BupsA.01663.c
Hep_Hag family
| CENTER ID: | BupsA.01663.c |
| ORGANISM: | Burkholderia pseudomallei 1710b |
| ASSOCIATED DISEASE: | Melioidosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | True |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.01663.c.A1.GE26295 | BupsA.01663.c.1 (Hep_Hag HIM 1) | 314 | 501 | ||
| BupsA.01663.c.A2.GE26296 | BupsA.01663.c.2 (Hep_Hag HIM 2) | 1131 | 1304 | ||
| BupsA.01663.c.A8.GE29407 | head2_HaYHN domain | 1057 | 1315 | ||
| BupsA.01663.c.A9.GE29408 | head3_YN domain | 1381 | 1553 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BupsA.01663.c.A1.PS00198b | BupsA.01663.c.1 (Hep_Hag HIM 1) | 314 | 501 | ||
| BupsA.01663.c.A2.PS00264 | BupsA.01663.c.2 (Hep_Hag HIM 2) | 1131 | 1304 |
Structures
3S6L
DEPOSITED: 5/25/2011
DETERMINATION: XRay
CLONE: BupsA.01663.c.A9.GE29408
PROTEIN: BupsA.01663.c.A9.PW29322
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126579 |
| PATRIC ID: | fig|320372.6.peg.6579 |
| RefSeq: | YP_337531.1 |
| UniProt: | Q3JFX2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLMFSTPAWA ALVGAENGTV NSSDGTASTT AGSGSAQASN TGIAFVGDVD CSAFTSGPGT YAGVYGSGSN YLGGLFGFGP QTSMASYGDP SNAGANAGIV AWQGAAQTFG NVTYAGNGTQ SGSHTQAFGM NSFAAGCVAH ATGLSATAIG WGTTASGAGS VALGLYSTAS GQGSLAFGTS ATATATDTIA LGTLATANAV SGVAIGANTQ ASAANATAIG GNSSGANLGA QATAAGATAI GGNATAGAAA TATNAIAIGG QSSAKDANDV AVGLGARAGT GSGAGNDLAI GNGATATGGN SIAQGAGASA NAAGAVAIGK SASAAGGQAV SIGVANTASG NGAVAIGDPN VATGTGAVAL GNNNTANGQG AVALGNVSTA VGQGSVALGN SSNAAAAGGV ALGDTASAVM AGGLALGSLA TASNANDVAL GAGSKTAAAV ATSTVSVNGA NYAVAGSGPA STVSVGAPGS ERTITNVAAG QVSAGSTDAV NGSELYATNQ AITTGLSTAN SSIASLSTST STGLSSANSN IGSLSTGLST TNSTVASLST STVAGLNSLS TGLSTTNSNV ASLSSSTSTG LSSANSAVAS LSTSTSTGLS SANSNIDSLS TGLSTTNSTV ASLSTSTVAG LNSLSTGLST TNSNVASLSS STSTGLSSAN SAVASLSTST STGLSSANSN IGSLSTGLST ANSTVASLST STVAGLNSLS TGLSTTNSNV ASLSSSTSTG LSSANSAVVS LSTSASTGLS SANSNIGSLS TGLSTTNSTV ASLSSSTSTG IGSLSTGVAN SVQYDSPAHT SITLGGASAT SPVKITNLAA GANPSDAVNY EQLTSLSTSA STGLSSANSA ITSLSTSTST GIGSLSTGLS TTNSNVASLS TSASTGLSSA NSAITSLSTS TSTGIGSLST GLSTTNSNVA SLSTSASTGL SSANSAITSL STSTSTGIGS LSTGLSTTNS NVASLSTSAS TGLSSAISAI TSLSTSTSTG IGSLSTGLST TNSNVASLST SASTGLSSAN SAITSLSTST STGIGSLSTG LSTTNSNLSS LSTSSSTGLS TANSNISSLS TGLNSLSTAV NGGGTKYFHA NSTQPDSQAL GADSVAVGPA AIAAGASGIA IGNAANAAAN GAVAIGQAAV AKGGLAVSIG VSNTASGDGA VAIGDPNVAT GTGAVALGAD NSANGQGAVA LGNANIATGT GSLAFGNTST AAAAGAVALG AGAIANNAND VALGSASVTA AANPVASALI AGQAYSLAGG APASVVSVGA PGAERQITNV AAGRISATST DAVNGSQMNA MTQALESLST STASALSTAQ SGLGSLSTGL SSTQSSVSSL STGLSTTSGN VASLSSGLGT MQSGIASLST GLSTTNSSLA SLSTAVSGGG VRTSSLGDTS AGNGANASGG NGTAVGGAAS ASGTDATALG QASNASGNHS TALGQASSAS GSGSTAVGQG AGAPGDGASA FGQGALASGT DSTALGAHST AAAPNSAAIG ANSVASAPNS VSFGSRGHER RLTNVAPGID GTDAANMNQL WGVQSSVDQA ARRAYSGVAA ATALTMIPEV DPGKTIAVGI GAGSYQGYSA SAIGVSVRFS DNLKAKLGVG ISAQGSTYGA GVSYQW
NT Sequence
atgttgatgt tttccactcc cgcatgggct gccctcgtag gggctgaaaa tgggaccgtc aactcgtctg acgggacagc ctcgaccaca gccggcagtg gttccgctca agccagtaat acgggtattg ccttcgtcgg tgatgttgac tgctcggcgt ttacgtcggg acctggcaca tatgccggcg tgtacggctc cgggagcaat tatctgggtg gccttttcgg ctttggtccg caaacatcga tggccagtta cggtgatccg agcaacgccg gcgctaatgc cggtatcgtt gcatggcagg gcgctgccca aacgttcggc aatgtcacct acgccggcaa tggcacgcag agcggcagcc acacgcaggc gttcggcatg aactccttcg cggcgggctg cgttgctcac gcgaccggcc tgagcgcgac ggcgatcggc tggggaacca ccgcgagcgg cgccggaagc gtcgcgctcg ggctctacag caccgcgagc ggccagggat cgttggcgtt cggcaccagc gcgacggcga cggccaccga caccatcgcg ctcggcacgt tggccacggc caacgcggtc agcggcgtgg cgatcggcgc gaacacgcag gcttcggccg ccaatgcaac cgcgatcggc ggcaattcat ccggcgccaa cctcggcgcg caggcgacgg cggccggcgc cactgcgatc ggcggcaacg cgacggccgg ggccgccgcg acggcgacga acgccatcgc gatcggcggg cagtcgtcgg cgaaggatgc gaacgatgtg gccgtgggcc tgggcgcgag ggccggcacg ggaagcggcg cgggcaacga tctcgcgatc ggcaatggcg cgacggccac gggcggcaat tcgatcgccc agggcgcggg cgcgagcgcc aatgcggccg gcgcggtggc catcggcaaa tcggcgtccg ccgccggcgg gcaagccgtt tcgatcggcg tggccaatac cgcgtcgggc aacggcgcgg tggcgatcgg cgatccgaac gtcgcgaccg gaaccggcgc tgtcgcgctg ggcaacaaca atacggccaa tggtcaaggc gcggtggcgc tgggcaacgt cagcacggcg gtcggccaag gcagcgtggc gctcggcaac agcagcaatg cggccgcggc gggcggagtg gccttgggcg ataccgcgag cgcggtgatg gcgggcggct tggcactcgg ctcgctcgcg acggcgagca atgcgaacga cgtggcgctc ggcgcgggtt cgaaaaccgc cgcggcagtc gcgacgtcca cggtttcggt gaacggcgcc aactacgcgg tggcgggaag cggcccggcc agcacggtca gcgtgggtgc gccgggcagc gagcgcacga tcaccaatgt ggccgcgggt caagtaagcg ccggttccac cgatgcggtg aatggttcgg aactgtacgc gacgaaccag gcaatcacga ccggattgtc gacagcgaac agcagcatcg cgtcgctgtc cacgtcgacg tcgacgggtc tttcgagcgc caacagcaac atcggctcgt tgtcgacggg tttgtcgacc acgaacagca cggttgcgtc gttgtcgacg tccacggttg ccggcctgaa ttcgctgtcc accggattga gcacgaccaa tagcaatgtc gcgtcgctgt cgagttccac gtcgacggga ttgtcctcgg ccaatagcgc ggtggcgtca ctgtccacgt cgacgtcgac gggtctttcg agcgccaaca gcaacatcga ctcgttgtcg acgggcttgt cgaccacgaa cagcacggtt gcgtcgttgt cgacgtccac ggttgccggc ctgaattcgc tgtccaccgg attgagcacg accaatagca atgtcgcgtc gctgtcgagt tccacgtcga cggggctgtc ctcggccaat agcgcggtgg cgtcgctgtc cacgtcgacg tcgacgggtc tttcgagcgc caacagcaac atcggctcgt tgtcgacggg cttgtcgacc gccaacagca cggttgcgtc gttgtcgacg tccacggttg ccggcctgaa ttcgctgtcc accggattga gcacgaccaa tagcaatgtc gcgtcgctgt cgagttccac gtcgacggga ttgtcctcgg ccaatagcgc ggtggtgtcg ctgtccacgt cggcgtcgac gggtctctcg agcgcaaaca gcaacatcgg ctcgttgtcg acaggtttgt cgaccacgaa cagcacggtt gcgtcgttgt cgagctcgac gtcgaccggt atcggttcgt tgtcgacggg ggtggccaat tctgtccagt atgacagtcc tgctcatacg tccattaccc tgggtggcgc cagtgcaacg tcacccgtga agatcactaa tttggcggcg ggcgcgaacc cgagcgatgc cgtcaactat gagcaactga catcgctgtc gacgtcggcg tcgacgggac tgtcgtcggc caacagcgcg atcacgtcgc tgtccacctc gacgtcgacc ggcatcggct cgctgtccac cggactgagc acgaccaaca gcaacgtcgc gtcgttgtcg acgtcggcgt cgacgggact gtcgtcggcc aacagcgcga tcacgtcgct gtccacctcg acgtcgaccg gcatcggttc gctgtccacc gggttgagca cgaccaacag caacgtcgcg tcgctgtcga cgtcggcgtc gacgggactg tcgtcggcca acagcgcgat cacatcgcta tccacctcga cgtcgaccgg catcggttcg ctgtccaccg gactgagcac gaccaacagc aacgtcgcgt cgctgtcgac gtcggcgtcg acgggactgt cgtcggccat cagcgcgatc acgtcgctgt ccacctcgac gtcgaccggt atcggctcgc tgtccaccgg gttgagcacg accaacagca acgtcgcgtc gctgtcgacg tcggcgtcga cgggactgtc gtcggccaac agcgcgatca cgtcgctgtc cacctcgacg tcgaccggca tcggttcgct gtccaccggg ttgagcacga ccaacagcaa tctgagctcc ctgtccacgt cgagctcgac cggcttgagt acggccaaca gcaacatctc gtcgctgtcc accgggctga attcgttgtc gaccgcggtc aacggcggcg ggacgaagta cttccacgcc aactcgacgc agccggacag tcaggcgctg ggggcggatt ccgtcgcggt cgggccggcg gccatcgcgg cgggcgcaag cggcattgcg atcggcaatg cggcgaacgc ggccgcaaac ggcgccgtcg cgatcggcca ggccgccgtc gcgaagggcg ggctggctgt ctcgatcggg gtgtcgaaca cggcgagcgg agacggcgcg gtggcgatcg gcgatccgaa cgtcgcgacc ggcaccggcg cggtcgcgct tggcgcggac aattcggcaa acggccaggg cgccgtcgcg ctcggcaacg cgaacatcgc aaccggaacg ggctcgcttg cgttcggcaa cacgtcgacg gcggcagcgg cgggcgcggt cgcgttgggc gccggcgcaa tcgcgaacaa tgcgaacgat gtcgcgctgg gttccgctag cgtgaccgcg gctgcgaatc cggtggccag cgcgttgatc gcaggtcagg cttattcgct tgccggcggc gcgccggcga gcgtggtgag cgtcggcgcg cccggcgccg aacggcaaat caccaacgtc gcggccgggc ggatttccgc cacgtcgacc gatgcggtga acggctcgca gatgaatgcg atgactcagg cgctggaatc gctgtcgact tcgacggcca gcgcgctgtc cacggcgcaa agcggtctgg gttcgttgtc gacggggctc agctcgacgc agagcagcgt gagttcgctg tcgacggggc tcagcacgac gagcggcaat gtggcgtcgc tgtcgagcgg tctgggcacg atgcaaagcg gtatcgcgtc gctgtccacg gggctgagca cgacgaacag cagcctcgcg tcgctgtcga ccgccgtgtc cggcggcggt gttcgcacca gcagcttggg cgacacgtcg gcgggcaatg gcgcgaacgc gtccggcggc aacggcacgg cggtcggcgg cgccgcgtcc gcttcgggaa ccgatgcgac cgcgctgggc caggcgtcga acgcgtcggg caatcattcg accgcattgg ggcaagcatc gagcgcgtcc ggaagcggct ccaccgcggt gggacaaggc gccggcgcgc ccggcgacgg cgcttcggca ttcggccaag gggcacttgc ctccggtacg gactcgacgg cgctcggcgc tcattcgacg gctgcggcgc cgaactcggc ggcgatcggc gcgaattcgg tggcgtccgc gccgaattcg gtgtcgttcg gttcgcgggg ccatgagcgc aggctgacga atgtcgcgcc ggggatcgac ggcaccgacg cggcgaacat gaaccagctc tggggcgtgc aatcgagcgt cgatcaggcg gcgcgccgcg cctattccgg ggtggcggcc gcgaccgcgc tgacgatgat tccggaggtc gaccccggca agacgatcgc ggtcgggatc ggcgcgggca gctatcaagg gtattcggcg tccgcgatcg gcgtgtccgt gcggttctcc gacaacctga aggcgaagct cggcgtgggg atcagcgctc agggcagcac atatggcgca ggtgtctcgt accagtggta g
Details for BupsA.01663.c.A1.GE26295
| HARVESTED ON: | 3/10/2009 |
| SEQUENCED ON: | 3/26/2009 |
| EXPECTED MW: | 19kDa |
| OBSERVED MW: | 23kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MGTLEAQTQG PGSMGAVAIG KSASAAGGQA VSIGVANTAS GNGAVAIGDP NVATGTGAVA LGNNNTANGQ GAVALGNVST AVGQGSVALG NSSNAAAAGG VALGDTASAV MAGGLALGSL ATASNANDVA LGAGSKTAAA VATSTVSVNG ANYAVAGSGP ASTVSVGAPG SERTITNVAA GQVSAGSTDA VNGSELYAT
Validated NT Sequence
tcgtcgcgta cagttccgaa ccattcaccg catcggtgga accggcgctt acttgacccg cggccacatt ggtgatcgtg cgctcgctgc ccggcgcacc cacgctgacc gtgctggccg ggccgcttcc cgccaccgcg tagttggcgc cgttcaccga aaccgtggac gtcgcgactg ccgcggcggt tttcgaaccc gcgccgagcg ccacgtcgtt cgcattgctc gccgtcgcga gcgagccgag tgccaagccg cccgccatca ccgcgctcgc ggtatcgccc aaggccactc cgcccgccgc ggccgcattg ctgctgttgc cgagcgccac gctgccttgg ccgaccgccg tgctgacgtt gcccagcgcc accgcgcctt gaccattggc cgtattgttg ttgcccagcg cgacagcgcc ggttccggtc gcgacgttcg gatcgccgat cgccaccgcg ccgttgcccg acgcggtatt ggccacgccg atcgaaacgg cttgcccgcc ggcggcggac gccgatttgc cgatggccac cgcgcccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAVAIGKS ASAAGGQAVS IGVANTASGN GAVAIGDPNV ATGTGAVALG NNNTANGQGA VALGNVSTAV GQGSVALGNS SNAAAAGGVA LGDTASAVMA GGLALGSLAT ASNANDVALG AGSKTAAAVA TSTVSVNGAN YAVAGSGPAS TVSVGAPGSE RTITNVAAGQ VSAGSTDAVN GSELYATNQA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgcggtg gccatcggca aatcggcgtc cgccgccggc gggcaagccg tttcgatcgg cgtggccaat accgcgtcgg gcaacggcgc ggtggcgatc ggcgatccga acgtcgcgac cggaaccggc gctgtcgcgc tgggcaacaa caatacggcc aatggtcaag gcgcggtggc gctgggcaac gtcagcacgg cggtcggcca aggcagcgtg gcgctcggca acagcagcaa tgcggccgcg gcgggcggag tggccttggg cgataccgcg agcgcggtga tggcgggcgg cttggcactc ggctcgctcg cgacggcgag caatgcgaac gacgtggcgc tcggcgcggg ttcgaaaacc gccgcggcag tcgcgacgtc cacggtttcg gtgaacggcg ccaactacgc ggtggcggga agcggcccgg ccagcacggt cagcgtgggt gcgccgggca gcgagcgcac gatcaccaat gtggccgcgg gtcaagtaag cgccggttcc accgatgcgg tgaatggttc ggaactgtac gcgacgaacc aggcaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BupsA.01663.c.A2.GE26296
| HARVESTED ON: | 3/10/2009 |
| SEQUENCED ON: | 3/26/2009 |
| EXPECTED MW: | 18kDa |
| OBSERVED MW: | 15kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 69 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGAVAIGNA NIATGTGSLA FGNTSTAAAA GAVALGAGAI ANNANDVALG SASVTAAANP VASALIAGQA YSLAGGAPAS VVSVGAPGAE RQITNVAAGR ISATSTDAVN GSQMNAM
Validated NT Sequence
agtcatcgca ttcatctgcg agccgttcac cgcatcggtc gacgtggcgg aaatccgccc ggccgcgacg ttggtgattt gccgttcggc gccgggcgcg ccgacgctca ccacgctcgc cggcgcgccg ccggcaagcg aataagcctg acctgcgatc aacgcgctgg ccaccggatt cgcagccgcg gtcacgctag cggaacccag cgcgacatcg ttcgcattgt tcgcgattgc gccggcgccc aacgcgaccg cgcccgccgc tgccgccgtc gacgtgttgc cgaacgcaag cgagcccgtt ccggttgcga tgttcgcgtt gccgatcgcg acggcgccca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAVAIGQA AVAKGGLAVS IGVSNTASGD GAVAIGDPNV ATGTGAVALG ADNSANGQGA VALGNANIAT GTGSLAFGNT STAAAAGAVA LGAGAIANNA NDVALGSASV TAAANPVASA LIAGQAYSLA GGAPASVVSV GAPGAERQIT NVAAGRISAT STDAVNGSQM NAMTQA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgccgtc gcgatcggcc aggccgccgt cgcgaagggc gggctggctg tctcgatcgg ggtgtcgaac acggcgagcg gagacggcgc ggtggcgatc ggcgatccga acgtcgcgac cggcaccggc gcggtcgcgc ttggcgcgga caattcggca aacggccagg gcgccgtcgc gctcggcaac gcgaacatcg caaccggaac gggctcgctt gcgttcggca acacgtcgac ggcggcagcg gcgggcgcgg tcgcgttggg cgccggcgca atcgcgaaca atgcgaacga tgtcgcgctg ggttccgcta gcgtgaccgc ggctgcgaat ccggtggcca gcgcgttgat cgcaggtcag gcttattcgc ttgccggcgg cgcgccggcg agcgtggtga gcgtcggcgc gcccggcgcc gaacggcaaa tcaccaacgt cgcggccggg cggatttccg ccacgtcgac cgatgcggtg aacggctcgc agatgaatgc gatgactcag gcgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BupsA.01663.c.A8.GE29407
| HARVESTED ON: | 5/27/2010 |
| SEQUENCED ON: | 6/14/2010 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTGLSTANS NISSLSTGLN SLSTAVNGGG TKYFHANSTQ PDSQALGADS VAVGPAAIAA GASGIAIGNA ANAAANGAVA IGQAAVAKGG LAVSIGVSNT ASGDGAVAIG DPNVATGTGA VALGADNSAN GQGAVALGNA NIATGTGSLA FGNTSTAAAA GAVALGAGAI ANNANDVALG SASVTAAANP VASALIAGQA YSLAGGAPAS VVSVGAPGAE RQITNVAAGR ISATSTDAVN GSQMNAMTQA LESLSTSTAS A
Validated NT Sequence
ttattacgcg ctggccgtcg aagtcgacag cgattccagc gcctgagtca tcgcattcat ctgcgagccg ttcaccgcat cggtcgacgt ggcggaaatc cgcccggccg cgacgttggt gatttgccgt tcggcgccgg gcgcgccgac gctcaccacg ctcgccggcg cgccgccggc aagcgaataa gcctgacctg cgatcaacgc gctggccacc ggattcgcag ccgcggtcac gctagcggaa cccagcgcga catcgttcgc attgttcgcg attgcgccgg cgcccaacgc gaccgcgccc gccgctgccg ccgtcgacgt gttgccgaac gcaagcgagc ccgttccggt tgcgatgttc gcgttgccga gcgcgacggc gccctggccg tttgccgaat tgtccgcgcc aagcgcgacc gcgccggtgc cggtcgcgac gttcggatcg ccgatcgcca ccgcgccgtc tccgctcgcc gtgttcgaca ccccgatcga gacagccagc ccgcccttcg cgacggcggc ctggccgatc gcgacggcgc cgtttgcggc cgcgttcgcc gcattgccga tcgcaatgcc gcttgcgccc gccgcgatgg ccgccggccc gaccgcgacg gaatccgccc ccagcgcctg actgtccggc tgcgtcgagt tggcgtggaa gtacttcgtc ccgccgccgt tgaccgcggt cgacaacgaa ttcagcccgg tggacagcga cgagatgttg ctgttggccg tactcaagcc ggtcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTGLSTANS NISSLSTGLN SLSTAVNGGG TKYFHANSTQ PDSQALGADS VAVGPAAIAA GASGIAIGNA ANAAANGAVA IGQAAVAKGG LAVSIGVSNT ASGDGAVAIG DPNVATGTGA VALGADNSAN GQGAVALGNA NIATGTGSLA FGNTSTAAAA GAVALGAGAI ANNANDVALG SASVTAAANP VASALIAGQA YSLAGGAPAS VVSVGAPGAE RQITNVAAGR ISATSTDAVN GSQMNAMTQA LESLSTSTAS A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccggcttg agtacggcca acagcaacat ctcgtcgctg tccaccgggc tgaattcgtt gtcgaccgcg gtcaacggcg gcgggacgaa gtacttccac gccaactcga cgcagccgga cagtcaggcg ctgggggcgg attccgtcgc ggtcgggccg gcggccatcg cggcgggcgc aagcggcatt gcgatcggca atgcggcgaa cgcggccgca aacggcgccg tcgcgatcgg ccaggccgcc gtcgcgaagg gcgggctggc tgtctcgatc ggggtgtcga acacggcgag cggagacggc gcggtggcga tcggcgatcc gaacgtcgcg accggcaccg gcgcggtcgc gcttggcgcg gacaattcgg caaacggcca gggcgccgtc gcgctcggca acgcgaacat cgcaaccgga acgggctcgc ttgcgttcgg caacacgtcg acggcggcag cggcgggcgc ggtcgcgttg ggcgccggcg caatcgcgaa caatgcgaac gatgtcgcgc tgggttccgc tagcgtgacc gcggctgcga atccggtggc cagcgcgttg atcgcaggtc aggcttattc gcttgccggc ggcgcgccgg cgagcgtggt gagcgtcggc gcgcccggcg ccgaacggca aatcaccaac gtcgcggccg ggcggatttc cgccacgtcg accgatgcgg tgaacggctc gcagatgaat gcgatgactc aggcgctgga atcgctgtcg acttcgacgg ccagcgcgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BupsA.01663.c.A9.GE29408
| HARVESTED ON: | data unavailable |
| SEQUENCED ON: | 6/14/2010 |
| EXPECTED MW: | 18kDa |
| OBSERVED MW: | 24kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSLSTAVSG GGVRTSSLGD TSAGNGANAS GGNGTAVGGA ASASGTDATA LGQASNASGN HSTALGQASS ASGSGSTAVG QGAGAPGDGA SAFGQGALAS GTDSTALGAH STAAAPNSAA IGANSVASAP NSVSFGSRGH ERRLTNVAPG IDGTDAANMN QLWGVQSSVD QAARR
Validated NT Sequence
ttattagcgg cgcgccgcct gatcgacgct cgattgcacg ccccagagct ggttcatgtt cgccgcgtcg gtgccgtcga tccccggcgc gacattcgtc agcctgcgct catggccccg cgaaccgaac gacaccgaat tcggcgcgga cgccaccgaa ttcgcgccga tcgccgccga gttcggcgcc gcagccgtcg aatgagcgcc gagcgccgtc gagtccgtac cggaggcaag tgccccttgg ccgaatgccg aagcgccgtc gccgggcgcg ccggcgcctt gtcccaccgc ggtggagccg cttccggacg cgctcgatgc ttgccccaat gcggtcgaat gattgcccga cgcgttcgac gcctggccca gcgcggtcgc atcggttccc gaagcggacg cggcgccgcc gaccgccgtg ccgttgccgc cggacgcgtt cgcgccattg cccgccgacg tgtcgcccaa gctgctggtg cgaacaccgc cgccggacac ggcggtcgac agcgacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSLSTAVSG GGVRTSSLGD TSAGNGANAS GGNGTAVGGA ASASGTDATA LGQASNASGN HSTALGQASS ASGSGSTAVG QGAGAPGDGA SAFGQGALAS GTDSTALGAH STAAAPNSAA IGANSVASAP NSVSFGSRGH ERRLTNVAPG IDGTDAANMN QLWGVQSSVD QAARR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgctgtcg accgccgtgt ccggcggcgg tgttcgcacc agcagcttgg gcgacacgtc ggcgggcaat ggcgcgaacg cgtccggcgg caacggcacg gcggtcggcg gcgccgcgtc cgcttcggga accgatgcga ccgcgctggg ccaggcgtcg aacgcgtcgg gcaatcattc gaccgcattg gggcaagcat cgagcgcgtc cggaagcggc tccaccgcgg tgggacaagg cgccggcgcg cccggcgacg gcgcttcggc attcggccaa ggggcacttg cctccggtac ggactcgacg gcgctcggcg ctcattcgac ggctgcggcg ccgaactcgg cggcgatcgg cgcgaattcg gtggcgtccg cgccgaattc ggtgtcgttc ggttcgcggg gccatgagcg caggctgacg aatgtcgcgc cggggatcga cggcaccgac gcggcgaaca tgaaccagct ctggggcgtg caatcgagcg tcgatcaggc ggcgcgccgc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BupsA.01663.c.A1.PS00198b
| PURIFICATION DATe: | 4/16/2009 |
| CONCENTRATION: | 10.4mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMGAVAIG KSASAAGGQA VSIGVANTAS GNGAVAIGDP NVATGTGAVA LGNNNTANGQ GAVALGNVST AVGQGSVALG NSSNAAAAGG VALGDTASAV MAGGLALGSL ATASNANDVA LGAGSKTAAA VATSTVSVNG ANYAVAGSGP ASTVSVGAPG SERTITNVAA GQVSAGSTDA VNGSELYAT
Validated NT Sequence
tcgtcgcgta cagttccgaa ccattcaccg catcggtgga accggcgctt acttgacccg cggccacatt ggtgatcgtg cgctcgctgc ccggcgcacc cacgctgacc gtgctggccg ggccgcttcc cgccaccgcg tagttggcgc cgttcaccga aaccgtggac gtcgcgactg ccgcggcggt tttcgaaccc gcgccgagcg ccacgtcgtt cgcattgctc gccgtcgcga gcgagccgag tgccaagccg cccgccatca ccgcgctcgc ggtatcgccc aaggccactc cgcccgccgc ggccgcattg ctgctgttgc cgagcgccac gctgccttgg ccgaccgccg tgctgacgtt gcccagcgcc accgcgcctt gaccattggc cgtattgttg ttgcccagcg cgacagcgcc ggttccggtc gcgacgttcg gatcgccgat cgccaccgcg ccgttgcccg acgcggtatt ggccacgccg atcgaaacgg cttgcccgcc ggcggcggac gccgatttgc cgatggccac cgcgcccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAVAIGKS ASAAGGQAVS IGVANTASGN GAVAIGDPNV ATGTGAVALG NNNTANGQGA VALGNVSTAV GQGSVALGNS SNAAAAGGVA LGDTASAVMA GGLALGSLAT ASNANDVALG AGSKTAAAVA TSTVSVNGAN YAVAGSGPAS TVSVGAPGSE RTITNVAAGQ VSAGSTDAVN GSELYATNQA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCGCGGTG GCCATCGGCA AATCGGCGTC CGCCGCCGGC GGGCAAGCCG TTTCGATCGG CGTGGCCAAT ACCGCGTCGG GCAACGGCGC GGTGGCGATC GGCGATCCGA ACGTCGCGAC CGGAACCGGC GCTGTCGCGC TGGGCAACAA CAATACGGCC AATGGTCAAG GCGCGGTGGC GCTGGGCAAC GTCAGCACGG CGGTCGGCCA AGGCAGCGTG GCGCTCGGCA ACAGCAGCAA TGCGGCCGCG GCGGGCGGAG TGGCCTTGGG CGATACCGCG AGCGCGGTGA TGGCGGGCGG CTTGGCACTC GGCTCGCTCG CGACGGCGAG CAATGCGAAC GACGTGGCGC TCGGCGCGGG TTCGAAAACC GCCGCGGCAG TCGCGACGTC CACGGTTTCG GTGAACGGCG CCAACTACGC GGTGGCGGGA AGCGGCCCGG CCAGCACGGT CAGCGTGGGT GCGCCGGGCA GCGAGCGCAC GATCACCAAT GTGGCCGCGG GTCAAGTAAG CGCCGGTTCC ACCGATGCGG TGAATGGTTC GGAACTGTAC GCGACGAACC AGGCAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA
Details for BupsA.01663.c.A2.PS00264
| PURIFICATION DATe: | 4/16/2009 |
| CONCENTRATION: | 43.5mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 69 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGAVAIGNA NIATGTGSLA FGNTSTAAAA GAVALGAGAI ANNANDVALG SASVTAAANP VASALIAGQA YSLAGGAPAS VVSVGAPGAE RQITNVAAGR ISATSTDAVN GSQMNAM
Validated NT Sequence
agtcatcgca ttcatctgcg agccgttcac cgcatcggtc gacgtggcgg aaatccgccc ggccgcgacg ttggtgattt gccgttcggc gccgggcgcg ccgacgctca ccacgctcgc cggcgcgccg ccggcaagcg aataagcctg acctgcgatc aacgcgctgg ccaccggatt cgcagccgcg gtcacgctag cggaacccag cgcgacatcg ttcgcattgt tcgcgattgc gccggcgccc aacgcgaccg cgcccgccgc tgccgccgtc gacgtgttgc cgaacgcaag cgagcccgtt ccggttgcga tgttcgcgtt gccgatcgcg acggcgccca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAVAIGQA AVAKGGLAVS IGVSNTASGD GAVAIGDPNV ATGTGAVALG ADNSANGQGA VALGNANIAT GTGSLAFGNT STAAAAGAVA LGAGAIANNA NDVALGSASV TAAANPVASA LIAGQAYSLA GGAPASVVSV GAPGAERQIT NVAAGRISAT STDAVNGSQM NAMTQA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCGCCGTC GCGATCGGCC AGGCCGCCGT CGCGAAGGGC GGGCTGGCTG TCTCGATCGG GGTGTCGAAC ACGGCGAGCG GAGACGGCGC GGTGGCGATC GGCGATCCGA ACGTCGCGAC CGGCACCGGC GCGGTCGCGC TTGGCGCGGA CAATTCGGCA AACGGCCAGG GCGCCGTCGC GCTCGGCAAC GCGAACATCG CAACCGGAAC GGGCTCGCTT GCGTTCGGCA ACACGTCGAC GGCGGCAGCG GCGGGCGCGG TCGCGTTGGG CGCCGGCGCA ATCGCGAACA ATGCGAACGA TGTCGCGCTG GGTTCCGCTA GCGTGACCGC GGCTGCGAAT CCGGTGGCCA GCGCGTTGAT CGCAGGTCAG GCTTATTCGC TTGCCGGCGG CGCGCCGGCG AGCGTGGTGA GCGTCGGCGC GCCCGGCGCC GAACGGCAAA TCACCAACGT CGCGGCCGGG CGGATTTCCG CCACGTCGAC CGATGCGGTG AACGGCTCGC AGATGAATGC GATGACTCAG GCGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA