BupsA.01663.e

Hep_Hag family

CENTER ID: BupsA.01663.e
ORGANISM: Burkholderia pseudomallei 1710b
ASSOCIATED DISEASE: Melioidosis
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: True
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BupsA.01663.e.A4.GE29410 head_YNlong domain 444 986
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_168109
PATRIC ID: fig|320372.6.peg.2326
RefSeq: YP_333623.1
UniProt: Q3JS30
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRIFKSIWC EQTRTWVAAS EHAVARGGRA SSVVASAGGL EKVLKLSILG AASLIAMGVV GPFAEEAMAA NNAGVCLTYN GSSNNTSGTG GWFADGCKSA GWVQGMVTNS KTDWVGLTAD DTQIVLDGSA GSIYFRTGGI NGNVLTMSNA TGGVLLSGLA AGVNPTDAVN MSQLTSLSTS TATGITSLST STATSIASLS TSMLSLGVGV VTQDASTGAI SVGANSPGLT VDFAGGQGAR TLTGVAAGVN ATDAVNVGQL ASLSTSTAAG LSTAASGVAS LSTSLLGAAG DLASLSTSAS TGLATADSGI ASLSTSLLGT ADNVTSLSTS LSTVNANLAG LQTSVDNVVS YDDPSKSAIT LGGAGVTTPV LLTNVAAGKI AATSTDAVNG SQLYTLQQEF SQQYDLLTSQ VSSLSTSVSG LQGSVSANTG TASGDNSTAS GDNATASGTN STANGTNSTA SGDNSTASGT NASASGENST ATGTDSTASG SNSTANGTNS TASGDNSTAS GTNASATGEN STASGTNASA TGENSTATGT ASTASGSNST ANGTNSTASG ENSTATGTDS TASGSNSTAN GTNSTASGDN STASGTNASA TGENSTATGT DSTASGSNST ANGTNSTASG DNSTASGTNA SATGENSTAT GTDSTASGSN STANGANSTA SGDNSTASGT NASATGENST ATGTDSTASG SNSTANGTNS TASGNNSTAS GTNASATGEN STATGTDSAA SGTNSTANGT NSTASGDNST ASGTNASATG ENSTATGTAS TASGSNSTAN GANSTASGAG ATATGENAAA TGAGATATGN NASASGTSST AGGANAIASG ENSTANGANS TASGNGSSAF GESAAAAGDG STALGSNAVA SGVGSVATGA GSVASGANSS AYGTGSNATG AGSVAIGQGA TASGSNSVAL GTGSVASEDN TVSVGSAGSE RRITNVAAGV NATDAVNVGQ LNSAVSGIRN QMDGMQGQID TLARDAYSGI AAATALTMIP DVDPGKTLAV GIGTANFKGY QASALGATAR ITQNLKVKTG VSYSGSNYVW GAGMSYQW
NT Sequence
atgaacagga ttttcaaatc gatctggtgc gaacagacgc gtacgtgggt tgcggcatcg gagcatgccg tggcgcgcgg tggccgcgcg tcgagcgtcg tcgcgtccgc cggcggattg gagaaggtgc tcaagctgtc gattctgggc gcggcatcgc tgattgcgat gggcgtggtc ggaccgtttg ccgaggaggc aatggcggcg aataacgccg gtgtgtgttt gacgtacaac ggtagtagca acaatacatc aggtactggc ggctggttcg ctgatggttg taaatcggcc ggctgggtgc agggcatggt tacgaatagc aagacggatt gggtcgggct gaccgcggac gacacgcaga tcgtgctcga cggtagcgcg ggcagcattt acttccggac gggcggcata aacggcaacg tgttgacgat gtcgaacgcg accggcggcg tattgctcag cggcctcgcg gccggcgtca atccgaccga tgcggtcaac atgtcccagt tgacctcgtt gtcgacgtcg acggcaaccg gcatcacctc gctttcgacg tcgacggcaa ccagcatcgc ttcgctttcg acgagcatgc tgtcgctcgg cgtgggcgtc gtgacgcaag acgcctcgac cggcgcgatc agcgtcggcg ccaattcgcc gggcctgacg gtggatttcg cggggggcca gggcgcgcgc acgctgacgg gcgtcgccgc gggcgtcaac gctacggacg cggtcaatgt cggccagctg gcgtcgctgt cgacgagcac ggcagcgggg ctttccaccg ccgcgagcgg cgtcgcgtcg ctgtcgacgt cgctgctcgg cgcggcgggc gatctggcgt cactgtcgac gagcgcgtcg acggggctcg ccactgcgga tagcggcatc gcgtcgttgt ccacgtcgct gctcggtacc gcggacaacg tgacgtcgct gtcgacgagc ctcagcacgg tcaacgcgaa tctggccggc ctgcagacct cggtggacaa cgtcgtgtca tacgacgatc cgtcgaagtc ggcgatcacc ctcggcggcg cgggcgtcac gacgcccgtc ctgctgacga acgtggctgc ggggaagatc gccgcgacca gcacggacgc ggtgaacggt tcgcagcttt acacgctcca gcaggaattc tcgcagcagt acgatctgct gacgtcgcaa gtctcgtcgc tcagcacttc ggtgtcgggt ctccaaggca gcgtctcggc aaatacggga accgcgtcgg gtgacaacag cacggcgagc ggtgacaacg cgaccgcgtc gggcacgaac agcacggcca acggcacgaa ctcgaccgcg tcgggtgata acagcacggc aagcgggacg aatgcgtcgg cgagcggcga gaacagcacg gcgacgggta cggactcgac cgcatcgggt agcaatagca cggccaacgg gacgaactcg actgcgtcgg gtgataacag cacggcaagc gggacgaatg cgtcggcgac gggcgagaac agcacggcga gcggcacgaa cgcatcggcg acgggcgaga acagcacggc gacgggcacg gcttcgaccg catcgggcag taacagcacg gcgaacggca cgaactcgac cgcgtcgggc gagaacagca cggcgacggg tacggactcg accgcatcgg gcagcaacag cacggccaac gggacgaact cgaccgcgtc gggtgataac agcacggcga gcggcacgaa cgcatcggcg accggtgaga acagcacggc gacaggcacg gattcgaccg catcgggcag caacagcaca gccaacggca cgaactcgac cgcgtcgggc gataacagca cggcgagcgg cacgaacgca tcagcgaccg gtgagaacag cacggcgacg ggtacggact cgaccgcatc gggcagcaac agcacggcca acggggcgaa ctcgaccgca tcgggcgata acagcacggc gagcggcacg aacgcatcgg cgacgggcga gaacagcacg gcgacaggca cggattcgac cgcatcgggc agcaacagca cggccaacgg aacgaactcg actgcgtcgg gcaacaacag cacggcgagc gggacgaacg cgtcggcgac gggtgaaaac agcacggcga cgggtacgga ctcggccgca tccggtacga atagcacggc caacggcacg aactcgaccg cgtcgggcga taacagcacg gcgagcggga cgaatgcgtc ggcgaccggt gagaacagca cggcgacggg tacggcttcg accgcgtcgg gcagcaacag cacggccaac ggtgcgaact cgacggcatc cggcgcgggg gcgacggcaa cgggtgaaaa cgccgcagcc acgggcgcgg gcgcgacggc gaccggcaac aacgcatcgg catcgggcac gagcagcacg gccggcggtg cgaatgcaat cgcgtcgggc gagaacagca cggccaacgg cgcgaactcg accgcatccg gcaacggcag ctcggcgttc ggcgagagcg cggcggcagc cggcgacggc agcacggcgc tgggttcaaa cgctgtcgcg tcgggtgtcg gcagcgtcgc gacgggcgcg ggttcggtcg cgtccggcgc gaacagttcg gcgtacggta cgggctcgaa cgcgacgggc gcgggcagcg tcgccatcgg tcagggcgcg acggcctcgg gatcgaactc ggtcgcgctt ggcaccggtt ctgtcgcgtc ggaggacaac acggtatcgg tcggctccgc aggcagcgag cgcaggatca ccaacgtcgc cgccggcgtc aatgccaccg acgccgtcaa cgtcggccag ttgaacagcg ccgtgtcggg catccggaat cagatggacg gcatgcaagg ccagatcgat acgcttgcac gcgatgcgta ttccggtatc gcggccgcga ccgcgttgac gatgattccg gacgtggatc cgggcaagac gctggccgtg ggcatcggca cggccaattt caagggctac caagcctccg cgctcggcgc gaccgcacgt atcacccaga acctcaaggt gaagacgggc gtgagctaca gcggcagcaa ctacgtgtgg ggcgcgggca tgtcgtatca atggtaa
Details for BupsA.01663.e.A4.GE29410
HARVESTED ON: data unavailable
SEQUENCED ON: 6/14/2010
EXPECTED MW: 50kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATASGTNS TANGTNSTAS GDNSTASGTN ASASGENSTA TGTDSTASGS NSTANGTNST ASGDNSTASG TNASATGENS TASGTNASAT GENSTATGTA STASGSNSTA NGTNSTASGE NSTATGTDST ASGSNSTANG TNSTASGDNS TASGTNASAT GENSTATGTD STASGSNSTA NGTNSTASGD NSTASGTNAS ATGENSTATG TDSTASGSNS TANGANSTAS GDNSTASGTN ASATGENSTA TGTDSTASGS NSTANGTNST ASGNNSTASG TNASATGENS TATGTDSAAS GTNSTANGTN STASGDNSTA SGTNASATGE NSTATGTAST ASGSNSTANG ANSTASGAGA TATGENAAAT GAGATATGNN ASASGTSSTA GGANAIASGE NSTANGANST ASGNGSSAFG ESAAAAGDGS TALGSNAVAS GVGSVATGAG SVASGANSSA YGTGSNATGA GSVAIGQGAT ASGSNSVALG TGSVASEDNT VSVGSAGSER RITNVAAGVN ATDAVNVGQL NSAVSGIRNQ MDGMQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgaccgcg tcgggcacga acagcacggc caacggcacg aactcgaccg cgtcgggtga taacagcacg gcaagcggga cgaatgcgtc ggcgagcggc gagaacagca cggcgacggg tacggactcg accgcatcgg gtagcaatag cacggccaac gggacgaact cgactgcgtc gggtgataac agcacggcaa gcgggacgaa tgcgtcggcg acgggcgaga acagcacggc gagcggcacg aacgcatcgg cgacgggcga gaacagcacg gcgacgggca cggcttcgac cgcatcgggc agtaacagca cggcgaacgg cacgaactcg accgcgtcgg gcgagaacag cacggcgacg ggtacggact cgaccgcatc gggcagcaac agcacggcca acgggacgaa ctcgaccgcg tcgggtgata acagcacggc gagcggcacg aacgcatcgg cgaccggtga gaacagcacg gcgacaggca cggattcgac cgcatcgggc agcaacagca cagccaacgg cacgaactcg accgcgtcgg gcgataacag cacggcgagc ggcacgaacg catcagcgac cggtgagaac agcacggcga cgggtacgga ctcgaccgca tcgggcagca acagcacggc caacggggcg aactcgaccg catcgggcga taacagcacg gcgagcggca cgaacgcatc ggcgacgggc gagaacagca cggcgacagg cacggattcg accgcatcgg gcagcaacag cacggccaac ggaacgaact cgactgcgtc gggcaacaac agcacggcga gcgggacgaa cgcgtcggcg acgggtgaaa acagcacggc gacgggtacg gactcggccg catccggtac gaatagcacg gccaacggca cgaactcgac cgcgtcgggc gataacagca cggcgagcgg gacgaatgcg tcggcgaccg gtgagaacag cacggcgacg ggtacggctt cgaccgcgtc gggcagcaac agcacggcca acggtgcgaa ctcgacggca tccggcgcgg gggcgacggc aacgggtgaa aacgccgcag ccacgggcgc gggcgcgacg gcgaccggca acaacgcatc ggcatcgggc acgagcagca cggccggcgg tgcgaatgca atcgcgtcgg gcgagaacag cacggccaac ggcgcgaact cgaccgcatc cggcaacggc agctcggcgt tcggcgagag cgcggcggca gccggcgacg gcagcacggc gctgggttca aacgctgtcg cgtcgggtgt cggcagcgtc gcgacgggcg cgggttcggt cgcgtccggc gcgaacagtt cggcgtacgg tacgggctcg aacgcgacgg gcgcgggcag cgtcgccatc ggtcagggcg cgacggcctc gggatcgaac tcggtcgcgc ttggcaccgg ttctgtcgcg tcggaggaca acacggtatc ggtcggctcc gcaggcagcg agcgcaggat caccaacgtc gccgccggcg tcaatgccac cgacgccgtc aacgtcggcc agttgaacag cgccgtgtcg ggcatccgga atcagatgga cggcatgcaa aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa