BupsA.10154.b
Acetylpolyamine aminohydrolase
| CENTER ID: | BupsA.10154.b |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.10154.b.A1.GE27665 | Full length( BupsA.10154.b ) | 1 | 341 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3MEN
DEPOSITED: 3/31/2010
DETERMINATION: XRay
CLONE: BupsA.10154.b.A1.GE27665
PROTEIN: BupsA.10154.b.A1.PS00412
Publications by SSGCID
SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth J, Christensen JS, Edwards TE, Gardberg AS, Myler PJ, Robinson JI, Staker BL, Stewart LJ
J. Struct. Funct. Genomics - 2011
volume 12, issue 2, pages 83-95
PMID: 21359836; PMCID: PMC3123459
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|320372.6.peg.1332 |
| RefSeq: | YP_332719.1 |
| UniProt: | Q3JUN4 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLTYFHPDQS LHHPRTYFSR GRMRMPQEVP ERAARLVAAA FAMGFPVREP DDFGIAPIAA VHDTHYLRFL ETVHREWKAM PEDWGDEAMS NIFVREPNAL RGVLAQAARH LADGSCPVGE HTWRAAYWSA QSALAAAAAV RDGAPAAYAL CRPPGHHARV DAAGGFCYLN NAAIAAQALR ARHARVAVLD TDMHHGQGIQ EIFYARRDVL YVSIHGDPTN FYPAVAGFDD ERGAGEGLGY NVNLPMPHGS SEAAFFERVD DALRELRRFA PDALVLSLGF DVYRDDPQSQ VAVTTDGFGR LGHLIGALRL PTVIVQEGGY HIESLEANAR SFFGGFGALR G
NT Sequence
ATGCTGACGT ACTTCCACCC CGATCAATCA CTGCATCATC CGCGCACGTA CTTCTCGCGC GGCCGGATGC GCATGCCGCA GGAGGTGCCC GAGCGCGCGG CGCGGCTCGT CGCGGCGGCG TTCGCGATGG GTTTTCCGGT GCGCGAGCCG GACGATTTCG GCATCGCGCC GATCGCGGCC GTGCACGACA CGCACTACCT GCGCTTTCTC GAGACCGTGC ATCGCGAATG GAAGGCGATG CCGGAGGACT GGGGCGACGA AGCGATGTCG AATATTTTCG TGCGCGAGCC GAACGCGTTG CGCGGCGTGC TCGCACAGGC CGCCCGTCAT CTCGCGGACG GCAGTTGCCC GGTCGGCGAG CACACGTGGC GCGCGGCGTA CTGGTCCGCG CAGAGCGCGC TCGCGGCGGC GGCGGCGGTG CGCGACGGCG CGCCCGCAGC GTATGCGCTG TGCCGGCCGC CGGGCCACCA TGCGCGCGTC GACGCCGCGG GCGGCTTCTG TTATCTGAAC AACGCGGCGA TCGCCGCGCA GGCGCTGCGC GCGCGCCATG CGCGCGTCGC CGTCCTCGAC ACCGACATGC ATCACGGGCA AGGCATACAG GAAATCTTCT ACGCGCGGCG CGACGTGCTG TACGTATCGA TTCACGGCGA TCCGACGAAC TTCTACCCGG CCGTCGCGGG CTTCGACGAC GAGCGCGGCG CGGGCGAAGG CCTCGGCTAC AACGTGAATC TGCCGATGCC GCACGGCTCG AGCGAAGCGG CGTTCTTCGA GCGCGTCGAC GATGCGCTGC GCGAGTTGCG GCGCTTCGCG CCCGATGCGC TCGTGCTGTC GCTTGGGTTC GACGTCTATC GCGACGATCC GCAATCGCAG GTGGCGGTGA CGACGGACGG TTTCGGTCGG TTGGGACACC TGATCGGCGC GCTGCGGCTG CCGACCGTCA TCGTGCAGGA AGGCGGCTAT CACATCGAGA GCCTCGAGGC GAATGCGCGG TCGTTCTTCG GCGGATTCGG CGCGCTGCGC GGT
Details for BupsA.10154.b.A1.GE27665
| HARVESTED ON: | 10/13/2009 |
| SEQUENCED ON: | 10/21/2009 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLTYFHPDQ SLHHPRTYFS RGRMRMPQEV PERAARLVAA AFAMGFPVRE PDDFGIAPIA AVHDTHYLRF LETVHREWKA MPEDWGDEAM SNIFVREPNA LRGVLAQAAR HLADGSCPVG EHTWRAAYWS AQSALAAAAA VRDGAPAAYA LCRPPGHHAR VDAAGGFCYL NNAAIAAQAL RARHARVAVL DTDMHHGQGI QEIFYARRDV LYVSIHGDPT NFYPAVAGFD DERGAGEGLG YNVNLPMPHG SSEAAFFERV DDALRELRRF APDALVLSLG FDVYRDDPQS QVAVTTDGFG RLGHLIGALR LPTVIVQEGG YHIESLEANA RSFFGGFGAL RG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctga cgtacttcca ccccgatcaa tcactgcatc atccgcgcac gtacttctcg cgcggccgga tgcgcatgcc gcaggaggtg cccgagcgcg cggcgcggct cgtcgcggcg gcgttcgcga tgggttttcc ggtgcgcgag ccggacgatt tcggcatcgc gccgatcgcg gccgtgcacg acacgcacta cctgcgcttt ctcgagaccg tgcatcgcga atggaaggcg atgccggagg actggggcga cgaagcgatg tcgaatattt tcgtgcgcga gccgaacgcg ttgcgcggcg tgctcgcaca ggccgcccgt catctcgcgg acggcagttg cccggtcggc gagcacacgt ggcgcgcggc gtactggtcc gcgcagagcg cgctcgcggc ggcggcggcg gtgcgcgacg gcgcgcccgc agcgtatgcg ctgtgccggc cgccgggcca ccatgcgcgc gtcgacgccg cgggcggctt ctgttatctg aacaacgcgg cgatcgccgc gcaggcgctg cgcgcgcgcc atgcgcgcgt cgccgtcctc gacaccgaca tgcatcacgg gcaaggcata caggaaatct tctacgcgcg gcgcgacgtg ctgtacgtat cgattcacgg cgatccgacg aacttctacc cggccgtcgc gggcttcgac gacgagcgcg gcgcgggcga aggcctcggc tacaacgtga atctgccgat gccgcacggc tcgagcgaag cggcgttctt cgagcgcgtc gacgatgcgc tgcgcgagtt gcggcgcttc gcgcccgatg cgctcgtgct gtcgcttggg ttcgacgtct atcgcgacga tccgcaatcg caggtggcgg tgacgacgga cggtttcggt cggttgggac acctgatcgg cgcgctgcgg ctgccgaccg tcatcgtgca ggaaggcggc tatcacatcg agagcctcga ggcgaatgcg cggtcgttct tcggcggatt cggcgcgctg cgcggttaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLTYFHPDQ SLHHPRTYFS RGRMRMPQEV PERAARLVAA AFAMGFPVRE PDDFGIAPIA AVHDTHYLRF LETVHREWKA MPEDWGDEAM SNIFVREPNA LRGVLAQAAR HLADGSCPVG EHTWRAAYWS AQSALAAAAA VRDGAPAAYA LCRPPGHHAR VDAAGGFCYL NNAAIAAQAL RARHARVAVL DTDMHHGQGI QEIFYARRDV LYVSIHGDPT NFYPAVAGFD DERGAGEGLG YNVNLPMPHG SSEAAFFERV DDALRELRRF APDALVLSLG FDVYRDDPQS QVAVTTDGFG RLGHLIGALR LPTVIVQEGG YHIESLEANA RSFFGGFGAL RG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgacgtac ttccaccccg atcaatcact gcatcatccg cgcacgtact tctcgcgcgg ccggatgcgc atgccgcagg aggtgcccga gcgcgcggcg cggctcgtcg cggcggcgtt cgcgatgggt tttccggtgc gcgagccgga cgatttcggc atcgcgccga tcgcggccgt gcacgacacg cactacctgc gctttctcga gaccgtgcat cgcgaatgga aggcgatgcc ggaggactgg ggcgacgaag cgatgtcgaa tattttcgtg cgcgagccga acgcgttgcg cggcgtgctc gcacaggccg cccgtcatct cgcggacggc agttgcccgg tcggcgagca cacgtggcgc gcggcgtact ggtccgcgca gagcgcgctc gcggcggcgg cggcggtgcg cgacggcgcg cccgcagcgt atgcgctgtg ccggccgccg ggccaccatg cgcgcgtcga cgccgcgggc ggcttctgtt atctgaacaa cgcggcgatc gccgcgcagg cgctgcgcgc gcgccatgcg cgcgtcgccg tcctcgacac cgacatgcat cacgggcaag gcatacagga aatcttctac gcgcggcgcg acgtgctgta cgtatcgatt cacggcgatc cgacgaactt ctacccggcc gtcgcgggct tcgacgacga gcgcggcgcg ggcgaaggcc tcggctacaa cgtgaatctg ccgatgccgc acggctcgag cgaagcggcg ttcttcgagc gcgtcgacga tgcgctgcgc gagttgcggc gcttcgcgcc cgatgcgctc gtgctgtcgc ttgggttcga cgtctatcgc gacgatccgc aatcgcaggt ggcggtgacg acggacggtt tcggtcggtt gggacacctg atcggcgcgc tgcggctgcc gaccgtcatc gtgcaggaag gcggctatca catcgagagc ctcgaggcga atgcgcggtc gttcttcggc ggattcggcg cgctgcgcgg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa