BupsA.10014.g

ABC transporter, ATP-binding component

CENTER ID: BupsA.10014.g
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10014.g.A1.GE27651 Full length( BupsA.10014.g ) 1 379
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.3211
RefSeq: YP_334455.1
UniProt: Q3JPP8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRQRIHSMAS LSIRDVYKTY PNGVPVLKGV DIDIEDGQFL ILVGGSGCGK STLLNMIAGL ETVTKGEIRI GDKVVNDLSP KDRDIAMVFQ SYALYPSMTV RENISFGLNI RKVPKNQQKQ IVDRVAAMLQ IEHLLERKPG QLSGGQRQRV AMGRALARDP ALFLFDEPLS NLDAKLRIEM RAEIKLLHQR LGTTIVYVTH DQIEAMTLGD RIAVMKDGVV QQFGAPQDIY DSPSNLFVAG FIGAPPMNFI NGKLVEQGSG VGIELDTGAM RGVLNLPFDA KRMNGHVGRD VILGLRPERI TDARSAHNGE GARLQPVDVT VDVTEPTGPD THVFAQVNGK RIVSRVHPAA NPQPQQKLSL LFDVSKAVLF DPSTEARIA
NT Sequence
ATGAGACAGA GGATTCACAG CATGGCAAGC CTTTCCATCC GTGACGTGTA CAAGACCTAC CCGAACGGCG TGCCCGTCCT GAAGGGCGTC GACATCGACA TCGAGGACGG TCAGTTCCTG ATTCTCGTCG GCGGCTCGGG CTGCGGGAAG TCGACGCTGC TCAACATGAT CGCGGGCCTC GAGACCGTGA CGAAGGGCGA GATCCGGATC GGCGATAAGG TCGTCAACGA TCTGTCGCCG AAGGATCGCG ACATCGCGAT GGTGTTCCAG TCGTACGCGC TCTATCCGTC GATGACGGTG CGCGAGAACA TCTCGTTCGG GCTGAACATC CGCAAGGTGC CGAAGAACCA GCAGAAGCAG ATCGTCGATC GCGTCGCCGC GATGCTGCAG ATCGAGCACC TGCTCGAGCG CAAGCCGGGG CAGCTCTCGG GCGGCCAGCG GCAGCGCGTC GCGATGGGCC GCGCGCTCGC GCGCGACCCG GCGCTGTTCC TGTTCGACGA GCCGCTGTCG AACCTCGACG CGAAGCTGCG CATCGAGATG CGCGCCGAGA TCAAGCTCTT GCATCAGCGC CTCGGCACGA CGATCGTCTA CGTGACGCAC GACCAGATCG AGGCGATGAC GCTCGGCGAC CGGATCGCGG TGATGAAGGA CGGCGTCGTT CAGCAGTTCG GCGCGCCGCA GGACATCTAC GATTCGCCGT CGAACCTGTT CGTCGCCGGC TTCATCGGCG CGCCGCCGAT GAACTTCATC AACGGCAAGC TCGTCGAGCA GGGCAGCGGC GTGGGCATCG AGCTCGATAC GGGCGCGATG CGCGGCGTGC TGAACCTGCC GTTCGACGCG AAGCGGATGA ACGGCCACGT CGGCCGCGAC GTGATCCTCG GCCTGCGGCC GGAGCGGATC ACCGATGCGC GTAGCGCGCA CAACGGCGAG GGCGCGCGCC TGCAGCCCGT CGACGTGACG GTCGACGTGA CCGAGCCGAC GGGCCCCGAC ACGCACGTGT TCGCCCAGGT CAACGGCAAG CGGATCGTGA GCCGCGTGCA TCCGGCCGCG AACCCGCAGC CGCAGCAGAA GCTGTCGCTG TTGTTCGACG TATCGAAGGC GGTGCTGTTC GATCCGTCGA CGGAGGCGCG GATCGCG
Details for BupsA.10014.g.A1.GE27651
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 43kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMRQRIHS MASLSIRDVY KTYPNGVPVL KGVDIDIEDG QFLILVGGSG CGKSTLLNMI AGLETVTKGE IRIGDKVVND LSPKDRDIAM VFQSYALYPS MTVRENISFG LNIRKVPKNQ QKQIVDRVAA MLQIEHLLER KPGQLSGGQR QRVAMGRALA RDPALFLFDE PLSNLDAKLR IEMRAEIKLL HQRLGTTIVY VTHDQIEAMT LGDRIAVMKD GVVQQFGAPQ DIYDSPSNLF VAGFIGAPPM NFINGKLVEQ GSGVGIELDT GAMRGVLNLP FDAKRMNGHV GRDVILGLRP ERITDARSAH NGEGARLQPV DVTVDVTEPT GPDTHVFAQV NGKRIVSRVH PAANPQPQQK LSLLFDVSKA VLFDPSTE
Validated NT Sequence
cgcctccgtc gacggatcga acagcaccgc cttcgatacg tcgaacaaca gcgacagctt ctgctgcggc tgcgggttcg cggccggatg cacgcggctc acgatccgct tgccgttgac ctgggcgaac acgtgcgtgt cggggcccgt cggctcggtc acgtcgaccg tcacgtcgac gggctgcagg cgcgcgccct cgccgttgtg cgcgctacgc gcatcggtga tccgctccgg ccgcaggccg aggatcacgt cgcggccgac gtggccgttc atccgcttcg cgtcgaacgg caggttcagc acgccgcgca tcgcgcccgt atcgagctcg atgcccacgc cgctgccctg ctcgacgagc ttgccgttga tgaagttcat cggcggcgcg ccgatgaagc cggcgacgaa caggttcgac ggcgaatcgt agatgtcctg cggcgcgccg aactgctgaa cgacgccgtc cttcatcacc gcgatccggt cgccgagcgt catcgcctcg atctggtcgt gcgtcacgta gacgatcgtc gtgccgaggc gctgatgcaa gagcttgatc tcggcgcgca tctcgatgcg cagcttcgcg tcgaggttcg acagcggctc gtcgaacagg aacagcgccg ggtcgcgcgc gagcgcgcgg cccatcgcga cgcgctgccg ctggccgccc gagagctgcc ccggcttgcg ctcgagcagg tgctcgatct gcagcatcgc ggcgacgcga tcgacgatct gcttctgctg gttcttcggc accttgcgga tgttcagccc gaacgagatg ttctcgcgca ccgtcatcga cggatagagc gcgtacgact ggaacaccat cgcgatgtcg cgatccttcg gcgacagatc gttgacgacc ttatcgccga tccggatctc gcccttcgtc acggtctcga ggcccgcgat catgttgagc agcgtcgact tcccgcagcc cgagccgccg acgagaatca ggaactgacc gtcctcgatg tcgatgtcga cgcccttcag gacgggcacg ccgttcgggt aggtcttgta cacgtcacgg atggaaaggc ttgccatgct gtgaatcctc tgtctcatcg aaccaggacc ctgggtctga gcttccaggg tacccatat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRQRIHSMA SLSIRDVYKT YPNGVPVLKG VDIDIEDGQF LILVGGSGCG KSTLLNMIAG LETVTKGEIR IGDKVVNDLS PKDRDIAMVF QSYALYPSMT VRENISFGLN IRKVPKNQQK QIVDRVAAML QIEHLLERKP GQLSGGQRQR VAMGRALARD PALFLFDEPL SNLDAKLRIE MRAEIKLLHQ RLGTTIVYVT HDQIEAMTLG DRIAVMKDGV VQQFGAPQDI YDSPSNLFVA GFIGAPPMNF INGKLVEQGS GVGIELDTGA MRGVLNLPFD AKRMNGHVGR DVILGLRPER ITDARSAHNG EGARLQPVDV TVDVTEPTGP DTHVFAQVNG KRIVSRVHPA ANPQPQQKLS LLFDVSKAVL FDPSTEARIA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagacagagg attcacagca tggcaagcct ttccatccgt gacgtgtaca agacctaccc gaacggcgtg cccgtcctga agggcgtcga catcgacatc gaggacggtc agttcctgat tctcgtcggc ggctcgggct gcgggaagtc gacgctgctc aacatgatcg cgggcctcga gaccgtgacg aagggcgaga tccggatcgg cgataaggtc gtcaacgatc tgtcgccgaa ggatcgcgac atcgcgatgg tgttccagtc gtacgcgctc tatccgtcga tgacggtgcg cgagaacatc tcgttcgggc tgaacatccg caaggtgccg aagaaccagc agaagcagat cgtcgatcgc gtcgccgcga tgctgcagat cgagcacctg ctcgagcgca agccggggca gctctcgggc ggccagcggc agcgcgtcgc gatgggccgc gcgctcgcgc gcgacccggc gctgttcctg ttcgacgagc cgctgtcgaa cctcgacgcg aagctgcgca tcgagatgcg cgccgagatc aagctcttgc atcagcgcct cggcacgacg atcgtctacg tgacgcacga ccagatcgag gcgatgacgc tcggcgaccg gatcgcggtg atgaaggacg gcgtcgttca gcagttcggc gcgccgcagg acatctacga ttcgccgtcg aacctgttcg tcgccggctt catcggcgcg ccgccgatga acttcatcaa cggcaagctc gtcgagcagg gcagcggcgt gggcatcgag ctcgatacgg gcgcgatgcg cggcgtgctg aacctgccgt tcgacgcgaa gcggatgaac ggccacgtcg gccgcgacgt gatcctcggc ctgcggccgg agcggatcac cgatgcgcgt agcgcgcaca acggcgaggg cgcgcgcctg cagcccgtcg acgtgacggt cgacgtgacc gagccgacgg gccccgacac gcacgtgttc gcccaggtca acggcaagcg gatcgtgagc cgcgtgcatc cggccgcgaa cccgcagccg cagcagaagc tgtcgctgtt gttcgacgta tcgaaggcgg tgctgttcga tccgtcgacg gaggcgcgga tcgcgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa