BupsA.10014.j

spermidine/putrescine ABC transporter, ATP-binding protein

CENTER ID: BupsA.10014.j
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10014.j.A1.GE27654 Full length( BupsA.10014.j ) 1 366
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.4855
RefSeq: YP_335975.1
UniProt: Q3JKC8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTDDVIVSF RGVRKTYDGE TLVVKALDLD IRRGEFLTLL GPSGSGKTTC LMMLAGFEFP TGGEIRLDGE LLNNVPPHKR NIGMVFQNYA LFPHLSVEQN VDYPLNVRRM PAAERAARVA VALAMVQMER FAKRYPAQLS GGQQQRIALA RALVFEPKLV LMDEPLGALD KQLREHMQYE LKALHEKLGL TFVYVTHDQG EALTMSDRVA VFDKGIVQQL DTVDRLYESP CNEFVANFIG DSNRLRGTVA SVHGEFCEFR LEDGTRLVGL NAGNAQAGAS GVACIRPERM SVAPGSGRAA NGAANALAGE ARSLIYFGDH VRMRCALPGQ DECFVKVPLG TGALDAFAPG VPISLEFQPE HLRVFA
NT Sequence
ATGAAGACCG ATGATGTGAT CGTCAGCTTT CGCGGCGTGC GCAAGACTTA CGACGGCGAG ACGCTCGTCG TCAAGGCGCT GGATCTGGAT ATCCGGCGCG GCGAATTCCT GACGCTGCTC GGGCCGTCCG GCTCAGGCAA GACCACTTGC CTGATGATGC TCGCGGGCTT CGAATTCCCG ACGGGCGGCG AGATTCGCCT GGACGGCGAA CTGCTGAACA ACGTGCCGCC GCACAAGCGC AACATCGGCA TGGTGTTTCA AAACTATGCG CTCTTTCCCC ATCTGAGCGT CGAGCAGAAC GTCGACTATC CGCTGAACGT GCGGCGGATG CCCGCCGCGG AACGCGCCGC GCGCGTCGCG GTGGCGCTGG CGATGGTGCA GATGGAGCGC TTCGCGAAGC GCTATCCGGC GCAACTCTCG GGCGGGCAGC AGCAGCGCAT CGCGCTCGCG CGCGCACTCG TGTTCGAGCC GAAGCTCGTG CTGATGGACG AGCCGCTCGG CGCGCTCGAC AAGCAGTTGC GCGAACACAT GCAGTACGAA CTGAAGGCGT TGCACGAAAA GCTCGGCCTG ACCTTCGTCT ACGTGACGCA CGATCAGGGC GAGGCGCTGA CGATGTCCGA TCGCGTCGCG GTGTTCGACA AGGGCATCGT GCAACAGCTC GACACCGTCG ATCGGCTGTA CGAATCACCG TGCAACGAGT TCGTCGCGAA TTTCATCGGC GACAGCAATC GGTTACGCGG CACCGTCGCG AGCGTGCACG GCGAGTTTTG CGAATTCCGG CTCGAAGACG GCACGCGCCT CGTCGGCCTG AACGCGGGCA ACGCGCAAGC GGGCGCGTCG GGCGTCGCCT GCATTCGTCC CGAGCGGATG AGCGTCGCGC CGGGCAGCGG CCGCGCGGCG AACGGCGCGG CGAACGCGCT AGCCGGCGAA GCGCGCAGCC TCATCTACTT CGGCGATCAC GTGCGGATGC GCTGCGCGTT GCCGGGGCAG GACGAATGCT TCGTCAAGGT GCCGCTCGGC ACCGGCGCGC TCGATGCGTT CGCGCCCGGC GTGCCGATCT CGCTCGAGTT CCAGCCCGAG CATCTGCGGG TCTTCGCA
Details for BupsA.10014.j.A1.GE27654
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 42kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMKTDDVI VSFRGVRKTY DGETLVVKAL DLDIRRGEFL TLLGPSGSGK TTCLMMLAGF EFPTGGEIRL DGELLNNVPP HKRNIGMVFX NYALFPHLXV EXNVDYPLNV RRMPAAXXAA RVAXAXAMVQ XERFAKRXPA QLSGGQQQRI ALARALVFEP KLVLMDEPLG ALDKQLREHM QYELKALHEK LGLTFVYVTH DQGEALTMSD RVAVFDKGIV QQLDTVDRLY ESPCNEFVAN FIGDSNRLRG TVASVHGEFC EFRLEDGTRL VGLNAGNAQA GASGVACIRP ERMSVAPGSG RAANGAANAL AGEARSLIYF GDHVRMRCAL PGQDECFVKV PLGTGALDAF APGVPISLEF QPEHLRVFA
Validated NT Sequence
atgggtaccc tggaagctca gacccagggt cctggttcga tgaagaccga tgatgtgatc gtcagctttc gcggcgtgcg caagacttac gacggcgaga cgctcgtcgt caaggcgctg gatctggata tccggcgcgg cgaattcctg acgctgctcg ggccgtccgg ctcaggcaag accacttgcc tgatgatgct cgcgggcttc gaattcccga cgggcggcga gattcgcctg gacggcgaac tgctgaacaa cgtgccgccg cacaagcgca acatcggcat ggtgtttnna aactatgcgc tctttcccca tctgancgtc gagcanaacg tcgactatcc gctgaacgtg cggcgnatgc ccgccgcgna acncgccgcg cgcgtcgcgg nggcgcnggc gatggtgcag anggagcgct tcgcgaagcg ctntccggcg caactntcgg gcgggcagca gcagcgcatc gcgctcgcgc gcgcactcgt gttcgagccg aagctcgtgc tgatggacga gccgctcggc gcgctcgaca agcagttgcg cgaacacatg cagtacgaac tgaaggcgtt gcacgaaaag ctcggcctga ccttcgtcta cgtgacgcac gatcagggcg aggcgctgac gatgtccgat cgcgtcgcgg tgttcgacaa gggcatcgtg caacagctcg acaccgtcga tcggctgtac gaatcaccgt gcaacgagtt cgtcgcgaat ttcatcggcg acagcaatcg gttacgcggc accgtcgcga gcgtgcacgg cgagttttgc gaattccggc tcgaagacgg cacgcgcctc gtcggcctga acgcgggcaa cgcgcaagcg ggcgcgtcgg gcgtcgcctg cattcgtccc gagcggatga gcgtcgcgcc gggcagcggc cgcgcggcga acggcgcggc gaacgcgcta gccggcgaag cgcgcagcct catctacttc ggcgatcacg tgcggatgcg ctgcgcgttg ccggggcagg acgaatgctt cgtcaaggtg ccgctcggca ccggcgcgct cgatgcgttc gcgcccggcg tgccgatctc gctcgagttc cagcccgagc atctgcgggt cttcgcataa taa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKTDDVIVS FRGVRKTYDG ETLVVKALDL DIRRGEFLTL LGPSGSGKTT CLMMLAGFEF PTGGEIRLDG ELLNNVPPHK RNIGMVFQNY ALFPHLSVEQ NVDYPLNVRR MPAAERAARV AVALAMVQME RFAKRYPAQL SGGQQQRIAL ARALVFEPKL VLMDEPLGAL DKQLREHMQY ELKALHEKLG LTFVYVTHDQ GEALTMSDRV AVFDKGIVQQ LDTVDRLYES PCNEFVANFI GDSNRLRGTV ASVHGEFCEF RLEDGTRLVG LNAGNAQAGA SGVACIRPER MSVAPGSGRA ANGAANALAG EARSLIYFGD HVRMRCALPG QDECFVKVPL GTGALDAFAP GVPISLEFQP EHLRVFA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagaccgat gatgtgatcg tcagctttcg cggcgtgcgc aagacttacg acggcgagac gctcgtcgtc aaggcgctgg atctggatat ccggcgcggc gaattcctga cgctgctcgg gccgtccggc tcaggcaaga ccacttgcct gatgatgctc gcgggcttcg aattcccgac gggcggcgag attcgcctgg acggcgaact gctgaacaac gtgccgccgc acaagcgcaa catcggcatg gtgtttcaaa actatgcgct ctttccccat ctgagcgtcg agcagaacgt cgactatccg ctgaacgtgc ggcggatgcc cgccgcggaa cgcgccgcgc gcgtcgcggt ggcgctggcg atggtgcaga tggagcgctt cgcgaagcgc tatccggcgc aactctcggg cgggcagcag cagcgcatcg cgctcgcgcg cgcactcgtg ttcgagccga agctcgtgct gatggacgag ccgctcggcg cgctcgacaa gcagttgcgc gaacacatgc agtacgaact gaaggcgttg cacgaaaagc tcggcctgac cttcgtctac gtgacgcacg atcagggcga ggcgctgacg atgtccgatc gcgtcgcggt gttcgacaag ggcatcgtgc aacagctcga caccgtcgat cggctgtacg aatcaccgtg caacgagttc gtcgcgaatt tcatcggcga cagcaatcgg ttacgcggca ccgtcgcgag cgtgcacggc gagttttgcg aattccggct cgaagacggc acgcgcctcg tcggcctgaa cgcgggcaac gcgcaagcgg gcgcgtcggg cgtcgcctgc attcgtcccg agcggatgag cgtcgcgccg ggcagcggcc gcgcggcgaa cggcgcggcg aacgcgctag ccggcgaagc gcgcagcctc atctacttcg gcgatcacgt gcggatgcgc tgcgcgttgc cggggcagga cgaatgcttc gtcaaggtgc cgctcggcac cggcgcgctc gatgcgttcg cgcccggcgt gccgatctcg ctcgagttcc agcccgagca tctgcgggtc ttcgcaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa