BupsA.01669.a

Sensor protein (EC 2.7.13.3)

CENTER ID:	BupsA.01669.a
ORGANISM:	Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE:	Melioidosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	True
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.01669.a.A1.GE26317	periplasmic domain		99	323

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BupsA.01669.a.A1.PS00265	periplasmic domain		99	323

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127115
PATRIC ID:	fig\|320372.6.peg.2873
RefSeq:	YP_334133.1
UniProt:	Q3JQM0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRNIAAPHRL DPRLPCVVYR QFRFRFNLSC TPFVQDALSY NPAMLTDRLF ARSARPSGSP AESQPSRWHH GPWWSNSYLL TPLLSILVFL VVMSLILWSL NRREEQQQED TLFRNVAWAQ QQIRLSMTSA QEQLQAFSRD IAAGRIDEHA FQATVGDVMQ AHPEILYLNW YTSPGTKRWP TMQLPLLGQR LAKPNDAQMD EVVRGAYAQA RGTRRQSYSP LVYDDFGNGY LTLQTPVIRE REYLGSIAAV FSVEGILKHD IPPELSAKYK ISITDANNRE LASTSSRPRL PRDAHYDLPL DPPGQGLTVR VYAYPQTTNL TNNTLVWLVA GLSCFVLWSL WSLWKHTRQR FEAQQALYAE AFFRRAMENS VLIGMRVLDM HGRITHVNPA FCRMTGWDES DLVGKTAPFP YWPRDAYPEM QRQLDMTLRG KAPSSGFELR VRRKDGSLFH ARLYVSPLID SAGRQTGWMS SMTDITEPKR AREELAAAHE RFTTVLESLD AAVSVLAADE AELLFANRYY RHLFGIRPDG HLELSGGGFD TAQASSDSID MVDAYAGLPA AALTESTADA QEVYVESIQK WFEVRRQYIQ WVDGHLAQMQ IATDITTRKK AQELAHQQEE KLQFTSRLMT MGEMASSIAH ELNQPLAAIN NYCSGTLALV KSGRASPETL APALEKTAQQ ALRAGMIVKR IREFVKRSEP KRQPSRVADI VADAVGLAEI EARKRRIRIV TEIRARMPII YVDPVLIEQV LVNLMKNAAE AMQEARPQAE NGVIRVVADL EAGFVDIRVI DQGPGVDEAT AERLFEPFYS TKSDGMGMGL NICRSIIESH RGRLWVVNNV EPDGLVSGAT FHCSLPIGEP EDLGRGSETS PSQTVTGEI

NT Sequence

atgcggaata ttgctgcgcc gcaccgactc gacccgcgtt tgccatgcgt cgtgtatcga caattccgct ttcggttcaa cctttcttgt acgccttttg tgcaggacgc gctgagctac aatcctgcca tgttgaccga tcggcttttc gctcgctcgg cgcgaccgtc gggctcgcct gcggagtcgc agccgtcccg ctggcaccac ggaccgtggt ggtccaactc ttacctgctc acccccctgc tgtcgatcct cgtctttctc gtggtgatga gtctcattct gtggagcctc aatcgccgcg aggagcagca gcaggaagac acgctgtttc gcaacgtcgc gtgggcgcag cagcagatcc gcctgtcgat gacgagcgcg caggaacagc tccaggcgtt ctcgcgcgac atcgccgcgg gccgcatcga cgagcatgcg ttccaggcga cggtgggcga cgtgatgcag gcgcaccccg agatcctcta tctgaactgg tacacgtcgc ccggcacgaa acgctggccg acgatgcaat tgccgctcct cggccagcgg ctcgcgaagc cgaacgacgc gcagatggac gaagtcgtgc gcggcgcgta cgcgcaggcg cgcggcacgc gccgccagtc gtactcgccg ctcgtctacg acgacttcgg caacggctac ctgacgctgc aaacgcccgt gatccgcgag cgcgagtatc tcggctcgat cgccgcggtg ttctcggtcg aaggcatcct gaagcacgac atcccgcccg agctgtccgc gaaatacaag atctcgatca ccgacgcgaa caaccgcgag ctcgcatcga cgtcgtcgcg cccgcggctg ccgcgcgacg cgcattacga cctgccgctc gacccgcccg gccagggcct cacggtacgc gtctacgcgt acccgcaaac gacgaacctg accaacaaca cgctcgtatg gctcgtcgcg ggcctgtcgt gcttcgtgct gtggagcctc tggagcttgt ggaagcacac gcggcagcgc ttcgaggcgc agcaggcgct gtacgccgag gcgttcttcc gccgcgcgat ggagaattcg gtgctgatcg gcatgcgcgt gctcgacatg cacggccgga tcacgcacgt gaacccggcg ttctgccgga tgacgggctg ggacgaaagc gacctcgtcg gcaagaccgc gccgttcccg tactggccgc gcgacgctta cccggaaatg cagcgccagc tcgacatgac gctgcgcggc aaggcgcctt cgtccggctt cgagctgcgc gtgcgccgca aggacggctc gctctttcac gcacgcctgt acgtatcgcc gctcatcgac agcgccggcc ggcagacggg ctggatgtcg tcgatgaccg acatcaccga gcccaagcgc gcgcgcgagg agctcgcggc cgcgcacgag cgcttcacga cagtgctcga gagcctcgac gccgcggtgt cggtgctcgc cgcggacgaa gccgagctgc tgttcgcgaa ccgctactac cggcacctgt tcggcatccg cccggacggc cacctcgaac tgtcgggcgg cggcttcgac accgcgcagg cgtcgtccga ttcgatcgac atggtcgacg cctatgccgg cctgcccgcc gcggcgctca ccgagagcac ggcggacgcg caggaggtgt acgtcgagag catccagaag tggttcgagg tgcgccgcca gtacatccag tgggtcgacg gccacctcgc gcagatgcag atcgcgaccg acatcacgac gcgcaagaag gcgcaggagc tcgcgcacca gcaggaagaa aagctgcagt tcacgagccg gctgatgacg atgggtgaaa tggcgtcgtc gattgcacac gaactgaacc agccgctcgc ggcgatcaac aactactgct cgggcacgct cgcgctcgtg aagagcggcc gcgcgtcgcc ggagacgctc gcgcccgcgc tcgagaagac cgcgcagcag gcgctgcgcg ccgggatgat cgtcaagcgg atccgcgagt tcgtcaagcg cagcgagccg aagcggcagc cgtcgcgggt cgccgacatc gtcgccgacg cggtcgggct cgccgaaatc gaggcgagaa agcgccggat tcggatcgtc accgaaatcc gcgcaagaat gcctattatt tatgtcgacc ccgtgttgat cgagcaggtg ctcgtgaacc tgatgaagaa cgcggccgag gcgatgcagg aggcgcggcc gcaggcggag aacggcgtga tccgcgtcgt cgccgacctc gaggcgggtt tcgtcgacat tcgcgtgatc gaccagggtc cgggcgtcga cgaggcgacg gccgaacgcc tgttcgaacc gttctacagc accaagtcgg acgggatggg catggggctc aatatctgcc gctcgatcat cgaatcgcat cgcgggcgtc tgtgggtggt caacaacgtc gagccggacg gcctcgtgtc gggcgcgacg tttcactgca gcctgcccat tggggaaccg gaggatctcg gtcgcggatc cgagacatcg ccatcacaaa ccgtaacggg agagatatga

Details for BupsA.01669.a.A1.GE26317

HARVESTED ON:	3/10/2009
SEQUENCED ON:	3/26/2009
EXPECTED MW:	27kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSLNRREEQ QQEDTLFRNV AWAQQQIRLS MTSAQEQLQA FSRDIAAGRI DEHAFQATVG DVMQAHPEIL YLNWYTSPGT KRWPTMQLPL LGQRLAKPND AQMDEVVRGA YAQARGTRRQ SYSPLVYDDF GNGYLTLQTP VIREREYLGS IAAVFSVEGI LKHDIPPELS AKYKISITDA NNRELASTSS RPRLPRDAHY DLPLDPPGQG LTVRVYAYPQ TTNLT

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagcc tcaatcgccg cgaggagcag cagcaggaag acacgctgtt tcgcaacgtc gcgtgggcgc agcagcagat ccgcctgtcg atgacgagcg cgcaggaaca gctccaggcg ttctcgcgcg acatcgccgc gggccgcatc gacgagcatg cgttccaggc gacggtgggc gacgtgatgc aggcgcaccc cgagatcctc tatctgaact ggtacacgtc gcccggcacg aaacgctggc cgacgatgca attgccgctc ctcggccagc ggctcgcgaa gccgaacgac gcgcagatgg acgaagtcgt gcgcggcgcg tacgcgcagg cgcgcggcac gcgccgccag tcgtactcgc cgctcgtcta cgacgacttc ggcaacggct acctgacgct gcaaacgccc gtgatccgcg agcgcgagta tctcggctcg atcgccgcgg tgttctcggt cgaaggcatc ctgaagcacg acatcccgcc cgagctgtcc gcgaaataca agatctcgat caccgacgcg aacaaccgcg agctcgcatc gacgtcgtcg cgcccgcggc tgccgcgcga cgcgcattac gacctgccgc tcgacccgcc cggccagggc ctcacggtac gcgtctacgc gtacccgcaa acgacgaacc tgacca

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcctcaat cgccgcgagg agcagcagca ggaagacacg ctgtttcgca acgtcgcgtg ggcgcagcag cagatccgcc tgtcgatgac gagcgcgcag gaacagctcc aggcgttctc gcgcgacatc gccgcgggcc gcatcgacga gcatgcgttc caggcgacgg tgggcgacgt gatgcaggcg caccccgaga tcctctatct gaactggtac acgtcgcccg gcacgaaacg ctggccgacg atgcaattgc cgctcctcgg ccagcggctc gcgaagccga acgacgcgca gatggacgaa gtcgtgcgcg gcgcgtacgc gcaggcgcgc ggcacgcgcc gccagtcgta ctcgccgctc gtctacgacg acttcggcaa cggctacctg acgctgcaaa cgcccgtgat ccgcgagcgc gagtatctcg gctcgatcgc cgcggtgttc tcggtcgaag gcatcctgaa gcacgacatc ccgcccgagc tgtccgcgaa atacaagatc tcgatcaccg acgcgaacaa ccgcgagctc gcatcgacgt cgtcgcgccc gcggctgccg cgcgacgcgc attacgacct gccgctcgac ccgcccggcc agggcctcac ggtacgcgtc tacgcgtacc cgcaaacgac gaacctgacc aacaacaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa

Details for BupsA.01669.a.A1.PS00265

PURIFICATION DATe:	5/15/2009
CONCENTRATION:	8.5mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCCTCAAT CGCCGCGAGG AGCAGCAGCA GGAAGACACG CTGTTTCGCA ACGTCGCGTG GGCGCAGCAG CAGATCCGCC TGTCGATGAC GAGCGCGCAG GAACAGCTCC AGGCGTTCTC GCGCGACATC GCCGCGGGCC GCATCGACGA GCATGCGTTC CAGGCGACGG TGGGCGACGT GATGCAGGCG CACCCCGAGA TCCTCTATCT GAACTGGTAC ACGTCGCCCG GCACGAAACG CTGGCCGACG ATGCAATTGC CGCTCCTCGG CCAGCGGCTC GCGAAGCCGA ACGACGCGCA GATGGACGAA GTCGTGCGCG GCGCGTACGC GCAGGCGCGC GGCACGCGCC GCCAGTCGTA CTCGCCGCTC GTCTACGACG ACTTCGGCAA CGGCTACCTG ACGCTGCAAA CGCCCGTGAT CCGCGAGCGC GAGTATCTCG GCTCGATCGC CGCGGTGTTC TCGGTCGAAG GCATCCTGAA GCACGACATC CCGCCCGAGC TGTCCGCGAA ATACAAGATC TCGATCACCG ACGCGAACAA CCGCGAGCTC GCATCGACGT CGTCGCGCCC GCGGCTGCCG CGCGACGCGC ATTACGACCT GCCGCTCGAC CCGCCCGGCC AGGGCCTCAC GGTACGCGTC TACGCGTACC CGCAAACGAC GAACCTGACC AACAACAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA