BupsA.10014.i

glycine betaine/L-proline transport ATP binding subunit

CENTER ID: BupsA.10014.i
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10014.i.A1.GE27653 Full length( BupsA.10014.i ) 1 389
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.4461
RefSeq: YP_335609.1
UniProt: Q3JLE4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDAPKVVVEG LCKVFGSNPR QALDMLAAGA TKDEVFARTG QVVGVHNVSF DVREGEIFVL MGLSGSGKST LIRLVNRLVE PSAGKVMIDG RDVAAVRRAE LTALRRTDMS MVFQSFALMP QRTVLSNAAF GLEVAGMGRK DRERRAMDVL EQVGLAQFAH KLPAELSGGM QQRVGLARAL AVNPSLMIMD EAFSALDPLK RKEMQNVLLQ LQKEQRRTIM FVSHDLEEAL RIGSRIAIME GGRLVQVGTP QEIIANPADD YVRAFFEGID TSRYLTAGDL MLTGAVPTLS KLDAKHVAAS LNGSAEYAFV LDEARKIRGF VTRDALNGAT PNVRQVESIP RDASLDHVVE RCVAHPHALP VVDDDGCYCG SVDRAVLLKA ITRSRGSHV
NT Sequence
ATGGATGCCC CGAAGGTCGT AGTCGAAGGT CTGTGCAAGG TGTTTGGAAG CAATCCGCGG CAAGCGCTGG ACATGCTCGC CGCAGGCGCG ACGAAGGATG AAGTGTTCGC GCGCACGGGC CAGGTGGTCG GCGTGCACAA CGTGTCGTTC GATGTGCGGG AAGGCGAGAT TTTCGTGCTG ATGGGGCTCT CCGGCTCCGG CAAGTCGACG CTGATCCGGC TCGTCAACCG GCTCGTCGAG CCGAGCGCCG GCAAGGTGAT GATCGACGGG CGCGACGTCG CCGCGGTGCG CCGCGCCGAG CTGACCGCGC TGCGCCGCAC CGACATGAGC ATGGTGTTCC AGTCGTTCGC GCTGATGCCG CAGCGCACGG TGCTGTCGAA CGCCGCGTTC GGCCTCGAAG TGGCCGGCAT GGGCCGCAAG GATCGCGAGC GGCGCGCGAT GGACGTGCTC GAGCAGGTGG GCCTCGCGCA GTTCGCGCAC AAGCTGCCCG CCGAACTCTC GGGCGGCATG CAGCAGCGCG TCGGCCTCGC GCGCGCGCTC GCGGTGAACC CGTCGCTGAT GATCATGGAC GAGGCGTTCT CCGCGCTCGA TCCGTTAAAG CGCAAGGAAA TGCAGAACGT GCTGCTGCAG CTTCAGAAAG AGCAGCGCCG CACGATCATG TTCGTGTCGC ACGATCTCGA GGAGGCGCTG CGCATCGGCA GCCGGATCGC GATCATGGAG GGCGGCCGGC TCGTGCAGGT CGGCACGCCG CAGGAAATCA TCGCGAACCC CGCCGACGAC TACGTGCGCG CGTTCTTCGA AGGCATCGAC ACGAGCCGCT ACCTGACCGC GGGCGACCTG ATGCTCACGG GCGCCGTGCC GACCCTGTCG AAGCTCGATG CGAAGCACGT CGCCGCTTCG CTGAACGGCA GCGCCGAATA CGCGTTCGTG CTCGACGAGG CGCGCAAGAT CCGCGGCTTC GTCACGCGCG ACGCGCTGAA CGGCGCGACG CCGAACGTGC GCCAGGTCGA AAGCATTCCG CGCGACGCAT CGCTCGATCA CGTCGTCGAG CGATGCGTCG CGCATCCGCA CGCGCTGCCC GTCGTCGACG ACGACGGCTG TTACTGCGGC TCGGTCGACC GGGCCGTGCT TCTGAAAGCC ATTACGCGTT CACGAGGTTC CCATGTC
Details for BupsA.10014.i.A1.GE27653
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 44kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDAPKVVVE GLCKVFGSNP RQALDMLAAG ATKDEVFART GQVVGVHNVS FDVREGEIFV LMGLSGSGKS TLIRLVNRLV EPSAGKVMID GRDVAAVRRA ELTALRRTDM SMVFQSFALM PQRTVLSNAA FGLEVAGMGR KDRERRAMDV LEQVGLAQFA HKLPAELSGG MQQRVGLARA LAVNPSLMIM DEAFSALDPL KRKEMQNVLL QLQKEQRRTI MFVSHDLEEA LRIGSRIAIM EGGRLVQVGT PXEIIANPAD DYVRAFFEGI DTSRYLTAGX LMXTGAXXTL XXXRXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXVAHPHAL PVVDDDGCYC GSVDRAVLLK AITRSRGSHV
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDAPKVVVE GLCKVFGSNP RQALDMLAAG ATKDEVFART GQVVGVHNVS FDVREGEIFV LMGLSGSGKS TLIRLVNRLV EPSAGKVMID GRDVAAVRRA ELTALRRTDM SMVFQSFALM PQRTVLSNAA FGLEVAGMGR KDRERRAMDV LEQVGLAQFA HKLPAELSGG MQQRVGLARA LAVNPSLMIM DEAFSALDPL KRKEMQNVLL QLQKEQRRTI MFVSHDLEEA LRIGSRIAIM EGGRLVQVGT PQEIIANPAD DYVRAFFEGI DTSRYLTAGD LMLTGAVPTL SKLDAKHVAA SLNGSAEYAF VLDEARKIRG FVTRDALNGA TPNVRQVESI PRDASLDHVV ERCVAHPHAL PVVDDDGCYC GSVDRAVLLK AITRSRGSHV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatgccccg aaggtcgtag tcgaaggtct gtgcaaggtg tttggaagca atccgcggca agcgctggac atgctcgccg caggcgcgac gaaggatgaa gtgttcgcgc gcacgggcca ggtggtcggc gtgcacaacg tgtcgttcga tgtgcgggaa ggcgagattt tcgtgctgat ggggctctcc ggctccggca agtcgacgct gatccggctc gtcaaccggc tcgtcgagcc gagcgccggc aaggtgatga tcgacgggcg cgacgtcgcc gcggtgcgcc gcgccgagct gaccgcgctg cgccgcaccg acatgagcat ggtgttccag tcgttcgcgc tgatgccgca gcgcacggtg ctgtcgaacg ccgcgttcgg cctcgaagtg gccggcatgg gccgcaagga tcgcgagcgg cgcgcgatgg acgtgctcga gcaggtgggc ctcgcgcagt tcgcgcacaa gctgcccgcc gaactctcgg gcggcatgca gcagcgcgtc ggcctcgcgc gcgcgctcgc ggtgaacccg tcgctgatga tcatggacga ggcgttctcc gcgctcgatc cgttaaagcg caaggaaatg cagaacgtgc tgctgcagct tcagaaagag cagcgccgca cgatcatgtt cgtgtcgcac gatctcgagg aggcgctgcg catcggcagc cggatcgcga tcatggaggg cggccggctc gtgcaggtcg gcacgccgca ggaaatcatc gcgaaccccg ccgacgacta cgtgcgcgcg ttcttcgaag gcatcgacac gagccgctac ctgaccgcgg gcgacctgat gctcacgggc gccgtgccga ccctgtcgaa gctcgatgcg aagcacgtcg ccgcttcgct gaacggcagc gccgaatacg cgttcgtgct cgacgaggcg cgcaagatcc gcggcttcgt cacgcgcgac gcgctgaacg gcgcgacgcc gaacgtgcgc caggtcgaaa gcattccgcg cgacgcatcg ctcgatcacg tcgtcgagcg atgcgtcgcg catccgcacg cgctgcccgt cgtcgacgac gacggctgtt actgcggctc ggtcgaccgg gccgtgcttc tgaaagccat tacgcgttca cgaggttccc atgtcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa