BupsA.01671.a

Sensor protein (EC 2.7.13.3)

CENTER ID:	BupsA.01671.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.01671.a.A1.GE34748	periplasmic domain		62	307

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_192992
PATRIC ID:	fig\|320372.6.peg.1932
RefSeq:	YP_333244.1
UniProt:	Q3JT59

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRFDRTQRRR LLGRRFLVEW IAIGCLGVAV ILAGVAGRMT ASVDRIIYDR LLTLRKLPIE SNIVIVEIDN RSLDTLGRWP WQRSVHAEFL GALAKTHPAA VVYDVLFTEP SADDRALAKV LDLVPTFLPV LLSPEEPDGT RTVNPPVPVL AQHVAGAGHI NLEVDPDGIV RSVALFESDG LHRWPQLMVP VFHALENGQF KLAQPAPIAR RAHDIAQDDN GESRYFVPFS RASQNYPAIS FVDVLAGRVN ADELRGKIVI VGATASGLYD RFATPISGEF GPLPGVYIHA SVLDTLMTGR AISPVSGWLV FSASLLPLVV LLAGFLMLSP WRSLLLTLSL AALAVASSAA LLYEARLWLS PAPAIFGLIA AYPIWNWRRL EMTMSYLRYE LQRLADEPHL LPEVPRERNE FGGDVLERQM ALMAQAAQRV QDMKRFVWDS LDSMPEPVVV TDLAGTVLIA NHAAKRYCAR LDAPAPEGRP LRVAFGELSF VKTVDGNAEQ DVAIRTHWPA ALDPTRDYEN DVMERGIEVR DRSGLDHLLR YAPCTNAQGN VTGWIAGLVD VTALHAAERQ REEALHLLSH DMRSPQSSIL ALVEIERQRA ESEHTRGLLA RIERYAQRAL MLADEFVQLA RAESQTYQLE AVSFADLLID ASDEVWPQAQ AKRIRIDTSF GDEPCWIGAD RSLMTRALVN LLNNAVKYSP TDTVITCTLS VEPGAKRVHC TIRDQGYGIS LEDQRHLFER FKRFHANERP EVAGSGLGMA FVKTVVVRHG GCIGVDSTPG IGTAITVSLP LLDEPAA

NT Sequence

atgagattcg accgcacgca acggcggcgg ctgctcggcc gccgttttct cgtcgagtgg atcgcgatcg gctgcctcgg ggtcgcggtg attctcgccg gcgtcgccgg ccggatgacg gcgagcgtcg accggatcat ctacgatcgc ctgctcacgc tgcgcaagct gccgatcgag tcgaacatcg tgatcgtcga gatcgacaat cgcagcctcg acacgctcgg gcgctggcca tggcagcgca gcgtccatgc ggaattcctc ggcgcgctcg cgaagacgca tccggcggcc gtcgtctacg acgtgctgtt taccgagccg tccgccgacg accgcgcgct cgcgaaggtg ctcgatctcg tgccgacgtt cctgcccgtg ctgctgagcc cggaggagcc cgacggcacg cgcaccgtca atccgcccgt gccggtgctc gcgcagcacg tcgcgggcgc gggccacatc aatctcgaag tcgatccgga cggcatcgtg cgcagcgtcg cgctgttcga gagcgacggg ctgcaccgct ggccgcagtt gatggtgccc gtgttccacg cgctcgagaa cggccagttc aagctcgcgc agcccgcgcc gatcgcgcgg cgcgcgcacg acatcgcgca ggacgacaac ggcgagtcgc gctatttcgt cccgttcagc cgcgcgtcgc agaactaccc ggcgatttcg ttcgtcgacg tgctcgcggg ccgcgtgaac gccgacgagc tgcgcggcaa gatcgtgatc gtcggcgcga ccgcgtcggg cctctacgac cgcttcgcga cgccgatctc gggcgagttc gggccgctgc cgggcgtgta catccatgcg agcgtgctcg atacgctgat gacgggccgc gcgatctcgc ccgtgtccgg ctggctcgtg ttcagcgcgt cgctgctgcc gctcgtcgtg ctgctcgcgg gcttcctgat gctctcgccg tggcgctcgc tgctcctcac gctgagcctt gccgcgctcg cggtcgcctc gagcgcggcg ctcctctacg aggcgcggct gtggctgtcg cccgcgccgg cgatcttcgg gctgatcgcc gcgtacccga tctggaactg gcggcgcctc gagatgacga tgtcgtacct gcgctacgag ctgcagcgcc tcgccgacga gccgcacctg ctgcccgagg tgccgcgcga gcgcaacgaa ttcggcggcg acgtgctcga gcgtcagatg gcgctgatgg cgcaggccgc gcagcgcgtg caggacatga agcgcttcgt ctgggacagc ctcgacagca tgccggagcc cgtcgtcgtg accgatctcg cgggcacggt gctgatcgcc aatcatgcgg cgaagcgcta ctgcgcgcgg ctagacgctc ccgcgccgga agggcggccg ctgcgcgtcg cgttcggcga gctgtcgttc gtgaagacgg tcgacggcaa cgccgagcag gacgtcgcga tccgcacgca ctggccggcc gcgctcgacc cgacgcgcga ctacgagaac gacgtgatgg agcgcggcat cgaggtgcgc gaccgcagcg gcctcgatca tctgctacgc tacgcgccgt gcacgaacgc gcagggcaac gtgacgggct ggatcgccgg cctcgtcgac gtgaccgcgc tgcatgcggc cgagcggcag cgcgaggagg cgctgcatct gctgtcgcac gacatgcgct cgccgcaatc gtcgattctc gcgctcgtcg agatcgagcg gcagcgggcc gaatccgagc acacgcgcgg gctgctcgcg cgcatcgagc gctacgcgca gcgtgcgctg atgctcgccg acgagttcgt gcagctcgcg cgcgcggaat cgcagacgta ccagctcgag gcggtgagct tcgccgattt gctgatcgac gcgagcgacg aagtgtggcc gcaggcgcag gccaagcgca tccgcatcga cacgtcgttc ggcgacgagc cgtgctggat cggcgccgac cgctcgctga tgacccgcgc gctcgtgaat ctgttgaaca atgcggtcaa atacagcccg acggacacgg tgatcacgtg tacgctatca gtcgagccgg gcgcgaagcg cgtacattgc acgatccgcg accagggcta cggcatttcg ctcgaggatc agcggcatct gttcgagcgt ttcaagcggt ttcatgcgaa cgagcggccg gaggtcgcgg gctccgggct cggcatggcg ttcgtcaaga ccgtggtcgt gcggcacggc ggttgcatcg gcgtcgacag cacgccgggc atcggcacgg cgatcaccgt gtcgctgccg ttgctcgacg agccggcggc gtga

Details for BupsA.01671.a.A1.GE34748

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNIVIVEID NRSLDTLGRW PWQRSVHAEF LGALAKTHPA AVVYDVLFTE PSADDRALAK VLDLVPTFLP VLLSPEEPDG TRTVNPPVPV LAQHVAGAGH INLEVDPDGI VRSVALFESD GLHRWPQLMV PVFHALENGQ FKLAQPAPIA RRAHDIAQDD NGESRYFVPF SRASQNYPAI SFVDVLAGRV NADELRGKIV IVGATASGLY DRFATPISGE FGPLPGVYIH ASVLDTLMTG RAISPVSG

Validated NT Sequence

ttattagccg gacacgggcg agatcgcgcg gcccgtcatc agcgtatcga gcacgctcgc atggatgtac acgcccggca gcggcccgaa ctcgcccgag atcggcgtcg cgaagcggtc gtagaggccc gacgcggtcg cgccgacgat cacgatcttg ccgcgcagct cgtcggcgtt cacgcggccc gcgagcacgt cgacgaacga aatcgccggg tagttctgcg acgcgcggct gaacgggacg aaatagcgcg actcgccgtt gtcgtcctgc gcgatgtcgt gcgcgcgccg cgcgatcggc gcgggctgcg cgagcttgaa ctggccgttc tcgagcgcgt ggaacacggg caccatcaac tgcggccagc ggtgcagccc gtcgctctcg aacagcgcga cgctgcgcac gatgccgtcc ggatcgactt cgagattgat gtggcccgcg cccgcgacgt gctgcgcgag caccggcacg ggcggattga cggtgcgcgt gccgtcgggc tcctccgggc tcagcagcac gggcaggaac gtcggcacga gatcgagcac cttcgcgagc gcgcggtcgt cggcggacgg ctcggtaaac agcacgtcgt agacgacggc cgccggatgc gtcttcgcga gcgcgccgag gaattccgca tggacgctgc gctgccatgg ccagcgcccg agcgtgtcga ggctgcgatt gtcgatctcg acgatcacga tgttcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacatcgtg atcgtcgaga tcgacaatcg cagcctcgac acgctcgggc gctggccatg gcagcgcagc gtccatgcgg aattcctcgg cgcgctcgcg aagacgcatc cggcggccgt cgtctacgac gtgctgttta ccgagccgtc cgccgacgac cgcgcgctcg cgaaggtgct cgatctcgtg ccgacgttcc tgcccgtgct gctgagcccg gaggagcccg acggcacgcg caccgtcaat ccgcccgtgc cggtgctcgc gcagcacgtc gcgggcgcgg gccacatcaa tctcgaagtc gatccggacg gcatcgtgcg cagcgtcgcg ctgttcgaga gcgacgggct gcaccgctgg ccgcagttga tggtgcccgt gttccacgcg ctcgagaacg gccagttcaa gctcgcgcag cccgcgccga tcgcgcggcg cgcgcacgac atcgcgcagg acgacaacgg cgagtcgcgc tatttcgtcc cgttcagccg cgcgtcgcag aactacccgg cgatttcgtt cgtcgacgtg ctcgcgggcc gcgtgaacgc cgacgagctg cgcggcaaga tcgtgatcgt cggcgcgacc gcgtcgggcc tctacgaccg cttcgcgacg ccgatctcgg gcgagttcgg gccgctgccg ggcgtgtaca tccatgcgag cgtgctcgat acgctgatga cgggccgcgc gatctcgccc gtgtccggca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a