SSGCID: Target Page

BupsA.10478.b

branched-chain amino acid aminotransferase

CENTER ID:	BupsA.10478.b
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.10478.b.A1.GE27672	Full length( BupsA.10478.b )		1	320

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.10478.b.A1.PW27535	Full length( BupsA.10478.b )		1	320

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.4940
RefSeq:	YP_336051.1
UniProt:	Q3JK52

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRSATTMKSS YVWKNGELVP WEHAQVHVMS HALHYGTSVF EGVRAYEIGE KAAILCGREH FERLLFSCKV ARIASPMSVG QWMEATVETL RANGQRSAYI RPLVYRGAGE SLGLDARQCP AEALLITTPW GAYLGDEALQ QGVDVQVSSW RRNGAGAAST LAKIGGQYVN GQAIVMEAHE NGMSEGIALD TNGFVSEGSG ENVFLVYKNE IFTPSIGSSI LSGITRHCVI RIARDLGYTV TETSIPREML YLADEIFFTG TAVEICPVRS VDRMPVGSGK RGAVTKAIQD QYFGIVRGTH ADKWNWLTPV AVPSRDGARA

NT Sequence

GTGAGGAGCG CAACAACGAT GAAATCGAGC TACGTGTGGA AGAACGGTGA ACTGGTGCCG TGGGAGCATG CGCAGGTGCA TGTGATGAGC CATGCGCTGC ATTACGGGAC GAGTGTGTTC GAAGGCGTGC GCGCGTACGA GATCGGCGAG AAGGCGGCGA TCCTCTGCGG CCGTGAGCAC TTCGAGCGCC TGCTGTTCAG CTGCAAGGTC GCGCGGATTG CGTCGCCGAT GAGCGTCGGG CAATGGATGG AGGCGACGGT CGAGACGCTG CGCGCGAACG GTCAGCGCAG CGCGTACATC CGGCCGCTCG TCTATCGCGG CGCGGGCGAA TCGCTCGGGC TCGACGCGCG ACAGTGCCCG GCCGAGGCGC TGCTCATCAC CACACCGTGG GGCGCGTATC TCGGCGACGA GGCGTTGCAG CAAGGCGTCG ACGTGCAGGT GAGCAGCTGG CGGCGCAACG GCGCGGGAGC GGCAAGCACG CTGGCGAAGA TCGGCGGCCA GTACGTCAAC GGTCAGGCGA TCGTGATGGA GGCGCACGAG AACGGGATGA GCGAAGGCAT CGCGCTCGAT ACGAACGGTT TCGTCAGCGA AGGCAGCGGC GAGAACGTGT TCCTTGTCTA CAAGAACGAA ATCTTCACGC CGTCGATCGG CAGCAGCATC CTGAGCGGCA TCACGCGCCA CTGCGTGATC CGGATCGCGC GCGATCTCGG CTACACCGTG ACCGAAACGA GCATTCCGCG CGAAATGCTC TACCTCGCCG ACGAAATCTT CTTCACGGGC ACCGCCGTCG AGATTTGTCC CGTTCGCTCG GTCGACCGGA TGCCGGTCGG CAGCGGCAAG CGCGGCGCGG TGACGAAGGC GATCCAGGAT CAGTACTTCG GCATCGTGCG CGGCACGCAC GCGGACAAAT GGAACTGGCT TACGCCCGTG GCGGTGCCGT CGCGCGACGG AGCACGGGCA

Details for BupsA.10478.b.A1.GE27672

HARVESTED ON:	10/13/2009
SEQUENCED ON:	10/21/2009
EXPECTED MW:	37kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMVRSATT MKSSYVWKNG ELVPWEHAQV HVMSHALHYG TSVFEGVRAY EIGEKAAILC GREHFERLLF SCKVARIASP MSVGQWMEAT VETLRANGQR SAYIRPLVYR GAGESLGLDA RQCPAEALLI TTPWGAYLGD EALQQGVDVQ VSSWRRNGAG AASTLAKIGG QYVNGQAIVM EAHENGMSEG IALDTNGFVS EGSGENVFLV YKNEIFTPSI GSSILSGITR HCVIRIARDL GYTVTETSIP REMLYLADEI FFTGTAVEIC PVRSVDRMPV GSGKRGAVTK AIQDQYFGIV RGTHADKWNW LTPVAVPSRD GARA

Validated NT Sequence

tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ggagcgcaac aacgatgaaa tcgagctacg tgtggaagaa cggtgaactg gtgccgtggg agcatgcgca ggtgcatgtg atgagccatg cgctgcatta cgggacgagt gtgttcgaag gcgtgcgcgc gtacgagatc ggcgagaagg cggcgatcct ctgcggccgt gagcacttcg agcgcctgct gttcagctgc aaggtcgcgc ggattgcgtc gccgatgagc gtcgggcaat ggatggaggc gacggtcgag acgctgcgcg cgaacggtca gcgcagcgcg tacatccggc cgctcgtcta tcgcggcgcg ggcgaatcgc tcgggctcga cgcgcgacag tgcccggccg aggcgctgct catcaccaca ccgtggggcg cgtatctcgg cgacgaggcg ttgcagcaag gcgtcgacgt gcaggtgagc agctggcggc gcaacggcgc gggagcggca agcacgctgg cgaagatcgg cggccagtac gtcaacggtc aggcgatcgt gatggaggcg cacgagaacg ggatgagcga aggcatcgcg ctcgatacga acggtttcgt cagcgaaggc agcggcgaga acgtgttcct tgtctacaag aacgaaatct tcacgccgtc gatcggcagc agcatcctga gcggcatcac gcgccactgc gtgatccgga tcgcgcgcga tctcggctac accgtgaccg aaacgagcat tccgcgcgaa atgctctacc tcgccgacga aatcttcttc acgggcaccg ccgtcgagat ttgtcccgtt cgctcggtcg accggatgcc ggtcggcagc ggcaagcgcg gcgcggtgac gaaggcgatc caggatcagt acttcggcat cgtgcgcggc acgcacgcgg acaaatggaa ctggcttacg cccgtggcgg tgccgtcgcg cgacggagca cgggcataat aa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVRSATTMK SSYVWKNGEL VPWEHAQVHV MSHALHYGTS VFEGVRAYEI GEKAAILCGR EHFERLLFSC KVARIASPMS VGQWMEATVE TLRANGQRSA YIRPLVYRGA GESLGLDARQ CPAEALLITT PWGAYLGDEA LQQGVDVQVS SWRRNGAGAA STLAKIGGQY VNGQAIVMEA HENGMSEGIA LDTNGFVSEG SGENVFLVYK NEIFTPSIGS SILSGITRHC VIRIARDLGY TVTETSIPRE MLYLADEIFF TGTAVEICPV RSVDRMPVGS GKRGAVTKAI QDQYFGIVRG THADKWNWLT PVAVPSRDGA RA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaggagc gcaacaacga tgaaatcgag ctacgtgtgg aagaacggtg aactggtgcc gtgggagcat gcgcaggtgc atgtgatgag ccatgcgctg cattacggga cgagtgtgtt cgaaggcgtg cgcgcgtacg agatcggcga gaaggcggcg atcctctgcg gccgtgagca cttcgagcgc ctgctgttca gctgcaaggt cgcgcggatt gcgtcgccga tgagcgtcgg gcaatggatg gaggcgacgg tcgagacgct gcgcgcgaac ggtcagcgca gcgcgtacat ccggccgctc gtctatcgcg gcgcgggcga atcgctcggg ctcgacgcgc gacagtgccc ggccgaggcg ctgctcatca ccacaccgtg gggcgcgtat ctcggcgacg aggcgttgca gcaaggcgtc gacgtgcagg tgagcagctg gcggcgcaac ggcgcgggag cggcaagcac gctggcgaag atcggcggcc agtacgtcaa cggtcaggcg atcgtgatgg aggcgcacga gaacgggatg agcgaaggca tcgcgctcga tacgaacggt ttcgtcagcg aaggcagcgg cgagaacgtg ttccttgtct acaagaacga aatcttcacg ccgtcgatcg gcagcagcat cctgagcggc atcacgcgcc actgcgtgat ccggatcgcg cgcgatctcg gctacaccgt gaccgaaacg agcattccgc gcgaaatgct ctacctcgcc gacgaaatct tcttcacggg caccgccgtc gagatttgtc ccgttcgctc ggtcgaccgg atgccggtcg gcagcggcaa gcgcggcgcg gtgacgaagg cgatccagga tcagtacttc ggcatcgtgc gcggcacgca cgcggacaaa tggaactggc ttacgcccgt ggcggtgccg tcgcgcgacg gagcacgggc aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for BupsA.10478.b.A1.PW27535

PURIFICATION DATe:	11/9/2009
CONCENTRATION:	25.58mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGGAGC GCAACAACGA TGAAATCGAG CTACGTGTGG AAGAACGGTG AACTGGTGCC GTGGGAGCAT GCGCAGGTGC ATGTGATGAG CCATGCGCTG CATTACGGGA CGAGTGTGTT CGAAGGCGTG CGCGCGTACG AGATCGGCGA GAAGGCGGCG ATCCTCTGCG GCCGTGAGCA CTTCGAGCGC CTGCTGTTCA GCTGCAAGGT CGCGCGGATT GCGTCGCCGA TGAGCGTCGG GCAATGGATG GAGGCGACGG TCGAGACGCT GCGCGCGAAC GGTCAGCGCA GCGCGTACAT CCGGCCGCTC GTCTATCGCG GCGCGGGCGA ATCGCTCGGG CTCGACGCGC GACAGTGCCC GGCCGAGGCG CTGCTCATCA CCACACCGTG GGGCGCGTAT CTCGGCGACG AGGCGTTGCA GCAAGGCGTC GACGTGCAGG TGAGCAGCTG GCGGCGCAAC GGCGCGGGAG CGGCAAGCAC GCTGGCGAAG ATCGGCGGCC AGTACGTCAA CGGTCAGGCG ATCGTGATGG AGGCGCACGA GAACGGGATG AGCGAAGGCA TCGCGCTCGA TACGAACGGT TTCGTCAGCG AAGGCAGCGG CGAGAACGTG TTCCTTGTCT ACAAGAACGA AATCTTCACG CCGTCGATCG GCAGCAGCAT CCTGAGCGGC ATCACGCGCC ACTGCGTGAT CCGGATCGCG CGCGATCTCG GCTACACCGT GACCGAAACG AGCATTCCGC GCGAAATGCT CTACCTCGCC GACGAAATCT TCTTCACGGG CACCGCCGTC GAGATTTGTC CCGTTCGCTC GGTCGACCGG ATGCCGGTCG GCAGCGGCAA GCGCGGCGCG GTGACGAAGG CGATCCAGGA TCAGTACTTC GGCATCGTGC GCGGCACGCA CGCGGACAAA TGGAACTGGC TTACGCCCGT GGCGGTGCCG TCGCGCGACG GAGCACGGGC AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA