SSGCID: Target Page

BupsA.10154.e

Acetylpolyamine aminohydrolase

CENTER ID:	BupsA.10154.e
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.10154.e.A1.GE27668	Full length( BupsA.10154.e )		1	340

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.4234
RefSeq:	YP_335409.1
UniProt:	Q3JLZ4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLTYFHPDQL KHSPLTYLSR GKMRAPHEVP ERAVRLLGAV TSLGFDVRAP EDFGAAPLAA VHSEPYLRFL ADAHREWRRI PEDWGPEVMS NIYVREPNPL RGVLAQAGYY LADGSCPIGE HTYRAAYWSA QSALAGAAAL GRGARDAYAL CRPPGHHACR DAAGGFCYLN NAAIAAQALR ARHARVAILD TDMHHGQGIQ TLFYDRDDVL YVSIHGDPTN FYPAVTGYET ERGAGRGEGF NVNLPMPHGS PEAVFFAQLE RARSRVERFA PDVLVFALGF DVYREDPQSK VAVTTEGFGR LGALVGGLRV PTLIVQEGGY HLETLERNAA ALFRGYEAAR

NT Sequence

ATGCTGACTT ATTTTCATCC GGACCAACTG AAGCACAGTC CGCTCACTTA CCTGTCGCGC GGCAAGATGC GCGCGCCGCA CGAAGTGCCC GAGCGAGCCG TGCGGCTGCT CGGCGCGGTG ACCTCGCTCG GCTTCGACGT GCGCGCGCCC GAGGATTTCG GCGCGGCGCC GCTCGCGGCC GTGCACAGCG AGCCGTATCT GCGCTTTCTC GCCGACGCGC ATCGCGAGTG GCGGCGCATC CCGGAGGACT GGGGGCCCGA GGTGATGTCG AACATCTACG TGCGCGAGCC AAATCCGCTG CGCGGCGTGC TCGCGCAGGC GGGGTACTAT CTCGCGGACG GCAGTTGCCC GATCGGCGAG CACACGTATC GCGCCGCGTA CTGGTCCGCG CAGAGCGCGC TCGCCGGCGC GGCGGCGCTC GGCCGCGGCG CGCGCGATGC GTACGCGCTC TGCCGCCCGC CCGGCCATCA TGCGTGCCGC GACGCGGCGG GCGGCTTCTG CTATCTGAAC AACGCGGCGA TCGCCGCGCA GGCGTTGCGC GCGCGCCATG CGCGCGTGGC GATCCTCGAT ACCGACATGC ATCACGGCCA GGGCATCCAG ACGCTGTTCT ACGATCGCGA CGACGTGCTG TACGTGTCGA TTCACGGCGA TCCGACGAAC TTCTATCCGG CCGTCACCGG TTACGAAACG GAGCGCGGGG CGGGGCGCGG CGAGGGCTTC AACGTGAACC TGCCGATGCC GCACGGCTCG CCCGAAGCGG TGTTCTTCGC GCAGCTCGAG CGCGCGAGGT CGCGCGTCGA GCGCTTCGCG CCGGACGTGC TGGTGTTCGC GCTCGGCTTC GACGTGTATC GCGAGGACCC GCAGTCGAAG GTCGCGGTGA CGACCGAAGG CTTCGGGCGG CTCGGCGCGC TCGTCGGCGG CCTGCGGGTG CCGACGCTGA TCGTGCAGGA AGGCGGCTAT CACCTCGAGA CGCTCGAGCG CAACGCCGCC GCGCTGTTTC GCGGTTACGA GGCGGCGCGT

Details for BupsA.10154.e.A1.GE27668

HARVESTED ON:	10/13/2009
SEQUENCED ON:	10/21/2009
EXPECTED MW:	39kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEAQTQG PGSMLTYFHP DQLKHSPLTY LSRGKMRAPH EVPERAVRLL GAVTSLGFDV RAPEDFGAAP LAAVHSEPYL RFLADAHREW RRIPEDWGPE VMSNIYVREP NPLRGVLAQA GYYLADGSCP IGEHTYRAAY WSAQSALAGA AALGRGARDA YALCRPPGHH ACRDAAGGFC YLNNAAIAAQ ALRARHARVA ILDTDMHHGQ GIQTLFYDRD DVLYVSIHGD PTNFYPAVTG YETERGAGRG EGFNVNLPMP HGSPEAVFFA QLERARSRVE RFAPDVLVFA LGFDVYREDP QSKVAVTTEG FGRLGALVGG LRVPTLIVQE GGYHLETLER NAAALFRG

Validated NT Sequence

ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgc tgacttattt tcatccggac caactgaagc acagtccgct cacttacctg tcgcgcggca agatgcgcgc gccgcacgaa gtgcccgagc gagccgtgcg gctgctcggc gcggtgacct cgctcggctt cgacgtgcgc gcgcccgagg atttcggcgc ggcgccgctc gcggccgtgc acagcgagcc gtatctgcgc tttctcgccg acgcgcatcg cgagtggcgg cgcatcccgg aggactgggg gcccgaggtg atgtcgaaca tctacgtgcg cgagccaaat ccgctgcgcg gcgtgctcgc gcaggcgggg tactatctcg cggacggcag ttgcccgatc ggcgagcaca cgtatcgcgc cgcgtactgg tccgcgcaga gcgcgctcgc cggcgcggcg gcgctcggcc gcggcgcgcg cgatgcgtac gcgctctgcc gcccgcccgg ccatcatgcg tgccgcgacg cggcgggcgg cttctgctat ctgaacaacg cggcgatcgc cgcgcaggcg ttgcgcgcgc gccatgcgcg cgtggcgatc ctcgataccg acatgcatca cggccagggc atccagacgc tgttctacga tcgcgacgac gtgctgtacg tgtcgattca cggcgatccg acgaacttct atccggccgt caccggttac gaaacggagc gcggggcggg gcgcggcgag ggcttcaacg tgaacctgcc gatgccgcac ggctcgcccg aagcggtgtt cttcgcgcag ctcgagcgcg cgaggtcgcg cgtcgagcgc ttcgcgccgg acgtgctggt gttcgcgctc ggcttcgacg tgtatcgcga ggacccgcag tcgaaggtcg cggtgacgac cgaaggcttc gggcggctcg gcgcgctcgt cggcggcctg cgggtgccga cgctgatcgt gcaggaaggc ggctatcacc tcgagacgct cgagcgcaac gccgccgcgc tgtttcgcgg tt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLTYFHPDQ LKHSPLTYLS RGKMRAPHEV PERAVRLLGA VTSLGFDVRA PEDFGAAPLA AVHSEPYLRF LADAHREWRR IPEDWGPEVM SNIYVREPNP LRGVLAQAGY YLADGSCPIG EHTYRAAYWS AQSALAGAAA LGRGARDAYA LCRPPGHHAC RDAAGGFCYL NNAAIAAQAL RARHARVAIL DTDMHHGQGI QTLFYDRDDV LYVSIHGDPT NFYPAVTGYE TERGAGRGEG FNVNLPMPHG SPEAVFFAQL ERARSRVERF APDVLVFALG FDVYREDPQS KVAVTTEGFG RLGALVGGLR VPTLIVQEGG YHLETLERNA AALFRGYEAA R

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgacttat tttcatccgg accaactgaa gcacagtccg ctcacttacc tgtcgcgcgg caagatgcgc gcgccgcacg aagtgcccga gcgagccgtg cggctgctcg gcgcggtgac ctcgctcggc ttcgacgtgc gcgcgcccga ggatttcggc gcggcgccgc tcgcggccgt gcacagcgag ccgtatctgc gctttctcgc cgacgcgcat cgcgagtggc ggcgcatccc ggaggactgg gggcccgagg tgatgtcgaa catctacgtg cgcgagccaa atccgctgcg cggcgtgctc gcgcaggcgg ggtactatct cgcggacggc agttgcccga tcggcgagca cacgtatcgc gccgcgtact ggtccgcgca gagcgcgctc gccggcgcgg cggcgctcgg ccgcggcgcg cgcgatgcgt acgcgctctg ccgcccgccc ggccatcatg cgtgccgcga cgcggcgggc ggcttctgct atctgaacaa cgcggcgatc gccgcgcagg cgttgcgcgc gcgccatgcg cgcgtggcga tcctcgatac cgacatgcat cacggccagg gcatccagac gctgttctac gatcgcgacg acgtgctgta cgtgtcgatt cacggcgatc cgacgaactt ctatccggcc gtcaccggtt acgaaacgga gcgcggggcg gggcgcggcg agggcttcaa cgtgaacctg ccgatgccgc acggctcgcc cgaagcggtg ttcttcgcgc agctcgagcg cgcgaggtcg cgcgtcgagc gcttcgcgcc ggacgtgctg gtgttcgcgc tcggcttcga cgtgtatcgc gaggacccgc agtcgaaggt cgcggtgacg accgaaggct tcgggcggct cggcgcgctc gtcggcggcc tgcgggtgcc gacgctgatc gtgcaggaag gcggctatca cctcgagacg ctcgagcgca acgccgccgc gctgtttcgc ggttacgagg cggcgcgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa