BupsA.10154.c

histone deacetylase family protein

CENTER ID: BupsA.10154.c
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10154.c.A1.GE27666 Full length( BupsA.10154.c ) 1 340
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.2604
RefSeq: YP_333884.1
UniProt: Q3JRB9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQPPSSAGYA ESPDSKRRLL SLVHASGLAA KLDMSSAPAA TDDDLRRIHP AHYLDAFKRA SDAGGGDLGE LAPFGRGSYE IAALSAGLAL AAVDAVLAER TANAFSLSRP PGHHCLRDKP MGFCLLANIP IAIEAARAKH RVERVAVIDW DVHHGNGTQS IYYDDPNTLT ISLHQDRCFP PGYSGADERG AGAGAGSNVN VPLLAGAGDD AYRYAFERIV LPALDAFRPE LVIVASGLDA NAVDPLARMQ LHSDSYRYMT HALKQAAQRH CGGRLVIVHE GGYSEAYVPF CGHAIVEALA GMRTDVADPM LELAIAQQPG ERFNAFQRQL IDEMATSFGY
NT Sequence
GTGCAGCCGC CGTCGAGCGC GGGCTATGCG GAATCGCCCG ATTCGAAGCG GCGCCTGCTG TCGCTCGTGC ACGCGTCCGG GCTCGCGGCG AAACTCGACA TGTCGAGCGC GCCCGCCGCG ACCGACGACG ATCTGCGGCG CATCCACCCC GCGCACTACC TCGACGCGTT CAAGCGCGCG AGCGACGCGG GTGGCGGCGA TCTCGGCGAA CTCGCGCCGT TCGGCCGTGG CAGCTACGAG ATCGCGGCGC TATCCGCGGG GCTCGCGCTC GCCGCCGTCG ACGCGGTGCT CGCCGAGCGC ACGGCCAACG CGTTCTCGCT GTCGCGCCCG CCCGGCCATC ACTGCCTGCG TGACAAGCCG ATGGGTTTTT GCCTGCTCGC GAACATTCCG ATCGCGATCG AGGCCGCGCG CGCGAAACAT CGCGTCGAGC GCGTCGCGGT GATCGACTGG GACGTGCATC ACGGCAACGG CACGCAGTCG ATCTACTACG ACGATCCGAA CACGCTGACG ATCTCGCTGC ATCAGGACCG CTGCTTTCCG CCCGGCTACA GCGGCGCCGA CGAACGCGGC GCGGGCGCGG GTGCGGGCTC GAACGTCAAC GTCCCGCTCC TCGCGGGCGC CGGCGACGAC GCGTATCGAT ACGCATTCGA GCGAATCGTG CTGCCCGCGC TCGATGCGTT CCGGCCGGAG CTCGTCATCG TCGCGAGCGG GCTCGACGCG AATGCGGTCG ACCCGCTCGC GCGGATGCAA CTGCACAGCG ACAGCTACCG GTACATGACG CATGCGCTGA AGCAGGCCGC GCAGCGGCAC TGCGGGGGAC GGCTCGTCAT CGTGCACGAG GGCGGTTATT CGGAGGCCTA CGTACCGTTT TGCGGGCATG CGATCGTCGA GGCACTGGCG GGCATGCGCA CCGACGTCGC CGATCCGATG CTCGAGCTCG CGATCGCGCA ACAGCCCGGC GAGCGTTTCA ACGCATTCCA GCGGCAACTG ATCGACGAAA TGGCGACGAG CTTCGGTTAC
Details for BupsA.10154.c.A1.GE27666
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 38kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MVQPPSSAGY AESPDSKRRL LSLVHASGLA AKLDMSSAPA ATDDDLRRIH PAHYLDAFKR ASDAGGGDLG ELAPFGRGSY EIAALSAGLA LAAVDAVLAE RTANAFSLSR PPGHHCLRDK PMGFCLLANI PIAIEAARAK HRVERVAVID WDVHHGNGTQ SIYYDDPNTL TISLHQDRCF PPGYSGADER GAGAGAGSNV NVPLLAGAGD DAYRYAFERI VLPALDAFRP ELVIVASGLD ANAVDPLARM QLHSDSYRYM THALKQAAQR HCGGRLVIVH EGGYSEAYVP FCGHAIVEAL AGMRTDVADP MLELAIAQQP GERFNAFQRQ LIDEMATSF
Validated NT Sequence
ccgaagctcg tcgccatttc gtcgatcagt tgccgctgga atgcgttgaa acgctcgccg ggctgttgcg cgatcgcgag ctcgagcatc ggatcggcga cgtcggtgcg catgcccgcc agtgcctcga cgatcgcatg cccgcaaaac ggtacgtagg cctccgaata accgccctcg tgcacgatga cgagccgtcc cccgcagtgc cgctgcgcgg cctgcttcag cgcatgcgtc atgtaccggt agctgtcgct gtgcagttgc atccgcgcga gcgggtcgac cgcattcgcg tcgagcccgc tcgcgacgat gacgagctcc ggccggaacg catcgagcgc gggcagcacg attcgctcga atgcgtatcg atacgcgtcg tcgccggcgc ccgcgaggag cgggacgttg acgttcgagc ccgcacccgc gcccgcgccg cgttcgtcgg cgccgctgta gccgggcgga aagcagcggt cctgatgcag cgagatcgtc agcgtgttcg gatcgtcgta gtagatcgac tgcgtgccgt tgccgtgatg cacgtcccag tcgatcaccg cgacgcgctc gacgcgatgt ttcgcgcgcg cggcctcgat cgcgatcgga atgttcgcga gcaggcaaaa acccatcggc ttgtcacgca ggcagtgatg gccgggcggg cgcgacagcg agaacgcgtt ggccgtgcgc tcggcgagca ccgcgtcgac ggcggcgagc gcgagccccg cggatagcgc cgcgatctcg tagctgccac ggccgaacgg cgcgagttcg ccgagatcgc cgccacccgc gtcgctcgcg cgcttgaacg cgtcgaggta gtgcgcgggg tggatgcgcc gcagatcgtc gtcggtcgcg gcgggcgcgc tcgacatgtc gagtttcgcc gcgagcccgg acgcgtgcac gagcgacagc aggcgccgct tcgaatcggg cgattccgca tagcccgcgc tcgacggcgg ctgcaccatc gaaccaggac cctgggtctg agct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVQPPSSAG YAESPDSKRR LLSLVHASGL AAKLDMSSAP AATDDDLRRI HPAHYLDAFK RASDAGGGDL GELAPFGRGS YEIAALSAGL ALAAVDAVLA ERTANAFSLS RPPGHHCLRD KPMGFCLLAN IPIAIEAARA KHRVERVAVI DWDVHHGNGT QSIYYDDPNT LTISLHQDRC FPPGYSGADE RGAGAGAGSN VNVPLLAGAG DDAYRYAFER IVLPALDAFR PELVIVASGL DANAVDPLAR MQLHSDSYRY MTHALKQAAQ RHCGGRLVIV HEGGYSEAYV PFCGHAIVEA LAGMRTDVAD PMLELAIAQQ PGERFNAFQR QLIDEMATSF GY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcagccg ccgtcgagcg cgggctatgc ggaatcgccc gattcgaagc ggcgcctgct gtcgctcgtg cacgcgtccg ggctcgcggc gaaactcgac atgtcgagcg cgcccgccgc gaccgacgac gatctgcggc gcatccaccc cgcgcactac ctcgacgcgt tcaagcgcgc gagcgacgcg ggtggcggcg atctcggcga actcgcgccg ttcggccgtg gcagctacga gatcgcggcg ctatccgcgg ggctcgcgct cgccgccgtc gacgcggtgc tcgccgagcg cacggccaac gcgttctcgc tgtcgcgccc gcccggccat cactgcctgc gtgacaagcc gatgggtttt tgcctgctcg cgaacattcc gatcgcgatc gaggccgcgc gcgcgaaaca tcgcgtcgag cgcgtcgcgg tgatcgactg ggacgtgcat cacggcaacg gcacgcagtc gatctactac gacgatccga acacgctgac gatctcgctg catcaggacc gctgctttcc gcccggctac agcggcgccg acgaacgcgg cgcgggcgcg ggtgcgggct cgaacgtcaa cgtcccgctc ctcgcgggcg ccggcgacga cgcgtatcga tacgcattcg agcgaatcgt gctgcccgcg ctcgatgcgt tccggccgga gctcgtcatc gtcgcgagcg ggctcgacgc gaatgcggtc gacccgctcg cgcggatgca actgcacagc gacagctacc ggtacatgac gcatgcgctg aagcaggccg cgcagcggca ctgcggggga cggctcgtca tcgtgcacga gggcggttat tcggaggcct acgtaccgtt ttgcgggcat gcgatcgtcg aggcactggc gggcatgcgc accgacgtcg ccgatccgat gctcgagctc gcgatcgcgc aacagcccgg cgagcgtttc aacgcattcc agcggcaact gatcgacgaa atggcgacga gcttcggtta caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa