SSGCID: Target Page

BupsA.10154.d

histone deacetylase family protein

CENTER ID:	BupsA.10154.d
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.10154.d.A1.GE27667	Full length( BupsA.10154.d )		1	307

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.10154.d.A1.PS00413	Full length( BupsA.10154.d )		1	307

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|320372.6.peg.3112
RefSeq:	YP_334361.1
UniProt:	Q3JPZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPTAFYTHPD CLLHEMGEWH PESPARLSAI QDQLIASRID DLIVHETAPF ASETALARVH TQAHIDYIRS RIPKEGYVEI DPDTSLNRYT WRAALRAAGA AVEATDAVLE GRYDNAFCSV RPPGHHAEPA RAMGFCFFNN VAIAARHALE IHGLERVAII DFDVHHGNGT EAAFANDERV LMCSFFQHPL YPFSGVDHQA PNMVNLPMAA RTNGMAVREA VDLMWLPRLD AFRPQMLFVS AGFDAHREDD IGGMGLVEAD YEWLTQQICE VARRHAQGRI VSCLEGGYNL SALGRSVVAH LRVLAGV

NT Sequence

ATGCCAACGG CCTTTTACAC GCACCCCGAC TGCCTGCTGC ACGAGATGGG GGAGTGGCAT CCCGAGTCGC CGGCGCGGCT GTCGGCGATT CAGGATCAAC TGATCGCGAG CCGCATCGAC GACCTGATCG TCCACGAGAC GGCGCCGTTC GCGAGCGAGA CGGCGCTGGC CCGCGTGCAT ACGCAGGCGC ACATCGATTA CATCCGCAGC CGGATTCCGA AGGAAGGGTA CGTCGAGATC GATCCCGACA CGTCGCTGAA CCGCTACACA TGGCGCGCGG CGCTGCGCGC GGCCGGCGCG GCCGTCGAGG CGACGGACGC GGTGCTCGAA GGCCGCTACG ACAACGCGTT CTGCAGCGTG CGCCCGCCGG GCCATCATGC GGAACCCGCG CGCGCGATGG GCTTCTGTTT CTTCAACAAC GTCGCGATCG CCGCGCGCCA CGCGCTCGAG ATCCACGGCC TCGAGCGCGT CGCGATCATC GATTTCGACG TCCATCACGG CAACGGCACC GAGGCCGCGT TCGCGAACGA CGAGCGCGTG CTGATGTGCA GCTTCTTCCA GCATCCGCTG TATCCGTTTT CGGGCGTCGA CCACCAGGCG CCGAACATGG TCAATCTGCC GATGGCCGCG CGCACCAACG GGATGGCGGT GCGCGAGGCG GTCGATCTGA TGTGGCTGCC GCGGCTCGAT GCGTTCAGGC CGCAGATGCT GTTCGTGTCG GCGGGGTTCG ATGCGCATCG CGAGGACGAC ATCGGCGGGA TGGGGCTCGT CGAGGCCGAC TACGAGTGGC TCACGCAGCA GATCTGCGAA GTCGCGCGGC GGCATGCGCA AGGCCGGATC GTGAGCTGCC TCGAAGGCGG CTACAACCTG TCCGCGCTCG GGCGAAGCGT GGTCGCGCAT CTGCGCGTGC TCGCCGGCGT G

Details for BupsA.10154.d.A1.GE27667

HARVESTED ON:	10/13/2009
SEQUENCED ON:	10/21/2009
EXPECTED MW:	36kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	26
PERCENT COVERAGE:	97

Validated AA Sequence

MRDHASPERG QVVAAFEAAH DPALRMPPRD FADLLREPLV VGLDEPHPAD VVLAMRIEPR RHEQHLRPER IEPRQPHQID RLAHRHPVGA RGHRQIDHVR RLVVDARKRI QRMLEEAAHQ HALVVRERGL GAVAVMDVEI DDRDALEAVD LERVARGDRD VVEETEAHRA RGFRMMARRA HAAERVVVAA FEHRVRRLDG RAGRAQRRAP CVAVQRRVGI DLDVPFLRNP AADVIDVRLR MHAGQRRLAR ERRRLVDDQV VDAARDQLIL NRRQPRRRLG MPLPHLVQQA VGVRVKGRWH RTRTLGLSFQ GTHMMVMVMS

Validated NT Sequence

anggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccaa cggcctttta cacgcacccc gactgcctgc tgcacgagat gggggagtgg catcccgagt cgccggcgcg gctgtcggcg attcaggatc aactgatcgc gagccgcatc gacgacctga tcgtccacga gacggcgccg ttcgcgagcg agacggcgct ggcccgcgtg catacgcagg cgcacatcga ttacatccgc agccggattc cgaaggaagg gtacgtcgag atcgatcccg acacgtcgct gaaccgctac acatggcgcg cggcgctgcg cgcggccggc gcggccgtcg aggcgacgga cgcggtgctc gaaggccgct acgacaacgc gttctgcagc gtgcgcccgc cgggccatca tgcggaaccc gcgcgcgcga tgggcttctg tttcttcaac aacgtcgcga tcgccgcgcg ccacgcgctc gagatccacg gcctcgagcg cgtcgcgatc atcgatttcg acgtccatca cggcaacggc accgaggccg cgttcgcgaa cgacgagcgc gtgctgatgt gcagcttctt ccagcatccg ctgtatccgt tttcgggcgt cgaccaccag gcgccgaaca tggtcaatct gccgatggcc gcgcgcacca acgggatggc ggtgcgcgag gcggtcgatc tgatgtggct gccgcggctc gatgcgttca ggccgcagat gctgttcgtg tcggcggggt tcgatgcgca tcgcgaggac gacatcggcg ggatggggct cgtcgaggcc gactacgagt ggctcacgca gcagatctgc gaagtcgcgc ggcggcatgc gcaaggccgg atcgtgagct gcctcgaagg cggctacaac ctgtccgcgc tcgggcgaag cgtggtcgcg catctgcgcg tgctcgccgg cgtgtaataa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPTAFYTHP DCLLHEMGEW HPESPARLSA IQDQLIASRI DDLIVHETAP FASETALARV HTQAHIDYIR SRIPKEGYVE IDPDTSLNRY TWRAALRAAG AAVEATDAVL EGRYDNAFCS VRPPGHHAEP ARAMGFCFFN NVAIAARHAL EIHGLERVAI IDFDVHHGNG TEAAFANDER VLMCSFFQHP LYPFSGVDHQ APNMVNLPMA ARTNGMAVRE AVDLMWLPRL DAFRPQMLFV SAGFDAHRED DIGGMGLVEA DYEWLTQQIC EVARRHAQGR IVSCLEGGYN LSALGRSVVA HLRVLAGV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccaacggcc ttttacacgc accccgactg cctgctgcac gagatggggg agtggcatcc cgagtcgccg gcgcggctgt cggcgattca ggatcaactg atcgcgagcc gcatcgacga cctgatcgtc cacgagacgg cgccgttcgc gagcgagacg gcgctggccc gcgtgcatac gcaggcgcac atcgattaca tccgcagccg gattccgaag gaagggtacg tcgagatcga tcccgacacg tcgctgaacc gctacacatg gcgcgcggcg ctgcgcgcgg ccggcgcggc cgtcgaggcg acggacgcgg tgctcgaagg ccgctacgac aacgcgttct gcagcgtgcg cccgccgggc catcatgcgg aacccgcgcg cgcgatgggc ttctgtttct tcaacaacgt cgcgatcgcc gcgcgccacg cgctcgagat ccacggcctc gagcgcgtcg cgatcatcga tttcgacgtc catcacggca acggcaccga ggccgcgttc gcgaacgacg agcgcgtgct gatgtgcagc ttcttccagc atccgctgta tccgttttcg ggcgtcgacc accaggcgcc gaacatggtc aatctgccga tggccgcgcg caccaacggg atggcggtgc gcgaggcggt cgatctgatg tggctgccgc ggctcgatgc gttcaggccg cagatgctgt tcgtgtcggc ggggttcgat gcgcatcgcg aggacgacat cggcgggatg gggctcgtcg aggccgacta cgagtggctc acgcagcaga tctgcgaagt cgcgcggcgg catgcgcaag gccggatcgt gagctgcctc gaaggcggct acaacctgtc cgcgctcggg cgaagcgtgg tcgcgcatct gcgcgtgctc gccggcgtga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for BupsA.10154.d.A1.PS00413

PURIFICATION DATe:	11/25/2009
CONCENTRATION:	38.93mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	100µl
PERCENT IDENTITY:	26
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCAACGGCC TTTTACACGC ACCCCGACTG CCTGCTGCAC GAGATGGGGG AGTGGCATCC CGAGTCGCCG GCGCGGCTGT CGGCGATTCA GGATCAACTG ATCGCGAGCC GCATCGACGA CCTGATCGTC CACGAGACGG CGCCGTTCGC GAGCGAGACG GCGCTGGCCC GCGTGCATAC GCAGGCGCAC ATCGATTACA TCCGCAGCCG GATTCCGAAG GAAGGGTACG TCGAGATCGA TCCCGACACG TCGCTGAACC GCTACACATG GCGCGCGGCG CTGCGCGCGG CCGGCGCGGC CGTCGAGGCG ACGGACGCGG TGCTCGAAGG CCGCTACGAC AACGCGTTCT GCAGCGTGCG CCCGCCGGGC CATCATGCGG AACCCGCGCG CGCGATGGGC TTCTGTTTCT TCAACAACGT CGCGATCGCC GCGCGCCACG CGCTCGAGAT CCACGGCCTC GAGCGCGTCG CGATCATCGA TTTCGACGTC CATCACGGCA ACGGCACCGA GGCCGCGTTC GCGAACGACG AGCGCGTGCT GATGTGCAGC TTCTTCCAGC ATCCGCTGTA TCCGTTTTCG GGCGTCGACC ACCAGGCGCC GAACATGGTC AATCTGCCGA TGGCCGCGCG CACCAACGGG ATGGCGGTGC GCGAGGCGGT CGATCTGATG TGGCTGCCGC GGCTCGATGC GTTCAGGCCG CAGATGCTGT TCGTGTCGGC GGGGTTCGAT GCGCATCGCG AGGACGACAT CGGCGGGATG GGGCTCGTCG AGGCCGACTA CGAGTGGCTC ACGCAGCAGA TCTGCGAAGT CGCGCGGCGG CATGCGCAAG GCCGGATCGT GAGCTGCCTC GAAGGCGGCT ACAACCTGTC CGCGCTCGGG CGAAGCGTGG TCGCGCATCT GCGCGTGCTC GCCGGCGTGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A