BupsA.01674.a

BopA protein

CENTER ID:	BupsA.01674.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.01674.a.A1.GE26287	BupsA.01674.a		1	512

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.01674.a.A1.PS01851	BupsA.01674.a		1	512

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_193182
PATRIC ID:	fig\|320372.6.peg.4580
RefSeq:	YP_335721.1
UniProt:	Q3JL32

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MINVDAFVAS ARSGARVVVG GDARGPVVSA ARLGMKERLF AFLAHVPLLK HCDAVRRYAE QVRMENRRSL EVFVLALSKR YGPEGAKAAF DYGARRDGAP LDQRRVRNMV SIAEHFHGTG DAKPLARQMV FRSWECRGLD HPGHASLTIK NQADADAGRH VYEHVSWWPN QRLGSKEHFD RIEPKTLDGY RIDKRSEISS ATEQRLREGD AARRKILADG FKYANQDERH DARFFPRAGQ KLDKDAEWGL SARKVYFPAI GFNHDRRDTD RPRAFVLFGL NEAAMLRDAR TVKEGAKSGE LMYQMISKKE NCASMALRVL RAGGAEHFVP YTAAWISEDP NHAHAYALAV QARIDALNQR RADVERRCER LRDSASVRQA WRAFSEAGGA SASPLAEDAG RGRASAHMRQ ARLDEHAREV ERIGAYFAEL SAGRSGKHRD RADAALADAM KRCAPSARDD VAALTRKASV LVETLGRHLD APPPSDSSAL RRLAAHAMIG RIEAFMAAAI AA

NT Sequence

atgatcaatg tcgatgcatt cgtcgcaagc gcgcgatccg gcgcgcgtgt cgtcgtcggc ggcgacgcgc gcggcccggt ggtgtcggcc gcgaggttgg ggatgaagga gcggttgttc gcgtttctcg cgcacgtgcc gctgctcaag cactgcgatg cggtgcggcg ctatgcggag caggttcgca tggaaaaccg ccgctcgctc gaggtgttcg tgctcgcgct gtcgaagcga tatggcccgg aaggcgcgaa ggcggcgttc gactacggcg cgcggcgcga cggcgcgccg ctcgatcagc ggcgcgtgcg caacatggtg tcgatcgccg agcacttcca cggcacgggc gacgcgaagc cgctcgcccg gcaaatggta tttcggtcgt gggaatgccg ggggctcgat catccggggc acgcgtcgct gacgatcaag aatcaggcgg acgccgatgc cggccggcac gtgtacgagc acgtcagttg gtggccgaat caacgactgg gtagtaagga gcatttcgat cgcatcgaac cgaagacgct ggacggctat cggatcgaca agcgctcgga gatttcgagc gcgacggaac agaggcttcg cgaaggcgac gcggcgagga gaaagatcct cgcggacggc ttcaaatacg cgaatcagga cgagcgtcac gacgcgcggt tttttccgag agccggccag aagttggaca aggatgccga atgggggctg agcgcgcgca aggtctattt tccggcgatc ggattcaacc acgacaggcg cgatacggac cgcccgcgcg cgttcgtgct gttcgggctg aacgaggcgg cgatgttgcg cgatgcgcgc accgtgaagg aaggcgcgaa gtcaggcgag ctcatgtatc aaatgatcag caagaaggaa aactgcgcat cgatggcgct gcgcgtgctg cgcgccggcg gcgccgagca tttcgtgccg tatacggccg catggatctc cgaggatccg aatcacgcgc acgcctatgc gctggccgtg caggcgagga tcgacgcgct gaaccagcgg cgtgccgacg tcgagcgccg ctgcgagcgg ttgcgcgaca gcgcatcggt gcggcaggcg tggcgcgcgt tctccgaggc cggcggtgcc tcggcaagcc cgctcgcgga ggacgccggc cgcgggcgag catcggcgca catgcggcag gcgcggctcg acgagcatgc gcgcgaggtc gagcgaatcg gcgcgtattt cgccgagctg tccgccgggc gatccggcaa gcatcgcgat cgggcggatg cggctctggc cgatgcgatg aagcgttgcg cgccttcggc tcgcgacgac gtcgcggcgc tgacgcgcaa ggcgagcgtg ctcgtcgaga cgctcggccg gcatctcgac gcgccgccgc cgagcgattc gtccgcgctg aggaggctgg ccgcgcacgc gatgatcggc cggatcgagg cgttcatggc ggctgcgatc gccgcctga

Details for BupsA.01674.a.A1.GE26287

HARVESTED ON:	3/10/2009
SEQUENCED ON:	3/26/2009
EXPECTED MW:	59kDa
OBSERVED MW:	62kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	57
PERCENT COVERAGE:	96

Validated AA Sequence

MINVDAFVAS ARSGARVVVG GDARGPVVSA ARLGMKERLF AFLAHVPLLK HCDAVRRYAE QVRMENRRSL EVFVLALSKR YGPEGAKAAF DYGARRDGAP LDQRRVRNMV SIAEHFHGTG DAKPLARXXX FRSWECRGLD HPGHASLTIK XQADADAGRH VYEHVSWWXN XRLXSKEHFD RIEPXXLDGY RIDXRSXISS AXXQRLREXD AARRKILADG FKYXNQDERH DARFFREPAR SWXRMPNGXE RAQGLFSGDR IQPRQARYGP PARVRAVRAE RGGDVARCAH REGRREVRRA HVSNDQQEGK LRIDXAARAA RRRRRAFRAV YGRMDLRGSE SRARLCAGRA GEDRRAEPAA CRRRAPLRAV ARQRIGAAGV ARVLRGRRCL GKPARGGRRP RASIGAHAAG AARRACARGR ANRRVFRRAV RRAIRQASRS GGCGSGRCDE ALRAFGSRRR RGADAQGERA RRDARPASRR AAAERFVRAE EAGRARDDRP DRGVHGGCDR RLI

Validated NT Sequence

ttattaggcg gcgatcgcag ccgccatgaa cgcctcgatc cggccgatca tcgcgtgcgc ggccagcctc ctcagcgcgg acgaatcgct cggcggcggc gcgtcgagat gccggccgag cgtctcgacg agcacgctcg ccttgcgcgt cagcgccgcg acgtcgtcgc gagccgaagg cgcgcaacgc ttcatcgcat cggccagagc cgcatccgcc cgatcgcgat gcttgccgga tcgcccggcg gacagctcgg cgaaatacgc gccgattcgc tcgacctcgc gcgcatgctc gtcgagccgc gcctgccgca tgtgcgccga tgctcgcccg cggccggcgt cctccgcgag cgggcttgcc gaggcaccgc cggcctcgga gaacgcgcgc cacgcctgcc gcaccgatgc gctgtcgcgc aaccgctcgc agcggcgctc gacgtcggca cgccgctggt tcagcgcgtc gatcctcgcc tgcacggcca gcgcataggc gtgcgcgtga ttcggatcct cggagatcca tgcggccgta tacggcacga aatgctcggc gccgccggcg cgcagcacgc gcagcgncat cgatgcgcag ttttccttct tgctgatcat ttgatacatg agctcgcctg acttcncgcc ttccttcacg gtgcgcgcat cgcgcaacat cgccgcctcg ttcagcccga acagcacgaa cgcgcgcggg cggtccgtat cgcgcctgtc gtggttgaat ccgatcgccg gaaaatagac cttgcgcgcg ctcannccca ttcggcatcc ttgnccaact tctggccggc tctcggaaaa accgcgcgtc gtgacgctcg tcctgattcg ngtatttgaa gccgtccgcg aggatctttc tcctcgccgc gtcgcnttcg cgaagcctct gntncntcgc gctngaaatn tccgagcgct ngtcgatccg atagccgtcc agcntnttcg gttcgatgcg atcgaaatgc tccttactnn ccagtcgntg attcgnccac caactgacgt gctcgtacac gtgccggccg gcatcggcgt ccgcctgant cttgatcgtc agcgacgcgt gccccggatg atcgagcccc cggcattccc acgaccgaaa tnnnnnntgc cgggcgagcg gcttcgcgtc gcccgtgccg tggaagtgct cggcgatcga caccatgttg cgcacgcgcc gctgatcgag cggcgcgccg tcgcgccgcg cgccgtagtc gaacgccgcc ttcgcgcctt ccgggccata tcgcttcgac agcgcgagca cgaacacctc gagcgagcgg cggttttcca tgcgaacctg ctccgcatag cgccgcaccg catcgcagtg cttgagcagc ggcacgtgcg cgagaaacgc gaacaaccgc tccttcatcc ccaacctcgc ggccgacacc accgggccgc gcgcgtcgcc gccgacgacg acacgcgcgc cggatcgcgc gcttgcgacg aatgcatcga cattgatcat cgaaccagga ccctgggtct gagcttccag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMINVDAFVA SARSGARVVV GGDARGPVVS AARLGMKERL FAFLAHVPLL KHCDAVRRYA EQVRMENRRS LEVFVLALSK RYGPEGAKAA FDYGARRDGA PLDQRRVRNM VSIAEHFHGT GDAKPLARQM VFRSWECRGL DHPGHASLTI KNQADADAGR HVYEHVSWWP NQRLGSKEHF DRIEPKTLDG YRIDKRSEIS SATEQRLREG DAARRKILAD GFKYANQDER HDARFFPRAG QKLDKDAEWG LSARKVYFPA IGFNHDRRDT DRPRAFVLFG LNEAAMLRDA RTVKEGAKSG ELMYQMISKK ENCASMALRV LRAGGAEHFV PYTAAWISED PNHAHAYALA VQARIDALNQ RRADVERRCE RLRDSASVRQ AWRAFSEAGG ASASPLAEDA GRGRASAHMR QARLDEHARE VERIGAYFAE LSAGRSGKHR DRADAALADA MKRCAPSARD DVAALTRKAS VLVETLGRHL DAPPPSDSSA LRRLAAHAMI GRIEAFMAAA IAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatcaatgtc gatgcattcg tcgcaagcgc gcgatccggc gcgcgtgtcg tcgtcggcgg cgacgcgcgc ggcccggtgg tgtcggccgc gaggttgggg atgaaggagc ggttgttcgc gtttctcgcg cacgtgccgc tgctcaagca ctgcgatgcg gtgcggcgct atgcggagca ggttcgcatg gaaaaccgcc gctcgctcga ggtgttcgtg ctcgcgctgt cgaagcgata tggcccggaa ggcgcgaagg cggcgttcga ctacggcgcg cggcgcgacg gcgcgccgct cgatcagcgg cgcgtgcgca acatggtgtc gatcgccgag cacttccacg gcacgggcga cgcgaagccg ctcgcccggc aaatggtatt tcggtcgtgg gaatgccggg ggctcgatca tccggggcac gcgtcgctga cgatcaagaa tcaggcggac gccgatgccg gccggcacgt gtacgagcac gtcagttggt ggccgaatca acgactgggt agtaaggagc atttcgatcg catcgaaccg aagacgctgg acggctatcg gatcgacaag cgctcggaga tttcgagcgc gacggaacag aggcttcgcg aaggcgacgc ggcgaggaga aagatcctcg cggacggctt caaatacgcg aatcaggacg agcgtcacga cgcgcggttt tttccgagag ccggccagaa gttggacaag gatgccgaat gggggctgag cgcgcgcaag gtctattttc cggcgatcgg attcaaccac gacaggcgcg atacggaccg cccgcgcgcg ttcgtgctgt tcgggctgaa cgaggcggcg atgttgcgcg atgcgcgcac cgtgaaggaa ggcgcgaagt caggcgagct catgtatcaa atgatcagca agaaggaaaa ctgcgcatcg atggcgctgc gcgtgctgcg cgccggcggc gccgagcatt tcgtgccgta tacggccgca tggatctccg aggatccgaa tcacgcgcac gcctatgcgc tggccgtgca ggcgaggatc gacgcgctga accagcggcg tgccgacgtc gagcgccgct gcgagcggtt gcgcgacagc gcatcggtgc ggcaggcgtg gcgcgcgttc tccgaggccg gcggtgcctc ggcaagcccg ctcgcggagg acgccggccg cgggcgagca tcggcgcaca tgcggcaggc gcggctcgac gagcatgcgc gcgaggtcga gcgaatcggc gcgtatttcg ccgagctgtc cgccgggcga tccggcaagc atcgcgatcg ggcggatgcg gctctggccg atgcgatgaa gcgttgcgcg ccttcggctc gcgacgacgt cgcggcgctg acgcgcaagg cgagcgtgct cgtcgagacg ctcggccggc atctcgacgc gccgccgccg agcgattcgt ccgcgctgag gaggctggcc gcgcacgcga tgatcggccg gatcgaggcg ttcatggcgg ctgcgatcgc cgcctgaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BupsA.01674.a.A1.PS01851

PURIFICATION DATe:	6/20/2013
CONCENTRATION:	11.6mg/ml
OBSERVED MW:	62kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	1
VIAL VOLUME:	110µl
PERCENT IDENTITY:	57
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCAATGTC GATGCATTCG TCGCAAGCGC GCGATCCGGC GCGCGTGTCG TCGTCGGCGG CGACGCGCGC GGCCCGGTGG TGTCGGCCGC GAGGTTGGGG ATGAAGGAGC GGTTGTTCGC GTTTCTCGCG CACGTGCCGC TGCTCAAGCA CTGCGATGCG GTGCGGCGCT ATGCGGAGCA GGTTCGCATG GAAAACCGCC GCTCGCTCGA GGTGTTCGTG CTCGCGCTGT CGAAGCGATA TGGCCCGGAA GGCGCGAAGG CGGCGTTCGA CTACGGCGCG CGGCGCGACG GCGCGCCGCT CGATCAGCGG CGCGTGCGCA ACATGGTGTC GATCGCCGAG CACTTCCACG GCACGGGCGA CGCGAAGCCG CTCGCCCGGC AAATGGTATT TCGGTCGTGG GAATGCCGGG GGCTCGATCA TCCGGGGCAC GCGTCGCTGA CGATCAAGAA TCAGGCGGAC GCCGATGCCG GCCGGCACGT GTACGAGCAC GTCAGTTGGT GGCCGAATCA ACGACTGGGT AGTAAGGAGC ATTTCGATCG CATCGAACCG AAGACGCTGG ACGGCTATCG GATCGACAAG CGCTCGGAGA TTTCGAGCGC GACGGAACAG AGGCTTCGCG AAGGCGACGC GGCGAGGAGA AAGATCCTCG CGGACGGCTT CAAATACGCG AATCAGGACG AGCGTCACGA CGCGCGGTTT TTTCCGAGAG CCGGCCAGAA GTTGGACAAG GATGCCGAAT GGGGGCTGAG CGCGCGCAAG GTCTATTTTC CGGCGATCGG ATTCAACCAC GACAGGCGCG ATACGGACCG CCCGCGCGCG TTCGTGCTGT TCGGGCTGAA CGAGGCGGCG ATGTTGCGCG ATGCGCGCAC CGTGAAGGAA GGCGCGAAGT CAGGCGAGCT CATGTATCAA ATGATCAGCA AGAAGGAAAA CTGCGCATCG ATGGCGCTGC GCGTGCTGCG CGCCGGCGGC GCCGAGCATT TCGTGCCGTA TACGGCCGCA TGGATCTCCG AGGATCCGAA TCACGCGCAC GCCTATGCGC TGGCCGTGCA GGCGAGGATC GACGCGCTGA ACCAGCGGCG TGCCGACGTC GAGCGCCGCT GCGAGCGGTT GCGCGACAGC GCATCGGTGC GGCAGGCGTG GCGCGCGTTC TCCGAGGCCG GCGGTGCCTC GGCAAGCCCG CTCGCGGAGG ACGCCGGCCG CGGGCGAGCA TCGGCGCACA TGCGGCAGGC GCGGCTCGAC GAGCATGCGC GCGAGGTCGA GCGAATCGGC GCGTATTTCG CCGAGCTGTC CGCCGGGCGA TCCGGCAAGC ATCGCGATCG GGCGGATGCG GCTCTGGCCG ATGCGATGAA GCGTTGCGCG CCTTCGGCTC GCGACGACGT CGCGGCGCTG ACGCGCAAGG CGAGCGTGCT CGTCGAGACG CTCGGCCGGC ATCTCGACGC GCCGCCGCCG AGCGATTCGT CCGCGCTGAG GAGGCTGGCC GCGCACGCGA TGATCGGCCG GATCGAGGCG TTCATGGCGG CTGCGATCGC CGCCTGAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA