SSGCID: Target Page

BupsA.10201.a

methionyl-tRNA synthetase

CENTER ID:	BupsA.10201.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.10201.a.A1.GE27669	Full length( BupsA.10201.a )		1	725

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.10201.a.A1.PW27532	Full length( BupsA.10201.a )		1	725

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.1223
RefSeq:	YP_332622.1
UniProt:	Q3JUY1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSASDLTSVQ AGAPQGRRQI LVTSALPYAN GQIHIGHLVE YIQTDIWVRT MRMHGHEIYY IGADDTHGTP VMLRAEQEGV SPKQLIERVW REHKRDFDSF GVSFDNFYTT DSDENRVLSE TIYLALKEAG FIAEREIEQA YDPVRQMFLP DRFIKGECPK CHAKDQYGDS CEVCGTTYQP TDLIHPYSVV SGAAPVRKTS THYFFRLSDP RCEAFLREWV SGLAQPEATN KMREWLGEAG EAKLADWDIS RDAPYFGFEI PGAPGKYFYV WLDAPVGYYA SFKNLCERRG LDFDAWIRKD STTEQYHFIG KDILYFHTLF WPAMLEFSGH RTPTNVFAHG FLTVDGAKMS KSRGTFITAQ SYIDTGLNPE WLRYYFAAKL NATMEDIDLN LEDFQARVNS DLVGKYVNIA SRAAGFLLKR FDGRVQASAM NHPLLATLRG AIPQIAAHYE AREYGRALRQ TMELADAVNG YVDSAKPWEL AKDPANAVAL HETCSVSLEA FRLLSLALKP VLPRVAQGVE AFLGIAPLTW ADAGTPLSPE QPVRAYQHLM TRVDPKQIDA LLAANRGSLQ GTAAAAEAGA ANGNGAGSKN GKGAKAAAQP AASAANADDG ASPIISIDDF AKIDLRIAKI VACQAVEGSD KLLQLTLDVG EERTRNVFSG IKSAYRPEQL VGKLTVMVAN LAPRKMKFGL SEGMVLAASA ADEKAEPGLY ILEPHSGAKP GMRVK

NT Sequence

ATGTCCGCAT CCGACCTCAC TTCCGTGCAG GCCGGCGCGC CGCAAGGCCG CCGCCAGATC CTCGTCACGT CCGCGCTGCC CTATGCGAAC GGGCAGATTC ACATCGGCCA TCTGGTCGAG TACATCCAGA CCGACATCTG GGTGCGGACG ATGCGAATGC ACGGTCACGA GATCTACTAC ATCGGCGCGG ACGACACGCA CGGCACGCCC GTCATGCTGC GCGCCGAGCA GGAAGGCGTG AGCCCGAAGC AGTTGATCGA GCGCGTGTGG CGCGAGCACA AGCGCGATTT CGACAGCTTC GGCGTGTCGT TCGACAACTT CTACACGACC GATTCGGACG AGAACCGCGT GCTCAGCGAG ACGATCTATC TCGCGCTGAA GGAGGCGGGC TTCATCGCCG AGCGCGAGAT CGAGCAGGCG TACGACCCCG TCAGGCAAAT GTTCCTGCCG GACCGCTTCA TCAAGGGCGA ATGCCCGAAG TGCCACGCGA AGGACCAGTA CGGCGACAGC TGCGAGGTGT GCGGCACGAC GTACCAGCCG ACGGACCTCA TCCATCCGTA CTCGGTCGTC TCGGGCGCCG CGCCCGTGCG CAAGACGTCG ACGCACTACT TCTTCCGCCT GTCCGATCCG CGCTGCGAGG CGTTCCTGCG CGAATGGGTG AGCGGCCTCG CGCAGCCCGA GGCGACCAAC AAGATGCGCG AATGGCTCGG CGAGGCGGGC GAGGCGAAGC TCGCCGACTG GGACATCTCG CGCGACGCGC CGTACTTCGG CTTCGAGATT CCCGGCGCGC CCGGCAAGTA TTTCTACGTG TGGCTCGACG CGCCCGTCGG CTACTACGCG AGCTTCAAGA ACCTGTGCGA GCGGCGCGGC CTCGATTTCG ACGCGTGGAT CCGCAAGGAT TCGACGACCG AGCAGTACCA CTTCATCGGC AAGGACATCC TGTACTTCCA TACGCTGTTC TGGCCCGCGA TGCTCGAGTT CTCGGGCCAC CGCACGCCGA CCAACGTGTT CGCGCACGGC TTCCTGACGG TCGACGGCGC GAAGATGTCG AAATCGCGCG GCACGTTCAT CACCGCGCAG AGCTACATCG ACACGGGCCT GAACCCCGAA TGGCTGCGCT ACTACTTCGC CGCGAAGCTG AACGCGACGA TGGAAGACAT CGACCTGAAC CTCGAGGATT TCCAGGCGCG CGTCAATAGC GACCTCGTCG GCAAGTACGT GAACATCGCG AGCCGCGCGG CGGGCTTCCT GCTCAAGCGC TTCGACGGCC GCGTGCAGGC GAGCGCGATG AACCATCCGC TTCTCGCGAC GCTGCGCGGC GCGATCCCGC AGATCGCCGC GCACTACGAG GCGCGCGAGT ACGGCCGCGC GCTGCGCCAG ACGATGGAGC TCGCGGATGC GGTGAACGGC TACGTCGACT CCGCGAAGCC GTGGGAGCTC GCGAAGGATC CGGCGAACGC GGTCGCGCTG CATGAAACCT GCAGCGTGAG CCTCGAGGCG TTCCGCCTGC TGTCGCTCGC GCTCAAGCCG GTGCTGCCGC GCGTCGCGCA AGGCGTCGAG GCGTTCCTCG GCATCGCGCC GCTCACGTGG GCCGATGCGG GCACGCCGCT GTCGCCCGAG CAGCCGGTCC GCGCATACCA GCATCTGATG ACCCGCGTCG ATCCGAAGCA GATCGACGCA CTGCTCGCGG CGAACCGCGG CTCGCTGCAA GGCACGGCGG CGGCGGCCGA AGCCGGCGCG GCGAACGGCA ACGGCGCCGG CTCGAAGAAC GGCAAAGGCG CCAAGGCGGC GGCGCAGCCC GCGGCGAGCG CGGCGAACGC CGACGACGGC GCGTCGCCGA TCATCTCGAT CGACGATTTC GCGAAGATCG ACCTGCGCAT CGCGAAGATC GTCGCGTGCC AGGCGGTCGA AGGCTCGGAC AAGCTGCTGC AGCTCACGCT CGACGTCGGC GAGGAGAGGA CCCGCAACGT GTTCTCCGGC ATCAAGTCCG CATACCGGCC CGAGCAGCTC GTCGGCAAGC TGACGGTGAT GGTCGCGAAC CTCGCGCCGC GCAAGATGAA GTTCGGCCTG TCCGAAGGGA TGGTGCTCGC CGCGTCGGCG GCCGACGAGA AGGCCGAGCC CGGCCTCTAC ATCCTCGAGC CGCACAGCGG CGCGAAGCCC GGCATGCGCG TGAAG

Details for BupsA.10201.a.A1.GE27669

HARVESTED ON:	10/13/2009
SEQUENCED ON:	10/21/2009
EXPECTED MW:	82kDa
OBSERVED MW:	80kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	73

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSASDLTSV QAGAPQGRRQ ILVTSALPYA NGQIHIGHLV EYIQTDIWVR TMRMHGHEIY YIGADDTHGT PVMLRAEQEG VSPKQLIERV WREHKRDFDS FGVSFDNFYT TDSDENRVLS ETIYLALKEA GFIAEREIEQ AYDPVRQMFL PDRFIKGECP KCHAKDQYGD SCEVCGTTYQ PTDLIHPYSV VSGAAPVRKT STHYFFRLSD PRCEAFLREW VSGLAQPEAT NKMREWXGEA GEAKLADWDI SRDAPYFGFX IPGAPGKYFY VWLDAPVGYY ASFKNLXERR GLXFDAXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXSVSLE AFRXLSLAXK PVLPRVAQGV EAFLGIAPLT WADAGTPLSP EQPVRAYQHL MTRVDPKQID ALLAANRGSL QGTAAAAEAG AANGNGAGSK NGKGAKAAAQ PAASAANADD GASPIISIDD FAKIDLRIAK IVACQAVEGS DKLLQLTLDV GEERTRNVFS GIKSAYRPEQ LVGKLTVMVA NLAPRKMKFG LSEGMVLAAS AADEKAEPGL YILEPHSGAK PGMRVK

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSASDLTSV QAGAPQGRRQ ILVTSALPYA NGQIHIGHLV EYIQTDIWVR TMRMHGHEIY YIGADDTHGT PVMLRAEQEG VSPKQLIERV WREHKRDFDS FGVSFDNFYT TDSDENRVLS ETIYLALKEA GFIAEREIEQ AYDPVRQMFL PDRFIKGECP KCHAKDQYGD SCEVCGTTYQ PTDLIHPYSV VSGAAPVRKT STHYFFRLSD PRCEAFLREW VSGLAQPEAT NKMREWLGEA GEAKLADWDI SRDAPYFGFE IPGAPGKYFY VWLDAPVGYY ASFKNLCERR GLDFDAWIRK DSTTEQYHFI GKDILYFHTL FWPAMLEFSG HRTPTNVFAH GFLTVDGAKM SKSRGTFITA QSYIDTGLNP EWLRYYFAAK LNATMEDIDL NLEDFQARVN SDLVGKYVNI ASRAAGFLLK RFDGRVQASA MNHPLLATLR GAIPQIAAHY EAREYGRALR QTMELADAVN GYVDSAKPWE LAKDPANAVA LHETCSVSLE AFRLLSLALK PVLPRVAQGV EAFLGIAPLT WADAGTPLSP EQPVRAYQHL MTRVDPKQID ALLAANRGSL QGTAAAAEAG AANGNGAGSK NGKGAKAAAQ PAASAANADD GASPIISIDD FAKIDLRIAK IVACQAVEGS DKLLQLTLDV GEERTRNVFS GIKSAYRPEQ LVGKLTVMVA NLAPRKMKFG LSEGMVLAAS AADEKAEPGL YILEPHSGAK PGMRVK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgcatcc gacctcactt ccgtgcaggc cggcgcgccg caaggccgcc gccagatcct cgtcacgtcc gcgctgccct atgcgaacgg gcagattcac atcggccatc tggtcgagta catccagacc gacatctggg tgcggacgat gcgaatgcac ggtcacgaga tctactacat cggcgcggac gacacgcacg gcacgcccgt catgctgcgc gccgagcagg aaggcgtgag cccgaagcag ttgatcgagc gcgtgtggcg cgagcacaag cgcgatttcg acagcttcgg cgtgtcgttc gacaacttct acacgaccga ttcggacgag aaccgcgtgc tcagcgagac gatctatctc gcgctgaagg aggcgggctt catcgccgag cgcgagatcg agcaggcgta cgaccccgtc aggcaaatgt tcctgccgga ccgcttcatc aagggcgaat gcccgaagtg ccacgcgaag gaccagtacg gcgacagctg cgaggtgtgc ggcacgacgt accagccgac ggacctcatc catccgtact cggtcgtctc gggcgccgcg cccgtgcgca agacgtcgac gcactacttc ttccgcctgt ccgatccgcg ctgcgaggcg ttcctgcgcg aatgggtgag cggcctcgcg cagcccgagg cgaccaacaa gatgcgcgaa tggctcggcg aggcgggcga ggcgaagctc gccgactggg acatctcgcg cgacgcgccg tacttcggct tcgagattcc cggcgcgccc ggcaagtatt tctacgtgtg gctcgacgcg cccgtcggct actacgcgag cttcaagaac ctgtgcgagc ggcgcggcct cgatttcgac gcgtggatcc gcaaggattc gacgaccgag cagtaccact tcatcggcaa ggacatcctg tacttccata cgctgttctg gcccgcgatg ctcgagttct cgggccaccg cacgccgacc aacgtgttcg cgcacggctt cctgacggtc gacggcgcga agatgtcgaa atcgcgcggc acgttcatca ccgcgcagag ctacatcgac acgggcctga accccgaatg gctgcgctac tacttcgccg cgaagctgaa cgcgacgatg gaagacatcg acctgaacct cgaggatttc caggcgcgcg tcaatagcga cctcgtcggc aagtacgtga acatcgcgag ccgcgcggcg ggcttcctgc tcaagcgctt cgacggccgc gtgcaggcga gcgcgatgaa ccatccgctt ctcgcgacgc tgcgcggcgc gatcccgcag atcgccgcgc actacgaggc gcgcgagtac ggccgcgcgc tgcgccagac gatggagctc gcggatgcgg tgaacggcta cgtcgactcc gcgaagccgt gggagctcgc gaaggatccg gcgaacgcgg tcgcgctgca tgaaacctgc agcgtgagcc tcgaggcgtt ccgcctgctg tcgctcgcgc tcaagccggt gctgccgcgc gtcgcgcaag gcgtcgaggc gttcctcggc atcgcgccgc tcacgtgggc cgatgcgggc acgccgctgt cgcccgagca gccggtccgc gcataccagc atctgatgac ccgcgtcgat ccgaagcaga tcgacgcact gctcgcggcg aaccgcggct cgctgcaagg cacggcggcg gcggccgaag ccggcgcggc gaacggcaac ggcgccggct cgaagaacgg caaaggcgcc aaggcggcgg cgcagcccgc ggcgagcgcg gcgaacgccg acgacggcgc gtcgccgatc atctcgatcg acgatttcgc gaagatcgac ctgcgcatcg cgaagatcgt cgcgtgccag gcggtcgaag gctcggacaa gctgctgcag ctcacgctcg acgtcggcga ggagaggacc cgcaacgtgt tctccggcat caagtccgca taccggcccg agcagctcgt cggcaagctg acggtgatgg tcgcgaacct cgcgccgcgc aagatgaagt tcggcctgtc cgaagggatg gtgctcgccg cgtcggcggc cgacgagaag gccgagcccg gcctctacat cctcgagccg cacagcggcg cgaagcccgg catgcgcgtg aagaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BupsA.10201.a.A1.PW27532

PURIFICATION DATe:	3/15/2010
CONCENTRATION:	25.75mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	73

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSASDLTSV QAGAPQGRRQ ILVTSALPYA NGQIHIGHLV EYIQTDIWVR TMRMHGHEIY YIGADDTHGT PVMLRAEQEG VSPKQLIERV WREHKRDFDS FGVSFDNFYT TDSDENRVLS ETIYLALKEA GFIAEREIEQ AYDPVRQMFL PDRFIKGECP KCHAKDQYGD SCEVCGTTYQ PTDLIHPYSV VSGAAPVRKT STHYFFRLSD PRCEAFLREW VSGLAQPEAT NKMREWLGEA GEAKLADWDI SRDAPYFGFE IPGAPGKYFY VWLDAPVGYY ASFKNLCERR GLDFDAWIRK DSTTEQYHFI GKDILYFHTL FWPAMLEFSG HRTPTNVFAH GFLTVDGAKM SKSRGTFITA QSYIDTGLNP EWLRYYFAAK LNATMEDIDL NLEDFQARVN SDLVGKYVNI ASRAAGFLLK RFDGRVQASA MNHPLLATLR GAIPQIAAHY EAREYGRALR QTMELADAVN GYVDSAKPWE LAKDPANAVA LHETCSVSLE AFRLLSLALK PVLPRVAQGV EAFLGIAPLT WADAGTPLSP EQPVRAYQHL MTRVDPKQID ALLAANRGSL QGTAAAAEAG AANGNGAGSK NGKGAKAAAQ PAASAANADD GASPIISIDD FAKIDLRIAK IVACQAVEGS DKLLQLTLDV GEERTRNVFS GIKSAYRPEQ LVGKLTVMVA NLAPRKMKFG LSEGMVLAAS AADEKAEPGL YILEPHSGAK PGMRVK

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCGCATCC GACCTCACTT CCGTGCAGGC CGGCGCGCCG CAAGGCCGCC GCCAGATCCT CGTCACGTCC GCGCTGCCCT ATGCGAACGG GCAGATTCAC ATCGGCCATC TGGTCGAGTA CATCCAGACC GACATCTGGG TGCGGACGAT GCGAATGCAC GGTCACGAGA TCTACTACAT CGGCGCGGAC GACACGCACG GCACGCCCGT CATGCTGCGC GCCGAGCAGG AAGGCGTGAG CCCGAAGCAG TTGATCGAGC GCGTGTGGCG CGAGCACAAG CGCGATTTCG ACAGCTTCGG CGTGTCGTTC GACAACTTCT ACACGACCGA TTCGGACGAG AACCGCGTGC TCAGCGAGAC GATCTATCTC GCGCTGAAGG AGGCGGGCTT CATCGCCGAG CGCGAGATCG AGCAGGCGTA CGACCCCGTC AGGCAAATGT TCCTGCCGGA CCGCTTCATC AAGGGCGAAT GCCCGAAGTG CCACGCGAAG GACCAGTACG GCGACAGCTG CGAGGTGTGC GGCACGACGT ACCAGCCGAC GGACCTCATC CATCCGTACT CGGTCGTCTC GGGCGCCGCG CCCGTGCGCA AGACGTCGAC GCACTACTTC TTCCGCCTGT CCGATCCGCG CTGCGAGGCG TTCCTGCGCG AATGGGTGAG CGGCCTCGCG CAGCCCGAGG CGACCAACAA GATGCGCGAA TGGCTCGGCG AGGCGGGCGA GGCGAAGCTC GCCGACTGGG ACATCTCGCG CGACGCGCCG TACTTCGGCT TCGAGATTCC CGGCGCGCCC GGCAAGTATT TCTACGTGTG GCTCGACGCG CCCGTCGGCT ACTACGCGAG CTTCAAGAAC CTGTGCGAGC GGCGCGGCCT CGATTTCGAC GCGTGGATCC GCAAGGATTC GACGACCGAG CAGTACCACT TCATCGGCAA GGACATCCTG TACTTCCATA CGCTGTTCTG GCCCGCGATG CTCGAGTTCT CGGGCCACCG CACGCCGACC AACGTGTTCG CGCACGGCTT CCTGACGGTC GACGGCGCGA AGATGTCGAA ATCGCGCGGC ACGTTCATCA CCGCGCAGAG CTACATCGAC ACGGGCCTGA ACCCCGAATG GCTGCGCTAC TACTTCGCCG CGAAGCTGAA CGCGACGATG GAAGACATCG ACCTGAACCT CGAGGATTTC CAGGCGCGCG TCAATAGCGA CCTCGTCGGC AAGTACGTGA ACATCGCGAG CCGCGCGGCG GGCTTCCTGC TCAAGCGCTT CGACGGCCGC GTGCAGGCGA GCGCGATGAA CCATCCGCTT CTCGCGACGC TGCGCGGCGC GATCCCGCAG ATCGCCGCGC ACTACGAGGC GCGCGAGTAC GGCCGCGCGC TGCGCCAGAC GATGGAGCTC GCGGATGCGG TGAACGGCTA CGTCGACTCC GCGAAGCCGT GGGAGCTCGC GAAGGATCCG GCGAACGCGG TCGCGCTGCA TGAAACCTGC AGCGTGAGCC TCGAGGCGTT CCGCCTGCTG TCGCTCGCGC TCAAGCCGGT GCTGCCGCGC GTCGCGCAAG GCGTCGAGGC GTTCCTCGGC ATCGCGCCGC TCACGTGGGC CGATGCGGGC ACGCCGCTGT CGCCCGAGCA GCCGGTCCGC GCATACCAGC ATCTGATGAC CCGCGTCGAT CCGAAGCAGA TCGACGCACT GCTCGCGGCG AACCGCGGCT CGCTGCAAGG CACGGCGGCG GCGGCCGAAG CCGGCGCGGC GAACGGCAAC GGCGCCGGCT CGAAGAACGG CAAAGGCGCC AAGGCGGCGG CGCAGCCCGC GGCGAGCGCG GCGAACGCCG ACGACGGCGC GTCGCCGATC ATCTCGATCG ACGATTTCGC GAAGATCGAC CTGCGCATCG CGAAGATCGT CGCGTGCCAG GCGGTCGAAG GCTCGGACAA GCTGCTGCAG CTCACGCTCG ACGTCGGCGA GGAGAGGACC CGCAACGTGT TCTCCGGCAT CAAGTCCGCA TACCGGCCCG AGCAGCTCGT CGGCAAGCTG ACGGTGATGG TCGCGAACCT CGCGCCGCGC AAGATGAAGT TCGGCCTGTC CGAAGGGATG GTGCTCGCCG CGTCGGCGGC CGACGAGAAG GCCGAGCCCG GCCTCTACAT CCTCGAGCCG CACAGCGGCG CGAAGCCCGG CATGCGCGTG AAGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA