BupsA.01664.b

Sensor protein (EC 2.7.13.3)

CENTER ID: BupsA.01664.b
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.01664.b.A1.GE34747 periplasmic domain 2CSK_N 382 515
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_171049
PATRIC ID: fig|320372.6.peg.981
RefSeq: YP_332382.1
UniProt: Q3JVM1
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRECRHCIKK QWFMQARVAG RHRPISETAA RSVIAAQRQS VAHHANSHRR RRQHTSGRPH PVTPPIGLCR RSREERRRGR HGPVDADFRP ADPRSRPAQN VRPRGAAPPA RAQLEPARAD PDGRRQRRRA RERARPGRRR LHGEAVRAQR TRGARARADA ARRGRRADRR AAWLAVVRPG RPDRLCERTR ARAVRARARA ARGAAAAHRP AGVEGAARRS PVRMGRGSQQ QRDRGLRPPA AQEDRAERRA DHDRARPRLL PRESRARGRE RRAERRRVAG GRGRRRPAGA LAPPTRERPM ATPVRPAHAS RRASAAAPDT DAARDARYEN PFAPPDEADA AEGTRPRSLF GEILDWMLAP LLLLWPMSIA VTYLVAKTIA NGPFDRALET DAYVLARQIT PINGVAELRL PQATLDFLRA DNVDSLYFQV LGTRGELVAG EADLPLPRDD DRPAPGVVVF RDDLLRGNDV RVAYTSVALQ GATGSQPVLV QVGETLDKRN ALANDIIKGV ILPQFVILPL AILLVWFGLS RGLAPLTALQ AHIRGRRPDD LSPVEARRAP PEIEPLVTSF NDLLARLEQN ITLQKRFIAD AAHQMKTPLA GLRTQAEFAL RHEVNADVAR SLEQIATSSE QAARLVTQLL ALARAENRAT GLTFEPVEIA SLARQAVRDW VQAALAKQMD LGYEGPDTDA PLRIDGQPVM LREMLGNLID NAIRYTPAGG RITVRVHAER AAGAVHLEVE DTGPGIPPNE RERVVERFYR ILGREGDGSG LGLAIVREIV AQHGGTLTID DNVYQTSPRL AGTLVRVSIG LRQEAPE
NT Sequence
gtgcgcgaat gcagacactg tataaaaaaa cagtggttta tgcaagcccg cgttgcaggc aggcatcgcc caatttcgga gacagccgcg cgcagcgtca ttgccgccca gcggcaatcg gttgcgcatc atgcgaattc tcatcgccga agacgacagc atactagcgg acggcctcac ccggtcactc cgccaatcgg gctatgccgt cgatcacgtg aggaacggcg tcgaggccga cacggccctg tcgatgcaga ctttcgacct gctgatcctc gatctcggcc tgcccagaat gtccggcctc gaggtgctgc gccgcctgcg cgcgcgcaac tcgaacctgc ccgtgctgat cctgacggcc gccgacagcg tcgacgagcg cgtgaaaggg ctcgacctgg gcgccgacga ctacatggcg aagccgttcg cgctcaacga actcgaggcg cgcgtgcgcg cgctgacgcg gcgcggcgcg ggaggcgggc cgaccgtcgt gcggcatggc tcgctgtcgt tcgaccaggt cggccggatc gcctatgcga acgaacgcgt gctcgagctg tccgcgcgcg agctcgggct gctcgaggtg ctgctgcagc gcatcggccg gctggtgtcg aaggagcagc tcgtcgatca cctgtgcgaa tggggcgagg aagtcagcaa caacgcgatc gaggtctacg tccaccggct gcgcaagaag atcgagccga gcggcgtgcg gatcacgacc gtgcgcggcc tcggctactg cctcgagaaa gccgcgcccg cgggcgcgag cgacgcgccg aacgccgccg cgtcgccggc ggccgaggcc gtcgccggcc agccggtgcg ctagcgccgc ccacgcgcga gcggccgatg gccacgccgg ttcgtcccgc ccacgcctcg cgccgcgcga gcgccgcggc gcccgatacg gatgccgcgc gcgacgcgcg ctacgagaac ccgttcgcgc cgccggacga agccgacgcc gccgaaggca cccgcccgcg ctcgctgttc ggcgagattc tcgactggat gctcgcgccg ctccttctgc tgtggccgat gagcatcgcc gtcacctacc tcgtcgcgaa aacgatcgcg aacggcccgt tcgaccgcgc gctcgagacc gacgcttacg tgctcgcccg gcagatcacg cccatcaacg gcgtcgccga actgagactg ccgcaggcga cgctcgactt cctgcgcgcc gacaacgtcg atagcctcta tttccaggtg ctcggcacgc gcggcgaact cgtcgcgggc gaagccgacc tgccgctgcc gcgcgacgac gaccggccgg cgccgggcgt cgtcgtgttc cgcgacgatc tgctgcgcgg caacgacgtg cgcgtcgcat acacgagcgt cgcgctgcag ggcgcgacgg gctcgcagcc cgtgctcgtg caggtcggcg agacgctgga caagcgcaac gcgctcgcca acgacatcat caagggcgtg atcctgccgc aattcgtgat cctgccgctc gcgatcctgc tcgtctggtt cgggctgtcg cgcgggctcg cgccgctcac cgcgctgcag gcgcacattc gcggccggcg cccggacgat ctgtcgcccg tcgaggcgcg gcgcgcgccg ccagagatcg agccgctcgt cacgtcgttc aacgatctgc tcgcgcgtct cgaacagaac atcacgctgc aaaagcgctt catcgccgac gccgcgcacc agatgaaaac gccgctcgcc ggcctgcgca cgcaggcgga attcgcgctg cgccatgaag tgaacgccga cgtcgcacgc tcgctcgaac agatcgcgac gagctccgag caggcggcgc gactcgtcac gcaactgctc gcgctcgcgc gcgcggaaaa tcgcgcgacg gggctcacgt tcgagcccgt cgagatcgcg tcgctcgcgc ggcaggcggt gcgcgactgg gtgcaggccg cgctcgcgaa gcagatggat ctcggctacg aaggcccgga caccgacgcc ccgcttcgca tcgacggaca gccggtgatg ctgcgcgaga tgctcggcaa cctgatcgac aacgcgattc gctacacgcc ggcgggcggc cgcatcaccg tgcgcgtgca cgccgagcgc gcggcgggcg ccgtgcatct cgaagtcgag gacacgggcc ccggcattcc gccgaacgag cgcgagcggg tggtcgagcg cttctaccgg atcctcggcc gcgaaggcga cggcagcggg ctcggccttg cgatcgtccg cgagatcgtc gcgcaacacg gcggcacgct gacgatcgac gacaacgtct atcagacgtc gccgcgcctc gcgggcacgc tcgtgcgcgt gagcatcggc ttacggcaag aggccccgga ataa
Details for BupsA.01664.b.A1.GE34747
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 16kDa
OBSERVED MW: 17kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGPFDRALE TDAYVLARQI TPINGVAELR LPQATLDFLR ADNVDSLYFQ VLGTRGELVA GEADLPLPRD DDRPAPGVVV FRDDLLRGND VRVAYTSVAL QGATGSQPVL VQVGETLDKR NALANDIIKG VIL
Validated NT Sequence
cggcaggatc acgcccttga tgatgtcgtt ggcgagcgcg ttgcgcttgt ccagcgtctc gccgacctgc acgagcacgg gctgcgagcc cgtcgcgccc tgcagcgcga cgctcgtgta tgcgacgcgc acgtcgttgc cgcgcagcag atcgtcgcgg aacacgacga cgcccggcgc cggccggtcg tcgtcgcgcg gcagcggcag gtcggcttcg cccgcgacga gttcgccgcg cgtgccgagc acctggaaat agaggctatc gacgttgtcg gcgcgcagga agtcgagcgt cgcctgcggc agtctcagtt cggcgacgcc gttgatgggc gtgatctgcc gggcgagcac gtaagcgtcg gtctcgagcg cgcggtcgaa cgggcccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGPFDRALE TDAYVLARQI TPINGVAELR LPQATLDFLR ADNVDSLYFQ VLGTRGELVA GEADLPLPRD DDRPAPGVVV FRDDLLRGND VRVAYTSVAL QGATGSQPVL VQVGETLDKR NALANDIIKG VILPQF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcccgttc gaccgcgcgc tcgagaccga cgcttacgtg ctcgcccggc agatcacgcc catcaacggc gtcgccgaac tgagactgcc gcaggcgacg ctcgacttcc tgcgcgccga caacgtcgat agcctctatt tccaggtgct cggcacgcgc ggcgaactcg tcgcgggcga agccgacctg ccgctgccgc gcgacgacga ccggccggcg ccgggcgtcg tcgtgttccg cgacgatctg ctgcgcggca acgacgtgcg cgtcgcatac acgagcgtcg cgctgcaggg cgcgacgggc tcgcagcccg tgctcgtgca ggtcggcgag acgctggaca agcgcaacgc gctcgccaac gacatcatca agggcgtgat cctgccgcaa ttcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa