BupsA.10014.k

Maltose/maltodextrin import ATP-binding protein malK

CENTER ID: BupsA.10014.k
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10014.k.A1.GE27655 Full length( BupsA.10014.k ) 1 414
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
RefSeq: WP_004524825.1
RefSeq: YP_336342.1
UniProt: Q3JJB1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASVVLKGVS KAYGGSPPIV RDIDLDITEG EFCVFLGASG CGKSTLLRMI AGLEDVTDGD IYIGSQRMNL IPPARRGVAM VFQSYALFPH MTVYENLSFG LTLSGIAKAT VREKVLNVAH ALQIEHLLDR TPRALSGGQR QRVAIGRAIV REPGVFLFDE PLSNLDAALR VQTRHEIARI HREIGHASTI YVTHDQVEAM TLADRVVLLN SGETARKVGS LAQSGSPLEL YYHPRNRFVA EFIGSPKMNF INAELMDAGE HLAKVRLASG DVLSARVDAR VLDPGASLTL GVRPEDLALG TQTQHFVREV MWQERLGEAT YVYLNGGDAG SHWIAKLPGT TNVSAGQRLP LSVPPDKLYA FDARGNALRR CLDQRTSITT VPMTECAFVD QDDSSMVARM HADGHYITSK RHFR
NT Sequence
ATGGCAAGCG TAGTACTGAA AGGCGTGTCC AAAGCGTACG GAGGCAGTCC GCCCATCGTT CGAGATATCG ATCTCGACAT CACGGAGGGC GAGTTCTGCG TATTTCTTGG CGCATCGGGC TGTGGCAAAT CCACCTTGTT GCGCATGATC GCAGGGCTTG AGGATGTCAC AGATGGAGAC ATCTATATCG GCTCGCAGAG GATGAACCTC ATCCCCCCCG CGCGCCGCGG CGTGGCAATG GTGTTCCAAA GCTACGCGCT CTTTCCTCAC ATGACCGTTT ATGAAAACTT GTCCTTCGGA CTAACGCTGT CAGGGATCGC CAAAGCAACG GTTCGCGAAA AAGTTCTCAA CGTCGCGCAC GCGCTGCAGA TCGAGCACTT GCTGGACCGC ACGCCGCGAG CGCTTTCCGG CGGCCAGCGA CAGCGCGTTG CAATCGGACG CGCCATCGTC CGCGAGCCCG GTGTCTTTCT ATTCGATGAG CCGCTTTCCA ATCTGGACGC AGCCTTGCGC GTTCAGACGC GCCACGAGAT CGCGCGAATT CATCGCGAAA TTGGTCACGC GTCCACTATT TACGTCACCC ACGACCAAGT GGAAGCCATG ACGCTCGCCG ACCGCGTGGT GCTGCTTAAC TCCGGCGAGA CCGCTCGCAA GGTGGGAAGC CTGGCGCAGT CCGGGTCGCC ACTTGAACTC TATTACCATC CGCGCAATCG CTTCGTTGCC GAATTCATCG GTTCCCCCAA GATGAATTTC ATCAACGCCG AACTCATGGA TGCAGGCGAG CATCTGGCCA AAGTGCGACT GGCCAGCGGA GATGTGCTCT CGGCACGTGT CGATGCGAGA GTCCTGGATC CGGGCGCATC GCTCACGCTC GGCGTCCGTC CCGAAGATCT CGCTCTTGGC ACTCAAACCC AACATTTCGT GCGCGAAGTT ATGTGGCAGG AGCGTCTGGG CGAAGCGACA TATGTCTACC TAAATGGTGG TGACGCGGGG TCACATTGGA TCGCCAAACT ACCTGGTACG ACGAACGTCA GCGCCGGCCA GCGCCTTCCG CTTTCCGTGC CTCCAGACAA ACTGTATGCA TTCGACGCCC GCGGCAATGC ACTGCGACGT TGCCTCGATC AGCGAACGTC GATCACTACC GTGCCGATGA CCGAATGCGC CTTCGTCGAT CAAGACGACA GTTCAATGGT CGCTCGAATG CATGCGGATG GACATTACAT CACAAGCAAA CGCCACTTCC GA
Details for BupsA.10014.k.A1.GE27655
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 47kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MGTLEAQTQG PGSMASVVLK GVSKAYGGSP PIVRDIDLDI TEGEFCVFLG ASGCGKSTLL RMIAGLEDVT DGDIYIGSQR MNLIPPARRG VAMVFQSYAL FPHMTVYENL SFGLTLSGIA KATVREKVLN VAHALQIEHL LDRTPRALSG GQRQRVAIGR AIVREPGVFL FDEPLSNLDA ALRVQTRHEI ARIHREIGHA STIYVTHDQV EAMTLADRVV LLNSGETARK VGSLAQSGSP LELYYHPRNR FVAEFIGSPK MNFINAELMD AGEHLAKVRL ASGDVLSARV DARVLDPGAS LTLGVRPEDL ALGTQTQHFV REVMWQERLG EATYVYLNGG DAGSHWIAKL PGTTNVSAGQ RLPLSVPPDK LYAFDARGNA LRRCLDQRTS ITTVPMTECA FVDQDDSSMV ARMHADG
Validated NT Sequence
catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggc aagcgtagta ctgaaaggcg tgtccaaagc gtacggaggc agtccgccca tcgttcgaga tatcgatctc gacatcacgg agggcgagtt ctgcgtattt cttggcgcat cgggctgtgg caaatccacc ttgttgcgca tgatcgcagg gcttgaggat gtcacagatg gagacatcta tatcggctcg cagaggatga acctcatccc ccccgcgcgc cgcggcgtgg caatggtgtt ccaaagctac gcgctctttc ctcacatgac cgtttatgaa aacttgtcct tcggactaac gctgtcaggg atcgccaaag caacggttcg cgaaaaagtt ctcaacgtcg cgcacgcgct gcagatcgag cacttgctgg accgcacgcc gcgagcgctt tccggcggcc agcgacagcg cgttgcaatc ggacgcgcca tcgtccgcga gcccggtgtc tttctattcg atgagccgct ttccaatctg gacgcagcct tgcgcgttca gacgcgccac gagatcgcgc gaattcatcg cgaaattggt cacgcgtcca ctatttacgt cacccacgac caagtggaag ccatgacgct cgccgaccgc gtggtgctgc ttaactccgg cgagaccgct cgcaaggtgg gaagcctggc gcagtccggg tcgccacttg aactctatta ccatccgcgc aatcgcttcg ttgccgaatt catcggttcc cccaagatga atttcatcaa cgccgaactc atggatgcag gcgagcatct ggccaaagtg cgactggcca gcggagatgt gctctcggca cgtgtcgatg cgagagtcct ggatccgggc gcatcgctca cgctcggcgt ccgtcccgaa gatctcgctc ttggcactca aacccaacat ttcgtgcgcg aagttatgtg gcaggagcgt ctgggcgaag cgacatatgt ctacctaaat ggtggtgacg cggggtcaca ttggatcgcc aaactacctg gtacgacgaa cgtcagcgcc ggccagcgcc ttccgctttc cgtgcctcca gacaaactgt atgcattcga cgcccgcggc aatgcactgc gacgttgcct cgatcagcga acgtcgatca ctaccgtgcc gatgaccgaa tgcgccttcg tcgatcaaga cgacagttca atggtcgctc gaatgcatgc ggatggacat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASVVLKGV SKAYGGSPPI VRDIDLDITE GEFCVFLGAS GCGKSTLLRM IAGLEDVTDG DIYIGSQRMN LIPPARRGVA MVFQSYALFP HMTVYENLSF GLTLSGIAKA TVREKVLNVA HALQIEHLLD RTPRALSGGQ RQRVAIGRAI VREPGVFLFD EPLSNLDAAL RVQTRHEIAR IHREIGHAST IYVTHDQVEA MTLADRVVLL NSGETARKVG SLAQSGSPLE LYYHPRNRFV AEFIGSPKMN FINAELMDAG EHLAKVRLAS GDVLSARVDA RVLDPGASLT LGVRPEDLAL GTQTQHFVRE VMWQERLGEA TYVYLNGGDA GSHWIAKLPG TTNVSAGQRL PLSVPPDKLY AFDARGNALR RCLDQRTSIT TVPMTECAFV DQDDSSMVAR MHADGHYITS KRHFR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcaagcgta gtactgaaag gcgtgtccaa agcgtacgga ggcagtccgc ccatcgttcg agatatcgat ctcgacatca cggagggcga gttctgcgta tttcttggcg catcgggctg tggcaaatcc accttgttgc gcatgatcgc agggcttgag gatgtcacag atggagacat ctatatcggc tcgcagagga tgaacctcat cccccccgcg cgccgcggcg tggcaatggt gttccaaagc tacgcgctct ttcctcacat gaccgtttat gaaaacttgt ccttcggact aacgctgtca gggatcgcca aagcaacggt tcgcgaaaaa gttctcaacg tcgcgcacgc gctgcagatc gagcacttgc tggaccgcac gccgcgagcg ctttccggcg gccagcgaca gcgcgttgca atcggacgcg ccatcgtccg cgagcccggt gtctttctat tcgatgagcc gctttccaat ctggacgcag ccttgcgcgt tcagacgcgc cacgagatcg cgcgaattca tcgcgaaatt ggtcacgcgt ccactattta cgtcacccac gaccaagtgg aagccatgac gctcgccgac cgcgtggtgc tgcttaactc cggcgagacc gctcgcaagg tgggaagcct ggcgcagtcc gggtcgccac ttgaactcta ttaccatccg cgcaatcgct tcgttgccga attcatcggt tcccccaaga tgaatttcat caacgccgaa ctcatggatg caggcgagca tctggccaaa gtgcgactgg ccagcggaga tgtgctctcg gcacgtgtcg atgcgagagt cctggatccg ggcgcatcgc tcacgctcgg cgtccgtccc gaagatctcg ctcttggcac tcaaacccaa catttcgtgc gcgaagttat gtggcaggag cgtctgggcg aagcgacata tgtctaccta aatggtggtg acgcggggtc acattggatc gccaaactac ctggtacgac gaacgtcagc gccggccagc gccttccgct ttccgtgcct ccagacaaac tgtatgcatt cgacgcccgc ggcaatgcac tgcgacgttg cctcgatcag cgaacgtcga tcactaccgt gccgatgacc gaatgcgcct tcgtcgatca agacgacagt tcaatggtcg ctcgaatgca tgcggatgga cattacatca caagcaaacg ccacttccga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa