BupsA.10154.a

histone deacetylase family protein

CENTER ID: BupsA.10154.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10154.a.A1.GE27664 Full length( BupsA.10154.a ) 1 350
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.334
RefSeq: YP_331748.1
UniProt: Q3JXF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYVVPHGTLN LGEHFDSPLR LAYTKQLLDA VGMTERLTRV AFARATDEQL LRVHRPEYLR QLAEACAVAG EQVVRLGDDA AGSASTEDVA RLAAGAACAA VDAVMTGPLR QAYALIRPSG HHAGADFAMG YCYYNNVAIA ARHAQAAHGV ERVAIVDWDV HHGNGTQQAF YDDPSVLFVS LHEAANFPVD GGEARETGGG AGAGYNANVP LPSGTGDAGY RHAFDELVLP LVEAFAPQLI LVSAGQDANA FDPLGRMRVQ RDGFRHMARA LRQAAGGACG GRIVMLQEGG YSLPYLPIAT LGVLEGLVGW NAPFDDPHQF VQYPLGEGER AAVKAARAAL APYWPTLHRS
NT Sequence
GTGTATGTCG TGCCGCACGG CACGCTGAAC CTCGGCGAGC ATTTCGACAG CCCGCTGAGG CTCGCGTATA CGAAGCAGTT GCTCGACGCG GTCGGCATGA CCGAGCGCCT GACGCGCGTC GCTTTCGCGC GTGCGACCGA TGAGCAATTG CTGCGCGTGC ATCGGCCCGA ATATCTGCGG CAGCTCGCCG AGGCTTGCGC GGTGGCGGGC GAGCAGGTGG TGCGGCTCGG CGACGACGCG GCGGGCAGCG CGTCGACCGA AGACGTCGCG CGGCTCGCGG CGGGCGCCGC GTGCGCGGCT GTCGATGCGG TGATGACGGG GCCGCTTCGG CAGGCGTATG CGCTGATCCG GCCGTCTGGC CACCATGCGG GCGCCGATTT CGCGATGGGC TACTGCTACT ACAACAACGT CGCGATCGCG GCGCGCCATG CGCAGGCCGC GCACGGCGTC GAGCGGGTGG CGATCGTCGA CTGGGACGTG CATCACGGCA ACGGCACGCA GCAGGCGTTC TACGACGATC CTTCGGTATT GTTCGTGTCG CTGCACGAGG CGGCGAATTT CCCGGTCGAC GGCGGCGAGG CGCGTGAGAC GGGCGGCGGC GCGGGCGCGG GCTACAACGC GAACGTGCCG TTGCCGTCCG GCACGGGCGA CGCCGGCTAT CGGCACGCGT TCGACGAGCT GGTGCTGCCG CTCGTCGAGG CGTTCGCGCC GCAGCTCATT CTCGTGTCGG CGGGGCAGGA TGCGAACGCG TTCGATCCGC TCGGCCGCAT GCGCGTGCAG CGCGACGGGT TCCGGCACAT GGCGCGCGCA TTGCGGCAAG CGGCGGGCGG CGCATGCGGG GGGCGCATCG TGATGCTGCA GGAGGGCGGC TACAGCCTGC CGTATCTGCC GATCGCGACG CTCGGCGTGC TCGAGGGGCT CGTCGGCTGG AACGCGCCGT TCGACGATCC GCACCAGTTC GTCCAGTATC CGCTCGGCGA AGGCGAGCGC GCCGCGGTGA AGGCCGCCCG CGCCGCGCTG GCGCCGTATT GGCCCACGCT GCATCGATCC
Details for BupsA.10154.a.A1.GE27664
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 22
PERCENT COVERAGE: 95
Validated AA Sequence
MQRGPIRRQR GAGGLHRGAL AFAERILDEL VRIVERRVPA DEPLEHAERR DRQIRQAVAA LLQHHDAPPA CAARRLPQCA RHVPEPVALH AHAAERIERV RILPRRHENE LRRERLDERQ HQLVERVPIA GVARAGRQRH VRVVARARAA ARLTRLAAVD REIRRLVQRH EQYRRIVVER LLRAVAVMHV PVDDRHPLDA VRGLRMARRD RDVVVVAVAH REIGARMVAR RPDQRIRLPK RPRHHRIDSR ARGARREPRD VFGRRAARRV VAEPHHLLAR HRASLGELPQ IFGPMHAQQL LIGRTRESDA RQALGHADRV EQLLRIREPQ RAVEMLAEVQ RAVRHDIHHR TRTLGLS
Validated NT Sequence
atgcagcgtg ggccaatacg gcgccagcgc ggcgcgggcg gccttcaccg cggcgcgctc gccttcgccg agcggatact ggacgaactg gtgcggatcg tcgaacggcg cgttccagcc gacgagcccc tcgagcacgc cgagcgtcgc gatcggcaga tacggcaggc tgtagccgcc ctcctgcagc atcacgatgc gccccccgca tgcgccgccc gccgcttgcc gcaatgcgcg cgccatgtgc cggaacccgt cgcgctgcac gcgcatgcgg ccgagcggat cgaacgcgtt cgcatcctgc cccgccgaca cgagaatgag ctgcggcgcg aacgcctcga cgagcggcag caccagctcg tcgaacgcgt gccgatagcc ggcgtcgccc gtgccggacg gcaacggcac gttcgcgttg tagcccgcgc ccgcgccgcc gcccgtctca cgcgcctcgc cgccgtcgac cgggaaattc gccgcctcgt gcagcgacac gaacaatacc gaaggatcgt cgtagaacgc ctgctgcgtg ccgttgccgt gatgcacgtc ccagtcgacg atcgccaccc gctcgacgcc gtgcgcggcc tgcgcatggc gcgccgcgat cgcgacgttg ttgtagtagc agtagcccat cgcgaaatcg gcgcccgcat ggtggccaga cggccggatc agcgcatacg cctgccgaag cggccccgtc atcaccgcat cgacagccgc gcacgcggcg cccgccgcga gccgcgcgac gtcttcggtc gacgcgctgc ccgccgcgtc gtcgccgagc cgcaccacct gctcgcccgc caccgcgcaa gcctcggcga gctgccgcag atattcgggc cgatgcacgc gcagcaattg ctcatcggtc gcacgcgcga aagcgacgcg cgtcaggcgc tcggtcatgc cgaccgcgtc gagcaactgc ttcgtatacg cgagcctcag cgggctgtcg aaatgctcgc cgaggttcag cgtgccgtgc ggcacgacat acaccatcga accaggaccc tgggtctgag ct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVYVVPHGT LNLGEHFDSP LRLAYTKQLL DAVGMTERLT RVAFARATDE QLLRVHRPEY LRQLAEACAV AGEQVVRLGD DAAGSASTED VARLAAGAAC AAVDAVMTGP LRQAYALIRP SGHHAGADFA MGYCYYNNVA IAARHAQAAH GVERVAIVDW DVHHGNGTQQ AFYDDPSVLF VSLHEAANFP VDGGEARETG GGAGAGYNAN VPLPSGTGDA GYRHAFDELV LPLVEAFAPQ LILVSAGQDA NAFDPLGRMR VQRDGFRHMA RALRQAAGGA CGGRIVMLQE GGYSLPYLPI ATLGVLEGLV GWNAPFDDPH QFVQYPLGEG ERAAVKAARA ALAPYWPTLH RS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtatgtc gtgccgcacg gcacgctgaa cctcggcgag catttcgaca gcccgctgag gctcgcgtat acgaagcagt tgctcgacgc ggtcggcatg accgagcgcc tgacgcgcgt cgctttcgcg cgtgcgaccg atgagcaatt gctgcgcgtg catcggcccg aatatctgcg gcagctcgcc gaggcttgcg cggtggcggg cgagcaggtg gtgcggctcg gcgacgacgc ggcgggcagc gcgtcgaccg aagacgtcgc gcggctcgcg gcgggcgccg cgtgcgcggc tgtcgatgcg gtgatgacgg ggccgcttcg gcaggcgtat gcgctgatcc ggccgtctgg ccaccatgcg ggcgccgatt tcgcgatggg ctactgctac tacaacaacg tcgcgatcgc ggcgcgccat gcgcaggccg cgcacggcgt cgagcgggtg gcgatcgtcg actgggacgt gcatcacggc aacggcacgc agcaggcgtt ctacgacgat ccttcggtat tgttcgtgtc gctgcacgag gcggcgaatt tcccggtcga cggcggcgag gcgcgtgaga cgggcggcgg cgcgggcgcg ggctacaacg cgaacgtgcc gttgccgtcc ggcacgggcg acgccggcta tcggcacgcg ttcgacgagc tggtgctgcc gctcgtcgag gcgttcgcgc cgcagctcat tctcgtgtcg gcggggcagg atgcgaacgc gttcgatccg ctcggccgca tgcgcgtgca gcgcgacggg ttccggcaca tggcgcgcgc attgcggcaa gcggcgggcg gcgcatgcgg ggggcgcatc gtgatgctgc aggagggcgg ctacagcctg ccgtatctgc cgatcgcgac gctcggcgtg ctcgaggggc tcgtcggctg gaacgcgccg ttcgacgatc cgcaccagtt cgtccagtat ccgctcggcg aaggcgagcg cgccgcggtg aaggccgccc gcgccgcgct ggcgccgtat tggcccacgc tgcatcgatc caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa