRW LADGDTLAFG EETLEVYHCP GHTPGHVVFF SRAHRLAIVG DVLFAGSIGR TDFPRGNHAD LIRSIREKLW PLGDDVTFVP GHGPVSTFGD ERRTNPFVAD EVAG
NT Sequence
ATGAAAGTCA CGCTCATTCC GGTCACGCCG TTCCAGCAGA ACTGCTCGCT GCTCGTTTGC GAAAAGACGG GACGCGCCGC CGTCGTCGAT CCGGGCGGCG ATCTCGAACT GATCGAGCAG GAGGTCGCCC GGCAGCACGT GCAGGTCGAG AAGGTGCTGC TCACGCACGG CCACGTCGAC CATTGCGCGG GCGCGAAGCG GCTCGCCGCG CATTACGGCG TGCCGATCGA GGGGCCGCAC GAAGACGAGC GCTTCTGGAT CGAAAAGCTG CCGATGCAAA GCGAGCGCTT CGGCTTTCCG GCTGCCGAAG CGTTCGAGCC GGACCGCTGG CTCGCCGACG GCGACACGCT CGCCTTCGGC GAGGAAACGC TCGAGGTCTA TCACTGCCCT GGGCACACGC CGGGCCACGT CGTATTCTTC AGCCGCGCGC ATCGGCTCGC GATCGTCGGC GACGTGCTGT TCGCCGGTTC GATCGGCCGC ACCGACTTCC CGCGCGGCAA CCACGCGGAC CTGATCCGCT CGATCCGCGA AAAGCTCTGG CCGCTCGGCG ACGACGTCAC GTTCGTGCCC GGCCACGGCC CCGTGTCGAC GTTCGGCGAC GAGCGCCGCA CGAATCCGTT CGTCGCGGAC GAGGTGGCGG GA
Details for BupsA.10520.a.A1.GE27673
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 25kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKVTLIPVT PFQQNCSLLV CEKTGRAAVV DPGGDLELIE QEVARQHVQV EKVLLTHGHV DHCAGAKRLA AHYGVPIEGP HEDERFWIEK LPMQSERFGF PAAEAFEPDR WLADGDTLAF GEETLEVYHC PGHTPGHVVF FSRAHRLAIV GDVLFAGSIG RTDFPRGNHA DLIRSIREKL WPLGDDVTFV PGHGPVSTFG DERRTNPFVA DEVAG
Validated NT Sequence
ttattatccc gccacctcgt ccgcgacgaa cggattcgtg cggcgctcgt cgccgaacgt cgacacgggg ccgtggccgg gcacgaacgt gacgtcgtcg ccgagcggcc agagcttttc gcggatcgag cggatcaggt ccgcgtggtt gccgcgcggg aagtcggtgc ggccgatcga accggcgaac agcacgtcgc cgacgatcgc gagccgatgc gcgcggctga agaatacgac gtggcccggc gtgtgcccag ggcagtgata gacctcgagc gtttcctcgc cgaaggcgag cgtgtcgccg tcggcgagcc agcggtccgg ctcgaacgct tcggcagccg gaaagccgaa gcgctcgctt tgcatcggca gcttttcgat ccagaagcgc tcgtcttcgt gcggcccctc gatcggcacg ccgtaatgcg cggcgagccg cttcgcgccc gcgcaatggt cgacgtggcc gtgcgtgagc agcaccttct cgacctgcac gtgctgccgg gcgacctcct gctcgatcag ttcgagatcg ccgcccggat cgacgacggc ggcgcgtccc gtcttttcgc aaacgagcag cgagcagttc tgctggaacg gcgtgaccgg aatgagcgtg actttcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKVTLIPVT PFQQNCSLLV CEKTGRAAVV DPGGDLELIE QEVARQHVQV EKVLLTHGHV DHCAGAKRLA AHYGVPIEGP HEDERFWIEK LPMQSERFGF PAAEAFEPDR WLADGDTLAF GEETLEVYHC PGHTPGHVVF FSRAHRLAIV GDVLFAGSIG RTDFPRGNHA DLIRSIREKL WPLGDDVTFV PGHGPVSTFG DERRTNPFVA DEVAG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaagtcacg ctcattccgg tcacgccgtt ccagcagaac tgctcgctgc tcgtttgcga aaagacggga cgcgccgccg tcgtcgatcc gggcggcgat ctcgaactga tcgagcagga ggtcgcccgg cagcacgtgc aggtcgagaa ggtgctgctc acgcacggcc acgtcgacca ttgcgcgggc gcgaagcggc tcgccgcgca ttacggcgtg ccgatcgagg ggccgcacga agacgagcgc ttctggatcg aaaagctgcc gatgcaaagc gagcgcttcg gctttccggc tgccgaagcg ttcgagccgg accgctggct cgccgacggc gacacgctcg ccttcggcga ggaaacgctc gaggtctatc actgccctgg gcacacgccg ggccacgtcg tattcttcag ccgcgcgcat cggctcgcga tcgtcggcga cgtgctgttc gccggttcga tcggccgcac cgacttcccg cgcggcaacc acgcggacct gatccgctcg atccgcgaaa agctctggcc gctcggcgac gacgtcacgt tcgtgcccgg ccacggcccc gtgtcgacgt tcggcgacga gcgccgcacg aatccgttcg tcgcggacga ggtggcggga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BupsA.10520.a.A1.PS00442
PURIFICATION DATe: 11/17/2009
CONCENTRATION: 11.6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKVTLIPVT PFQQNCSLLV CEKTGRAAVV DPGGDLELIE QEVARQHVQV EKVLLTHGHV DHCAGAKRLA AHYGVPIEGP HEDERFWIEK LPMQSERFGF PAAEAFEPDR WLADGDTLAF GEETLEVYHC PGHTPGHVVF FSRAHRLAIV GDVLFAGSIG RTDFPRGNHA DLIRSIREKL WPLGDDVTFV PGHGPVSTFG DERRTNPFVA DEVAG
Validated NT Sequence
ttattatccc gccacctcgt ccgcgacgaa cggattcgtg cggcgctcgt cgccgaacgt cgacacgggg ccgtggccgg gcacgaacgt gacgtcgtcg ccgagcggcc agagcttttc gcggatcgag cggatcaggt ccgcgtggtt gccgcgcggg aagtcggtgc ggccgatcga accggcgaac agcacgtcgc cgacgatcgc gagccgatgc gcgcggctga agaatacgac gtggcccggc gtgtgcccag ggcagtgata gacctcgagc gtttcctcgc cgaaggcgag cgtgtcgccg tcggcgagcc agcggtccgg ctcgaacgct tcggcagccg gaaagccgaa gcgctcgctt tgcatcggca gcttttcgat ccagaagcgc tcgtcttcgt gcggcccctc gatcggcacg ccgtaatgcg cggcgagccg cttcgcgccc gcgcaatggt cgacgtggcc gtgcgtgagc agcaccttct cgacctgcac gtgctgccgg gcgacctcct gctcgatcag ttcgagatcg ccgcccggat cgacgacggc ggcgcgtccc gtcttttcgc aaacgagcag cgagcagttc tgctggaacg gcgtgaccgg aatgagcgtg actttcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKVTLIPVT PFQQNCSLLV CEKTGRAAVV DPGGDLELIE QEVARQHVQV EKVLLTHGHV DHCAGAKRLA AHYGVPIEGP HEDERFWIEK LPMQSERFGF PAAEAFEPDR WLADGDTLAF GEETLEVYHC PGHTPGHVVF FSRAHRLAIV GDVLFAGSIG RTDFPRGNHA DLIRSIREKL WPLGDDVTFV PGHGPVSTFG DERRTNPFVA DEVAG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAGTCACG CTCATTCCGG TCACGCCGTT CCAGCAGAAC TGCTCGCTGC TCGTTTGCGA AAAGACGGGA CGCGCCGCCG TCGTCGATCC GGGCGGCGAT CTCGAACTGA TCGAGCAGGA GGTCGCCCGG CAGCACGTGC AGGTCGAGAA GGTGCTGCTC ACGCACGGCC ACGTCGACCA TTGCGCGGGC GCGAAGCGGC TCGCCGCGCA TTACGGCGTG CCGATCGAGG GGCCGCACGA AGACGAGCGC TTCTGGATCG AAAAGCTGCC GATGCAAAGC GAGCGCTTCG GCTTTCCGGC TGCCGAAGCG TTCGAGCCGG ACCGCTGGCT CGCCGACGGC GACACGCTCG CCTTCGGCGA GGAAACGCTC GAGGTCTATC ACTGCCCTGG GCACACGCCG GGCCACGTCG TATTCTTCAG CCGCGCGCAT CGGCTCGCGA TCGTCGGCGA CGTGCTGTTC GCCGGTTCGA TCGGCCGCAC CGACTTCCCG CGCGGCAACC ACGCGGACCT GATCCGCTCG ATCCGCGAAA AGCTCTGGCC GCTCGGCGAC GACGTCACGT TCGTGCCCGG CCACGGCCCC GTGTCGACGT TCGGCGACGA GCGCCGCACG AATCCGTTCG TCGCGGACGA GGTGGCGGGA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA