BupsA.10014.n

ABC transporter, ATP-binding protein

CENTER ID: BupsA.10014.n
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10014.n.A1.GE27658 Full length( BupsA.10014.n ) 1 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_132634
PATRIC ID: fig|320372.6.peg.6018
RefSeq: YP_337024.1
UniProt: Q3JHC9
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPHKAAAAPA TRDAAAPAAV PGAAVSFELV GKVFDGPRGP AAALREVTLD IARGGVFGVI GRSGAGKSTL LRLVNGLERP TSGAVRVNGV DVGTLDERGL VALRRRIGMV FQHFNLLSAK TVAQNIGLPL KIAGVPKAER ARKVDALLDL VGLAAKRDAY PASLSGGQKQ RVGIARALVH DPALLLCDEA TSALDPETTQ SILALLADIN RRLGLTIMLI THEMEVIRAV CDTVAVVEQG EVVETGPVWR VFGDPRHGAT RALLRTLAHD LPADLAARLR PLDGAAPLPC GAQLLLDVRY TGASGGEPDL GALTAALARN VGDAVHFVHG GLDRIQGRVQ GRLVIAASLA ARGAASPDRI AAALAAARRH ANRVEVLGYV
NT Sequence
GTGCCGCACA AGGCCGCGGC CGCGCCGGCT ACGCGCGACG CGGCCGCCCC AGCCGCCGTG CCCGGCGCGG CCGTGTCATT CGAGCTCGTC GGCAAGGTGT TCGACGGCCC GCGCGGCCCG GCGGCCGCGC TGCGCGAAGT GACGCTCGAC ATCGCGCGCG GCGGCGTGTT CGGCGTGATC GGGCGCAGCG GCGCGGGCAA GTCGACGCTG CTGCGGCTCG TGAACGGGCT CGAGCGGCCG ACGTCGGGCG CGGTGCGCGT GAACGGCGTC GACGTCGGCA CGCTCGACGA GCGCGGGCTC GTCGCGCTGC GGCGCCGGAT CGGCATGGTG TTTCAGCATT TCAACCTGCT GTCCGCCAAG ACCGTCGCGC AGAACATCGG GCTGCCGTTG AAGATCGCCG GCGTGCCGAA GGCCGAGCGT GCGCGCAAGG TCGACGCGCT GCTCGATCTC GTCGGGCTCG CGGCGAAACG CGACGCGTAT CCGGCGAGCC TGTCGGGCGG CCAGAAGCAG CGCGTCGGCA TCGCGCGCGC GCTCGTCCAC GATCCGGCGC TGCTGTTGTG CGACGAGGCG ACGTCCGCGC TCGATCCGGA GACGACGCAG TCGATTCTCG CGCTGCTCGC CGACATCAAC CGGCGGCTCG GCCTCACGAT CATGCTGATC ACGCACGAGA TGGAAGTGAT CCGCGCGGTG TGCGACACGG TGGCCGTCGT CGAGCAGGGC GAGGTGGTCG AGACGGGGCC GGTGTGGCGC GTGTTCGGCG ATCCGCGGCA CGGCGCGACG CGCGCGCTGT TGCGCACGCT CGCGCACGAC CTGCCGGCCG ATCTCGCGGC GCGCTTGCGG CCGCTCGACG GCGCGGCGCC GCTGCCGTGC GGCGCGCAAC TGCTGCTCGA CGTGCGCTAC ACGGGCGCGA GCGGCGGCGA GCCGGATCTC GGTGCGCTGA CGGCCGCGCT CGCGCGCAAC GTGGGCGACG CGGTGCATTT CGTGCACGGC GGGCTCGATC GGATCCAGGG GCGCGTGCAG GGGCGGCTCG TGATCGCCGC GTCGCTGGCC GCGCGCGGCG CGGCCAGCCC CGACCGGATC GCGGCCGCGC TCGCCGCCGC GCGCCGGCAT GCGAATCGCG TGGAGGTACT CGGCTATGTC
Details for BupsA.10014.n.A1.GE27658
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 78
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPHKAAAA PATRDAAAPA AVPGAAVSFE LVGKVFDGPR GPAAALREVT LDIARGXVFX VIGRSGAGKX TLLXLXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XYPXXLSGGQ XQRVXIARAL VHDPALLLCD EATSALDPET TQSILALLAD INRRLGLTIM LITHEMEVIR AVCDTVAVVE QGEVVETGPV WRVFGDPRHG ATRALLRTLA HDLPADLAAR LRPLDGAAPL PCGAQLLLDV RYTGASGGEP DLGALTAALA RNVGDAVHFV HGGLDRIQGR VQGRLVIAAS LAARGAASPD RIAAALAAAR RHANRVEVLG YV
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPHKAAAA PATRDAAAPA AVPGAAVSFE LVGKVFDGPR GPAAALREVT LDIARGGVFG VIGRSGAGKS TLLRLVNGLE RPTSGAVRVN GVDVGTLDER GLVALRRRIG MVFQHFNLLS AKTVAQNIGL PLKIAGVPKA ERARKVDALL DLVGLAAKRD AYPASLSGGQ KQRVGIARAL VHDPALLLCD EATSALDPET TQSILALLAD INRRLGLTIM LITHEMEVIR AVCDTVAVVE QGEVVETGPV WRVFGDPRHG ATRALLRTLA HDLPADLAAR LRPLDGAAPL PCGAQLLLDV RYTGASGGEP DLGALTAALA RNVGDAVHFV HGGLDRIQGR VQGRLVIAAS LAARGAASPD RIAAALAAAR RHANRVEVLG YV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccgcac aaggccgcgg ccgcgccggc tacgcgcgac gcggccgccc cagccgccgt gcccggcgcg gccgtgtcat tcgagctcgt cggcaaggtg ttcgacggcc cgcgcggccc ggcggccgcg ctgcgcgaag tgacgctcga catcgcgcgc ggcggcgtgt tcggcgtgat cgggcgcagc ggcgcgggca agtcgacgct gctgcggctc gtgaacgggc tcgagcggcc gacgtcgggc gcggtgcgcg tgaacggcgt cgacgtcggc acgctcgacg agcgcgggct cgtcgcgctg cggcgccgga tcggcatggt gtttcagcat ttcaacctgc tgtccgccaa gaccgtcgcg cagaacatcg ggctgccgtt gaagatcgcc ggcgtgccga aggccgagcg tgcgcgcaag gtcgacgcgc tgctcgatct cgtcgggctc gcggcgaaac gcgacgcgta tccggcgagc ctgtcgggcg gccagaagca gcgcgtcggc atcgcgcgcg cgctcgtcca cgatccggcg ctgctgttgt gcgacgaggc gacgtccgcg ctcgatccgg agacgacgca gtcgattctc gcgctgctcg ccgacatcaa ccggcggctc ggcctcacga tcatgctgat cacgcacgag atggaagtga tccgcgcggt gtgcgacacg gtggccgtcg tcgagcaggg cgaggtggtc gagacggggc cggtgtggcg cgtgttcggc gatccgcggc acggcgcgac gcgcgcgctg ttgcgcacgc tcgcgcacga cctgccggcc gatctcgcgg cgcgcttgcg gccgctcgac ggcgcggcgc cgctgccgtg cggcgcgcaa ctgctgctcg acgtgcgcta cacgggcgcg agcggcggcg agccggatct cggtgcgctg acggccgcgc tcgcgcgcaa cgtgggcgac gcggtgcatt tcgtgcacgg cgggctcgat cggatccagg ggcgcgtgca ggggcggctc gtgatcgccg cgtcgctggc cgcgcgcggc gcggccagcc ccgaccggat cgcggccgcg ctcgccgccg cgcgccggca tgcgaatcgc gtggaggtac tcggctatgt caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa