BupsA.10014.a

sulfate ABC transporter, ATP-binding protein

CENTER ID: BupsA.10014.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.10014.a.A1.GE28455 Full length( BupsA.10014.a ) 1 351
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|320372.6.peg.2095
RefSeq: YP_333412.1
UniProt: Q3JSP1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGITVRNLHK RFGEFAALDD VSLDFPAGEL VALLGPSGCG KTTLLRVIAG LEHADSGQVV LQGLDVASVG ARERQVGFVF QHYALFRHMT VFENVAFGLR VKPRRERPSE AAIRAKVHEL LSLVQLDWLA QRYPSELSGG QRQRIALARA LAVEPKVLLL DEPFGALDAK VRKELRGWLR RLHDDLHIST IFVTHDQEEA LEVADRIVVL NHGRVEQVGS PQAVYDHPRS AFVYEFLGAA NRLDGTVSGN GFVAHGAAQA IAVDADFAGP ARAYVRPHDL ELAAPHARAQ GIAADVRRVV PLGGSVRVEL AARSGEVLEA ELDRNAWRAL ALDVGDALTA VPRAVRVFPA R
NT Sequence
ATGGGCATCA CCGTTCGTAA TCTGCACAAA CGCTTCGGCG AGTTCGCCGC GCTCGACGAC GTCTCGCTCG ATTTCCCCGC GGGTGAGCTC GTCGCGCTGC TCGGCCCGTC CGGCTGCGGC AAGACGACGC TGTTGCGCGT GATCGCGGGC CTCGAGCACG CGGATTCGGG GCAGGTCGTG CTGCAAGGGC TCGACGTCGC GTCGGTCGGC GCGCGCGAGC GGCAGGTCGG CTTCGTGTTC CAGCACTATG CGCTGTTCCG CCACATGACG GTGTTCGAGA ACGTCGCGTT CGGGCTGCGC GTGAAGCCGC GCCGCGAGCG GCCGAGCGAG GCGGCGATCC GCGCGAAGGT GCACGAGCTG CTCTCGCTCG TGCAGCTCGA CTGGCTCGCG CAGCGCTATC CGTCCGAGCT GTCGGGCGGG CAGCGGCAGC GGATCGCGCT CGCGCGCGCG CTCGCCGTCG AGCCGAAAGT GCTGCTGCTC GACGAGCCGT TCGGCGCGCT CGACGCGAAG GTGCGCAAGG AGCTGCGCGG CTGGCTGCGC CGGCTGCACG ACGATCTGCA CATCTCGACG ATCTTCGTCA CGCACGATCA GGAGGAGGCG CTCGAGGTGG CCGACCGGAT CGTCGTGCTC AATCACGGCC GCGTCGAGCA GGTCGGCAGC CCGCAGGCGG TCTACGATCA TCCGCGCAGC GCGTTCGTCT ACGAGTTCCT CGGCGCGGCG AACCGGCTCG ACGGCACGGT GAGCGGCAAC GGTTTCGTCG CGCACGGCGC GGCGCAGGCG ATCGCCGTCG ACGCGGATTT CGCGGGCCCG GCGCGCGCGT ATGTGCGCCC GCACGACCTG GAGCTCGCCG CGCCGCACGC GCGCGCGCAA GGCATCGCGG CCGACGTGCG GCGCGTCGTG CCGCTCGGCG GCTCGGTGCG GGTGGAGCTC GCCGCGCGCT CCGGCGAGGT GCTCGAGGCG GAGCTCGATC GCAACGCATG GCGCGCGCTC GCGCTCGACG TCGGCGACGC GCTGACCGCC GTGCCGCGCG CGGTGCGCGT GTTCCCGGCG CGC
Details for BupsA.10014.a.A1.GE28455
HARVESTED ON: 2/2/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 40kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMGITVRN LHKRFGEFAA LDDVSLDFPA GELVALLGPS GCGKTTLLRV IAGLEHADSG QVVLQGLDVA SVGARERQVG FVFQHYALFR HMTVFENVAF GLRVKPRRER PSEAAIRAKV HELLSLVQLD WLAQRYPSEL SGGQRQRIAL ARALAVEPKV LLLDEPFGAL DAKVRKELRG WLRRLHDDLH ISTIFVTHDQ EEALEVADRI VVLNHGRVEQ VGSPQAVYDH PRSAFVYEFL GAANRLDGTV SGNGFVAHGA AQAIAVDADF AGPARAYVRP HDLELAAPHA RAQGIAADVR RVVPLGGSVR VELAARSGEV LEAELDRNAW RALALDVGDA LTAVPRAVRV FPAR
Validated NT Sequence
ttattagcgc gccgggaaca cgcgcaccgc gcgcggcacg gcggtcagcg cgtcgccgac gtcgagcgcg agcgcgcgcc atgcgttgcg atcgagctcc gcctcgagca cctcgccgga gcgcgcggcg agctccaccc gcaccgagcc gccgagcggc acgacgcgcc gcacgtcggc cgcgatgcct tgcgcgcgcg cgtgcggcgc ggcgagctcc aggtcgtgcg ggcgcacata cgcgcgcgcc gggcccgcga aatccgcgtc gacggcgatc gcctgcgccg cgccgtgcgc gacgaaaccg ttgccgctca ccgtgccgtc gagccggttc gccgcgccga ggaactcgta gacgaacgcg ctgcgcggat gatcgtagac cgcctgcggg ctgccgacct gctcgacgcg gccgtgattg agcacgacga tccggtcggc cacctcgagc gcctcctcct gatcgtgcgt gacgaagatc gtcgagatgt gcagatcgtc gtgcagccgg cgcagccagc cgcgcagctc cttgcgcacc ttcgcgtcga gcgcgccgaa cggctcgtcg agcagcagca ctttcggctc gacggcgagc gcgcgcgcga gcgcgatccg ctgccgctgc ccgcccgaca gctcggacgg atagcgctgc gcgagccagt cgagctgcac gagcgagagc agctcgtgca ccttcgcgcg gatcgccgcc tcgctcggcc gctcgcggcg cggcttcacg cgcagcccga acgcgacgtt ctcgaacacc gtcatgtggc ggaacagcgc atagtgctgg aacacgaagc cgacctgccg ctcgcgcgcg ccgaccgacg cgacgtcgag cccttgcagc acgacctgcc ccgaatccgc gtgctcgagg cccgcgatca cgcgcaacag cgtcgtcttg ccgcagccgg acgggccgag cagcgcgacg agctcacccg cggggaaatc gagcgagacg tcgtcgagcg cggcgaactc gccgaagcgt ttgtgcagat tacgaacggt gatgcccatc gaaccaggac cctgggtctg agcttccagg gtacccatat ga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGITVRNLH KRFGEFAALD DVSLDFPAGE LVALLGPSGC GKTTLLRVIA GLEHADSGQV VLQGLDVASV GARERQVGFV FQHYALFRHM TVFENVAFGL RVKPRRERPS EAAIRAKVHE LLSLVQLDWL AQRYPSELSG GQRQRIALAR ALAVEPKVLL LDEPFGALDA KVRKELRGWL RRLHDDLHIS TIFVTHDQEE ALEVADRIVV LNHGRVEQVG SPQAVYDHPR SAFVYEFLGA ANRLDGTVSG NGFVAHGAAQ AIAVDADFAG PARAYVRPHD LELAAPHARA QGIAADVRRV VPLGGSVRVE LAARSGEVLE AELDRNAWRA LALDVGDALT AVPRAVRVFP AR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcatcacc gttcgtaatc tgcacaaacg cttcggcgag ttcgccgcgc tcgacgacgt ctcgctcgat ttccccgcgg gtgagctcgt cgcgctgctc ggcccgtccg gctgcggcaa gacgacgctg ttgcgcgtga tcgcgggcct cgagcacgcg gattcggggc aggtcgtgct gcaagggctc gacgtcgcgt cggtcggcgc gcgcgagcgg caggtcggct tcgtgttcca gcactatgcg ctgttccgcc acatgacggt gttcgagaac gtcgcgttcg ggctgcgcgt gaagccgcgc cgcgagcggc cgagcgaggc ggcgatccgc gcgaaggtgc acgagctgct ctcgctcgtg cagctcgact ggctcgcgca gcgctatccg tccgagctgt cgggcgggca gcggcagcgg atcgcgctcg cgcgcgcgct cgccgtcgag ccgaaagtgc tgctgctcga cgagccgttc ggcgcgctcg acgcgaaggt gcgcaaggag ctgcgcggct ggctgcgccg gctgcacgac gatctgcaca tctcgacgat cttcgtcacg cacgatcagg aggaggcgct cgaggtggcc gaccggatcg tcgtgctcaa tcacggccgc gtcgagcagg tcggcagccc gcaggcggtc tacgatcatc cgcgcagcgc gttcgtctac gagttcctcg gcgcggcgaa ccggctcgac ggcacggtga gcggcaacgg tttcgtcgcg cacggcgcgg cgcaggcgat cgccgtcgac gcggatttcg cgggcccggc gcgcgcgtat gtgcgcccgc acgacctgga gctcgccgcg ccgcacgcgc gcgcgcaagg catcgcggcc gacgtgcggc gcgtcgtgcc gctcggcggc tcggtgcggg tggagctcgc cgcgcgctcc ggcgaggtgc tcgaggcgga gctcgatcgc aacgcatggc gcgcgctcgc gctcgacgtc ggcgacgcgc tgaccgccgt gccgcgcgcg gtgcgcgtgt tcccggcgcg caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa