BupsA.01663.f

Hep_Hag family

CENTER ID: BupsA.01663.f
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.01663.f.A3.GE29413 head_YNlong domain 31 557
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_135314
PATRIC ID: fig|320372.6.peg.5720
RefSeq: YP_336752.1
UniProt: Q3JI51
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPRAALVTL LLAAWSAPSV AQALHPGSGA QSVEVGAGSN ASGSQSTALG NGAQATGSHS TATGQGSHAT GSNSTATGQD ANASGTSSTA TGQGSQATGS NSTATGQDAN ASGESSTATG QGSQATGDNS TATGQGATAS GESSTATGQG SQATGSNSTA TGQDANASGE SSTATGQGSQ ATGSNSTATG QGATASGESS TATGQGSQAT GDNSTATGQD ANASGESSTA MGQGSQATGS NSTATGQDAI ASGESSTATG QDSQATGDNS TATGQDANAS GESSTATGQG SQATGSNSTA TGQDATASGE SSTATGQGSQ ATGSNSTATG QDATASGESS TATGQGSQAT GSNSTATGED ANASGESSTA TGQGAQATGD NSTAAGQNAA ASGESSTATG QGSQAAGSNS TANGAGAAAT GHQSTALGAG AQAAGSQGVA SGWNANASGT QGVAIGGNAS AQGNQSIALG ANASATGDHS IALGAGSQAT GSNSVALGQG SIADRDNSVS VGSDGGERNV TNVANGWHDT DAVNFRQLRE VARYAYSGIA AATALAMIPD VDAGKTFSIG VGTGGYLGYQ AVAVGASARL GQNLKVRVGA GISAASTTWG AGASYSW
NT Sequence
atgacgccgc gagcggcact tgtcacgctt cttctcgcgg catggtctgc gccgagcgtt gcgcaagcac tccaccctgg atcgggggcg cagagtgttg aagtgggggc gggatcgaac gcatcgggtt cgcaaagcac cgcactgggt aacggtgcgc aggcgacggg cagtcatagc acggcaacgg gccaaggctc gcatgcgacg ggtagtaaca gcacggcaac gggtcaggat gctaacgcgt cgggcacatc gagcacggcg acgggccaag gttcgcaagc gacgggtagc aacagcacgg caacggggca ggatgctaac gcgtcgggtg aatcgagcac ggcgacgggc caaggttcgc aagcgacggg ggacaacagc acggcaacgg gccagggtgc taccgcgtcg ggtgaatcga gcacggcgac gggccagggt tcgcaagcga cgggtagcaa cagcacggca acggggcagg atgctaacgc gtcgggtgaa tcgagcacgg cgacgggcca aggctcacaa gcgacgggca gcaacagcac ggcaacgggg cagggtgcta ccgcgtcggg tgaatcgagc acggcgacag gccaaggctc gcaagcgacc ggggataaca gcacggcaac gggtcaagat gctaacgcgt cgggtgaatc gagcacggcg atgggccaag gttcgcaagc gacgggcagc aacagcacgg caacggggca ggatgctatc gcgtcgggtg aatcgagcac ggcgacaggc caagactcgc aagcgaccgg ggataacagc acggcaacgg gtcaggatgc taacgcgtcg ggggaatcga gcacggcgac gggccaaggt tcgcaagcga cgggcagcaa cagcacggca acggggcagg atgccaccgc gtcgggggaa tcgagcacgg cgacgggcca aggttcgcaa gcgacgggca gtaacagcac ggcaacgggg caggatgcca ccgcgtcggg ggaatcgagc acggcgacgg gccaaggttc gcaagcgacg ggcagcaaca gcacggcaac gggggaggat gctaacgcgt cgggtgaatc gagcacggcg acaggccaag gtgcgcaagc gacgggggac aacagcacgg cagcgggcca gaatgccgcc gcgtcgggtg aatcgagcac ggcgacgggc caaggctcgc aagcggcggg cagcaacagc acggcgaacg gcgccggcgc cgccgcgacc ggacaccaga gcactgctct gggcgcgggc gctcaagccg ctggctcaca gggcgtcgcg agcggctgga acgcaaacgc gtcggggact caaggcgtcg caatcggcgg caatgcctcg gcgcagggca atcaaagcat cgcgctcgga gccaatgcgt cggcgacggg cgaccacagc atcgcgttgg gcgccggctc gcaggcaacg ggctcgaact cggtcgcgct gggccagggg tcgatcgcgg accgcgacaa ctcggtttcg gtgggttcgg atggcggtga gcgcaacgtc accaatgtgg cgaatggatg gcacgatacc gacgccgtca atttccggca actgcgcgag gtcgcgcgct acgcatacag cggcatcgct gccgcaactg cgctggccat gattccggac gtcgacgcag gcaagacgtt ttcgatcggc gtcgggacgg gcggctacct cggctatcag gctgttgccg tcggcgcgtc ggctcgcctt ggacaaaatc tgaaggtccg ggtgggcgcg ggcattagcg cggcgtcgac gacatggggc gccggcgcat cgtatagctg gtag
Details for BupsA.01663.f.A3.GE29413
HARVESTED ON: 5/27/2010
SEQUENCED ON: 6/14/2010
EXPECTED MW: 50kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQSVEVGAG SNASGSQSTA LGNGAQATGS HSTATGQGSH ATGSNSTATG QDANASGTSS TATGQGSQAT GSNSTATGQD ANASGESSTA TGQGSQATGD NSTATGQGAT ASGESSTATG QGSQATGSNS TATGQDANAS GESSTATGQG SQATGSNSTA TGQGATASGE SSTATGQGSQ ATGDNSTATG QDANASGESS TAMGQGSQAT GSNSTATGQD AIASGESSTA TGQDSQATGD NSTATGQDAN ASGESSTATG QGSQATGSNS TATGQDATAS GESSTATGQG SQATGSNSTA TGQDATASGE SSTATGQGSQ ATGSNSTATG EDANASGESS TATGQGAQAT GDNSTAAGQN AAASGESSTA TGQGSQAAGS NSTANGAGAA ATGHQSTALG AGAQAAGSQG VASGWNANAS GTQGVAIGGN ASAQGNQSIA LGANASATGD HSIALGAGSQ ATGSNSVALG QGSIADRDNS VSVGSDGGER NVTNVANGWH DTDAVNFRQL REVARYAYSG IAAATALAM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcagagtgtt gaagtggggg cgggatcgaa cgcatcgggt tcgcaaagca ccgcactggg taacggtgcg caggcgacgg gcagtcatag cacggcaacg ggccaaggct cgcatgcgac gggtagtaac agcacggcaa cgggtcagga tgctaacgcg tcgggcacat cgagcacggc gacgggccaa ggttcgcaag cgacgggtag caacagcacg gcaacggggc aggatgctaa cgcgtcgggt gaatcgagca cggcgacggg ccaaggttcg caagcgacgg gggacaacag cacggcaacg ggccagggtg ctaccgcgtc gggtgaatcg agcacggcga cgggccaggg ttcgcaagcg acgggtagca acagcacggc aacggggcag gatgctaacg cgtcgggtga atcgagcacg gcgacgggcc aaggctcaca agcgacgggc agcaacagca cggcaacggg gcagggtgct accgcgtcgg gtgaatcgag cacggcgaca ggccaaggct cgcaagcgac cggggataac agcacggcaa cgggtcaaga tgctaacgcg tcgggtgaat cgagcacggc gatgggccaa ggttcgcaag cgacgggcag caacagcacg gcaacggggc aggatgctat cgcgtcgggt gaatcgagca cggcgacagg ccaagactcg caagcgaccg gggataacag cacggcaacg ggtcaggatg ctaacgcgtc gggggaatcg agcacggcga cgggccaagg ttcgcaagcg acgggcagca acagcacggc aacggggcag gatgccaccg cgtcggggga atcgagcacg gcgacgggcc aaggttcgca agcgacgggc agtaacagca cggcaacggg gcaggatgcc accgcgtcgg gggaatcgag cacggcgacg ggccaaggtt cgcaagcgac gggcagcaac agcacggcaa cgggggagga tgctaacgcg tcgggtgaat cgagcacggc gacaggccaa ggtgcgcaag cgacggggga caacagcacg gcagcgggcc agaatgccgc cgcgtcgggt gaatcgagca cggcgacggg ccaaggctcg caagcggcgg gcagcaacag cacggcgaac ggcgccggcg ccgccgcgac cggacaccag agcactgctc tgggcgcggg cgctcaagcc gctggctcac agggcgtcgc gagcggctgg aacgcaaacg cgtcggggac tcaaggcgtc gcaatcggcg gcaatgcctc ggcgcagggc aatcaaagca tcgcgctcgg agccaatgcg tcggcgacgg gcgaccacag catcgcgttg ggcgccggct cgcaggcaac gggctcgaac tcggtcgcgc tgggccaggg gtcgatcgcg gaccgcgaca actcggtttc ggtgggttcg gatggcggtg agcgcaacgt caccaatgtg gcgaatggat ggcacgatac cgacgccgtc aatttccggc aactgcgcga ggtcgcgcgc tacgcataca gcggcatcgc tgccgcaact gcgctggcca tgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa