BupsA.01666.a

Sensor protein (EC 2.7.13.3)

CENTER ID: BupsA.01666.a
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.01666.a.A1.GE26316 periplasmic domain 53 320
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.01666.a.A1.PW30865a periplasmic domain 53 320
BupsA.01666.a.A1.PW30865b periplasmic domain 53 320
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_155568
PATRIC ID: fig|320372.6.peg.2322
RefSeq: YP_333619.1
UniProt: Q3JS34
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQGLLQELDG SPLRKFYSLE SNLKRERRVF TIVIVLLVCA AFSIAAMTVT GLFQTAFRQE EQSARIHEKE VVDVFLQRRM MLTTASLVLQ LRMNGAPSAL NVPAPNACTP MAHNVRDDAI LRESCDYTVQ LLANSGQTPS VEMVTADGSV GYGYLFPTGD LSALRSSTPS ELVSAVLERY GKRGLDPLEA ARKKRILWFA VGRGGRGEEL HMIGASVVFK DERLYALVLT SVDLHSLVSP IERAGRVQQP VVVDSEGVPL VNADDAETVR KVDGRLAGQQ DGLYHWIPGF GWALRRPAPF SGFGHMTYLL PLDLQLRSMR YELSLVGGAT LVLIVLLFVA FRYWNYRFLT RIYEEASRAL ESEMLNHLLV HATPVGLCIV RRATLEIVVA NPIARTMLGL RLSDRHLPQE LLSAFESSLA EQDTQSDDAR IFQFPFTLSR AGHAAVHIEI TYAPAMLNAR EVFFCAITDM TAHHQAEILL REAKLTSDAA AKAKVAFFAS MSHEIRTPLS SLVGNIELIA RGPLAPEQQA RVKAMETSAR GLMQIVNDVL DFSKIDVGEL SLMEEWSNIA ELLDRLALSH APLATQQGLK FYMVFDRSLP ARLYFDPIRV SQIVNNLLSN ALKFTPSGKI VLRAGWRAGA LEISVTDSGI GIPDDLKHRL FLPFTQGDSN RLRQARGTGL GLSICARLCE LMKGRIDLES TVGVGTRIAV TLPLGVSEAD SSDAYWTLPY RRVAVLGRAQ ENLEWLANLF DPGVTAVTAF SRPAEPIDAH AHDFLMVTDE FSPAEVLPWW RRPDSIVWVG QAGPLVPRRR DDGGVEISMY SLAGLKSATH MLAAGRTALA EAGHEPPGAE AGMTVLIAED NLLNRSLLLD QLTTLGVRVI EAKNGEEALA LLLKEPVDVV MTDIDMPMMD GFQLLAEMRR LGMTMPVYAV SASARPEDVA EGRARGFTDY LAKPVSLERL ETVVRACCSA PAGARADEDA QDELPGLPDV PPAYASAFVA QAGSEIAEFD AILRERALPK LRRWLHGVSG GIAVLGPSAL HEQCQELRAY ARESGEWNRE IELQALAIRN ALERMVAALT SA
NT Sequence
atgcaaggac ttctgcaaga gctcgacgga tcgccgctga ggaagttcta ttcgctcgag tcgaatctga agcgcgagcg gcgggtcttc acgatcgtca tcgtgctgct cgtctgcgcg gccttcagca tcgcggccat gaccgtcacc ggcttgttcc agaccgcttt ccggcaggag gagcaatccg cgcgcatcca cgaaaaggaa gtggtcgacg tgttcctgca gcgccgcatg atgttgacga cggcaagcct cgtgctgcaa ctgcggatga acggcgcgcc ttcggcgctg aacgtgccgg cgccgaacgc gtgcacgccg atggcccaca atgtgcgcga cgatgcgatc ctgcgcgaga gctgcgatta cacggtgcag ttgctggcca actcggggca gacgccgagc gtcgaaatgg tgacggccga cggttcggtc ggctatggat atctgtttcc gacgggcgac ctgagcgcgc tacgctccag cacgccgtcc gaactcgtgt cggccgtgct cgagcgctac ggcaagcgcg gcctggaccc gctggaggcc gcgcgcaaga agcggattct ctggttcgcg gtgggccgcg gcgggcgcgg cgaggagctg catatgatcg gcgcgtcagt cgtgttcaag gacgagcggc tctacgcgct cgtcttgacg agcgtggatc ttcacagcct cgtttcgccg atcgagcgcg ccggccgcgt gcagcagccg gtcgtcgtgg attccgaggg cgtgccgctc gtgaacgcgg acgacgcgga aacggtccgg aaggtcgacg ggcggctcgc cggacaacag gatggcctgt atcactggat tcccggcttc gggtgggccc tacgccgtcc cgcgccgttt tccggtttcg ggcacatgac gtatctgctt ccgctcgatc tgcagttgcg ctcgatgcgc tacgagttga gcctcgtcgg cggcgcgacg ctcgtgctga tcgtattgct gttcgtcgcg ttccgatact ggaattaccg gttcttgacg cgcatctacg aggaagcgtc gcgcgcgctc gagagcgaaa tgctcaacca tctgctggtt catgcgacgc cggtcgggtt gtgcatcgtg cggcgggcga cactggagat cgtcgtcgcc aacccgatcg cgcgcacgat gctcggcttg cggctgtcgg atcggcacct gccgcaggaa ttgctgagcg cgttcgaatc gtcgctggcc gagcaggaca cccagtcgga cgacgcgcgc attttccagt tcccgttcac gctgtcgcgc gccgggcatg cggcggtcca tatcgaaatc acgtacgcgc ccgcgatgct gaacgcgcgg gaggtgttct tttgcgcgat caccgacatg acggcgcacc accaggcgga gatcctgctg cgcgaggcga agctgacgag cgacgcggcg gccaaggcga aggtggcgtt cttcgcatcg atgagccatg aaatccgcac gccgctgtcg tcgctcgtgg gcaacatcga gctgatcgcg cgcgggccgc tcgcgcccga gcagcaggcg cgcgtgaagg cgatggagac gtcggcgcgc ggcttgatgc agatcgtcaa cgatgtgctc gatttctcga agatcgacgt gggcgagctg agcctcatgg aggagtggtc gaacatcgcc gagctgctcg accggctcgc gctctcgcac gcgccgctcg cgacgcagca gggtttgaag ttctacatgg tgttcgatcg cagcctgccc gcgcggctct acttcgatcc gatccgggtc tcgcagatcg tgaacaatct gctgagcaac gcgctgaagt tcacgccgtc cggcaagatc gtgctgcgcg ccggctggcg tgccggcgcg ctcgaaatca gcgtgacgga ttccggcatc ggcatccccg atgacctgaa gcaccgcctc ttcctgcctt tcacgcaggg cgacagcaac cggctgcggc aggcacgcgg caccggcctc ggattgtcga tctgcgcgcg tctgtgcgaa ctgatgaaag ggcgcatcga tctggaaagc actgtcggcg tgggaacccg gatcgcggtg acactgccgc tcggcgtgtc ggaggccgat tcgagcgatg cgtactggac gcttccgtat cggcgcgtgg ccgtgctcgg tcgcgcacag gaaaatctcg agtggctggc caacctgttc gacccgggcg tcacggccgt gacggctttc tcgcggccgg ccgagccgat cgatgcgcac gcgcacgatt tcctgatggt caccgacgaa ttctcgccgg ccgaggtgct gccgtggtgg aggcggccgg actcgatcgt gtgggtcggg caggccggcc cgctcgtgcc gagacggcgc gacgacggcg gagtggaaat cagcatgtat agcctcgcgg ggctgaaatc cgcgactcac atgctcgcgg ccggccgcac ggcgctcgcc gaagcggggc acgagccgcc gggagccgag gcgggaatga cggtgctgat cgccgaggac aatctgctca accgcagcct gctgctcgat cagctgacga cgctgggcgt gcgggtcatc gaggcgaaga acggcgagga ggcgctcgcg ttgctgttga aggagccggt ggacgtcgtg atgaccgaca tcgacatgcc gatgatggac ggtttccagt tgctcgccga gatgaggcgg ctcggcatga cgatgccggt gtacgcggtg agtgcgagcg cgcggccgga agatgtggcg gaaggccggg cgcgcggctt taccgactat ctcgcgaagc cggtttcgct cgagcggctc gagacggtgg tacgcgcatg ctgcagcgcg ccggcgggcg cgcgcgccga cgaagacgcg caggacgaac tgccgggcct acccgacgtg ccgcccgcct atgcgagcgc gttcgtcgcg caggccggca gcgaaatcgc ggaattcgac gcgatcctgc gcgaacgcgc gctgccgaaa ctgcggcggt ggctgcacgg cgtatcgggc ggcatcgcgg tcctcgggcc ttccgcgctg catgagcaat gccaggagct tcgagcctac gcgcgcgaat ccggcgaatg gaatcgcgaa atcgaactgc aggcgctggc cattcggaac gcgctggagc gaatggtcgc ggcgctgacg agcgcgtga
Details for BupsA.01666.a.A1.GE26316
HARVESTED ON: 3/10/2009
SEQUENCED ON: 3/26/2009
EXPECTED MW: 31kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFQTAFRQE EQSARIHEKE VVDVFQQRRM MLTTASLVLQ LRMNGAPSAL NVPAPNACTP MAHNVRDDAI LRESCDYTVQ LLANSGQTPS VEMVTADGSV GYGYLFPTGD LSALRSSTPS ELVSAVLERY GKRGLDPLEA ARKKRILWFA VGRGGRGEEL HMIGASVVFK DERLYALVLT SVDLHSLVSP IERAGRVQQP VVVDSDGVPL VNADDAETVR KVDGRLAGQQ DGLYHWIPGF GWALRRPAPF SGFGHMTYLL PLDLQLRSMR
Validated NT Sequence
ttattagcgc atcgagcgca actgcagatc gagcggaagc agatacgtca tgtgcccgaa accggaaaac ggcgcgggac ggcgtagggc ccacccgaag ccgggaatcc agtgatacag gccatcctgt tgtccggcga gccgcccgtc gaccttccgg accgtttccg cgtcgtccgc gttcacgagc ggcacgccat cggaatccac gacgaccggc tgctgcacgc ggccggcgcg ctcgatcggc gaaacgaggc tgtgaagatc cacgctcgtc aagacgagcg cgtagagccg ctcgtccttg aacacgactg acgcgccgat catatgcagc tcctcgccgc gcccgccgcg gcccaccgcg aaccagagaa tccgcttctt gcgcgcggcc tccagcgggt ccaggccgcg cttgccgtag cgctcgagca cggccgacac gagttcggac ggcgtgctgg agcgtagcgc gctcaggtcg cccgtcggaa acagatatcc atagccgacc gaaccgtcgg ccgtcaccat ttcgacgctc ggcgtctgcc ccgagttggc cagcaactgc accgtgtaat cgcagctctc gcgcaggatc gcatcgtcgc gcacattgtg ggccatcggc gtgcacgcgt tcggcgccgg cacgttcagc gccgaaggcg cgccgttcat ccgcagttgc agcacgaggc ttgccgtcgt caacatcatg cggcgctgct ggaacacgtc gaccacttcc ttttcgtgga tgcgcgcgga ttgctcctcc tgccggaaag cggtctggaa catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFQTAFRQE EQSARIHEKE VVDVFLQRRM MLTTASLVLQ LRMNGAPSAL NVPAPNACTP MAHNVRDDAI LRESCDYTVQ LLANSGQTPS VEMVTADGSV GYGYLFPTGD LSALRSSTPS ELVSAVLERY GKRGLDPLEA ARKKRILWFA VGRGGRGEEL HMIGASVVFK DERLYALVLT SVDLHSLVSP IERAGRVQQP VVVDSEGVPL VNADDAETVR KVDGRLAGQQ DGLYHWIPGF GWALRRPAPF SGFGHMTYLL PLDLQLRSMR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttccagacc gctttccggc aggaggagca atccgcgcgc atccacgaaa aggaagtggt cgacgtgttc ctgcagcgcc gcatgatgtt gacgacggca agcctcgtgc tgcaactgcg gatgaacggc gcgccttcgg cgctgaacgt gccggcgccg aacgcgtgca cgccgatggc ccacaatgtg cgcgacgatg cgatcctgcg cgagagctgc gattacacgg tgcagttgct ggccaactcg gggcagacgc cgagcgtcga aatggtgacg gccgacggtt cggtcggcta tggatatctg tttccgacgg gcgacctgag cgcgctacgc tccagcacgc cgtccgaact cgtgtcggcc gtgctcgagc gctacggcaa gcgcggcctg gacccgctgg aggccgcgcg caagaagcgg attctctggt tcgcggtggg ccgcggcggg cgcggcgagg agctgcatat gatcggcgcg tcagtcgtgt tcaaggacga gcggctctac gcgctcgtct tgacgagcgt ggatcttcac agcctcgttt cgccgatcga gcgcgccggc cgcgtgcagc agccggtcgt cgtggattcc gagggcgtgc cgctcgtgaa cgcggacgac gcggaaacgg tccggaaggt cgacgggcgg ctcgccggac aacaggatgg cctgtatcac tggattcccg gcttcgggtg ggccctacgc cgtcccgcgc cgttttccgg tttcgggcac atgacgtatc tgcttccgct cgatctgcag ttgcgctcga tgcgcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BupsA.01666.a.A1.PW30865a
PURIFICATION DATe: 10/18/2010
CONCENTRATION: 19.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: data unavailable
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFQTAFRQE EQSARIHEKE VVDVFQQRRM MLTTASLVLQ LRMNGAPSAL NVPAPNACTP MAHNVRDDAI LRESCDYTVQ LLANSGQTPS VEMVTADGSV GYGYLFPTGD LSALRSSTPS ELVSAVLERY GKRGLDPLEA ARKKRILWFA VGRGGRGEEL HMIGASVVFK DERLYALVLT SVDLHSLVSP IERAGRVQQP VVVDSDGVPL VNADDAETVR KVDGRLAGQQ DGLYHWIPGF GWALRRPAPF SGFGHMTYLL PLDLQLRSMR
Validated NT Sequence
ttattagcgc atcgagcgca actgcagatc gagcggaagc agatacgtca tgtgcccgaa accggaaaac ggcgcgggac ggcgtagggc ccacccgaag ccgggaatcc agtgatacag gccatcctgt tgtccggcga gccgcccgtc gaccttccgg accgtttccg cgtcgtccgc gttcacgagc ggcacgccat cggaatccac gacgaccggc tgctgcacgc ggccggcgcg ctcgatcggc gaaacgaggc tgtgaagatc cacgctcgtc aagacgagcg cgtagagccg ctcgtccttg aacacgactg acgcgccgat catatgcagc tcctcgccgc gcccgccgcg gcccaccgcg aaccagagaa tccgcttctt gcgcgcggcc tccagcgggt ccaggccgcg cttgccgtag cgctcgagca cggccgacac gagttcggac ggcgtgctgg agcgtagcgc gctcaggtcg cccgtcggaa acagatatcc atagccgacc gaaccgtcgg ccgtcaccat ttcgacgctc ggcgtctgcc ccgagttggc cagcaactgc accgtgtaat cgcagctctc gcgcaggatc gcatcgtcgc gcacattgtg ggccatcggc gtgcacgcgt tcggcgccgg cacgttcagc gccgaaggcg cgccgttcat ccgcagttgc agcacgaggc ttgccgtcgt caacatcatg cggcgctgct ggaacacgtc gaccacttcc ttttcgtgga tgcgcgcgga ttgctcctcc tgccggaaag cggtctggaa catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFQTAFRQE EQSARIHEKE VVDVFLQRRM MLTTASLVLQ LRMNGAPSAL NVPAPNACTP MAHNVRDDAI LRESCDYTVQ LLANSGQTPS VEMVTADGSV GYGYLFPTGD LSALRSSTPS ELVSAVLERY GKRGLDPLEA ARKKRILWFA VGRGGRGEEL HMIGASVVFK DERLYALVLT SVDLHSLVSP IERAGRVQQP VVVDSEGVPL VNADDAETVR KVDGRLAGQQ DGLYHWIPGF GWALRRPAPF SGFGHMTYLL PLDLQLRSMR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTCCAGACC GCTTTCCGGC AGGAGGAGCA ATCCGCGCGC ATCCACGAAA AGGAAGTGGT CGACGTGTTC CTGCAGCGCC GCATGATGTT GACGACGGCA AGCCTCGTGC TGCAACTGCG GATGAACGGC GCGCCTTCGG CGCTGAACGT GCCGGCGCCG AACGCGTGCA CGCCGATGGC CCACAATGTG CGCGACGATG CGATCCTGCG CGAGAGCTGC GATTACACGG TGCAGTTGCT GGCCAACTCG GGGCAGACGC CGAGCGTCGA AATGGTGACG GCCGACGGTT CGGTCGGCTA TGGATATCTG TTTCCGACGG GCGACCTGAG CGCGCTACGC TCCAGCACGC CGTCCGAACT CGTGTCGGCC GTGCTCGAGC GCTACGGCAA GCGCGGCCTG GACCCGCTGG AGGCCGCGCG CAAGAAGCGG ATTCTCTGGT TCGCGGTGGG CCGCGGCGGG CGCGGCGAGG AGCTGCATAT GATCGGCGCG TCAGTCGTGT TCAAGGACGA GCGGCTCTAC GCGCTCGTCT TGACGAGCGT GGATCTTCAC AGCCTCGTTT CGCCGATCGA GCGCGCCGGC CGCGTGCAGC AGCCGGTCGT CGTGGATTCC GAGGGCGTGC CGCTCGTGAA CGCGGACGAC GCGGAAACGG TCCGGAAGGT CGACGGGCGG CTCGCCGGAC AACAGGATGG CCTGTATCAC TGGATTCCCG GCTTCGGGTG GGCCCTACGC CGTCCCGCGC CGTTTTCCGG TTTCGGGCAC ATGACGTATC TGCTTCCGCT CGATCTGCAG TTGCGCTCGA TGCGCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for BupsA.01666.a.A1.PW30865b
PURIFICATION DATe: 10/18/2010
CONCENTRATION: 19.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: data unavailable
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMFQTAFRQE EQSARIHEKE VVDVFQQRRM MLTTASLVLQ LRMNGAPSAL NVPAPNACTP MAHNVRDDAI LRESCDYTVQ LLANSGQTPS VEMVTADGSV GYGYLFPTGD LSALRSSTPS ELVSAVLERY GKRGLDPLEA ARKKRILWFA VGRGGRGEEL HMIGASVVFK DERLYALVLT SVDLHSLVSP IERAGRVQQP VVVDSDGVPL VNADDAETVR KVDGRLAGQQ DGLYHWIPGF GWALRRPAPF SGFGHMTYLL PLDLQLRSMR
Validated NT Sequence
ttattagcgc atcgagcgca actgcagatc gagcggaagc agatacgtca tgtgcccgaa accggaaaac ggcgcgggac ggcgtagggc ccacccgaag ccgggaatcc agtgatacag gccatcctgt tgtccggcga gccgcccgtc gaccttccgg accgtttccg cgtcgtccgc gttcacgagc ggcacgccat cggaatccac gacgaccggc tgctgcacgc ggccggcgcg ctcgatcggc gaaacgaggc tgtgaagatc cacgctcgtc aagacgagcg cgtagagccg ctcgtccttg aacacgactg acgcgccgat catatgcagc tcctcgccgc gcccgccgcg gcccaccgcg aaccagagaa tccgcttctt gcgcgcggcc tccagcgggt ccaggccgcg cttgccgtag cgctcgagca cggccgacac gagttcggac ggcgtgctgg agcgtagcgc gctcaggtcg cccgtcggaa acagatatcc atagccgacc gaaccgtcgg ccgtcaccat ttcgacgctc ggcgtctgcc ccgagttggc cagcaactgc accgtgtaat cgcagctctc gcgcaggatc gcatcgtcgc gcacattgtg ggccatcggc gtgcacgcgt tcggcgccgg cacgttcagc gccgaaggcg cgccgttcat ccgcagttgc agcacgaggc ttgccgtcgt caacatcatg cggcgctgct ggaacacgtc gaccacttcc ttttcgtgga tgcgcgcgga ttgctcctcc tgccggaaag cggtctggaa catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMFQTAFRQE EQSARIHEKE VVDVFLQRRM MLTTASLVLQ LRMNGAPSAL NVPAPNACTP MAHNVRDDAI LRESCDYTVQ LLANSGQTPS VEMVTADGSV GYGYLFPTGD LSALRSSTPS ELVSAVLERY GKRGLDPLEA ARKKRILWFA VGRGGRGEEL HMIGASVVFK DERLYALVLT SVDLHSLVSP IERAGRVQQP VVVDSEGVPL VNADDAETVR KVDGRLAGQQ DGLYHWIPGF GWALRRPAPF SGFGHMTYLL PLDLQLRSMR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTCCAGACC GCTTTCCGGC AGGAGGAGCA ATCCGCGCGC ATCCACGAAA AGGAAGTGGT CGACGTGTTC CTGCAGCGCC GCATGATGTT GACGACGGCA AGCCTCGTGC TGCAACTGCG GATGAACGGC GCGCCTTCGG CGCTGAACGT GCCGGCGCCG AACGCGTGCA CGCCGATGGC CCACAATGTG CGCGACGATG CGATCCTGCG CGAGAGCTGC GATTACACGG TGCAGTTGCT GGCCAACTCG GGGCAGACGC CGAGCGTCGA AATGGTGACG GCCGACGGTT CGGTCGGCTA TGGATATCTG TTTCCGACGG GCGACCTGAG CGCGCTACGC TCCAGCACGC CGTCCGAACT CGTGTCGGCC GTGCTCGAGC GCTACGGCAA GCGCGGCCTG GACCCGCTGG AGGCCGCGCG CAAGAAGCGG ATTCTCTGGT TCGCGGTGGG CCGCGGCGGG CGCGGCGAGG AGCTGCATAT GATCGGCGCG TCAGTCGTGT TCAAGGACGA GCGGCTCTAC GCGCTCGTCT TGACGAGCGT GGATCTTCAC AGCCTCGTTT CGCCGATCGA GCGCGCCGGC CGCGTGCAGC AGCCGGTCGT CGTGGATTCC GAGGGCGTGC CGCTCGTGAA CGCGGACGAC GCGGAAACGG TCCGGAAGGT CGACGGGCGG CTCGCCGGAC AACAGGATGG CCTGTATCAC TGGATTCCCG GCTTCGGGTG GGCCCTACGC CGTCCCGCGC CGTTTTCCGG TTTCGGGCAC ATGACGTATC TGCTTCCGCT CGATCTGCAG TTGCGCTCGA TGCGCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA