BupsA.01664.d

Sensor protein (EC 2.7.13.3)

CENTER ID: BupsA.01664.d
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.01664.d.A1.GE34746 periplasmic domain 2CSK_N 39 171
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_171403
PATRIC ID: fig|320372.6.peg.204
RefSeq: YP_331625.1
UniProt: Q3JXS8
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPDPAVTSL RRTLLRRLAA PLSMLALMSG LIAYWLAWQY TQHVVDRSLA DLASAISKQI QIAGPDAPFT VPPLAQAMFS DPTEQLIYRI SDGEHELAGD PKLPLQGTSV RRMHVAYVFE AEYDNQAVRV AQVRVAQTES GNPMIVEVAQ PVRYRYRIAA EFLIAIMMPL LLLLLAGWGI VWRVVNQQLG PLTHLADSLN RQTHTSLEPV DETEVPLEIR PLTSAMNALL GRLKTALDAQ RKFIADAAHQ LRTPLTAIKL HAEQAAVARD PQQALTAVRE LRSAADRAVR LSNQLLSLAR AEPGEQAARF VDVDLAALAF ETGAEWVPRA LAAHVDLGFQ RGDGRGDDEP LLVRGNPVLL REVIANLLDN ALKYVPLARP QGARITMDVS RIALDEGHAA AEIVVEDNGS GVPAAQQADL FKRFFRGDAQ SGNGVETGAG LGLAIVHDII AMHGGTVSYA DAPEGGSCFI VRVPLADTPS TPAPPPCESP AASA
NT Sequence
atgtcccctg atccggctgt gaccagcctg cgccgcacgc tgctgcggcg tttagccgcg ccgctgtcga tgctcgcgct gatgagcggc ctcattgcct actggcttgc gtggcaatac acgcagcacg tcgtcgatcg ctcgcttgcc gatctcgcct cggcgatctc caagcagatc cagatcgccg gccccgacgc gccgttcacg gtgccgccgc tcgcacaggc gatgttctcc gatccgaccg agcaattgat ctaccggatc agcgacggcg aacacgagct cgccggcgat ccgaagctgc cgctgcaagg cacgagcgtg cgccggatgc atgtcgccta tgtattcgag gccgagtacg acaaccaggc ggtgcgcgtc gcgcaagtgc gcgtcgcgca gacggaaagc ggcaatccga tgatcgtcga ggtcgcgcag ccggtgcgct atcgttaccg gatcgcggcg gaattcctga tcgcgatcat gatgccgctg ctgctgctgc tgctcgccgg ctggggaatc gtgtggcgcg tcgtcaacca gcagctcggc ccgctcacgc atctcgccga ttcgctgaac cggcagacgc acacgtcgct cgagcccgtc gatgaaaccg aggtgccgct cgagatccgg ccgctcacga gcgcgatgaa tgcgctgctc ggccgcctga aaaccgcact cgacgcgcag cgcaagttca tcgccgatgc cgcgcatcag ctgcgcacgc cgctcaccgc gatcaagctg cacgcggagc aggcggccgt tgcgcgcgat ccgcaacagg cgctgaccgc ggtgcgcgag ctgcgctcgg cggccgatcg cgccgtgcgg ctgtcaaatc agttgctgtc gctcgcgcgc gcggagccgg gcgagcaggc ggcccgcttc gtcgatgtcg atctggccgc cctcgcattc gaaaccggtg ccgaatgggt gccgcgcgcg ctcgccgcgc atgtcgacct cggcttccaa cgcggcgacg gccgcggcga cgatgagccg ctcctcgtgc gcggcaaccc ggtgctgctg cgcgaagtga tcgcgaacct gctcgacaac gcgctgaaat acgtgccgct cgcgcggccg cagggcgcgc ggatcacgat ggacgtgtcg cggatcgcgc tcgacgaagg ccacgcggcg gccgagatcg tcgtcgagga caacggctcg ggcgtgcccg ccgcgcagca ggcggacctg ttcaagcgct tcttccgcgg cgacgcgcaa agcggcaacg gcgtcgagac gggcgcgggc ctcggcctcg cgatcgtcca cgacatcatc gcgatgcacg gcggaaccgt atcgtacgcg gatgcgccgg aaggcggctc gtgcttcatc gtacgcgtgc cgctcgcgga cacgccgtcc acgcccgcgc cgcccccctg tgaatcgccg gccgcttcgg cctga
Details for BupsA.01664.d.A1.GE34746
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 17kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQYTQHVVD RSLADLASAI SKQIQIAGPD APFTVPPLAQ AMFSDPTEQL IYRISDGEHE LAGDPKLPLQ GTSVRRMHVA YVFEAEYDNQ AVRVAQVRVA QTESGNPMIV EVAQPVRYRY RIAAEFLIAI MMPLL
Validated NT Sequence
annnnntttg tttactttaa gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg caatacacgc agcacgtcgt cgatcgctcg cttgccgatc tcgcctcggc gatctccaag cagatccaga tcgccggccc cgacgcgccg ttcacggtgc cgccgctcgc acaggcgatg ttctccgatc cgaccgagca attgatctac cggatcagcg acggcgaaca cgagctcgcc ggcgatccga agctgccgct gcaaggcacg agcgtgcgcc ggatgcatgt cgcctatgta ttcgaggccg agtacgacaa ccaggcggtg cgcgtcgcgc aagtgcgcgt cgcgcagacg gaaagcggca atccgatgat cgtcgaggtc gcgcagccgg tgcgctatcg ttaccggatc gcggcggaat tcctgatcgc gatcatgatg ccgctgctgt aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnanatn nnnnnnngnn nnnnnncnnc nntnnngnnn ngcnnggcac nncncnnnag nnnnnnnnnn nnnccnnnnn nnngnntnnn nnncncnnnn nnnnannggn gcttnncnnc ccngnncnac ncncncnnnc nnnagtgtga cagggcntcc nntnnannaa nannccccna cncncctnnn ntccnccgcn ctcnnnangc nnnnnnnncn ngnnnnnnnc cnnnnnnncc nagncncnnc nnnnnnnnnn cnncnnnnnn ccnngcnncc cnnnnnnnnn agnntnncnc ccncccnnna cnncngnncc nnnnnncnnn nnnnaaaanc nnnnnnnnnn nnnannannn nnngccnnnc gtnnnnnnnn nnncnnnann nnnnnnnnnn ncggggnnnn nnnnnnnnnn nnnaanannn ntnnnnccna ntnnncnnnn nc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQYTQHVVD RSLADLASAI SKQIQIAGPD APFTVPPLAQ AMFSDPTEQL IYRISDGEHE LAGDPKLPLQ GTSVRRMHVA YVFEAEYDNQ AVRVAQVRVA QTESGNPMIV EVAQPVRYRY RIAAEFLIAI MMPLL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaatacacg cagcacgtcg tcgatcgctc gcttgccgat ctcgcctcgg cgatctccaa gcagatccag atcgccggcc ccgacgcgcc gttcacggtg ccgccgctcg cacaggcgat gttctccgat ccgaccgagc aattgatcta ccggatcagc gacggcgaac acgagctcgc cggcgatccg aagctgccgc tgcaaggcac gagcgtgcgc cggatgcatg tcgcctatgt attcgaggcc gagtacgaca accaggcggt gcgcgtcgcg caagtgcgcg tcgcgcagac ggaaagcggc aatccgatga tcgtcgaggt cgcgcagccg gtgcgctatc gttaccggat cgcggcggaa ttcctgatcg cgatcatgat gccgctgctg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa