BupsA.10014.d

putrescine ABC transporter, ATP-binding protein

CENTER ID: BupsA.10014.d
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10014.d.A1.GE27648 Full length( BupsA.10014.d ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10014.d.A1.PS00421 Full length( BupsA.10014.d ) 1 386

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.2413
RefSeq: YP_333704.1
UniProt: Q3JRU9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNSQSGAAVA GAPSYRPQTG ADGRAENFVQ IIDVVKKFGE TVAVRNVELS VRKGELFALL GSSGCGKSTL LRMLAGLETI TSGKILIDGE DLAQMPPYKR PVNMMFQSYA LFPHMTVEAN VAFGLKQEGV PKAELKERVR DALELVQMAR YASRKPHQLS GGQQQRVALA RSLVKRPKLL LLDEPMSALD KQIRQRTQIE LVNILDKVGV TCIMVTHDQE EAMTMAGRLA VMSEGQIVQI GTPHEVYEYP NCRFSAEFIG STNLFEGVTV EDEPDHVFIE SPDLPCRLYV NHGITGPLGM PVTVSVRPER IALTRKPADG AYNWGRGVVT NVAYMGGYSL YHVKLDSGKT VIANVSSLAI ADLDSPGWGD EIYVRWSAAA GVVLTS
NT Sequence
ATGAATAGTC AGTCGGGCGC GGCGGTGGCG GGCGCGCCGT CCTATCGGCC GCAGACGGGC GCCGACGGGC GCGCCGAAAA CTTTGTCCAG ATCATCGACG TCGTCAAGAA GTTCGGCGAG ACCGTCGCGG TGCGCAACGT CGAGCTGAGC GTGCGCAAGG GCGAGCTGTT CGCGCTCCTC GGCAGCTCCG GCTGCGGGAA GTCGACGCTG CTGCGCATGC TCGCCGGCCT CGAGACGATC ACGTCGGGCA AGATCCTGAT CGACGGCGAG GATCTCGCGC AGATGCCGCC GTACAAGCGG CCCGTGAACA TGATGTTCCA GTCGTACGCG CTGTTCCCGC ACATGACGGT CGAGGCGAAC GTCGCCTTCG GCCTCAAGCA GGAAGGCGTG CCGAAGGCCG AGCTCAAGGA GCGCGTGCGC GATGCGCTCG AGCTCGTGCA GATGGCGCGC TACGCGAGCC GCAAGCCGCA TCAGCTCTCC GGCGGCCAGC AGCAGCGCGT CGCGCTCGCG CGCTCGCTCG TCAAGCGGCC GAAGCTGCTG CTGCTCGACG AGCCGATGTC GGCGCTCGAC AAGCAGATCC GCCAGCGCAC GCAGATCGAG CTCGTCAACA TCCTCGACAA GGTCGGCGTC ACCTGCATCA TGGTCACGCA CGATCAGGAG GAGGCGATGA CGATGGCGGG GCGTCTCGCC GTGATGAGCG AAGGCCAGAT CGTGCAGATC GGCACGCCGC ACGAAGTCTA CGAATATCCG AACTGCCGCT TCTCCGCCGA GTTCATCGGC TCGACGAACC TGTTCGAGGG CGTGACCGTC GAGGACGAGC CCGATCACGT GTTCATCGAA TCGCCGGACC TGCCGTGCCG GCTGTACGTG AACCACGGGA TCACGGGCCC GCTCGGCATG CCCGTCACGG TGTCGGTGCG CCCCGAGCGC ATCGCGCTGA CCCGCAAGCC CGCCGACGGC GCGTACAACT GGGGGCGCGG CGTCGTCACG AACGTCGCGT ACATGGGCGG CTATTCGCTG TATCACGTGA AGCTCGATTC CGGGAAGACG GTGATCGCGA ACGTGTCGAG CCTCGCGATC GCCGATCTCG ATTCGCCCGG CTGGGGCGAT GAAATCTACG TGCGCTGGAG CGCCGCGGCC GGCGTGGTGC TGACGTCA
Details for BupsA.10014.d.A1.GE27648
HARVESTED ON: 10/13/2009
SEQUENCED ON: 10/21/2009
EXPECTED MW: 44kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNSQSGAAV AGAPSYRPQT GADGRAENFV QIIDVVKKFG ETVAVRNVEL SVRKGELFAL LGSSGCGKST LLRMLAGLET ITSGKILIDG EDLAQMPPYK RPVNMMFQSY ALFPHMTVEA NVAFGLKQEG VPKAELKERV RDALELVQMA RYASRKPHQL SGGQQQRVAL ARSLVKRPKL LLLDEPMSAL DKQIRQRTQI ELVNILDKVG VTCIMVTHDQ EEAMTMAGRL AVMSEGQIVQ IGTPHEVYEY PNCRFSAEFI GSTNLFEGVT VEDEPDHVFI ESPDLPCRLY VNHGITGPLG MPVTVSVRPE RIALTRKPAD GAYNWGRGVV TNVAYMGGYS LYHVKLDSGK TVIANVSSLA IADLDSPGWG DEIYVRWSAA AGVVLTS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaata gtcagtcggg cgcggcggtg gcgggcgcgc cgtcctatcg gccgcagacg ggcgccgacg ggcgcgccga aaactttgtc cagatcatcg acgtcgtcaa gaagttcggc gagaccgtcg cggtgcgcaa cgtcgagctg agcgtgcgca agggcgagct gttcgcgctc ctcggcagct ccggctgcgg gaagtcgacg ctgctgcgca tgctcgccgg cctcgagacg atcacgtcgg gcaagatcct gatcgacggc gaggatctcg cgcagatgcc gccgtacaag cggcccgtga acatgatgtt ccagtcgtac gcgctgttcc cgcacatgac ggtcgaggcg aacgtcgcct tcggcctcaa gcaggaaggc gtgccgaagg ccgagctcaa ggagcgcgtg cgcgatgcgc tcgagctcgt gcagatggcg cgctacgcga gccgcaagcc gcatcagctc tccggcggcc agcagcagcg cgtcgcgctc gcgcgctcgc tcgtcaagcg gccgaagctg ctgctgctcg acgagccgat gtcggcgctc gacaagcaga tccgccagcg cacgcagatc gagctcgtca acatcctcga caaggtcggc gtcacctgca tcatggtcac gcacgatcag gaggaggcga tgacgatggc ggggcgtctc gccgtgatga gcgaaggcca gatcgtgcag atcggcacgc cgcacgaagt ctacgaatat ccgaactgcc gcttctccgc cgagttcatc ggctcgacga acctgttcga gggcgtgacc gtcgaggacg agcccgatca cgtgttcatc gaatcgccgg acctgccgtg ccggctgtac gtgaaccacg ggatcacggg cccgctcggc atgcccgtca cggtgtcggt gcgccccgag cgcatcgcgc tgacccgcaa gcccgccgac ggcgcgtaca actgggggcg cggcgtcgtc acgaacgtcg cgtacatggg cggctattcg ctgtatcacg tgaagctcga ttccgggaag acggtgatcg cgaacgtgtc gagcctcgcg atcgccgatc tcgattcgcc cggctggggc gatgaaatct acgtgcgctg gagcgccgcg gccggcgtgg tgctgacgtc ataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNSQSGAAV AGAPSYRPQT GADGRAENFV QIIDVVKKFG ETVAVRNVEL SVRKGELFAL LGSSGCGKST LLRMLAGLET ITSGKILIDG EDLAQMPPYK RPVNMMFQSY ALFPHMTVEA NVAFGLKQEG VPKAELKERV RDALELVQMA RYASRKPHQL SGGQQQRVAL ARSLVKRPKL LLLDEPMSAL DKQIRQRTQI ELVNILDKVG VTCIMVTHDQ EEAMTMAGRL AVMSEGQIVQ IGTPHEVYEY PNCRFSAEFI GSTNLFEGVT VEDEPDHVFI ESPDLPCRLY VNHGITGPLG MPVTVSVRPE RIALTRKPAD GAYNWGRGVV TNVAYMGGYS LYHVKLDSGK TVIANVSSLA IADLDSPGWG DEIYVRWSAA AGVVLTS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatagtcag tcgggcgcgg cggtggcggg cgcgccgtcc tatcggccgc agacgggcgc cgacgggcgc gccgaaaact ttgtccagat catcgacgtc gtcaagaagt tcggcgagac cgtcgcggtg cgcaacgtcg agctgagcgt gcgcaagggc gagctgttcg cgctcctcgg cagctccggc tgcgggaagt cgacgctgct gcgcatgctc gccggcctcg agacgatcac gtcgggcaag atcctgatcg acggcgagga tctcgcgcag atgccgccgt acaagcggcc cgtgaacatg atgttccagt cgtacgcgct gttcccgcac atgacggtcg aggcgaacgt cgccttcggc ctcaagcagg aaggcgtgcc gaaggccgag ctcaaggagc gcgtgcgcga tgcgctcgag ctcgtgcaga tggcgcgcta cgcgagccgc aagccgcatc agctctccgg cggccagcag cagcgcgtcg cgctcgcgcg ctcgctcgtc aagcggccga agctgctgct gctcgacgag ccgatgtcgg cgctcgacaa gcagatccgc cagcgcacgc agatcgagct cgtcaacatc ctcgacaagg tcggcgtcac ctgcatcatg gtcacgcacg atcaggagga ggcgatgacg atggcggggc gtctcgccgt gatgagcgaa ggccagatcg tgcagatcgg cacgccgcac gaagtctacg aatatccgaa ctgccgcttc tccgccgagt tcatcggctc gacgaacctg ttcgagggcg tgaccgtcga ggacgagccc gatcacgtgt tcatcgaatc gccggacctg ccgtgccggc tgtacgtgaa ccacgggatc acgggcccgc tcggcatgcc cgtcacggtg tcggtgcgcc ccgagcgcat cgcgctgacc cgcaagcccg ccgacggcgc gtacaactgg gggcgcggcg tcgtcacgaa cgtcgcgtac atgggcggct attcgctgta tcacgtgaag ctcgattccg ggaagacggt gatcgcgaac gtgtcgagcc tcgcgatcgc cgatctcgat tcgcccggct ggggcgatga aatctacgtg cgctggagcg ccgcggccgg cgtggtgctg acgtcaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BupsA.10014.d.A1.PS00421
PURIFICATION DATe: 11/17/2009
CONCENTRATION: 14.38mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNSQSGAAV AGAPSYRPQT GADGRAENFV QIIDVVKKFG ETVAVRNVEL SVRKGELFAL LGSSGCGKST LLRMLAGLET ITSGKILIDG EDLAQMPPYK RPVNMMFQSY ALFPHMTVEA NVAFGLKQEG VPKAELKERV RDALELVQMA RYASRKPHQL SGGQQQRVAL ARSLVKRPKL LLLDEPMSAL DKQIRQRTQI ELVNILDKVG VTCIMVTHDQ EEAMTMAGRL AVMSEGQIVQ IGTPHEVYEY PNCRFSAEFI GSTNLFEGVT VEDEPDHVFI ESPDLPCRLY VNHGITGPLG MPVTVSVRPE RIALTRKPAD GAYNWGRGVV TNVAYMGGYS LYHVKLDSGK TVIANVSSLA IADLDSPGWG DEIYVRWSAA AGVVLTS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaata gtcagtcggg cgcggcggtg gcgggcgcgc cgtcctatcg gccgcagacg ggcgccgacg ggcgcgccga aaactttgtc cagatcatcg acgtcgtcaa gaagttcggc gagaccgtcg cggtgcgcaa cgtcgagctg agcgtgcgca agggcgagct gttcgcgctc ctcggcagct ccggctgcgg gaagtcgacg ctgctgcgca tgctcgccgg cctcgagacg atcacgtcgg gcaagatcct gatcgacggc gaggatctcg cgcagatgcc gccgtacaag cggcccgtga acatgatgtt ccagtcgtac gcgctgttcc cgcacatgac ggtcgaggcg aacgtcgcct tcggcctcaa gcaggaaggc gtgccgaagg ccgagctcaa ggagcgcgtg cgcgatgcgc tcgagctcgt gcagatggcg cgctacgcga gccgcaagcc gcatcagctc tccggcggcc agcagcagcg cgtcgcgctc gcgcgctcgc tcgtcaagcg gccgaagctg ctgctgctcg acgagccgat gtcggcgctc gacaagcaga tccgccagcg cacgcagatc gagctcgtca acatcctcga caaggtcggc gtcacctgca tcatggtcac gcacgatcag gaggaggcga tgacgatggc ggggcgtctc gccgtgatga gcgaaggcca gatcgtgcag atcggcacgc cgcacgaagt ctacgaatat ccgaactgcc gcttctccgc cgagttcatc ggctcgacga acctgttcga gggcgtgacc gtcgaggacg agcccgatca cgtgttcatc gaatcgccgg acctgccgtg ccggctgtac gtgaaccacg ggatcacggg cccgctcggc atgcccgtca cggtgtcggt gcgccccgag cgcatcgcgc tgacccgcaa gcccgccgac ggcgcgtaca actgggggcg cggcgtcgtc acgaacgtcg cgtacatggg cggctattcg ctgtatcacg tgaagctcga ttccgggaag acggtgatcg cgaacgtgtc gagcctcgcg atcgccgatc tcgattcgcc cggctggggc gatgaaatct acgtgcgctg gagcgccgcg gccggcgtgg tgctgacgtc ataataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNSQSGAAV AGAPSYRPQT GADGRAENFV QIIDVVKKFG ETVAVRNVEL SVRKGELFAL LGSSGCGKST LLRMLAGLET ITSGKILIDG EDLAQMPPYK RPVNMMFQSY ALFPHMTVEA NVAFGLKQEG VPKAELKERV RDALELVQMA RYASRKPHQL SGGQQQRVAL ARSLVKRPKL LLLDEPMSAL DKQIRQRTQI ELVNILDKVG VTCIMVTHDQ EEAMTMAGRL AVMSEGQIVQ IGTPHEVYEY PNCRFSAEFI GSTNLFEGVT VEDEPDHVFI ESPDLPCRLY VNHGITGPLG MPVTVSVRPE RIALTRKPAD GAYNWGRGVV TNVAYMGGYS LYHVKLDSGK TVIANVSSLA IADLDSPGWG DEIYVRWSAA AGVVLTS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATAGTCAG TCGGGCGCGG CGGTGGCGGG CGCGCCGTCC TATCGGCCGC AGACGGGCGC CGACGGGCGC GCCGAAAACT TTGTCCAGAT CATCGACGTC GTCAAGAAGT TCGGCGAGAC CGTCGCGGTG CGCAACGTCG AGCTGAGCGT GCGCAAGGGC GAGCTGTTCG CGCTCCTCGG CAGCTCCGGC TGCGGGAAGT CGACGCTGCT GCGCATGCTC GCCGGCCTCG AGACGATCAC GTCGGGCAAG ATCCTGATCG ACGGCGAGGA TCTCGCGCAG ATGCCGCCGT ACAAGCGGCC CGTGAACATG ATGTTCCAGT CGTACGCGCT GTTCCCGCAC ATGACGGTCG AGGCGAACGT CGCCTTCGGC CTCAAGCAGG AAGGCGTGCC GAAGGCCGAG CTCAAGGAGC GCGTGCGCGA TGCGCTCGAG CTCGTGCAGA TGGCGCGCTA CGCGAGCCGC AAGCCGCATC AGCTCTCCGG CGGCCAGCAG CAGCGCGTCG CGCTCGCGCG CTCGCTCGTC AAGCGGCCGA AGCTGCTGCT GCTCGACGAG CCGATGTCGG CGCTCGACAA GCAGATCCGC CAGCGCACGC AGATCGAGCT CGTCAACATC CTCGACAAGG TCGGCGTCAC CTGCATCATG GTCACGCACG ATCAGGAGGA GGCGATGACG ATGGCGGGGC GTCTCGCCGT GATGAGCGAA GGCCAGATCG TGCAGATCGG CACGCCGCAC GAAGTCTACG AATATCCGAA CTGCCGCTTC TCCGCCGAGT TCATCGGCTC GACGAACCTG TTCGAGGGCG TGACCGTCGA GGACGAGCCC GATCACGTGT TCATCGAATC GCCGGACCTG CCGTGCCGGC TGTACGTGAA CCACGGGATC ACGGGCCCGC TCGGCATGCC CGTCACGGTG TCGGTGCGCC CCGAGCGCAT CGCGCTGACC CGCAAGCCCG CCGACGGCGC GTACAACTGG GGGCGCGGCG TCGTCACGAA CGTCGCGTAC ATGGGCGGCT ATTCGCTGTA TCACGTGAAG CTCGATTCCG GGAAGACGGT GATCGCGAAC GTGTCGAGCC TCGCGATCGC CGATCTCGAT TCGCCCGGCT GGGGCGATGA AATCTACGTG CGCTGGAGCG CCGCGGCCGG CGTGGTGCTG ACGTCAAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA