BupsA.10014.o

putrescine ABC transport system, ATP-binding protein

CENTER ID: BupsA.10014.o
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10014.o.A1.GE27659 Full length( BupsA.10014.o ) 1 384
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.6176
RefSeq: YP_337170.1
UniProt: Q3JGY3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSTPSIDTA QLARPPVGTP AGTNEFVRIE NVVKKFADST AVDNVSLSIA KNELFALLGS SGCGKSTLLR MLAGLETATS GRIFVDGEDL ATLPPYRRPV NMMFQSYALF PHMSVESNVA FGLKQEGTPK HEIRERVADA LNLVQMSRYA RRKPHQLSGG QQQRVALARS LVKRPKLLLL DEPMSALDKK IRQKTQLELV NIIEKVDVTC VMVTHDQEEA MTMASRLAVM SEGRIVQIGT PSQVYEFPNS RFSAEFIGST NLFEGVVVED EPDHIFVESE DLEARMYVSH GVTGPLGMPV GISVRPERVR VSRERPSAPH NWARGVVTDV AYMGSYSLYH VRLPSGKTVV SNLSSSHLMQ DGAPAYNDDV FVSWSPASGV VLTQ
NT Sequence
ATGAAATCGA CGCCTTCAAT CGACACCGCG CAACTGGCGC GTCCCCCGGT CGGCACGCCG GCCGGAACGA ACGAGTTCGT CCGCATCGAG AACGTCGTGA AAAAATTCGC AGACAGCACC GCTGTCGACA ACGTCAGCCT GTCGATCGCG AAAAACGAGC TGTTCGCGCT GCTCGGCAGC TCGGGCTGCG GCAAATCCAC CCTGCTGCGG ATGCTCGCCG GGCTCGAGAC GGCCACGTCC GGGCGCATCT TCGTCGACGG AGAGGATCTC GCGACGCTGC CGCCATATCG CCGGCCCGTG AACATGATGT TCCAGTCGTA CGCGCTCTTT CCGCACATGA GCGTCGAGTC GAACGTCGCG TTCGGACTGA AGCAGGAAGG CACGCCGAAG CACGAGATTC GCGAGCGCGT CGCCGACGCG CTGAACCTCG TGCAGATGAG CCGCTACGCG CGGCGCAAGC CGCATCAGCT CTCGGGCGGC CAGCAGCAGC GCGTCGCGCT CGCGCGCTCG CTCGTCAAGC GGCCGAAACT GCTGCTGCTC GACGAGCCGA TGTCCGCGCT CGACAAGAAG ATCCGCCAGA AGACGCAGCT CGAGCTCGTG AACATCATCG AGAAGGTCGA CGTGACCTGC GTGATGGTCA CGCACGATCA GGAAGAAGCG ATGACGATGG CAAGCCGCCT CGCGGTGATG AGCGAAGGCC GGATCGTGCA GATCGGCACG CCGAGCCAGG TCTACGAATT TCCGAACAGC CGCTTCTCGG CCGAATTCAT CGGCTCGACG AATCTGTTCG AGGGCGTCGT CGTGGAGGAC GAGCCCGATC ACATCTTCGT CGAAAGCGAG GATCTCGAAG CGCGCATGTA CGTGAGTCAC GGCGTCACGG GCCCGCTCGG GATGCCGGTC GGCATCTCGG TTCGCCCGGA GCGCGTGCGC GTGTCGCGCG AGCGGCCGAG CGCACCGCAC AACTGGGCGC GCGGCGTCGT CACCGACGTC GCGTACATGG GCAGCTATTC GCTGTATCAC GTGCGTCTGC CGAGCGGCAA GACGGTCGTA TCCAATCTCT CGAGCTCGCA CCTGATGCAG GACGGTGCGC CCGCGTACAA CGACGACGTG TTCGTCTCCT GGTCGCCCGC AAGCGGCGTG GTGCTGACGC AA
Details for BupsA.10014.o.A1.GE27659
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MGTLEAQTQG PGSMKSTPSI DTAQLARPPV GTPAGTNEFV RIENVVKKFA DSTAVDNVSL SIAKNELFAL LGSSGCGKST LLRMLAGLET ATSGRIFVDG EDLATLPPYR RPVNMMFQSY ALFPHMSVES NVAFGLKQEG TPKHEIRERV ADALNLVQMS RYARRKPHQL SGGQQQRVAL ARSLVKRPKL LLLDEPMSAL DKKIRQKTQL ELVNIIEKVD VTCVMVTHDQ EEAMTMASRL AVMSEGRIVQ IGTPSQVYEF PNSRFSAEFI GSTNLFEGVV VEDEPDHIFV ESEDLEARMY VSHGVTGPLG MPVGISVRPE RVRVSRERPS APHNWARGVV TDVAYMGSYS LYHVRLPSGK TVVSNLSSSH LMQDGAPAYN DDVFVSWSPA
Validated NT Sequence
cttgcgggcg accaggagac gaacacgtcg tcgttgtacg cgggcgcacc gtcctgcatc aggtgcgagc tcgagagatt ggatacgacc gtcttgccgc tcggcagacg cacgtgatac agcgaatagc tgcccatgta cgcgacgtcg gtgacgacgc cgcgcgccca gttgtgcggt gcgctcggcc gctcgcgcga cacgcgcacg cgctccgggc gaaccgagat gccgaccggc atcccgagcg ggcccgtgac gccgtgactc acgtacatgc gcgcttcgag atcctcgctt tcgacgaaga tgtgatcggg ctcgtcctcc acgacgacgc cctcgaacag attcgtcgag ccgatgaatt cggccgagaa gcggctgttc ggaaattcgt agacctggct cggcgtgccg atctgcacga tccggccttc gctcatcacc gcgaggcggc ttgccatcgt catcgcttct tcctgatcgt gcgtgaccat cacgcaggtc acgtcgacct tctcgatgat gttcacgagc tcgagctgcg tcttctggcg gatcttcttg tcgagcgcgg acatcggctc gtcgagcagc agcagtttcg gccgcttgac gagcgagcgc gcgagcgcga cgcgctgctg ctggccgccc gagagctgat gcggcttgcg ccgcgcgtag cggctcatct gcacgaggtt cagcgcgtcg gcgacgcgct cgcgaatctc gtgcttcggc gtgccttcct gcttcagtcc gaacgcgacg ttcgactcga cgctcatgtg cggaaagagc gcgtacgact ggaacatcat gttcacgggc cggcgatatg gcggcagcgt cgcgagatcc tctccgtcga cgaagatgcg cccggacgtg gccgtctcga gcccggcgag catccgcagc agggtggatt tgccgcagcc cgagctgccg agcagcgcga acagctcgtt tttcgcgatc gacaggctga cgttgtcgac agcggtgctg tctgcgaatt ttttcacgac gttctcgatg cggacgaact cgttcgttcc ggccggcgtg ccgaccgggg gacgcgccag ttgcgcggtg tcgattgaag gcgtcgattt catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKSTPSIDT AQLARPPVGT PAGTNEFVRI ENVVKKFADS TAVDNVSLSI AKNELFALLG SSGCGKSTLL RMLAGLETAT SGRIFVDGED LATLPPYRRP VNMMFQSYAL FPHMSVESNV AFGLKQEGTP KHEIRERVAD ALNLVQMSRY ARRKPHQLSG GQQQRVALAR SLVKRPKLLL LDEPMSALDK KIRQKTQLEL VNIIEKVDVT CVMVTHDQEE AMTMASRLAV MSEGRIVQIG TPSQVYEFPN SRFSAEFIGS TNLFEGVVVE DEPDHIFVES EDLEARMYVS HGVTGPLGMP VGISVRPERV RVSRERPSAP HNWARGVVTD VAYMGSYSLY HVRLPSGKTV VSNLSSSHLM QDGAPAYNDD VFVSWSPASG VVLTQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaatcgacg ccttcaatcg acaccgcgca actggcgcgt cccccggtcg gcacgccggc cggaacgaac gagttcgtcc gcatcgagaa cgtcgtgaaa aaattcgcag acagcaccgc tgtcgacaac gtcagcctgt cgatcgcgaa aaacgagctg ttcgcgctgc tcggcagctc gggctgcggc aaatccaccc tgctgcggat gctcgccggg ctcgagacgg ccacgtccgg gcgcatcttc gtcgacggag aggatctcgc gacgctgccg ccatatcgcc ggcccgtgaa catgatgttc cagtcgtacg cgctctttcc gcacatgagc gtcgagtcga acgtcgcgtt cggactgaag caggaaggca cgccgaagca cgagattcgc gagcgcgtcg ccgacgcgct gaacctcgtg cagatgagcc gctacgcgcg gcgcaagccg catcagctct cgggcggcca gcagcagcgc gtcgcgctcg cgcgctcgct cgtcaagcgg ccgaaactgc tgctgctcga cgagccgatg tccgcgctcg acaagaagat ccgccagaag acgcagctcg agctcgtgaa catcatcgag aaggtcgacg tgacctgcgt gatggtcacg cacgatcagg aagaagcgat gacgatggca agccgcctcg cggtgatgag cgaaggccgg atcgtgcaga tcggcacgcc gagccaggtc tacgaatttc cgaacagccg cttctcggcc gaattcatcg gctcgacgaa tctgttcgag ggcgtcgtcg tggaggacga gcccgatcac atcttcgtcg aaagcgagga tctcgaagcg cgcatgtacg tgagtcacgg cgtcacgggc ccgctcggga tgccggtcgg catctcggtt cgcccggagc gcgtgcgcgt gtcgcgcgag cggccgagcg caccgcacaa ctgggcgcgc ggcgtcgtca ccgacgtcgc gtacatgggc agctattcgc tgtatcacgt gcgtctgccg agcggcaaga cggtcgtatc caatctctcg agctcgcacc tgatgcagga cggtgcgccc gcgtacaacg acgacgtgtt cgtctcctgg tcgcccgcaa gcggcgtggt gctgacgcaa aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa